| Definition | Myxococcus xanthus DK 1622 chromosome, complete genome. |
|---|---|
| Accession | NC_008095 |
| Length | 9,139,763 |
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The map label for this gene is gra-orf6 [H]
Identifier: 108761429
GI number: 108761429
Start: 1782959
End: 1783738
Strand: Direct
Name: gra-orf6 [H]
Synonym: MXAN_1520
Alternate gene names: 108761429
Gene position: 1782959-1783738 (Clockwise)
Preceding gene: 108760581
Following gene: 108763429
Centisome position: 19.51
GC content: 71.79
Gene sequence:
>780_bases GTGGACCTGGAACTGGGTGGCAAGGTGGTACTGGTGACGGGTGGCTCGGAGGGGCTGGGCGCCGCGGTGGCCCGGCGGCT CGTCCGGGAGGGCGCGAAGGTGGCGCTCTGTGCCCGTGGGGCGGAGCGGCTGGAAGCCACCGCCGCGGCCCTGCGCGCCG AGGGTGGGGACGTGCTCACCGTCCAGGCCGACGTGTCGCGTGCGTGGGAGGTGGAGCACTTCGTGGATGCCGCCCACGCG CGCTTCGGGCGCATCGACGGGCTGGTGAACAACGCGGGCACCGCGGCGGGCCGGCCCTTCGCCTCGGTGAGTGACGCGGA GTGGGAGGCCGACCTCCAGCTCAAGGTCTTCGCGGCGATTCGTGCCTCGCGGCAGGCGCTGCCCTTCCTGAAGGAGTCGG GGAGCGGCGCCATCGTCAACGTGCTGGCCATCGCGGCGAAGACGCCCGGCGCAAACTCCACGCCGTCGTCGGTGTCTCGC GCGGCGGGGCTCGCCTTGACGAAGGCGCTGTCCAAGGAGTTGGGCCCGCACGCCATCCGGGTGAACGCGGTGCTGGTGGG CATCATCGAAAGTGGCCAGTGGGCGCGCCGTGCCAAGGAGGTGGGTAAGCCCATGGAGTCCTTCCTGCAGGAGATGGCGC GTCACGCCGGGATTCCGCTGGGCCGGGTGGGGAAGGCGGAGGAGTTCGCCGATACCGTGGCCTTCCTGCTGTCGCCCCGT GGTGGCTACATCAGCGGCGCGGCCATCAATGTGGATGGCGGCCTGTCCGCGGCCGTCTGA
Upstream 100 bases:
>100_bases TGGTGAAGCAGCCCGCTTGATGACGTGACGTCCGGCGTCCCGGCCCCGTGCAAGGTGCAGTCGCCTCGCGGGGCCGGCGC TGGCCGCGAGGAGGTGGCGC
Downstream 100 bases:
>100_bases TTTCCCTTCGTCAGGAACGGGCCGGGTGCCCGTGTGTGGATGATGAAGACGGCTTCGCGCCGTCTGGCGACCGCGGCTGC TGACCCCTGGTCCCTCATCT
Product: short chain dehydrogenase/reductase family oxidoreductase
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: ORF6 [H]
Number of amino acids: Translated: 259; Mature: 259
Protein sequence:
>259_residues MDLELGGKVVLVTGGSEGLGAAVARRLVREGAKVALCARGAERLEATAAALRAEGGDVLTVQADVSRAWEVEHFVDAAHA RFGRIDGLVNNAGTAAGRPFASVSDAEWEADLQLKVFAAIRASRQALPFLKESGSGAIVNVLAIAAKTPGANSTPSSVSR AAGLALTKALSKELGPHAIRVNAVLVGIIESGQWARRAKEVGKPMESFLQEMARHAGIPLGRVGKAEEFADTVAFLLSPR GGYISGAAINVDGGLSAAV
Sequences:
>Translated_259_residues MDLELGGKVVLVTGGSEGLGAAVARRLVREGAKVALCARGAERLEATAAALRAEGGDVLTVQADVSRAWEVEHFVDAAHA RFGRIDGLVNNAGTAAGRPFASVSDAEWEADLQLKVFAAIRASRQALPFLKESGSGAIVNVLAIAAKTPGANSTPSSVSR AAGLALTKALSKELGPHAIRVNAVLVGIIESGQWARRAKEVGKPMESFLQEMARHAGIPLGRVGKAEEFADTVAFLLSPR GGYISGAAINVDGGLSAAV >Mature_259_residues MDLELGGKVVLVTGGSEGLGAAVARRLVREGAKVALCARGAERLEATAAALRAEGGDVLTVQADVSRAWEVEHFVDAAHA RFGRIDGLVNNAGTAAGRPFASVSDAEWEADLQLKVFAAIRASRQALPFLKESGSGAIVNVLAIAAKTPGANSTPSSVSR AAGLALTKALSKELGPHAIRVNAVLVGIIESGQWARRAKEVGKPMESFLQEMARHAGIPLGRVGKAEEFADTVAFLLSPR GGYISGAAINVDGGLSAAV
Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=254, Percent_Identity=34.6456692913386, Blast_Score=125, Evalue=4e-29, Organism=Homo sapiens, GI19923817, Length=258, Percent_Identity=32.1705426356589, Blast_Score=118, Evalue=4e-27, Organism=Homo sapiens, GI5031737, Length=251, Percent_Identity=34.2629482071713, Blast_Score=116, Evalue=3e-26, Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=31.6, Blast_Score=106, Evalue=2e-23, Organism=Homo sapiens, GI15277342, Length=256, Percent_Identity=32.421875, Blast_Score=104, Evalue=9e-23, Organism=Homo sapiens, GI7705925, Length=263, Percent_Identity=31.9391634980989, Blast_Score=95, Evalue=5e-20, Organism=Homo sapiens, GI126723191, Length=184, Percent_Identity=34.7826086956522, Blast_Score=94, Evalue=9e-20, Organism=Homo sapiens, GI33667109, Length=191, Percent_Identity=35.0785340314136, Blast_Score=94, Evalue=1e-19, Organism=Homo sapiens, GI10190704, Length=249, Percent_Identity=31.3253012048193, Blast_Score=94, Evalue=2e-19, Organism=Homo sapiens, GI126723750, Length=255, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=7e-19, Organism=Homo sapiens, GI59889578, Length=232, Percent_Identity=28.8793103448276, Blast_Score=89, Evalue=4e-18, Organism=Homo sapiens, GI66933014, Length=253, Percent_Identity=27.6679841897233, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI31542939, Length=227, Percent_Identity=29.5154185022026, Blast_Score=83, Evalue=2e-16, Organism=Homo sapiens, GI4503301, Length=254, Percent_Identity=30.3149606299213, Blast_Score=80, Evalue=1e-15, Organism=Homo sapiens, GI109715829, Length=241, Percent_Identity=29.045643153527, Blast_Score=79, Evalue=6e-15, Organism=Homo sapiens, GI4503817, Length=212, Percent_Identity=27.8301886792453, Blast_Score=77, Evalue=1e-14, Organism=Homo sapiens, GI224922801, Length=172, Percent_Identity=30.8139534883721, Blast_Score=71, Evalue=1e-12, Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=28.8537549407115, Blast_Score=106, Evalue=2e-24, Organism=Escherichia coli, GI1787335, Length=255, Percent_Identity=28.6274509803922, Blast_Score=102, Evalue=2e-23, Organism=Escherichia coli, GI87082160, Length=256, Percent_Identity=29.296875, Blast_Score=100, Evalue=1e-22, Organism=Escherichia coli, GI1790717, Length=260, Percent_Identity=27.3076923076923, Blast_Score=100, Evalue=2e-22, Organism=Escherichia coli, GI87082100, Length=255, Percent_Identity=30.9803921568627, Blast_Score=99, Evalue=4e-22, Organism=Escherichia coli, GI1788459, Length=253, Percent_Identity=29.2490118577075, Blast_Score=88, Evalue=5e-19, Organism=Escherichia coli, GI1789208, Length=255, Percent_Identity=27.0588235294118, Blast_Score=85, Evalue=5e-18, Organism=Escherichia coli, GI1786812, Length=256, Percent_Identity=29.296875, Blast_Score=80, Evalue=2e-16, Organism=Escherichia coli, GI2367175, Length=260, Percent_Identity=31.9230769230769, Blast_Score=79, Evalue=2e-16, Organism=Escherichia coli, GI1787820, Length=183, Percent_Identity=31.1475409836066, Blast_Score=79, Evalue=3e-16, Organism=Escherichia coli, GI2367365, Length=255, Percent_Identity=28.2352941176471, Blast_Score=75, Evalue=4e-15, Organism=Escherichia coli, GI1789057, Length=256, Percent_Identity=24.609375, Blast_Score=74, Evalue=7e-15, Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=26.8774703557312, Blast_Score=72, Evalue=5e-14, Organism=Caenorhabditis elegans, GI17560676, Length=256, Percent_Identity=31.640625, Blast_Score=123, Evalue=1e-28, Organism=Caenorhabditis elegans, GI17560332, Length=259, Percent_Identity=34.3629343629344, Blast_Score=115, Evalue=2e-26, Organism=Caenorhabditis elegans, GI17562910, Length=260, Percent_Identity=32.3076923076923, Blast_Score=113, Evalue=8e-26, Organism=Caenorhabditis elegans, GI17559104, Length=265, Percent_Identity=33.9622641509434, Blast_Score=113, Evalue=1e-25, Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=32.4, Blast_Score=113, Evalue=1e-25, Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=31.0756972111554, Blast_Score=111, Evalue=3e-25, Organism=Caenorhabditis elegans, GI17562904, Length=268, Percent_Identity=32.4626865671642, Blast_Score=109, Evalue=2e-24, Organism=Caenorhabditis elegans, GI17551412, Length=273, Percent_Identity=32.2344322344322, Blast_Score=108, Evalue=3e-24, Organism=Caenorhabditis elegans, GI17544670, Length=267, Percent_Identity=30.3370786516854, Blast_Score=105, Evalue=2e-23, Organism=Caenorhabditis elegans, GI17562906, Length=268, Percent_Identity=31.7164179104478, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=30.7086614173228, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI17562990, Length=264, Percent_Identity=31.4393939393939, Blast_Score=101, Evalue=4e-22, Organism=Caenorhabditis elegans, GI72000259, Length=268, Percent_Identity=32.8358208955224, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17562908, Length=265, Percent_Identity=32.0754716981132, Blast_Score=98, Evalue=4e-21, Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=30.5970149253731, Blast_Score=98, Evalue=4e-21, Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=31.0861423220974, Blast_Score=98, Evalue=5e-21, Organism=Caenorhabditis elegans, GI17560150, Length=259, Percent_Identity=30.8880308880309, Blast_Score=97, Evalue=6e-21, Organism=Caenorhabditis elegans, GI17538480, Length=273, Percent_Identity=30.7692307692308, Blast_Score=97, Evalue=7e-21, Organism=Caenorhabditis elegans, GI17565030, Length=260, Percent_Identity=31.9230769230769, Blast_Score=97, Evalue=1e-20, Organism=Caenorhabditis elegans, GI25147288, Length=262, Percent_Identity=31.2977099236641, Blast_Score=96, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17563726, Length=263, Percent_Identity=31.9391634980989, Blast_Score=94, Evalue=6e-20, Organism=Caenorhabditis elegans, GI17536025, Length=263, Percent_Identity=33.0798479087452, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17536651, Length=255, Percent_Identity=31.7647058823529, Blast_Score=92, Evalue=3e-19, Organism=Caenorhabditis elegans, GI115534694, Length=260, Percent_Identity=28.8461538461538, Blast_Score=90, Evalue=1e-18, Organism=Caenorhabditis elegans, GI17538486, Length=262, Percent_Identity=29.3893129770992, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17560220, Length=265, Percent_Identity=30.188679245283, Blast_Score=86, Evalue=3e-17, Organism=Caenorhabditis elegans, GI17555706, Length=252, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=2e-16, Organism=Caenorhabditis elegans, GI17508651, Length=252, Percent_Identity=28.968253968254, Blast_Score=78, Evalue=4e-15, Organism=Caenorhabditis elegans, GI17561272, Length=265, Percent_Identity=30.9433962264151, Blast_Score=77, Evalue=1e-14, Organism=Caenorhabditis elegans, GI17538182, Length=262, Percent_Identity=26.3358778625954, Blast_Score=67, Evalue=6e-12, Organism=Caenorhabditis elegans, GI193203117, Length=172, Percent_Identity=26.7441860465116, Blast_Score=67, Evalue=1e-11, Organism=Caenorhabditis elegans, GI193203115, Length=168, Percent_Identity=26.7857142857143, Blast_Score=65, Evalue=4e-11, Organism=Caenorhabditis elegans, GI17568967, Length=194, Percent_Identity=24.7422680412371, Blast_Score=64, Evalue=5e-11, Organism=Caenorhabditis elegans, GI17564282, Length=191, Percent_Identity=25.130890052356, Blast_Score=64, Evalue=1e-10, Organism=Saccharomyces cerevisiae, GI6324126, Length=253, Percent_Identity=28.8537549407115, Blast_Score=84, Evalue=2e-17, Organism=Saccharomyces cerevisiae, GI6323882, Length=193, Percent_Identity=31.0880829015544, Blast_Score=77, Evalue=2e-15, Organism=Saccharomyces cerevisiae, GI6322067, Length=185, Percent_Identity=31.8918918918919, Blast_Score=69, Evalue=7e-13, Organism=Saccharomyces cerevisiae, GI6322861, Length=192, Percent_Identity=24.4791666666667, Blast_Score=64, Evalue=2e-11, Organism=Drosophila melanogaster, GI23397609, Length=253, Percent_Identity=36.7588932806324, Blast_Score=132, Evalue=2e-31, Organism=Drosophila melanogaster, GI21355319, Length=255, Percent_Identity=32.156862745098, Blast_Score=122, Evalue=3e-28, Organism=Drosophila melanogaster, GI24643142, Length=262, Percent_Identity=32.824427480916, Blast_Score=118, Evalue=3e-27, Organism=Drosophila melanogaster, GI28571526, Length=250, Percent_Identity=33.6, Blast_Score=110, Evalue=1e-24, Organism=Drosophila melanogaster, GI24639444, Length=260, Percent_Identity=33.8461538461538, Blast_Score=110, Evalue=1e-24, Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=32.6771653543307, Blast_Score=101, Evalue=4e-22, Organism=Drosophila melanogaster, GI24644339, Length=258, Percent_Identity=29.0697674418605, Blast_Score=91, Evalue=9e-19, Organism=Drosophila melanogaster, GI24644337, Length=255, Percent_Identity=31.3725490196078, Blast_Score=79, Evalue=3e-15, Organism=Drosophila melanogaster, GI21358495, Length=195, Percent_Identity=29.2307692307692, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI281364892, Length=195, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI24580925, Length=179, Percent_Identity=32.9608938547486, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI17737361, Length=262, Percent_Identity=30.1526717557252, Blast_Score=68, Evalue=6e-12, Organism=Drosophila melanogaster, GI24584752, Length=173, Percent_Identity=32.9479768786127, Blast_Score=67, Evalue=9e-12, Organism=Drosophila melanogaster, GI116007236, Length=243, Percent_Identity=25.5144032921811, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI116007238, Length=184, Percent_Identity=29.3478260869565, Blast_Score=66, Evalue=3e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 26597; Mature: 26597
Theoretical pI: Translated: 9.20; Mature: 9.20
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.5 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDLELGGKVVLVTGGSEGLGAAVARRLVREGAKVALCARGAERLEATAAALRAEGGDVLT CCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEHHCHHHHHHHHHHHHCCCCCEEE VQADVSRAWEVEHFVDAAHARFGRIDGLVNNAGTAAGRPFASVSDAEWEADLQLKVFAAI EEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH RASRQALPFLKESGSGAIVNVLAIAAKTPGANSTPSSVSRAAGLALTKALSKELGPHAIR HHHHHHCCHHHCCCCCCEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE VNAVLVGIIESGQWARRAKEVGKPMESFLQEMARHAGIPLGRVGKAEEFADTVAFLLSPR EEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC GGYISGAAINVDGGLSAAV CCEEECEEEEECCCCCCCC >Mature Secondary Structure MDLELGGKVVLVTGGSEGLGAAVARRLVREGAKVALCARGAERLEATAAALRAEGGDVLT CCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEHHCHHHHHHHHHHHHCCCCCEEE VQADVSRAWEVEHFVDAAHARFGRIDGLVNNAGTAAGRPFASVSDAEWEADLQLKVFAAI EEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH RASRQALPFLKESGSGAIVNVLAIAAKTPGANSTPSSVSRAAGLALTKALSKELGPHAIR HHHHHHCCHHHCCCCCCEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE VNAVLVGIIESGQWARRAKEVGKPMESFLQEMARHAGIPLGRVGKAEEFADTVAFLLSPR EEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC GGYISGAAINVDGGLSAAV CCEEECEEEEECCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 2583128; 9831526 [H]