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The map label for this gene is acsA1 [H]

Identifier: 108801663

GI number: 108801663

Start: 4980224

End: 4982155

Strand: Reverse

Name: acsA1 [H]

Synonym: Mmcs_4700

Alternate gene names: 108801663

Gene position: 4982155-4980224 (Counterclockwise)

Preceding gene: 108801664

Following gene: 108801656

Centisome position: 87.32

GC content: 69.82

Gene sequence:

>1932_bases
GTGAGCGAACCGCAGTGGACGCCGACCGAGGACGACATCGCCGGCGCTCGCGTCACCGACTTCGCCCGCTTCGTCCAGCA
GCGCACCGGCGCCGACGTATCCGACTACCACGCGCTGTGGCGGTGGTCGGTCGAGGACCTCGCCGGGTTCTGGGGTGCGC
TGTGGGAGTACTTCGACCTCGGTACTGCGCCCGCCGAAGTGCTCGCCGACGATGCGATGCCCGGTGCGCGGTGGTTCCCG
GGGACACAGCTCAACTACGCCGACCAGGTCGTCCGGCACGCCCGCACCGACCGGCCCGCCATCCTCTACGTCGCCGAGGG
TGGTGACGTCACCGAGATCTCGTGGGATGAGATGCTCACGCGGGCAGCCGGTTTCGCGCGCACGCTGCGGTCGCTGGGTG
TCGGGCCGGGTGACCGCGTCGCGGGGTATCTGCCTAATGTGCCCGAGGCGGTCATCGCCTTCCTCGCCACCGCCAGCATC
GGGGCGATCTGGAGCGCGTGCGGACAGGACTACACCGCGAAGGCCGCGCTGGACCGGCTCGGCCAACTCGAACCGGTGGT
GCTGATCGCCGCAGACGGGTACCGCTTCGGCGGGAAGACACACGACAAGACCGCCGACGTCACCGCGTTGCGCGCCGGGT
TGCCGACGGTGCGGGCCGCGGTGCTGGTGCCGCGGATCGGGGTGGGCGCGCCCGACGCCGACTGGCTGCACTGGGCCGAG
GTCACCGCCGACGGCGGACGGCTGCAACCGGTCCCCGTGGACTTCGACCACCCGCTGTGGGTGCTGTACTCGTCCGGGAC
GACGGGCCTGCCGAAGGGCATCGTGCACGGCCACGGCGGGGTCGTGGTCGAACACCTCAAAGCCGCTGCGCTGCAATTCG
ACATCGGCGCCGACGACACCTTCTTCTGGTTCACCAGCCCGAGCTGGATGATGTGGAACTTTCAGGTGGCAGGGCTGCTG
GTCGGCGCGACCATCGTCTGCTACGACGGCAGCCCGACCTACCCCGCGACCGACACGCTGTGGGAGATCGCGGCCCGGGT
GGGCGCGACGGTGCTGGGCACCAGCCCCGGCTATGTGCTGGCCTGTGACAAGGCGGGTTCCGTCCCGCGCGAGGAGCACG
ATCTGTCCGCGCTGCGCACCGTCGGGATCACCGGTTCGTCGCTGCCCCCGGCCTCGGCACTGTGGCTGCGCGACAACGTC
GGCGTGCAGGTCGCCTCGATCAGCGGCGGCACCGACGTGGTGTCGGCGTTCATCGGCGGTGTGCGGACCGTTCCGGTCTG
GCCCGGTGAGCTCTCGGCGCCCTACCTCGGCGCCGCCGTCGAGGCCTGGGACGAATCCGGTCAGCCGGTCCAGGGTGAGG
TCGGCGAACTCGTCGTCACCAAACCGCTGCCGTCGATGCCGGTGAAGTTCTGGAACGACCCCGACGGAAGCCGTTACCGC
GACGCGTATTTCGAGATGTTCCCGGGCGTCTGGCGGCACGGCGACTGGATCACGATCACCGACCACGGCAGCGTCATCGT
GCACGGCCGGTCGGATTCGACGCTCAACCGGCACGGCATCCGCATGGGGAGTGCGGACATCTACCAGGCCGTCGAGCGGA
TGCCCGAGGTCGCCGAGGCGCTGGTCATCGGGGCCGAGCAACCCGACGGCGGCTACTGGATGCCGCTGTTCGTCACGCTG
ACCGAGGGCGCCGAACTCACCGACGACCTCAAAGACCGGATCTGCCAGACGATCCGCGCCGAGGTGTCACCGCGCCATGT
GCCCGACGACATCATGCTGGCCCCCGGCATCCCGCACACCCGCACCGGTAAGAAACTCGAGGTGCCGATCAAGAAGCTGC
TCCAGGGCGGCGATCCGGCGCGCGTCGTCGAACCGACCGCCGTCGACGATCCGGCCCTTCTGGACTGGTATGTCACGCAG
CGGCGAACCTGA

Upstream 100 bases:

>100_bases
AACCGTCCGAAGTCGGATCGCTGGTGGGCTGGTTGGCCTCGCCACAGTCCGGCATGGTGACCGGTGCGGCCTACACCATG
GACGGCGGATGGAGCGCCCG

Downstream 100 bases:

>100_bases
CGATGTCGATGCTGAGGCCGCCCTCGATGATGCTCCTCGCGTAAACCGGCGGACCCGGTAACGGTGGTGCGGTGGACCGG
TAGGTCGCGCCGGTCGGGGT

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MSEPQWTPTEDDIAGARVTDFARFVQQRTGADVSDYHALWRWSVEDLAGFWGALWEYFDLGTAPAEVLADDAMPGARWFP
GTQLNYADQVVRHARTDRPAILYVAEGGDVTEISWDEMLTRAAGFARTLRSLGVGPGDRVAGYLPNVPEAVIAFLATASI
GAIWSACGQDYTAKAALDRLGQLEPVVLIAADGYRFGGKTHDKTADVTALRAGLPTVRAAVLVPRIGVGAPDADWLHWAE
VTADGGRLQPVPVDFDHPLWVLYSSGTTGLPKGIVHGHGGVVVEHLKAAALQFDIGADDTFFWFTSPSWMMWNFQVAGLL
VGATIVCYDGSPTYPATDTLWEIAARVGATVLGTSPGYVLACDKAGSVPREEHDLSALRTVGITGSSLPPASALWLRDNV
GVQVASISGGTDVVSAFIGGVRTVPVWPGELSAPYLGAAVEAWDESGQPVQGEVGELVVTKPLPSMPVKFWNDPDGSRYR
DAYFEMFPGVWRHGDWITITDHGSVIVHGRSDSTLNRHGIRMGSADIYQAVERMPEVAEALVIGAEQPDGGYWMPLFVTL
TEGAELTDDLKDRICQTIRAEVSPRHVPDDIMLAPGIPHTRTGKKLEVPIKKLLQGGDPARVVEPTAVDDPALLDWYVTQ
RRT

Sequences:

>Translated_643_residues
MSEPQWTPTEDDIAGARVTDFARFVQQRTGADVSDYHALWRWSVEDLAGFWGALWEYFDLGTAPAEVLADDAMPGARWFP
GTQLNYADQVVRHARTDRPAILYVAEGGDVTEISWDEMLTRAAGFARTLRSLGVGPGDRVAGYLPNVPEAVIAFLATASI
GAIWSACGQDYTAKAALDRLGQLEPVVLIAADGYRFGGKTHDKTADVTALRAGLPTVRAAVLVPRIGVGAPDADWLHWAE
VTADGGRLQPVPVDFDHPLWVLYSSGTTGLPKGIVHGHGGVVVEHLKAAALQFDIGADDTFFWFTSPSWMMWNFQVAGLL
VGATIVCYDGSPTYPATDTLWEIAARVGATVLGTSPGYVLACDKAGSVPREEHDLSALRTVGITGSSLPPASALWLRDNV
GVQVASISGGTDVVSAFIGGVRTVPVWPGELSAPYLGAAVEAWDESGQPVQGEVGELVVTKPLPSMPVKFWNDPDGSRYR
DAYFEMFPGVWRHGDWITITDHGSVIVHGRSDSTLNRHGIRMGSADIYQAVERMPEVAEALVIGAEQPDGGYWMPLFVTL
TEGAELTDDLKDRICQTIRAEVSPRHVPDDIMLAPGIPHTRTGKKLEVPIKKLLQGGDPARVVEPTAVDDPALLDWYVTQ
RRT
>Mature_642_residues
SEPQWTPTEDDIAGARVTDFARFVQQRTGADVSDYHALWRWSVEDLAGFWGALWEYFDLGTAPAEVLADDAMPGARWFPG
TQLNYADQVVRHARTDRPAILYVAEGGDVTEISWDEMLTRAAGFARTLRSLGVGPGDRVAGYLPNVPEAVIAFLATASIG
AIWSACGQDYTAKAALDRLGQLEPVVLIAADGYRFGGKTHDKTADVTALRAGLPTVRAAVLVPRIGVGAPDADWLHWAEV
TADGGRLQPVPVDFDHPLWVLYSSGTTGLPKGIVHGHGGVVVEHLKAAALQFDIGADDTFFWFTSPSWMMWNFQVAGLLV
GATIVCYDGSPTYPATDTLWEIAARVGATVLGTSPGYVLACDKAGSVPREEHDLSALRTVGITGSSLPPASALWLRDNVG
VQVASISGGTDVVSAFIGGVRTVPVWPGELSAPYLGAAVEAWDESGQPVQGEVGELVVTKPLPSMPVKFWNDPDGSRYRD
AYFEMFPGVWRHGDWITITDHGSVIVHGRSDSTLNRHGIRMGSADIYQAVERMPEVAEALVIGAEQPDGGYWMPLFVTLT
EGAELTDDLKDRICQTIRAEVSPRHVPDDIMLAPGIPHTRTGKKLEVPIKKLLQGGDPARVVEPTAVDDPALLDWYVTQR
RT

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=651, Percent_Identity=37.3271889400922, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI8923896, Length=658, Percent_Identity=29.1793313069909, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI253970489, Length=671, Percent_Identity=28.3159463487332, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI28416953, Length=620, Percent_Identity=27.9032258064516, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI13375727, Length=625, Percent_Identity=24.96, Blast_Score=171, Evalue=3e-42,
Organism=Homo sapiens, GI157311624, Length=548, Percent_Identity=21.5328467153285, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI157311622, Length=548, Percent_Identity=21.5328467153285, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI58082049, Length=595, Percent_Identity=21.1764705882353, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI122937307, Length=531, Percent_Identity=22.0338983050847, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790505, Length=649, Percent_Identity=26.6563944530046, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1786529, Length=629, Percent_Identity=27.5039745627981, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17569507, Length=637, Percent_Identity=32.3390894819466, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71983001, Length=652, Percent_Identity=28.2208588957055, Blast_Score=211, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI71982997, Length=652, Percent_Identity=28.2208588957055, Blast_Score=211, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6323182, Length=658, Percent_Identity=28.419452887538, Blast_Score=227, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6319264, Length=625, Percent_Identity=29.12, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24667955, Length=644, Percent_Identity=27.0186335403727, Blast_Score=218, Evalue=9e-57,
Organism=Drosophila melanogaster, GI62472339, Length=533, Percent_Identity=28.5178236397749, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24667959, Length=502, Percent_Identity=28.2868525896414, Blast_Score=190, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21355225, Length=630, Percent_Identity=24.9206349206349, Blast_Score=161, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24648260, Length=361, Percent_Identity=24.0997229916898, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 69423; Mature: 69292

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPQWTPTEDDIAGARVTDFARFVQQRTGADVSDYHALWRWSVEDLAGFWGALWEYFDL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GTAPAEVLADDAMPGARWFPGTQLNYADQVVRHARTDRPAILYVAEGGDVTEISWDEMLT
CCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEECHHHHHH
RAAGFARTLRSLGVGPGDRVAGYLPNVPEAVIAFLATASIGAIWSACGQDYTAKAALDRL
HHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GQLEPVVLIAADGYRFGGKTHDKTADVTALRAGLPTVRAAVLVPRIGVGAPDADWLHWAE
CCCCCEEEEEECCEECCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEE
VTADGGRLQPVPVDFDHPLWVLYSSGTTGLPKGIVHGHGGVVVEHLKAAALQFDIGADDT
EECCCCEECCCCCCCCCCEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHEEEECCCCCE
FFWFTSPSWMMWNFQVAGLLVGATIVCYDGSPTYPATDTLWEIAARVGATVLGTSPGYVL
EEEEECCCEEEEEEEEHHEEECCEEEEECCCCCCCCHHHHHHHHHHHCCEEEECCCCEEE
ACDKAGSVPREEHDLSALRTVGITGSSLPPASALWLRDNVGVQVASISGGTDVVSAFIGG
EECCCCCCCCCHHHHHHHHEECCCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHCC
VRTVPVWPGELSAPYLGAAVEAWDESGQPVQGEVGELVVTKPLPSMPVKFWNDPDGSRYR
CEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCEECCCCCCCHHH
DAYFEMFPGVWRHGDWITITDHGSVIVHGRSDSTLNRHGIRMGSADIYQAVERMPEVAEA
HHHHHHCCCHHCCCCEEEEECCCCEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LVIGAEQPDGGYWMPLFVTLTEGAELTDDLKDRICQTIRAEVSPRHVPDDIMLAPGIPHT
HEECCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCC
RTGKKLEVPIKKLLQGGDPARVVEPTAVDDPALLDWYVTQRRT
CCCCEEECCHHHHHCCCCCCEEECCCCCCCCHHEEHHHHCCCC
>Mature Secondary Structure 
SEPQWTPTEDDIAGARVTDFARFVQQRTGADVSDYHALWRWSVEDLAGFWGALWEYFDL
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GTAPAEVLADDAMPGARWFPGTQLNYADQVVRHARTDRPAILYVAEGGDVTEISWDEMLT
CCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEECHHHHHH
RAAGFARTLRSLGVGPGDRVAGYLPNVPEAVIAFLATASIGAIWSACGQDYTAKAALDRL
HHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GQLEPVVLIAADGYRFGGKTHDKTADVTALRAGLPTVRAAVLVPRIGVGAPDADWLHWAE
CCCCCEEEEEECCEECCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEE
VTADGGRLQPVPVDFDHPLWVLYSSGTTGLPKGIVHGHGGVVVEHLKAAALQFDIGADDT
EECCCCEECCCCCCCCCCEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHEEEECCCCCE
FFWFTSPSWMMWNFQVAGLLVGATIVCYDGSPTYPATDTLWEIAARVGATVLGTSPGYVL
EEEEECCCEEEEEEEEHHEEECCEEEEECCCCCCCCHHHHHHHHHHHCCEEEECCCCEEE
ACDKAGSVPREEHDLSALRTVGITGSSLPPASALWLRDNVGVQVASISGGTDVVSAFIGG
EECCCCCCCCCHHHHHHHHEECCCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHCC
VRTVPVWPGELSAPYLGAAVEAWDESGQPVQGEVGELVVTKPLPSMPVKFWNDPDGSRYR
CEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCEECCCCCCCHHH
DAYFEMFPGVWRHGDWITITDHGSVIVHGRSDSTLNRHGIRMGSADIYQAVERMPEVAEA
HHHHHHCCCHHCCCCEEEEECCCCEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LVIGAEQPDGGYWMPLFVTLTEGAELTDDLKDRICQTIRAEVSPRHVPDDIMLAPGIPHT
HEECCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCC
RTGKKLEVPIKKLLQGGDPARVVEPTAVDDPALLDWYVTQRRT
CCCCEEECCHHHHHCCCCCCEEECCCCCCCCHHEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]