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The map label for this gene is hisH [H]

Identifier: 108800026

GI number: 108800026

Start: 3236124

End: 3236741

Strand: Reverse

Name: hisH [H]

Synonym: Mmcs_3060

Alternate gene names: 108800026

Gene position: 3236741-3236124 (Counterclockwise)

Preceding gene: 108800027

Following gene: 108800025

Centisome position: 56.73

GC content: 71.36

Gene sequence:

>618_bases
GTGAGCAGGAAAGTCGTCATCCTCGACTACGGCTCGGGCAATCTGCGCTCGGCGCAGCGCGCGGTCGAGCGCACCGGTGC
CGACGTCGAGGTCACCGCCGACGCCGACGCCGCGCTGAACGCCGACGGTCTGGTGGTGCCGGGCGTCGGGGCGTTCGAGG
CATGTATGGCCGGTCTGCGCGAGATCGGCGGGGAGAAGGTCATCGCCGAGCGGGTCGCCGCGGGGCGACCGGTCCTCGGC
GTGTGCGTGGGCATGCAGATCCTCTTCGCGCGCGGCGTCGAATTCGGTGTCGAGACCGCCGGATGCGGGCAATGGCCCGG
ATCGGTGGTGCGGCTCGACGCGCCGGTGATCCCGCACATGGGCTGGAACGTCGTCGACGCCCCGGCCGGGAGCACACTGT
TCCGCGGAATCGACCCCGACACCCGCTTCTACTTCGTGCACTCCTACGCCGCGCAGCAGTGGGAGGGCGACCCGTCCGCG
CGCCTCACCTGGGCGACGCACCACGTCCCGTTCCTCGCCGCCGTCGAGGATGGTCCGCTCTCGGCCACCCAGTTCCACCC
GGAGAAGAGCGGTGACGCGGGCGCGGCACTGCTCCGCAACTGGGTCGAGGAGCTCTGA

Upstream 100 bases:

>100_bases
ACATCACCGAGGCGCAGTACAAGGCCGTCGCGCGCGCGTTGCGCCAGGCCGTCGAACTCGATCCGCGGGTGACGGGTGTG
CCGTCCACGAAAGGCTCACT

Downstream 100 bases:

>100_bases
TCTAAGGTTGTCCGTCGTGCCAGAGAAGTCGGTGTCCGAGAAAAGACCGTTGATCCTCCTCCCCGCCGTCGATGTCGTCG
AAGGCCGTGCCGTGCGCCTG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MSRKVVILDYGSGNLRSAQRAVERTGADVEVTADADAALNADGLVVPGVGAFEACMAGLREIGGEKVIAERVAAGRPVLG
VCVGMQILFARGVEFGVETAGCGQWPGSVVRLDAPVIPHMGWNVVDAPAGSTLFRGIDPDTRFYFVHSYAAQQWEGDPSA
RLTWATHHVPFLAAVEDGPLSATQFHPEKSGDAGAALLRNWVEEL

Sequences:

>Translated_205_residues
MSRKVVILDYGSGNLRSAQRAVERTGADVEVTADADAALNADGLVVPGVGAFEACMAGLREIGGEKVIAERVAAGRPVLG
VCVGMQILFARGVEFGVETAGCGQWPGSVVRLDAPVIPHMGWNVVDAPAGSTLFRGIDPDTRFYFVHSYAAQQWEGDPSA
RLTWATHHVPFLAAVEDGPLSATQFHPEKSGDAGAALLRNWVEEL
>Mature_204_residues
SRKVVILDYGSGNLRSAQRAVERTGADVEVTADADAALNADGLVVPGVGAFEACMAGLREIGGEKVIAERVAAGRPVLGV
CVGMQILFARGVEFGVETAGCGQWPGSVVRLDAPVIPHMGWNVVDAPAGSTLFRGIDPDTRFYFVHSYAAQQWEGDPSAR
LTWATHHVPFLAAVEDGPLSATQFHPEKSGDAGAALLRNWVEEL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=210, Percent_Identity=40.9523809523809, Blast_Score=134, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=35.5140186915888, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21683; Mature: 21551

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKVVILDYGSGNLRSAQRAVERTGADVEVTADADAALNADGLVVPGVGAFEACMAGLR
CCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHHHH
EIGGEKVIAERVAAGRPVLGVCVGMQILFARGVEFGVETAGCGQWPGSVVRLDAPVIPHM
HCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEEEECCCCCCCC
GWNVVDAPAGSTLFRGIDPDTRFYFVHSYAAQQWEGDPSARLTWATHHVPFLAAVEDGPL
CCCEEECCCCCCEECCCCCCCEEEEEEECHHHHCCCCCCCEEEEEECCCEEEEEECCCCC
SATQFHPEKSGDAGAALLRNWVEEL
CCEEECCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
SRKVVILDYGSGNLRSAQRAVERTGADVEVTADADAALNADGLVVPGVGAFEACMAGLR
CCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHHHH
EIGGEKVIAERVAAGRPVLGVCVGMQILFARGVEFGVETAGCGQWPGSVVRLDAPVIPHM
HCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEEEECCCCCCCC
GWNVVDAPAGSTLFRGIDPDTRFYFVHSYAAQQWEGDPSARLTWATHHVPFLAAVEDGPL
CCCEEECCCCCCEECCCCCCCEEEEEEECHHHHCCCCCCCEEEEEECCCEEEEEECCCCC
SATQFHPEKSGDAGAALLRNWVEEL
CCEEECCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]