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The map label for this gene is prfA

Identifier: 108800851

GI number: 108800851

Start: 4139166

End: 4140239

Strand: Reverse

Name: prfA

Synonym: Mmcs_3887

Alternate gene names: 108800851

Gene position: 4140239-4139166 (Counterclockwise)

Preceding gene: 108800852

Following gene: 108800850

Centisome position: 72.57

GC content: 69.09

Gene sequence:

>1074_bases
ATGAGTGCTCCCACCACAGCGATTGACGCGTTGCTCGCCGAGCACGCCGACCTGGAGCGTCAGCTCGCCGACCCCGCGCT
GCACGCCGACGCCGGCAAGGCGCGCAAGGCCGGTCGCCGGTTCGCGCAGCTGGCGCCGATCGTCGCCACGTACCGAAAAC
TCGAAGCCGCCCGCGGTGACCTCGAGGCGGCCCGTGAGCTCGGCGCCGACGATGCGTCGTTCGCCGCCGAGGTGCCCGAA
CTCGAGGCCACCGTCGACCAACTCGAGACCCAGCTGTCGGATCTGCTGGCGCCGCGTGATCCGCACGACGCCGACGACAT
CGTGCTCGAGGTGAAGTCGGGGGAGGGCGGCGAGGAGTCGGCCCTGTTCGCCGCCGACCTCGCGCGGATGTACATCCGGT
ACGCCGAACGGCACGGCTGGTCGGTCACGATCCTCGACGAGACGACGTCCGACCTCGGTGGATACAAGGACGCAACGCTC
TCGATCCGCAGCAAGGGCGACTCGGCCGACGGGGTGTGGTCGCGGCTGAAGTTCGAAGGCGGCGTGCACCGCGTGCAACG
GGTGCCGGTCACCGAATCGCAGGGCCGCGTCCACACGTCGGCGGCCGGCGTGCTCGTCTACCCCGAACCCGAAGAGGTCG
AACAGGTCCAGATCGACGAGTCCGACCTGCGCATCGACGTCTACCGGTCCTCGGGTAAGGGCGGTCAGGGCGTCAACACC
ACCGACTCGGCCGTGCGGATCACCCACCTGCCGACCGGCATCGTCGTCACCTGCCAGAACGAACGCTCTCAGCTGCAGAA
CAAGGCGCGTGCCATGCAGGTGCTCGCCGCCCGGTTGCAGTCGCTCGCCGAGGAGCAGGCCTCGGCCGATGCGTCGGCCG
ACCGCGCCAGCCAGATCCGCACCGTCGACCGCAGCGAGCGGATCCGTACGTACAACTTCCCGGAGAACCGGATCGCCGAC
CACCGCATCAACTTCAAGGCGCACAACCTCGACCAGGTGCTCGACGGCGATCTCGACCCGCTGTTCGACGCGCTGGCGGC
CGCCGACAAACAGGCGCGACTGCAGAGCTCATGA

Upstream 100 bases:

>100_bases
TGCCGACAAGTAGCTACCACTCCGACGCCCGCTCTGCCGCCTCGCGCGCAGGCCGGGCGTCGGTTTGCGTTCGGGGAAGT
CTTCGGGGAAGGAGGTCGCC

Downstream 100 bases:

>100_bases
CCCGGGCGCCGGCGTCATCGCTGCGCCAGGTCATCGACGAGGCCGCGGCGGTGCTGGCCGCGGCCGGGGTCGCCTCGGCT
CGGGTGGACGCGGAACTGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSAPTTAIDALLAEHADLERQLADPALHADAGKARKAGRRFAQLAPIVATYRKLEAARGDLEAARELGADDASFAAEVPE
LEATVDQLETQLSDLLAPRDPHDADDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWSVTILDETTSDLGGYKDATL
SIRSKGDSADGVWSRLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEQVQIDESDLRIDVYRSSGKGGQGVNT
TDSAVRITHLPTGIVVTCQNERSQLQNKARAMQVLAARLQSLAEEQASADASADRASQIRTVDRSERIRTYNFPENRIAD
HRINFKAHNLDQVLDGDLDPLFDALAAADKQARLQSS

Sequences:

>Translated_357_residues
MSAPTTAIDALLAEHADLERQLADPALHADAGKARKAGRRFAQLAPIVATYRKLEAARGDLEAARELGADDASFAAEVPE
LEATVDQLETQLSDLLAPRDPHDADDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWSVTILDETTSDLGGYKDATL
SIRSKGDSADGVWSRLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEQVQIDESDLRIDVYRSSGKGGQGVNT
TDSAVRITHLPTGIVVTCQNERSQLQNKARAMQVLAARLQSLAEEQASADASADRASQIRTVDRSERIRTYNFPENRIAD
HRINFKAHNLDQVLDGDLDPLFDALAAADKQARLQSS
>Mature_356_residues
SAPTTAIDALLAEHADLERQLADPALHADAGKARKAGRRFAQLAPIVATYRKLEAARGDLEAARELGADDASFAAEVPEL
EATVDQLETQLSDLLAPRDPHDADDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWSVTILDETTSDLGGYKDATLS
IRSKGDSADGVWSRLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEQVQIDESDLRIDVYRSSGKGGQGVNTT
DSAVRITHLPTGIVVTCQNERSQLQNKARAMQVLAARLQSLAEEQASADASADRASQIRTVDRSERIRTYNFPENRIADH
RINFKAHNLDQVLDGDLDPLFDALAAADKQARLQSS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=271, Percent_Identity=48.7084870848708, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI34577120, Length=369, Percent_Identity=39.0243902439024, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI166795305, Length=176, Percent_Identity=48.8636363636364, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1787462, Length=344, Percent_Identity=47.6744186046512, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=38.0952380952381, Blast_Score=200, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=315, Percent_Identity=34.9206349206349, Blast_Score=161, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6321295, Length=358, Percent_Identity=38.8268156424581, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI19921226, Length=339, Percent_Identity=38.9380530973451, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_MYCSJ (A3Q3C2)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001072140.1
- ProteinModelPortal:   A3Q3C2
- SMR:   A3Q3C2
- STRING:   A3Q3C2
- EnsemblBacteria:   EBMYCT00000050777
- GeneID:   4879583
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_3873
- eggNOG:   COG0216
- GeneTree:   EBGT00050000015865
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   A3Q3C2
- ProtClustDB:   PRK00591
- BioCyc:   MSP164757:MJLS_3873-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 38831; Mature: 38700

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTTAIDALLAEHADLERQLADPALHADAGKARKAGRRFAQLAPIVATYRKLEAARGD
CCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
LEAARELGADDASFAAEVPELEATVDQLETQLSDLLAPRDPHDADDIVLEVKSGEGGEES
HHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHH
ALFAADLARMYIRYAERHGWSVTILDETTSDLGGYKDATLSIRSKGDSADGVWSRLKFEG
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHCC
GVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEQVQIDESDLRIDVYRSSGKGGQGVNT
CHHHHHCCCCCCCCCCEEECCCEEEECCCCCHHHEEEECCCCCEEEEEECCCCCCCCCCC
TDSAVRITHLPTGIVVTCQNERSQLQNKARAMQVLAARLQSLAEEQASADASADRASQIR
CCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TVDRSERIRTYNFPENRIADHRINFKAHNLDQVLDGDLDPLFDALAAADKQARLQSS
HCCHHCCCEECCCCCHHCCCCEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAPTTAIDALLAEHADLERQLADPALHADAGKARKAGRRFAQLAPIVATYRKLEAARGD
CCCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
LEAARELGADDASFAAEVPELEATVDQLETQLSDLLAPRDPHDADDIVLEVKSGEGGEES
HHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHH
ALFAADLARMYIRYAERHGWSVTILDETTSDLGGYKDATLSIRSKGDSADGVWSRLKFEG
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHCC
GVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEQVQIDESDLRIDVYRSSGKGGQGVNT
CHHHHHCCCCCCCCCCEEECCCEEEECCCCCHHHEEEECCCCCEEEEEECCCCCCCCCCC
TDSAVRITHLPTGIVVTCQNERSQLQNKARAMQVLAARLQSLAEEQASADASADRASQIR
CCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TVDRSERIRTYNFPENRIADHRINFKAHNLDQVLDGDLDPLFDALAAADKQARLQSS
HCCHHCCCEECCCCCHHCCCCEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA