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The map label for this gene is slt [H]

Identifier: 108762740

GI number: 108762740

Start: 255625

End: 258021

Strand: Direct

Name: slt [H]

Synonym: MXAN_0210

Alternate gene names: 108762740

Gene position: 255625-258021 (Clockwise)

Preceding gene: 108760488

Following gene: 108759272

Centisome position: 2.8

GC content: 72.8

Gene sequence:

>2397_bases
ATGGACGGACTTCGAGCAGGTGCCGTGGTGTTCCTGGCGTGCGCGGGCATGCTGGGAGCAGGTCCTTCCTGGGCGCAGGC
GCCCCTGGAGGAAGAGCCCGGCGAGGTGCTTTCCGAGGACCAGTTGGAAGCACTGCTCGACTCACCGGTGGCGCAGCCTT
CCGACGGTGAGCTGTCCACCGCGGCCTTCGGACCGGAGCGGTTGGCGCCGTACTTCGTGGAAGGCACCCTGGCAAAGGCC
CACGCGGAGTTCCGGCGCGGGCGCTACGCGCGGGCGCGGAGCCTGCTGGCCCATGAGGAGGCCACGCCGCAGGTTCGCTT
CCTCAGGGCGCAGTGCGCGCTGCTGGCGCGCAACCACGCGGTGGCGGCCGAGGAGTTCGCCGCGCTGGCGGACGGCTACG
GCGCGCTGCGCGACCACAGCCTGCTGCGGGCCGCCCAGGCCAACGAGCGGCTGCGCAAGCTGGACACGGCCGCCGGGCAG
TACCGCGGCGTGAGCCCGGGCTCGCCGCTGTACCCGGAGGCGCGCTTCAGCCTGTCGCGGGTGCTCCAGCGGCGGGGGGA
CATTCCGGGCGCGTTGACGGCGCTCCAGGAGCTCATCGACAGCCGCCAGTCGCGAGGGCCGGACGCGCTCCGGATGAAGG
CGCTGCTGGCCATCTGCGACCTGGCGCGCGCGCAAGGGCAGTACAACGCCGAGCACCGCGCGCTGCTGGAGGTGTGGGCC
ACCAGTCCGCTGTCGAACGAGGCGCGCCGCGCCGAGAAGCGGTTGAAGGGGTTGCCGCTGCCCCTCAAGTGGCGGGTGCG
CCGCGCGGAGGCGCTGGTGGAGCTGCACCGCAACCATGCCGGCATGGCGCTGCTGGACAAGGTGCTGCCGCACCTGGAGA
TGCCGGACGAGCTGGCCTGCCGCGCGCACCTCACCTACGGCCGGGCGCTGCGCAAGGAGCGCAAGCACCGCCGGGCCATC
GAGGCGCTGGAGCCCATGGTGGTGGGCTGCACCGCGTCCGAGCAGCGGCCGCAGGCGCTCTACATCCTGGGCTACTCCCA
GTCGGTGGTGGAGCCGAAGGCGGCCGTCACCACCTATGCCATGCTCGCCCGGGACTACCCGGAGCATGGCTACGCGGATG
ACGCGCTCTTCTTCGAGGCGTGGTTGCTCCAGCGCACGGGCGACGTGGAGACGGCCCTGGCGCGCTACGAAGAGGCCGCG
CGGCGCTACCCGGCGGGGAACTTCGCGTCGGAGTCGCTCTTCCGTGCCTTCTGGCTGCACTCCCGCAAGGCCAACGCCCC
GGCGGCCCTGGCCGCGCTGAAGGCGGTGGAGAACCTGCCCGAGGCGGCACGCACGGACGAGGCGCTGTGGCGCTCGCGGT
ACTGGCGTGCGCGCATGCAGGAGTCCGGCGCCTCGGCGCAGGCGGCGCTCGATGCGTACGCGCACATCGCCGCCGAGCGC
CCCGCGGCCTGGTACGGGATGCTGGCCCGCTCACGGCTCGCGCTGCTCTCACCGGAGCGCGTGGCGCGGCTGTACCCCAA
GCCCGTGCCTCCCGAGGCCATGGAGGGCACCGGCGGCTCCGGCCGCGATGACGCCCCGGCGGACGAAGTGGTGGAGGAGG
TGGGCGAGCCCGAGGAGATCTGGCCGCTGCCGCCCGGCGCGCTGGCGAAGGACGCGCGCTTCAACGCGGGCGTGGAGCTG
ATGCGCCTGGGGCTGCCCGGCGCGGTGGATGAGTTCCTCGCCGTGGATGCGCGCGCGCTGCCCGAGGCCCCGGCGCGGCT
GCTGTATCAGACGCTGAAGCGCACCGGCCGCAATCGGGCCGCGCGGCAGGTGGCGCGCACCTCGCTGAAGCAGGAGGTGC
ACGGTCCGCTGAGCGCCGCGTCCCGTCCCATCTGGGAGGCCACCTGGCCGCTGGCGTACCGCTCGTCCATTGCCCGTTAC
GCGCGGGCCCATCGCGTGGACCCCGACCTGCTCCAGGGCCTCATCCGCGAGGAGAGCCGCTTCAATGCCCGCGCGCGTTC
GGCCACGGGCGCGCTGGGCCTGGCGCAGTTGATGCCCACCACGGCGAGACAGGTGGCGGACTCACTGGACATGCCGATGC
CGGGTGAGTCCGCGTTGCTCCAGCCTCACACGAACATCCGCCTGGGTGCGGCGTACCTGGGACAACTGGTCAAACACTTC
GGAGGCAACGTGGCCTACGCGGTGGCGGCCTACAACGCCGGGCCGCGCGCGGTGGAGCGCTGGCGGCATGCGCTGCCCGA
AGCGGAGCTCGACGAGTGGGTGGAGCACATCGGCTTCGAGGAGACGCGCGAGTACGTGAAGAAGGTGCTCGGCAGCTACA
GCGCCTACAAGCTGCTCTACGCGGACGAGCCCGCGCTGCTGAGGGACCTGCGCCTCAGTGACGCCAGCCGGCGGTGA

Upstream 100 bases:

>100_bases
GCTGGATGACGGGCGCGCGGAAAGTCATGTGAGGTACCCGTCCCAATCGCACTCCGAGTGCCGATGCATTGCCGCCCCTG
GATTCAAGACGAGGGCAGTC

Downstream 100 bases:

>100_bases
AGCGCGGCGTCCCAGCGGAACCTCCAGGCGACACGGTGCCGTGCCGCCCGGAGGAGGGGACTCAGAAGTTGAGGCCGCCG
TCCACGTCGATGGTGCGGCC

Product: transglycosylase SLT domain-containing protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 798; Mature: 798

Protein sequence:

>798_residues
MDGLRAGAVVFLACAGMLGAGPSWAQAPLEEEPGEVLSEDQLEALLDSPVAQPSDGELSTAAFGPERLAPYFVEGTLAKA
HAEFRRGRYARARSLLAHEEATPQVRFLRAQCALLARNHAVAAEEFAALADGYGALRDHSLLRAAQANERLRKLDTAAGQ
YRGVSPGSPLYPEARFSLSRVLQRRGDIPGALTALQELIDSRQSRGPDALRMKALLAICDLARAQGQYNAEHRALLEVWA
TSPLSNEARRAEKRLKGLPLPLKWRVRRAEALVELHRNHAGMALLDKVLPHLEMPDELACRAHLTYGRALRKERKHRRAI
EALEPMVVGCTASEQRPQALYILGYSQSVVEPKAAVTTYAMLARDYPEHGYADDALFFEAWLLQRTGDVETALARYEEAA
RRYPAGNFASESLFRAFWLHSRKANAPAALAALKAVENLPEAARTDEALWRSRYWRARMQESGASAQAALDAYAHIAAER
PAAWYGMLARSRLALLSPERVARLYPKPVPPEAMEGTGGSGRDDAPADEVVEEVGEPEEIWPLPPGALAKDARFNAGVEL
MRLGLPGAVDEFLAVDARALPEAPARLLYQTLKRTGRNRAARQVARTSLKQEVHGPLSAASRPIWEATWPLAYRSSIARY
ARAHRVDPDLLQGLIREESRFNARARSATGALGLAQLMPTTARQVADSLDMPMPGESALLQPHTNIRLGAAYLGQLVKHF
GGNVAYAVAAYNAGPRAVERWRHALPEAELDEWVEHIGFEETREYVKKVLGSYSAYKLLYADEPALLRDLRLSDASRR

Sequences:

>Translated_798_residues
MDGLRAGAVVFLACAGMLGAGPSWAQAPLEEEPGEVLSEDQLEALLDSPVAQPSDGELSTAAFGPERLAPYFVEGTLAKA
HAEFRRGRYARARSLLAHEEATPQVRFLRAQCALLARNHAVAAEEFAALADGYGALRDHSLLRAAQANERLRKLDTAAGQ
YRGVSPGSPLYPEARFSLSRVLQRRGDIPGALTALQELIDSRQSRGPDALRMKALLAICDLARAQGQYNAEHRALLEVWA
TSPLSNEARRAEKRLKGLPLPLKWRVRRAEALVELHRNHAGMALLDKVLPHLEMPDELACRAHLTYGRALRKERKHRRAI
EALEPMVVGCTASEQRPQALYILGYSQSVVEPKAAVTTYAMLARDYPEHGYADDALFFEAWLLQRTGDVETALARYEEAA
RRYPAGNFASESLFRAFWLHSRKANAPAALAALKAVENLPEAARTDEALWRSRYWRARMQESGASAQAALDAYAHIAAER
PAAWYGMLARSRLALLSPERVARLYPKPVPPEAMEGTGGSGRDDAPADEVVEEVGEPEEIWPLPPGALAKDARFNAGVEL
MRLGLPGAVDEFLAVDARALPEAPARLLYQTLKRTGRNRAARQVARTSLKQEVHGPLSAASRPIWEATWPLAYRSSIARY
ARAHRVDPDLLQGLIREESRFNARARSATGALGLAQLMPTTARQVADSLDMPMPGESALLQPHTNIRLGAAYLGQLVKHF
GGNVAYAVAAYNAGPRAVERWRHALPEAELDEWVEHIGFEETREYVKKVLGSYSAYKLLYADEPALLRDLRLSDASRR
>Mature_798_residues
MDGLRAGAVVFLACAGMLGAGPSWAQAPLEEEPGEVLSEDQLEALLDSPVAQPSDGELSTAAFGPERLAPYFVEGTLAKA
HAEFRRGRYARARSLLAHEEATPQVRFLRAQCALLARNHAVAAEEFAALADGYGALRDHSLLRAAQANERLRKLDTAAGQ
YRGVSPGSPLYPEARFSLSRVLQRRGDIPGALTALQELIDSRQSRGPDALRMKALLAICDLARAQGQYNAEHRALLEVWA
TSPLSNEARRAEKRLKGLPLPLKWRVRRAEALVELHRNHAGMALLDKVLPHLEMPDELACRAHLTYGRALRKERKHRRAI
EALEPMVVGCTASEQRPQALYILGYSQSVVEPKAAVTTYAMLARDYPEHGYADDALFFEAWLLQRTGDVETALARYEEAA
RRYPAGNFASESLFRAFWLHSRKANAPAALAALKAVENLPEAARTDEALWRSRYWRARMQESGASAQAALDAYAHIAAER
PAAWYGMLARSRLALLSPERVARLYPKPVPPEAMEGTGGSGRDDAPADEVVEEVGEPEEIWPLPPGALAKDARFNAGVEL
MRLGLPGAVDEFLAVDARALPEAPARLLYQTLKRTGRNRAARQVARTSLKQEVHGPLSAASRPIWEATWPLAYRSSIARY
ARAHRVDPDLLQGLIREESRFNARARSATGALGLAQLMPTTARQVADSLDMPMPGESALLQPHTNIRLGAAYLGQLVKHF
GGNVAYAVAAYNAGPRAVERWRHALPEAELDEWVEHIGFEETREYVKKVLGSYSAYKLLYADEPALLRDLRLSDASRR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=221, Percent_Identity=32.1266968325792, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 87892; Mature: 87892

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGLRAGAVVFLACAGMLGAGPSWAQAPLEEEPGEVLSEDQLEALLDSPVAQPSDGELST
CCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCH
AAFGPERLAPYFVEGTLAKAHAEFRRGRYARARSLLAHEEATPQVRFLRAQCALLARNHA
HCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VAAEEFAALADGYGALRDHSLLRAAQANERLRKLDTAAGQYRGVSPGSPLYPEARFSLSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
VLQRRGDIPGALTALQELIDSRQSRGPDALRMKALLAICDLARAQGQYNAEHRALLEVWA
HHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TSPLSNEARRAEKRLKGLPLPLKWRVRRAEALVELHRNHAGMALLDKVLPHLEMPDELAC
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
RAHLTYGRALRKERKHRRAIEALEPMVVGCTASEQRPQALYILGYSQSVVEPKAAVTTYA
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCHHHHCCHHHHHHHH
MLARDYPEHGYADDALFFEAWLLQRTGDVETALARYEEAARRYPAGNFASESLFRAFWLH
HHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SRKANAPAALAALKAVENLPEAARTDEALWRSRYWRARMQESGASAQAALDAYAHIAAER
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
PAAWYGMLARSRLALLSPERVARLYPKPVPPEAMEGTGGSGRDDAPADEVVEEVGEPEEI
CHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHCCCCCCCCCCCCCHHHHHHHCCCCCCC
WPLPPGALAKDARFNAGVELMRLGLPGAVDEFLAVDARALPEAPARLLYQTLKRTGRNRA
CCCCCCCHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHH
ARQVARTSLKQEVHGPLSAASRPIWEATWPLAYRSSIARYARAHRVDPDLLQGLIREESR
HHHHHHHHHHHHHCCCHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FNARARSATGALGLAQLMPTTARQVADSLDMPMPGESALLQPHTNIRLGAAYLGQLVKHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCEECHHHHHHHHHHH
GGNVAYAVAAYNAGPRAVERWRHALPEAELDEWVEHIGFEETREYVKKVLGSYSAYKLLY
CCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHEEEEE
ADEPALLRDLRLSDASRR
CCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDGLRAGAVVFLACAGMLGAGPSWAQAPLEEEPGEVLSEDQLEALLDSPVAQPSDGELST
CCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCH
AAFGPERLAPYFVEGTLAKAHAEFRRGRYARARSLLAHEEATPQVRFLRAQCALLARNHA
HCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VAAEEFAALADGYGALRDHSLLRAAQANERLRKLDTAAGQYRGVSPGSPLYPEARFSLSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
VLQRRGDIPGALTALQELIDSRQSRGPDALRMKALLAICDLARAQGQYNAEHRALLEVWA
HHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TSPLSNEARRAEKRLKGLPLPLKWRVRRAEALVELHRNHAGMALLDKVLPHLEMPDELAC
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
RAHLTYGRALRKERKHRRAIEALEPMVVGCTASEQRPQALYILGYSQSVVEPKAAVTTYA
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCHHHHCCHHHHHHHH
MLARDYPEHGYADDALFFEAWLLQRTGDVETALARYEEAARRYPAGNFASESLFRAFWLH
HHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SRKANAPAALAALKAVENLPEAARTDEALWRSRYWRARMQESGASAQAALDAYAHIAAER
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
PAAWYGMLARSRLALLSPERVARLYPKPVPPEAMEGTGGSGRDDAPADEVVEEVGEPEEI
CHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHCCCCCCCCCCCCCHHHHHHHCCCCCCC
WPLPPGALAKDARFNAGVELMRLGLPGAVDEFLAVDARALPEAPARLLYQTLKRTGRNRA
CCCCCCCHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHH
ARQVARTSLKQEVHGPLSAASRPIWEATWPLAYRSSIARYARAHRVDPDLLQGLIREESR
HHHHHHHHHHHHHCCCHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FNARARSATGALGLAQLMPTTARQVADSLDMPMPGESALLQPHTNIRLGAAYLGQLVKHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCEECHHHHHHHHHHH
GGNVAYAVAAYNAGPRAVERWRHALPEAELDEWVEHIGFEETREYVKKVLGSYSAYKLLY
CCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHEEEEE
ADEPALLRDLRLSDASRR
CCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]