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The map label for this gene is hemK [H]

Identifier: 108800850

GI number: 108800850

Start: 4138258

End: 4139169

Strand: Reverse

Name: hemK [H]

Synonym: Mmcs_3886

Alternate gene names: 108800850

Gene position: 4139169-4138258 (Counterclockwise)

Preceding gene: 108800851

Following gene: 108800849

Centisome position: 72.55

GC content: 73.79

Gene sequence:

>912_bases
ATGACCCGGGCGCCGGCGTCATCGCTGCGCCAGGTCATCGACGAGGCCGCGGCGGTGCTGGCCGCGGCCGGGGTCGCCTC
GGCTCGGGTGGACGCGGAACTGCTGGCCGCGCACGCGGCGGGCACCGACCGCGGGCGACTGATGTTCGCCGACCTCCCGC
CGGACTTCGGCATCCGCTTCGCCGACCTCGTGTCGGCCCGCGCGAAGCGCATCCCGCTGCAGCACCTCACCGGCACGGCG
GCGTTCGGGCCGGTGCAGGTCAGCGTCGGTCCGGGGGTCTTCATCCCGCGCCCGGAGACCGAGGCGCTCTTCGAATGGGT
TGTCCGCCAGGCACTTCCGGACGAGCCGCTGATCGTCGACCTGTGCACCGGCTCGGGAGCGCTGGCGTTGGCGATCGCGA
CCGCGTTGCCGAAGGCGCGGGTGATCGCCGTCGAGAACTCCGCCGACGCGCTCGGCTACGCCCGCCGCAACCTCGCAGGC
AGCGGCGTGGAACTGCTCGACGCCGACGTCACCGCGGCCGACCTGCGCCCGGACCTCGACGGTGCCGTCGACCTCGTCGT
CGCCAACCCGCCCTACATCCCCGAGGGGGCCGCCCTCGACCCCGAGGTCGCCGAACACGATCCGCCCCAGGCGCTGTTCG
GCGGACCCGACGGCATGGTGGTGATCGACGCGATCGTCGGCCTCGCGCAGCGCTGGCTGCGGTCCGGCGGCCGCGTCGCG
GTCGAACACGACGACACCACCTCGGAACTCACGGTGGCGGCCTTCCGCCGCACCGGCGCTGTTTTCGAGGACGCTGCTCT
CGGGGACGCTGTTTTCGACGACGTGACCACCCACACCGACCTGACCGGCCGCCCGCGCTTCGTCACCGCCCGGCGTCGAC
CGGCCACCACTGCCACCGCGGAGGCGCGATGA

Upstream 100 bases:

>100_bases
ACTTCAAGGCGCACAACCTCGACCAGGTGCTCGACGGCGATCTCGACCCGCTGTTCGACGCGCTGGCGGCCGCCGACAAA
CAGGCGCGACTGCAGAGCTC

Downstream 100 bases:

>100_bases
CCCAACTGTTCGACTGCACCGACCCCGACAACCGGGCGACCGGTATCGCGGCCGCCGTCAGCGCGCTCAAAGACGGCGGG
CTGGTGGTGTTGCCCACCGA

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.MleHemKP [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTRAPASSLRQVIDEAAAVLAAAGVASARVDAELLAAHAAGTDRGRLMFADLPPDFGIRFADLVSARAKRIPLQHLTGTA
AFGPVQVSVGPGVFIPRPETEALFEWVVRQALPDEPLIVDLCTGSGALALAIATALPKARVIAVENSADALGYARRNLAG
SGVELLDADVTAADLRPDLDGAVDLVVANPPYIPEGAALDPEVAEHDPPQALFGGPDGMVVIDAIVGLAQRWLRSGGRVA
VEHDDTTSELTVAAFRRTGAVFEDAALGDAVFDDVTTHTDLTGRPRFVTARRRPATTATAEAR

Sequences:

>Translated_303_residues
MTRAPASSLRQVIDEAAAVLAAAGVASARVDAELLAAHAAGTDRGRLMFADLPPDFGIRFADLVSARAKRIPLQHLTGTA
AFGPVQVSVGPGVFIPRPETEALFEWVVRQALPDEPLIVDLCTGSGALALAIATALPKARVIAVENSADALGYARRNLAG
SGVELLDADVTAADLRPDLDGAVDLVVANPPYIPEGAALDPEVAEHDPPQALFGGPDGMVVIDAIVGLAQRWLRSGGRVA
VEHDDTTSELTVAAFRRTGAVFEDAALGDAVFDDVTTHTDLTGRPRFVTARRRPATTATAEAR
>Mature_302_residues
TRAPASSLRQVIDEAAAVLAAAGVASARVDAELLAAHAAGTDRGRLMFADLPPDFGIRFADLVSARAKRIPLQHLTGTAA
FGPVQVSVGPGVFIPRPETEALFEWVVRQALPDEPLIVDLCTGSGALALAIATALPKARVIAVENSADALGYARRNLAGS
GVELLDADVTAADLRPDLDGAVDLVVANPPYIPEGAALDPEVAEHDPPQALFGGPDGMVVIDAIVGLAQRWLRSGGRVAV
EHDDTTSELTVAAFRRTGAVFEDAALGDAVFDDVTTHTDLTGRPRFVTARRRPATTATAEAR

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=310, Percent_Identity=30.6451612903226, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1787463, Length=296, Percent_Identity=33.445945945946, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082085, Length=177, Percent_Identity=34.4632768361582, Blast_Score=82, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324266, Length=204, Percent_Identity=28.921568627451, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24582226, Length=248, Percent_Identity=29.8387096774194, Blast_Score=104, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31573; Mature: 31442

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAPASSLRQVIDEAAAVLAAAGVASARVDAELLAAHAAGTDRGRLMFADLPPDFGIRF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHH
ADLVSARAKRIPLQHLTGTAAFGPVQVSVGPGVFIPRPETEALFEWVVRQALPDEPLIVD
HHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCEEEE
LCTGSGALALAIATALPKARVIAVENSADALGYARRNLAGSGVELLDADVTAADLRPDLD
EECCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCHHHCCCCCC
GAVDLVVANPPYIPEGAALDPEVAEHDPPQALFGGPDGMVVIDAIVGLAQRWLRSGGRVA
CCEEEEECCCCCCCCCCCCCCHHHCCCCCHHHCCCCCCEEHHHHHHHHHHHHHHCCCEEE
VEHDDTTSELTVAAFRRTGAVFEDAALGDAVFDDVTTHTDLTGRPRFVTARRRPATTATA
EECCCCCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCC
EAR
CCC
>Mature Secondary Structure 
TRAPASSLRQVIDEAAAVLAAAGVASARVDAELLAAHAAGTDRGRLMFADLPPDFGIRF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHH
ADLVSARAKRIPLQHLTGTAAFGPVQVSVGPGVFIPRPETEALFEWVVRQALPDEPLIVD
HHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCEEEE
LCTGSGALALAIATALPKARVIAVENSADALGYARRNLAGSGVELLDADVTAADLRPDLD
EECCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCHHHCCCCCC
GAVDLVVANPPYIPEGAALDPEVAEHDPPQALFGGPDGMVVIDAIVGLAQRWLRSGGRVA
CCEEEEECCCCCCCCCCCCCCHHHCCCCCHHHCCCCCCEEHHHHHHHHHHHHHHCCCEEE
VEHDDTTSELTVAAFRRTGAVFEDAALGDAVFDDVTTHTDLTGRPRFVTARRRPATTATA
EECCCCCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCC
EAR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]