Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 108796983

GI number: 108796983

Start: 2069

End: 3265

Strand: Direct

Name: dnaN [H]

Synonym: Mmcs_0002

Alternate gene names: 108796983

Gene position: 2069-3265 (Clockwise)

Preceding gene: 108796982

Following gene: 108796984

Centisome position: 0.04

GC content: 70.34

Gene sequence:

>1197_bases
ATGGACGTGGTGCCGACGACGGCGGGTCACACCGACCTGAAGTTCCGCCTGGTGCGAGAGGACTTCGCCGACGCAGTGGC
CTGGGTGGCCCGCAATCTGCCGACCCGTCCGACCGTCCCCGTGCTGGCCGGCGTGCTGCTCACCGGCGACGACGACGGGC
TGACGATCTCCGGATTCGACTACGAGGTTTCGGCCGAGGTGCGTGTCGTGGCCGAAATCGCTTCTCCGGGAAGCGTTCTG
GTGTCGGGACGGCTGTTGTCCGATATCACCAAGGCGCTGCCCGCCAAACCCGTCGACGTCAGCGTCGAGGGCACCCGCGT
CTCGCTGACCTGCGGGAGCGCCCGCTTCTCGCTGCCGACCATGGCCGTCGAGGACTACCCCGCGCTGCCGACGCTGCCGG
ACGAGACCGGGGTGATCGGCGCCGACCTGTTCGCCGAAGCCATCGGCCAGGTCGCGGTGGCCGCCGGCCGCGACGACACG
CTGCCGATGCTCACCGGCATCCGCGTCGAGATCACAGGCGAGAGTGTGGTTTTGGCGGCAACCGACCGGTTCCGCCTGGC
GGTGCGCGAGTTGACCTGGTCGACGAGCTCGCCCGACGTCGAGGCCGCGGTGCTGGTACCGGCCAAGACCCTCGCCGAGG
CGGCCAAGGCCGGCACCGACGGGACCGATGTGCACCTGGCGCTGGGCGCCGGATCCGCGGTCGGCAAAGAGGGCCTGCTG
GGGATTCGCAGCGAGGGCAAGCGCAGCACCACCCGCCTGCTCGACGCGGAATTCCCGAAGTTCCGCCAACTGCTGCCCAG
TGAGCACACCGCGGTCGCGACGATCGGCGTCGCCGAACTCACCGAGGCGATCAAGCGTGTCGCGCTGGTGGCCGACCGCG
GCGCCCAGGTGCGGATGGAATTCGCCGACGACGTGTTGCGGTTGTCCGCCGGCGCCGACGACGTCGGACGCGCCGAGGAG
GACCTGGCCGTCGCGTTCTCCGGTGAGCCGCTCACCATCGCCTTCAACCCGACATATCTCACCGACGGCCTCGGCTCGCT
GCATTCCGAGCGGGTGACGTTCGGCTTCACGACGCCCAGCCGGCCCGCGGTCCTCCGCCCGGCGGGCGACGAGGACACCG
GCGGTGGCGCCGGACCGTTCCCGGCCCTGCAGACCGATTACGTTTACCTGTTGATGCCGGTACGGCTTCCCGGCTGA

Upstream 100 bases:

>100_bases
ACAGCCGTTACGGTCGTCGAGACGCATCAGGGCGACGCGTTCCGAGACGCCGGTGAAACCGCTGGTAGAGCTCATTGTGG
TGTTATTGCGAAGGGACGCT

Downstream 100 bases:

>100_bases
CCGGACCGACGCAACCAAGAGGGGCGGACATGCAACTGGGTCTGGTGGGGCTCGGCAAGATGGGCTTCAACATGCGGGAA
CGCCTTCGCGAAGGCGGACA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MDVVPTTAGHTDLKFRLVREDFADAVAWVARNLPTRPTVPVLAGVLLTGDDDGLTISGFDYEVSAEVRVVAEIASPGSVL
VSGRLLSDITKALPAKPVDVSVEGTRVSLTCGSARFSLPTMAVEDYPALPTLPDETGVIGADLFAEAIGQVAVAAGRDDT
LPMLTGIRVEITGESVVLAATDRFRLAVRELTWSTSSPDVEAAVLVPAKTLAEAAKAGTDGTDVHLALGAGSAVGKEGLL
GIRSEGKRSTTRLLDAEFPKFRQLLPSEHTAVATIGVAELTEAIKRVALVADRGAQVRMEFADDVLRLSAGADDVGRAEE
DLAVAFSGEPLTIAFNPTYLTDGLGSLHSERVTFGFTTPSRPAVLRPAGDEDTGGGAGPFPALQTDYVYLLMPVRLPG

Sequences:

>Translated_398_residues
MDVVPTTAGHTDLKFRLVREDFADAVAWVARNLPTRPTVPVLAGVLLTGDDDGLTISGFDYEVSAEVRVVAEIASPGSVL
VSGRLLSDITKALPAKPVDVSVEGTRVSLTCGSARFSLPTMAVEDYPALPTLPDETGVIGADLFAEAIGQVAVAAGRDDT
LPMLTGIRVEITGESVVLAATDRFRLAVRELTWSTSSPDVEAAVLVPAKTLAEAAKAGTDGTDVHLALGAGSAVGKEGLL
GIRSEGKRSTTRLLDAEFPKFRQLLPSEHTAVATIGVAELTEAIKRVALVADRGAQVRMEFADDVLRLSAGADDVGRAEE
DLAVAFSGEPLTIAFNPTYLTDGLGSLHSERVTFGFTTPSRPAVLRPAGDEDTGGGAGPFPALQTDYVYLLMPVRLPG
>Mature_398_residues
MDVVPTTAGHTDLKFRLVREDFADAVAWVARNLPTRPTVPVLAGVLLTGDDDGLTISGFDYEVSAEVRVVAEIASPGSVL
VSGRLLSDITKALPAKPVDVSVEGTRVSLTCGSARFSLPTMAVEDYPALPTLPDETGVIGADLFAEAIGQVAVAAGRDDT
LPMLTGIRVEITGESVVLAATDRFRLAVRELTWSTSSPDVEAAVLVPAKTLAEAAKAGTDGTDVHLALGAGSAVGKEGLL
GIRSEGKRSTTRLLDAEFPKFRQLLPSEHTAVATIGVAELTEAIKRVALVADRGAQVRMEFADDVLRLSAGADDVGRAEE
DLAVAFSGEPLTIAFNPTYLTDGLGSLHSERVTFGFTTPSRPAVLRPAGDEDTGGGAGPFPALQTDYVYLLMPVRLPG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=397, Percent_Identity=28.7153652392947, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41587; Mature: 41587

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVVPTTAGHTDLKFRLVREDFADAVAWVARNLPTRPTVPVLAGVLLTGDDDGLTISGFD
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCCCCEEEECCC
YEVSAEVRVVAEIASPGSVLVSGRLLSDITKALPAKPVDVSVEGTRVSLTCGSARFSLPT
EEECCCEEEEEEECCCCCEEEECHHHHHHHHHCCCCCCEEEECCCEEEEEECCCEECCCE
MAVEDYPALPTLPDETGVIGADLFAEAIGQVAVAAGRDDTLPMLTGIRVEITGESVVLAA
EEECCCCCCCCCCCCCCEEHHHHHHHHHHHEEEECCCCCCCCEEECEEEEECCCEEEEEE
TDRFRLAVRELTWSTSSPDVEAAVLVPAKTLAEAAKAGTDGTDVHLALGAGSAVGKEGLL
CCHHEEEEEHHCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCE
GIRSEGKRSTTRLLDAEFPKFRQLLPSEHTAVATIGVAELTEAIKRVALVADRGAQVRME
EECCCCCHHHHHHHCCCCHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEH
FADDVLRLSAGADDVGRAEEDLAVAFSGEPLTIAFNPTYLTDGLGSLHSERVTFGFTTPS
HHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEEEECCCEECCCCCCCCCCCEEEEECCCC
RPAVLRPAGDEDTGGGAGPFPALQTDYVYLLMPVRLPG
CCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MDVVPTTAGHTDLKFRLVREDFADAVAWVARNLPTRPTVPVLAGVLLTGDDDGLTISGFD
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCCCCEEEECCC
YEVSAEVRVVAEIASPGSVLVSGRLLSDITKALPAKPVDVSVEGTRVSLTCGSARFSLPT
EEECCCEEEEEEECCCCCEEEECHHHHHHHHHCCCCCCEEEECCCEEEEEECCCEECCCE
MAVEDYPALPTLPDETGVIGADLFAEAIGQVAVAAGRDDTLPMLTGIRVEITGESVVLAA
EEECCCCCCCCCCCCCCEEHHHHHHHHHHHEEEECCCCCCCCEEECEEEEECCCEEEEEE
TDRFRLAVRELTWSTSSPDVEAAVLVPAKTLAEAAKAGTDGTDVHLALGAGSAVGKEGLL
CCHHEEEEEHHCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCE
GIRSEGKRSTTRLLDAEFPKFRQLLPSEHTAVATIGVAELTEAIKRVALVADRGAQVRME
EECCCCCHHHHHHHCCCCHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEH
FADDVLRLSAGADDVGRAEEDLAVAFSGEPLTIAFNPTYLTDGLGSLHSERVTFGFTTPS
HHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEEEECCCEECCCCCCCCCCCEEEEECCCC
RPAVLRPAGDEDTGGGAGPFPALQTDYVYLLMPVRLPG
CCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8733228 [H]