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The map label for this gene is glyA [H]

Identifier: 108801095

GI number: 108801095

Start: 4397634

End: 4399112

Strand: Reverse

Name: glyA [H]

Synonym: Mmcs_4131

Alternate gene names: 108801095

Gene position: 4399112-4397634 (Counterclockwise)

Preceding gene: 108801096

Following gene: 108801094

Centisome position: 77.1

GC content: 69.71

Gene sequence:

>1479_bases
ATGACTGCAGACTCCGTGACCTCTTCGGCGGCCGCTCCGGGCGCCGAGTACGCCGCCACCGCGAGCACGGCCTACCAGGC
CGCGCTCCAGGTCATCGAGTCCGTCGAACCCCGGATCGCGGCGGCCACCCGCAAAGAGCTGGCCGATCAACGGGATTCAC
TCAAGCTGATCGCCAGCGAGAACTACGCCTCCCCGGCCGTGCTGCTGACCATGGGCACCTGGCTGAGTGACAAATACGCC
GAGGGCACCATCGGCCACCGGTTCTACGCGGGCTGCCAGAACGTCGACGTCGTCGAGAGCGTCGCCGCCGAACACGCCCG
CGAACTGTTCGGCGCGCCCTACGCCTACGCCCAGCCGCACTCCGGCATCGACGCCAACCTGGTCGCCTTCTGGGCCATCC
TGGCCACCCGCGTCGAGGCGCCCGGACTGGCCGAACTCGGCGCCAAACACGTCAACGACCTGTCCGAGGCGGACTGGGAG
AGCCTGCGCAACAAGCTCGGCAACCAGCGGCTGCTCGGCATGTCGCTCGACGCCGGCGGGCACCTCACCCACGGCTTCCG
CCCCAACATCAGCGGCAAGATGTTCCACCAGCGCAGCTACGGCACCGATCCGGAGACCGGGCTGCTCGACTACGACAAGC
TCGCCGCCGCCGCCCGCGAGTTCAAACCGCTGATCCTCGTCGGCGGCTACTCCGCCTACCCGCGCCGGGTGAACTTCGCC
AAGCTCCGCGAGATCGCCGACGAGGTCGGCGCCACCCTGATGGTCGACATGGCCCACTTCGCCGGTCTGGTCGCCGGCAA
GGTCTTCACCGGTGACGAGGATCCGGTGCCGCACGCCCACGTCGTCACCACCACCACGCACAAATCGCTGCGCGGCCCGC
GCGGCGGTCTGGTGCTGGCCCAACCCGAGTACTCCGACGCCGTCGACAAGGGCTGCCCGATGGTGCTCGGCGGTCCGCTC
TCGCACGTCATGGCCGCCAAGGCGGTCGCGCTCGCCGAGGCGCGTCAGCCGTCGTTCCAGGCCTACGCCCAGCGCGTCGC
CGACAACGCCAAATCGCTCGCCGAGGGTTTCCTCAAGCGGGGCGCCCGGCTGGTCACCGGCGGCACCGACAACCACATCG
TGCTGCTCGACGTGACGAGCTTCGGTCTGACCGGCCGGCAGGCCGAGTCGGCGCTGCTCGACGCCGGCGTCGTGACCAAC
CGCAACTCGATCCCGGCCGACCCCAACGGCGCCTGGTACACCAGCGGCATCCGATTCGGCACCCCGGCACTGACCACCCG
CGGCTTCGGCGCCGCGGAGTTCGACCGGGTCGCCGAACTGGTCGTCGACGTGCTGAACAACACCCAGCCCAATTCGACCG
GACCCACTGGGTTCTCCAAGGCCAAGTACACGCTGGCCGACGGCACCGCCGAACGCGTCCACGCGGCGTCGGCGGAGATG
CTCGACGCCAACCCGCTGTATCCGGGTCTCACGCTCTGA

Upstream 100 bases:

>100_bases
TTCATCCGCGACACGCGGGGTGGGGCGTATCAGTTCGCACACTCGGCGCAGATGGCGGGCGATTTCTGCCCCGGCGCCCG
ACGCGATTACGCTCGACAGC

Downstream 100 bases:

>100_bases
GGCGACGCGCCGCGGGAATTTTACGATTCCCACAGGATTGGGTTACTGTAACCGCATGGCACAGAAACCTGTCCCTAACG
CGCTGACCCTCGAGCTGGAG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTADSVTSSAAAPGAEYAATASTAYQAALQVIESVEPRIAAATRKELADQRDSLKLIASENYASPAVLLTMGTWLSDKYA
EGTIGHRFYAGCQNVDVVESVAAEHARELFGAPYAYAQPHSGIDANLVAFWAILATRVEAPGLAELGAKHVNDLSEADWE
SLRNKLGNQRLLGMSLDAGGHLTHGFRPNISGKMFHQRSYGTDPETGLLDYDKLAAAAREFKPLILVGGYSAYPRRVNFA
KLREIADEVGATLMVDMAHFAGLVAGKVFTGDEDPVPHAHVVTTTTHKSLRGPRGGLVLAQPEYSDAVDKGCPMVLGGPL
SHVMAAKAVALAEARQPSFQAYAQRVADNAKSLAEGFLKRGARLVTGGTDNHIVLLDVTSFGLTGRQAESALLDAGVVTN
RNSIPADPNGAWYTSGIRFGTPALTTRGFGAAEFDRVAELVVDVLNNTQPNSTGPTGFSKAKYTLADGTAERVHAASAEM
LDANPLYPGLTL

Sequences:

>Translated_492_residues
MTADSVTSSAAAPGAEYAATASTAYQAALQVIESVEPRIAAATRKELADQRDSLKLIASENYASPAVLLTMGTWLSDKYA
EGTIGHRFYAGCQNVDVVESVAAEHARELFGAPYAYAQPHSGIDANLVAFWAILATRVEAPGLAELGAKHVNDLSEADWE
SLRNKLGNQRLLGMSLDAGGHLTHGFRPNISGKMFHQRSYGTDPETGLLDYDKLAAAAREFKPLILVGGYSAYPRRVNFA
KLREIADEVGATLMVDMAHFAGLVAGKVFTGDEDPVPHAHVVTTTTHKSLRGPRGGLVLAQPEYSDAVDKGCPMVLGGPL
SHVMAAKAVALAEARQPSFQAYAQRVADNAKSLAEGFLKRGARLVTGGTDNHIVLLDVTSFGLTGRQAESALLDAGVVTN
RNSIPADPNGAWYTSGIRFGTPALTTRGFGAAEFDRVAELVVDVLNNTQPNSTGPTGFSKAKYTLADGTAERVHAASAEM
LDANPLYPGLTL
>Mature_491_residues
TADSVTSSAAAPGAEYAATASTAYQAALQVIESVEPRIAAATRKELADQRDSLKLIASENYASPAVLLTMGTWLSDKYAE
GTIGHRFYAGCQNVDVVESVAAEHARELFGAPYAYAQPHSGIDANLVAFWAILATRVEAPGLAELGAKHVNDLSEADWES
LRNKLGNQRLLGMSLDAGGHLTHGFRPNISGKMFHQRSYGTDPETGLLDYDKLAAAAREFKPLILVGGYSAYPRRVNFAK
LREIADEVGATLMVDMAHFAGLVAGKVFTGDEDPVPHAHVVTTTTHKSLRGPRGGLVLAQPEYSDAVDKGCPMVLGGPLS
HVMAAKAVALAEARQPSFQAYAQRVADNAKSLAEGFLKRGARLVTGGTDNHIVLLDVTSFGLTGRQAESALLDAGVVTNR
NSIPADPNGAWYTSGIRFGTPALTTRGFGAAEFDRVAELVVDVLNNTQPNSTGPTGFSKAKYTLADGTAERVHAASAEML
DANPLYPGLTL

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=436, Percent_Identity=36.697247706422, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI261862350, Length=436, Percent_Identity=36.697247706422, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI261862348, Length=436, Percent_Identity=36.697247706422, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI19923315, Length=436, Percent_Identity=36.9266055045872, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI261862346, Length=432, Percent_Identity=35.8796296296296, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI22547186, Length=437, Percent_Identity=36.3844393592677, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI22547189, Length=421, Percent_Identity=36.5795724465558, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI1788902, Length=422, Percent_Identity=46.9194312796209, Blast_Score=342, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=434, Percent_Identity=37.0967741935484, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI25144732, Length=434, Percent_Identity=37.0967741935484, Blast_Score=271, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6319739, Length=431, Percent_Identity=37.122969837587, Blast_Score=279, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6323087, Length=431, Percent_Identity=35.9628770301624, Blast_Score=256, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24640005, Length=430, Percent_Identity=37.2093023255814, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI221329721, Length=430, Percent_Identity=37.906976744186, Blast_Score=290, Evalue=2e-78,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 52050; Mature: 51919

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00096 SHMT ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADSVTSSAAAPGAEYAATASTAYQAALQVIESVEPRIAAATRKELADQRDSLKLIASE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHCEEEEECC
NYASPAVLLTMGTWLSDKYAEGTIGHRFYAGCQNVDVVESVAAEHARELFGAPYAYAQPH
CCCCCEEEEEECCHHCCHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
SGIDANLVAFWAILATRVEAPGLAELGAKHVNDLSEADWESLRNKLGNQRLLGMSLDAGG
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
HLTHGFRPNISGKMFHQRSYGTDPETGLLDYDKLAAAAREFKPLILVGGYSAYPRRVNFA
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCHH
KLREIADEVGATLMVDMAHFAGLVAGKVFTGDEDPVPHAHVVTTTTHKSLRGPRGGLVLA
HHHHHHHHHCHHHHHHHHHHHHHHHCCEECCCCCCCCCCEEEEECCHHHHCCCCCCEEEE
QPEYSDAVDKGCPMVLGGPLSHVMAAKAVALAEARQPSFQAYAQRVADNAKSLAEGFLKR
CCCHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
GARLVTGGTDNHIVLLDVTSFGLTGRQAESALLDAGVVTNRNSIPADPNGAWYTSGIRFG
CCEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCEEECCCCCCCCCCCCEEECCEEEC
TPALTTRGFGAAEFDRVAELVVDVLNNTQPNSTGPTGFSKAKYTLADGTAERVHAASAEM
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHH
LDANPLYPGLTL
CCCCCCCCCCCC
>Mature Secondary Structure 
TADSVTSSAAAPGAEYAATASTAYQAALQVIESVEPRIAAATRKELADQRDSLKLIASE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHCEEEEECC
NYASPAVLLTMGTWLSDKYAEGTIGHRFYAGCQNVDVVESVAAEHARELFGAPYAYAQPH
CCCCCEEEEEECCHHCCHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
SGIDANLVAFWAILATRVEAPGLAELGAKHVNDLSEADWESLRNKLGNQRLLGMSLDAGG
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
HLTHGFRPNISGKMFHQRSYGTDPETGLLDYDKLAAAAREFKPLILVGGYSAYPRRVNFA
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCHH
KLREIADEVGATLMVDMAHFAGLVAGKVFTGDEDPVPHAHVVTTTTHKSLRGPRGGLVLA
HHHHHHHHHCHHHHHHHHHHHHHHHCCEECCCCCCCCCCEEEEECCHHHHCCCCCCEEEE
QPEYSDAVDKGCPMVLGGPLSHVMAAKAVALAEARQPSFQAYAQRVADNAKSLAEGFLKR
CCCHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
GARLVTGGTDNHIVLLDVTSFGLTGRQAESALLDAGVVTNRNSIPADPNGAWYTSGIRFG
CCEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCEEECCCCCCCCCCCCEEECCEEEC
TPALTTRGFGAAEFDRVAELVVDVLNNTQPNSTGPTGFSKAKYTLADGTAERVHAASAEM
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHH
LDANPLYPGLTL
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA