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The map label for this gene is recF [H]

Identifier: 108796985

GI number: 108796985

Start: 4241

End: 5383

Strand: Direct

Name: recF [H]

Synonym: Mmcs_0004

Alternate gene names: 108796985

Gene position: 4241-5383 (Clockwise)

Preceding gene: 108796984

Following gene: 108796986

Centisome position: 0.07

GC content: 69.9

Gene sequence:

>1143_bases
TTGTTCGTCCGGCATCTGACGCTGACCGACTTCCGGTCCTGGGCGCGCGCCGATCTGGAGTTGGAGCCCGGCCGTACCGT
CTTCGTCGGCCCCAACGGTTTCGGCAAGACGAATCTCGTTGAGGCGCTGTGGTATTCGGCAACGCTCGGCTCACACCGAG
TGGCCTCGGACGCACCGCTCATCCGGGTCGGCGCACCGCGCGCGGTGGTGTCGACCATCGTGGTGAACGAAGGTCGCGAG
TTGGCGGTCGACCTCGAGATCACCACCGGCCGGGCGAACAAGGCGCGGCTCAACCGTTCACCGGTGCGCAGCCCCCGGGA
AGTGCTCGGGGTGCTGCGTGCGGTGCTGTTCGCCCCCGAGGATCTGGCGCTGGTGCGCGGCGACCCGGGCGAACGCCGCC
GCTATCTCGACGAACTCGCCACCACCCGTCGCCCGAGCATCGCCGGCGTACGCGCCGACTACGACCGGGTCATCCGGCAG
CGCACCGCACTGCTGAAGAGCGCCGCGGGGGCCCGGTACCGGGGAGACCGCAGCGTTCTCGAGACCCTCGACGTGTGGGA
TGGGCACCTCGCCGCGCACGGTGCGTTGTTGATGGCCGCGCGCGCCGACCTCGTGCACCACTTGGCGCCGGAGGTCGAGA
AGGCCTATCAACTCCTGGCGCCGGGGTCGCGGCCGGCGGCCATCCGGTACCGCACCAGCATCGATGCCGAAGACGATGTC
AGCGCCGAGTACTACGAGGCCGCCCTGCTCGACGCGATGACCCGCCGGCGCGACGCCGAACTCGAACGCGGGGTCTGCCT
GGTCGGCCCGCACCGGGACGACCTGGAGTTGCGGCTCGGTGACCAGATGGCGAAAGGCTATGCCAGCCATGGGGAGTCGT
GGTCGATGGCGTTGTCGCTGCGGTTGGCCGCCTATGAGCTGTTGCGAACCGACGGCAGCGATCCGGTCCTCCTGCTCGAT
GACGTGTTCGCCGAACTGGACGCCGCCCGGCGGCGCGCCCTGGCCGAGGTCGCAGCGTCCGCGGAGCAGGTGCTGGTCAC
CGCGGCGGTCGCCGAGGACATCCCCGCCGACTGGGATGCGCGGCGGATCATGATCCGGATGCAGGACGACGACGATGGGC
GCGTATCGATGGTGGAGTCATGA

Upstream 100 bases:

>100_bases
AACCAGTTCGGCGGGCACGCCGTCCAGAGGATCAGCAAGTCGGGGTAGATGCCGCGGTTGCGAGGTGCAATGAGATGCCG
CGGACGCGAGGAGCAATGAG

Downstream 100 bases:

>100_bases
GCGACGACGACCGGGATGGCGACGGCGACCCGATCGGTCCCCCGGCGCACCTGTCCGAGCTCAAGGGAATGGATCTGGTG
CGGCGCACCCTCGAAGAAGC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MFVRHLTLTDFRSWARADLELEPGRTVFVGPNGFGKTNLVEALWYSATLGSHRVASDAPLIRVGAPRAVVSTIVVNEGRE
LAVDLEITTGRANKARLNRSPVRSPREVLGVLRAVLFAPEDLALVRGDPGERRRYLDELATTRRPSIAGVRADYDRVIRQ
RTALLKSAAGARYRGDRSVLETLDVWDGHLAAHGALLMAARADLVHHLAPEVEKAYQLLAPGSRPAAIRYRTSIDAEDDV
SAEYYEAALLDAMTRRRDAELERGVCLVGPHRDDLELRLGDQMAKGYASHGESWSMALSLRLAAYELLRTDGSDPVLLLD
DVFAELDAARRRALAEVAASAEQVLVTAAVAEDIPADWDARRIMIRMQDDDDGRVSMVES

Sequences:

>Translated_380_residues
MFVRHLTLTDFRSWARADLELEPGRTVFVGPNGFGKTNLVEALWYSATLGSHRVASDAPLIRVGAPRAVVSTIVVNEGRE
LAVDLEITTGRANKARLNRSPVRSPREVLGVLRAVLFAPEDLALVRGDPGERRRYLDELATTRRPSIAGVRADYDRVIRQ
RTALLKSAAGARYRGDRSVLETLDVWDGHLAAHGALLMAARADLVHHLAPEVEKAYQLLAPGSRPAAIRYRTSIDAEDDV
SAEYYEAALLDAMTRRRDAELERGVCLVGPHRDDLELRLGDQMAKGYASHGESWSMALSLRLAAYELLRTDGSDPVLLLD
DVFAELDAARRRALAEVAASAEQVLVTAAVAEDIPADWDARRIMIRMQDDDDGRVSMVES
>Mature_380_residues
MFVRHLTLTDFRSWARADLELEPGRTVFVGPNGFGKTNLVEALWYSATLGSHRVASDAPLIRVGAPRAVVSTIVVNEGRE
LAVDLEITTGRANKARLNRSPVRSPREVLGVLRAVLFAPEDLALVRGDPGERRRYLDELATTRRPSIAGVRADYDRVIRQ
RTALLKSAAGARYRGDRSVLETLDVWDGHLAAHGALLMAARADLVHHLAPEVEKAYQLLAPGSRPAAIRYRTSIDAEDDV
SAEYYEAALLDAMTRRRDAELERGVCLVGPHRDDLELRLGDQMAKGYASHGESWSMALSLRLAAYELLRTDGSDPVLLLD
DVFAELDAARRRALAEVAASAEQVLVTAAVAEDIPADWDARRIMIRMQDDDDGRVSMVES

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=361, Percent_Identity=27.9778393351801, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41880; Mature: 41880

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVRHLTLTDFRSWARADLELEPGRTVFVGPNGFGKTNLVEALWYSATLGSHRVASDAPL
CEEEEEEHHHHHHHHHCCCEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCE
IRVGAPRAVVSTIVVNEGRELAVDLEITTGRANKARLNRSPVRSPREVLGVLRAVLFAPE
EEECCCHHHHHHHHCCCCCEEEEEEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCC
DLALVRGDPGERRRYLDELATTRRPSIAGVRADYDRVIRQRTALLKSAAGARYRGDRSVL
CEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ETLDVWDGHLAAHGALLMAARADLVHHLAPEVEKAYQLLAPGSRPAAIRYRTSIDAEDDV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
SAEYYEAALLDAMTRRRDAELERGVCLVGPHRDDLELRLGDQMAKGYASHGESWSMALSL
CHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEECHHHHHHHHHCCCCEEHHHHH
RLAAYELLRTDGSDPVLLLDDVFAELDAARRRALAEVAASAEQVLVTAAVAEDIPADWDA
HHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RRIMIRMQDDDDGRVSMVES
EEEEEEEECCCCCCEEEECC
>Mature Secondary Structure
MFVRHLTLTDFRSWARADLELEPGRTVFVGPNGFGKTNLVEALWYSATLGSHRVASDAPL
CEEEEEEHHHHHHHHHCCCEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCE
IRVGAPRAVVSTIVVNEGRELAVDLEITTGRANKARLNRSPVRSPREVLGVLRAVLFAPE
EEECCCHHHHHHHHCCCCCEEEEEEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCC
DLALVRGDPGERRRYLDELATTRRPSIAGVRADYDRVIRQRTALLKSAAGARYRGDRSVL
CEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ETLDVWDGHLAAHGALLMAARADLVHHLAPEVEKAYQLLAPGSRPAAIRYRTSIDAEDDV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
SAEYYEAALLDAMTRRRDAELERGVCLVGPHRDDLELRLGDQMAKGYASHGESWSMALSL
CHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEECHHHHHHHHHCCCCEEHHHHH
RLAAYELLRTDGSDPVLLLDDVFAELDAARRRALAEVAASAEQVLVTAAVAEDIPADWDA
HHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RRIMIRMQDDDDGRVSMVES
EEEEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA