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The map label for this gene is fhlA [H]

Identifier: 108763477

GI number: 108763477

Start: 1837171

End: 1838766

Strand: Direct

Name: fhlA [H]

Synonym: MXAN_1565

Alternate gene names: 108763477

Gene position: 1837171-1838766 (Clockwise)

Preceding gene: 108762522

Following gene: 108761569

Centisome position: 20.1

GC content: 70.49

Gene sequence:

>1596_bases
ATGCAGGACGACTTTTCAGGCGCCATCGACGGTGCGAAGGACGCTCGGGACCTCCTCGAGCTGGCAACGGCGGAGGATTC
CGTCGGGGATTTGCTGCGCCGTGGGCTCGACTGGCTGACGCGGGTGGTGCGCTTCGACCTGGCCACGGTGTTCCTCCTGA
AGGAGGGCCGGCTGGTGGCGGTGGCCGCACGAGGGTCGTTGGCCAACGCGAAGGTGCGGCAGCACTCGTTGCCGTTGTCG
GAGTTCCCCTCGCTGCGGCAGGCCCTGGAGACGCGGCGGGCGAAGGCCTTCACGGAAGAGGACCATGCCCACGGGGATGG
AGACCCTTTCGACGGCGTGTTGGATTTGCCGCCGGGGCATTCGTGCATGGTGGTGCCGCTGTGCGCGGGTGATCGCTGCT
ACGGCGTGCTCTCCCTGGACCGCGCCGAGTGTGAGACGTACCCCCAGCCGGTGGTGGACCTGGTGGAGGTGTACGGGCAG
ATGCTGGCCACGGCCATCCAGTCGGCCGAGCAGCGCGCCACCTTCGAGCGTCTCCACCGTCAGGACCACGAGCACGCGAA
GCTGCTGGAGGCGCAGCTCGGCGGAGACTCGGAGGGCATCCTCGAGACGTCGAAGAGCCCGGTGATGCGAGACCTGGCGC
GGCGGGCGCGGCAGGTGGCGGAGACGGACACGCCAGTGCTGATTACCGGTGAGACGGGCACGGGCAAGGAGCGGCTGGCC
CGCGCCATCCACCGTTGGAGCGCCCGCGCGGACCAGCCCTTCGTCACGCTCAACTGCGCTGCCATTCCGGCGGGCTTGCT
GGAGAGCGAGCTGTTTGGCCACGTGAAGGGCGCCTTCACCGGCGCCACGAAGGACCGGGCCGGACGCTTCCAGATGGCGC
ATGGCGGCACGCTGCTGCTGGATGAAGTGGGCGAGCTGCCCTTCGACCTCCAGGCGAAGCTGCTGCGGGCGCTGCAGGAG
AAGACATTCGAGCCGGTGGGCAGCGACAAGACGGTGCGCGCGGACGTGCGCATCCTCGCGGCCACGCACGTGGACCTTCA
GCAAGCCATTGCCCAGAAGCGCTTCCGCGAGGACCTCTACTACCGGTTGAGCGTGTTCCCCCTGCGCCTGCCGCCGCTGC
GCGAGCGCCGGGAGGACCTGCCGCAGTTGTGCGCCTTCCTCCTGGAGGAACAGGCGAACCGGACGGGACGGCGGGGCATG
CGGGTGACGCCAGAGGGCCTGGTGCGGTTGGAGGCCTACGAGTGGCCGGGCAACCTGCGCGAGCTGGCCAATGCGCTGGA
GCGGGCCACCATCCTCACGCGAGGGACGGAGCTGGGGCCGGAGTCCTTCGACGTTCCCACGCGCGGCGCGGCCGTGGTGG
CGGCCCTGCCGGAGGAGCCGGCGCCCGCGGCGGGAGGGCCGAAGCGGGGCCCCGTGCTGACGCTGGCGGCCGTGCAGCGC
GAGCACATCATGCGAGTGCTCACGCTCACGCGGGGCCGCGTCTACGGCGCCAATGGGGCCGCGGCGCTGCTGGGGCTCAA
GCCGTCCACGCTCCAGAGCCGGATGAAGAAGCTGGGCATCGCCCGGCTGGAGCAGTTCGTTGTCGACGAGGCGTGA

Upstream 100 bases:

>100_bases
GACGGATCTTCCGGCCTGGAGTGGTTGCCTGGACGGTGGGGCACGAAATCTCGGTGCCTGCCTCACTGCAATTTCGGTGG
AACCCTATTATTTCGTAGGT

Downstream 100 bases:

>100_bases
GCCTTGAAACCGCGCGCCTAGGAGACGCGCGGTGCCGAGGGCGCCACCGGGTAGGTTCCCCGCTGACCGAACGGCGGGTC
GAACAGCGCCGGGTCCGACT

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MQDDFSGAIDGAKDARDLLELATAEDSVGDLLRRGLDWLTRVVRFDLATVFLLKEGRLVAVAARGSLANAKVRQHSLPLS
EFPSLRQALETRRAKAFTEEDHAHGDGDPFDGVLDLPPGHSCMVVPLCAGDRCYGVLSLDRAECETYPQPVVDLVEVYGQ
MLATAIQSAEQRATFERLHRQDHEHAKLLEAQLGGDSEGILETSKSPVMRDLARRARQVAETDTPVLITGETGTGKERLA
RAIHRWSARADQPFVTLNCAAIPAGLLESELFGHVKGAFTGATKDRAGRFQMAHGGTLLLDEVGELPFDLQAKLLRALQE
KTFEPVGSDKTVRADVRILAATHVDLQQAIAQKRFREDLYYRLSVFPLRLPPLRERREDLPQLCAFLLEEQANRTGRRGM
RVTPEGLVRLEAYEWPGNLRELANALERATILTRGTELGPESFDVPTRGAAVVAALPEEPAPAAGGPKRGPVLTLAAVQR
EHIMRVLTLTRGRVYGANGAAALLGLKPSTLQSRMKKLGIARLEQFVVDEA

Sequences:

>Translated_531_residues
MQDDFSGAIDGAKDARDLLELATAEDSVGDLLRRGLDWLTRVVRFDLATVFLLKEGRLVAVAARGSLANAKVRQHSLPLS
EFPSLRQALETRRAKAFTEEDHAHGDGDPFDGVLDLPPGHSCMVVPLCAGDRCYGVLSLDRAECETYPQPVVDLVEVYGQ
MLATAIQSAEQRATFERLHRQDHEHAKLLEAQLGGDSEGILETSKSPVMRDLARRARQVAETDTPVLITGETGTGKERLA
RAIHRWSARADQPFVTLNCAAIPAGLLESELFGHVKGAFTGATKDRAGRFQMAHGGTLLLDEVGELPFDLQAKLLRALQE
KTFEPVGSDKTVRADVRILAATHVDLQQAIAQKRFREDLYYRLSVFPLRLPPLRERREDLPQLCAFLLEEQANRTGRRGM
RVTPEGLVRLEAYEWPGNLRELANALERATILTRGTELGPESFDVPTRGAAVVAALPEEPAPAAGGPKRGPVLTLAAVQR
EHIMRVLTLTRGRVYGANGAAALLGLKPSTLQSRMKKLGIARLEQFVVDEA
>Mature_531_residues
MQDDFSGAIDGAKDARDLLELATAEDSVGDLLRRGLDWLTRVVRFDLATVFLLKEGRLVAVAARGSLANAKVRQHSLPLS
EFPSLRQALETRRAKAFTEEDHAHGDGDPFDGVLDLPPGHSCMVVPLCAGDRCYGVLSLDRAECETYPQPVVDLVEVYGQ
MLATAIQSAEQRATFERLHRQDHEHAKLLEAQLGGDSEGILETSKSPVMRDLARRARQVAETDTPVLITGETGTGKERLA
RAIHRWSARADQPFVTLNCAAIPAGLLESELFGHVKGAFTGATKDRAGRFQMAHGGTLLLDEVGELPFDLQAKLLRALQE
KTFEPVGSDKTVRADVRILAATHVDLQQAIAQKRFREDLYYRLSVFPLRLPPLRERREDLPQLCAFLLEEQANRTGRRGM
RVTPEGLVRLEAYEWPGNLRELANALERATILTRGTELGPESFDVPTRGAAVVAALPEEPAPAAGGPKRGPVLTLAAVQR
EHIMRVLTLTRGRVYGANGAAALLGLKPSTLQSRMKKLGIARLEQFVVDEA

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=429, Percent_Identity=43.3566433566434, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI87082117, Length=445, Percent_Identity=40.8988764044944, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1788550, Length=323, Percent_Identity=43.6532507739938, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87082152, Length=425, Percent_Identity=39.5294117647059, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1790437, Length=350, Percent_Identity=41.7142857142857, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788905, Length=231, Percent_Identity=50.2164502164502, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1789233, Length=221, Percent_Identity=49.7737556561086, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1790299, Length=269, Percent_Identity=45.724907063197, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=46.7213114754098, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1787583, Length=250, Percent_Identity=42.8, Blast_Score=198, Evalue=9e-52,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=38.3647798742138, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=40.4858299595142, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI87081858, Length=335, Percent_Identity=31.044776119403, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58234; Mature: 58234

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDDFSGAIDGAKDARDLLELATAEDSVGDLLRRGLDWLTRVVRFDLATVFLLKEGRLVA
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEE
VAARGSLANAKVRQHSLPLSEFPSLRQALETRRAKAFTEEDHAHGDGDPFDGVLDLPPGH
EEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCC
SCMVVPLCAGDRCYGVLSLDRAECETYPQPVVDLVEVYGQMLATAIQSAEQRATFERLHR
CEEEEEEECCCCEEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDHEHAKLLEAQLGGDSEGILETSKSPVMRDLARRARQVAETDTPVLITGETGTGKERLA
CCHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHH
RAIHRWSARADQPFVTLNCAAIPAGLLESELFGHVKGAFTGATKDRAGRFQMAHGGTLLL
HHHHHHHCCCCCCEEEEEHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEE
DEVGELPFDLQAKLLRALQEKTFEPVGSDKTVRADVRILAATHVDLQQAIAQKRFREDLY
HHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHEEEEEHHHHHHHHHHHHHHHHHHHH
YRLSVFPLRLPPLRERREDLPQLCAFLLEEQANRTGRRGMRVTPEGLVRLEAYEWPGNLR
HHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCHHH
ELANALERATILTRGTELGPESFDVPTRGAAVVAALPEEPAPAAGGPKRGPVLTLAAVQR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEHHHHHH
EHIMRVLTLTRGRVYGANGAAALLGLKPSTLQSRMKKLGIARLEQFVVDEA
HHHHHHHHHHCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQDDFSGAIDGAKDARDLLELATAEDSVGDLLRRGLDWLTRVVRFDLATVFLLKEGRLVA
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEE
VAARGSLANAKVRQHSLPLSEFPSLRQALETRRAKAFTEEDHAHGDGDPFDGVLDLPPGH
EEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCC
SCMVVPLCAGDRCYGVLSLDRAECETYPQPVVDLVEVYGQMLATAIQSAEQRATFERLHR
CEEEEEEECCCCEEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDHEHAKLLEAQLGGDSEGILETSKSPVMRDLARRARQVAETDTPVLITGETGTGKERLA
CCHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHH
RAIHRWSARADQPFVTLNCAAIPAGLLESELFGHVKGAFTGATKDRAGRFQMAHGGTLLL
HHHHHHHCCCCCCEEEEEHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEE
DEVGELPFDLQAKLLRALQEKTFEPVGSDKTVRADVRILAATHVDLQQAIAQKRFREDLY
HHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHEEEEEHHHHHHHHHHHHHHHHHHHH
YRLSVFPLRLPPLRERREDLPQLCAFLLEEQANRTGRRGMRVTPEGLVRLEAYEWPGNLR
HHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCHHH
ELANALERATILTRGTELGPESFDVPTRGAAVVAALPEEPAPAAGGPKRGPVLTLAAVQR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEHHHHHH
EHIMRVLTLTRGRVYGANGAAALLGLKPSTLQSRMKKLGIARLEQFVVDEA
HHHHHHHHHHCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]