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The map label for this gene is glnG [H]

Identifier: 108760318

GI number: 108760318

Start: 523979

End: 524746

Strand: Direct

Name: glnG [H]

Synonym: MXAN_0460

Alternate gene names: 108760318

Gene position: 523979-524746 (Clockwise)

Preceding gene: 108758078

Following gene: 108758648

Centisome position: 5.73

GC content: 69.66

Gene sequence:

>768_bases
ATGATGGAGGACGTCGCAGTGGCCCCCGCGCGCATCCTGCTGGTCGACGACGAGGAGTCGCTTCGCATCACCTTGGCCGC
GAACCTGGAGCTGGAGGGCCACACGGTCCTCGAGGCGGCCAATGGTGAGGAGGCCTTGCGCCTCCTGGAGAAGCAATCCG
TGGACGTGGTGCTCACGGACATCCGCATGCCCGGCCTGCACGGCGTGGAGCTCTTGCGCCGCATCAAGCAGGAGCGCCCC
GACATGCCGGTGGTGCTGATGACGGCCTTCACGGCGGAGGAGCTGGTGGATGACGCGCTCGCCGAGGGCGCCTTCACCGT
GCTGCCCAAGCCCTTCGACGTGACGCACGCGCTGGACACGGTGCTGCGCGCGGCGGCGGCGCCGCAGGTCCTGGTGGTGG
ACGACACCGAACAGGTGGCGCGCGGCATGGTGCGCGCCCTGAGCACGGTGGGGCTGCGCGCGCGCGCCGTGTACAGCGGC
GAGGAGGCGTTGGCCTCGTTGCGTTCGGGGGAATACGACGTCTGCGTGCTGGACCTGGTGATGCCGGAGATGAGCGGGCC
GGAGCTGGTGTCCAAGGTGCGCGCGGCGAACCTGTCCGTCGCCATCATCGCCGTGTCTGGCCACGTGGTGCCGGAGATGC
TCCGCAGGGTGGCGGCGCAAGGCGCGGTGGTGTGCATGACCAAGCCGGTACCGTTGCGCGAGCTGGTGCAGGCGATTGCA
CGGGTGCGAGGCCGGCCTCAACAAGGGCTGCAAGGCGCGAGGATTTGA

Upstream 100 bases:

>100_bases
TGGTGCAACGTCATGGCGGCACAATCTCTGTGCGCAGCGAAGCGGAGCGGGGTAGTGAGTTCCTCATCGAGCTCCCCGCG
GCAGCGGCGGCCCAGGCCGC

Downstream 100 bases:

>100_bases
CCCATGGCAGGCAGAGACCTCCAGGCGCGTGAGCGGGCGGCGGTGGCGGACGTGGTGGCCTCCACCCTGCGGCACGACCT
GCGCAACAAGCTGGCCAGCA

Product: response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MMEDVAVAPARILLVDDEESLRITLAANLELEGHTVLEAANGEEALRLLEKQSVDVVLTDIRMPGLHGVELLRRIKQERP
DMPVVLMTAFTAEELVDDALAEGAFTVLPKPFDVTHALDTVLRAAAAPQVLVVDDTEQVARGMVRALSTVGLRARAVYSG
EEALASLRSGEYDVCVLDLVMPEMSGPELVSKVRAANLSVAIIAVSGHVVPEMLRRVAAQGAVVCMTKPVPLRELVQAIA
RVRGRPQQGLQGARI

Sequences:

>Translated_255_residues
MMEDVAVAPARILLVDDEESLRITLAANLELEGHTVLEAANGEEALRLLEKQSVDVVLTDIRMPGLHGVELLRRIKQERP
DMPVVLMTAFTAEELVDDALAEGAFTVLPKPFDVTHALDTVLRAAAAPQVLVVDDTEQVARGMVRALSTVGLRARAVYSG
EEALASLRSGEYDVCVLDLVMPEMSGPELVSKVRAANLSVAIIAVSGHVVPEMLRRVAAQGAVVCMTKPVPLRELVQAIA
RVRGRPQQGLQGARI
>Mature_255_residues
MMEDVAVAPARILLVDDEESLRITLAANLELEGHTVLEAANGEEALRLLEKQSVDVVLTDIRMPGLHGVELLRRIKQERP
DMPVVLMTAFTAEELVDDALAEGAFTVLPKPFDVTHALDTVLRAAAAPQVLVVDDTEQVARGMVRALSTVGLRARAVYSG
EEALASLRSGEYDVCVLDLVMPEMSGPELVSKVRAANLSVAIIAVSGHVVPEMLRRVAAQGAVVCMTKPVPLRELVQAIA
RVRGRPQQGLQGARI

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=118, Percent_Identity=42.3728813559322, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788550, Length=143, Percent_Identity=37.7622377622378, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788905, Length=124, Percent_Identity=37.9032258064516, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790437, Length=135, Percent_Identity=35.5555555555556, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1789809, Length=105, Percent_Identity=36.1904761904762, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788222, Length=133, Percent_Identity=33.0827067669173, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 27447; Mature: 27447

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEDVAVAPARILLVDDEESLRITLAANLELEGHTVLEAANGEEALRLLEKQSVDVVLTD
CCCCCEECCEEEEEEECCCCEEEEEEEEEEECCCEEEEECCHHHHHHHHHHCCCCEEEEE
IRMPGLHGVELLRRIKQERPDMPVVLMTAFTAEELVDDALAEGAFTVLPKPFDVTHALDT
CCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHH
VLRAAAAPQVLVVDDTEQVARGMVRALSTVGLRARAVYSGEEALASLRSGEYDVCVLDLV
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHCHHHEEEECCHHHHHHHHCCCCCEEEEEEC
MPEMSGPELVSKVRAANLSVAIIAVSGHVVPEMLRRVAAQGAVVCMTKPVPLRELVQAIA
CCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
RVRGRPQQGLQGARI
HHCCCCCCCCCCCCC
>Mature Secondary Structure
MMEDVAVAPARILLVDDEESLRITLAANLELEGHTVLEAANGEEALRLLEKQSVDVVLTD
CCCCCEECCEEEEEEECCCCEEEEEEEEEEECCCEEEEECCHHHHHHHHHHCCCCEEEEE
IRMPGLHGVELLRRIKQERPDMPVVLMTAFTAEELVDDALAEGAFTVLPKPFDVTHALDT
CCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHH
VLRAAAAPQVLVVDDTEQVARGMVRALSTVGLRARAVYSGEEALASLRSGEYDVCVLDLV
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHCHHHEEEECCHHHHHHHHCCCCCEEEEEEC
MPEMSGPELVSKVRAANLSVAIIAVSGHVVPEMLRRVAAQGAVVCMTKPVPLRELVQAIA
CCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
RVRGRPQQGLQGARI
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]