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The map label for this gene is zraS [H]

Identifier: 108758078

GI number: 108758078

Start: 522573

End: 523982

Strand: Direct

Name: zraS [H]

Synonym: MXAN_0459

Alternate gene names: 108758078

Gene position: 522573-523982 (Clockwise)

Preceding gene: 108759071

Following gene: 108760318

Centisome position: 5.72

GC content: 69.29

Gene sequence:

>1410_bases
ATGAGCCCAGCGCGCGACGAGCCGTCCAGCCCGCCCAGCCCGCCCATGGCGCTCAAGGAGCGCGCCGCGCGGCTGTTCCG
CGAGCACCTGTCCGCGGTGCGGGTCCGCACGGACCGGCTCTTCGCGGGGCTGATGCTGGGCCAGTGGGCGGTGGGCATCG
TCATCGCGCTGTTTGGTCCGCGGGGCAGCGGCGCGCAGCTCCAGGCGGCCGTGCTGGTGGGGACGCTGCTCAGCGCCTTC
CCGATGGTGATGGCCCGCTGGCGCCCGGGCTCCATGCCCACCCGTCACGTGGTGGCCGTATGCCAGGCGCTCTGGTCCTC
GCTGCTCATCTACCTGACGGACGGCCGGGTGGAGACGCACTTCCACATCTTCGGCTCGCTGGCCTTCCTGGCGCTGTACC
GCGACCCGTGGGTGCTGCTCTCCGCGAGCATCACCACCGTCGTGGACCACGTGATGCGCGGCACCCTGTGGTCACACTCG
CTCTACGGCGGGCTTCCGCCGGAGCTGTGGCGGTTCGTGGAGCACGCCTTCTGGGTGGCCTTCATCGACGTGGTGCTGGT
GTATTCGTGCCGGGGCATGTTGCGCGAGCTGCGCGAGGTGGCGGTGCGCCGGGCGGAGCTGGAGCTGGCCCGCGAACGGG
AGCACGCGAAGGCGCGCGAGCTGGACCGCGCCCTGCGCGAGCTCAGCGGCTTCCAGGAGCACCTCATCCGCGTGGAGAAG
CTGGCCGCGGTGGGACAGTTGGCCGCCAGCGTGGGGCACGAGCTGCGAAACCCGCTGGCCGCCGTGCGCAACTCCCACGC
CTATCTCTCCCGGAAGCTCACGAGGGACCCAGCAGGGGCCGCGGATGACCCACGGGTCCCCCAGTTCCTGGGGCTGATGG
AGCGCGAGCTGAACGCCTGCGCGAAAATCATCTCCGACCTGCTCGACTTCGCTCGCGAGCGTCCGCCCGCGCTCCAGCCG
TGTCCGCTGCGTCCGTTGGTGGACGAGGCCATTGGCGTGGTGCCGCAGCGGGAAGGGGTGCGAATCGTCAATGACGTGCC
GGAGTCGTTGCCGGTGCCGCACCTGGACAAGGAGCAGTTCCGTCAGGTTCTGGTGAACCTGGTGCAGAACGCAGTGGAGG
CCATGCCCGTGGGGCGCAATGGAGTGGTTTCAGTCGTAGCGGAAGGTGGAGACGCGAGTCCCTGGCGCATCCGGGTCATG
GATGACGGCGCGGGGATTCCTCCGGAGGTGTTGCCCAAGATTTTCGAACCGCTCTTCACCACGAAGACGCGTGGCACCGG
CTTGGGACTGGCCATTGTCGCCAACATGGTGCAACGTCATGGCGGCACAATCTCTGTGCGCAGCGAAGCGGAGCGGGGTA
GTGAGTTCCTCATCGAGCTCCCCGCGGCAGCGGCGGCCCAGGCCGCATGA

Upstream 100 bases:

>100_bases
GCGCCGCGGTGGAGGCGCTGCTGGGTGACGCGCCGGCGGAAGCGCTGGCCGGGCACGCCGTCTACGGCTGTGAGCTGGAA
GACCCCCGCGGGAGCGTGCC

Downstream 100 bases:

>100_bases
TGGAGGACGTCGCAGTGGCCCCCGCGCGCATCCTGCTGGTCGACGACGAGGAGTCGCTTCGCATCACCTTGGCCGCGAAC
CTGGAGCTGGAGGGCCACAC

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSPARDEPSSPPSPPMALKERAARLFREHLSAVRVRTDRLFAGLMLGQWAVGIVIALFGPRGSGAQLQAAVLVGTLLSAF
PMVMARWRPGSMPTRHVVAVCQALWSSLLIYLTDGRVETHFHIFGSLAFLALYRDPWVLLSASITTVVDHVMRGTLWSHS
LYGGLPPELWRFVEHAFWVAFIDVVLVYSCRGMLRELREVAVRRAELELAREREHAKARELDRALRELSGFQEHLIRVEK
LAAVGQLAASVGHELRNPLAAVRNSHAYLSRKLTRDPAGAADDPRVPQFLGLMERELNACAKIISDLLDFARERPPALQP
CPLRPLVDEAIGVVPQREGVRIVNDVPESLPVPHLDKEQFRQVLVNLVQNAVEAMPVGRNGVVSVVAEGGDASPWRIRVM
DDGAGIPPEVLPKIFEPLFTTKTRGTGLGLAIVANMVQRHGGTISVRSEAERGSEFLIELPAAAAAQAA

Sequences:

>Translated_469_residues
MSPARDEPSSPPSPPMALKERAARLFREHLSAVRVRTDRLFAGLMLGQWAVGIVIALFGPRGSGAQLQAAVLVGTLLSAF
PMVMARWRPGSMPTRHVVAVCQALWSSLLIYLTDGRVETHFHIFGSLAFLALYRDPWVLLSASITTVVDHVMRGTLWSHS
LYGGLPPELWRFVEHAFWVAFIDVVLVYSCRGMLRELREVAVRRAELELAREREHAKARELDRALRELSGFQEHLIRVEK
LAAVGQLAASVGHELRNPLAAVRNSHAYLSRKLTRDPAGAADDPRVPQFLGLMERELNACAKIISDLLDFARERPPALQP
CPLRPLVDEAIGVVPQREGVRIVNDVPESLPVPHLDKEQFRQVLVNLVQNAVEAMPVGRNGVVSVVAEGGDASPWRIRVM
DDGAGIPPEVLPKIFEPLFTTKTRGTGLGLAIVANMVQRHGGTISVRSEAERGSEFLIELPAAAAAQAA
>Mature_468_residues
SPARDEPSSPPSPPMALKERAARLFREHLSAVRVRTDRLFAGLMLGQWAVGIVIALFGPRGSGAQLQAAVLVGTLLSAFP
MVMARWRPGSMPTRHVVAVCQALWSSLLIYLTDGRVETHFHIFGSLAFLALYRDPWVLLSASITTVVDHVMRGTLWSHSL
YGGLPPELWRFVEHAFWVAFIDVVLVYSCRGMLRELREVAVRRAELELAREREHAKARELDRALRELSGFQEHLIRVEKL
AAVGQLAASVGHELRNPLAAVRNSHAYLSRKLTRDPAGAADDPRVPQFLGLMERELNACAKIISDLLDFARERPPALQPC
PLRPLVDEAIGVVPQREGVRIVNDVPESLPVPHLDKEQFRQVLVNLVQNAVEAMPVGRNGVVSVVAEGGDASPWRIRVMD
DGAGIPPEVLPKIFEPLFTTKTRGTGLGLAIVANMVQRHGGTISVRSEAERGSEFLIELPAAAAAQAA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=242, Percent_Identity=38.4297520661157, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788549, Length=269, Percent_Identity=33.0855018587361, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1789149, Length=286, Percent_Identity=28.3216783216783, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI145693157, Length=234, Percent_Identity=32.9059829059829, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081816, Length=278, Percent_Identity=28.7769784172662, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790300, Length=240, Percent_Identity=28.3333333333333, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994928, Length=262, Percent_Identity=27.4809160305344, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786912, Length=227, Percent_Identity=28.1938325991189, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788393, Length=229, Percent_Identity=28.3842794759825, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786783, Length=250, Percent_Identity=28, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786600, Length=237, Percent_Identity=27.0042194092827, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51526; Mature: 51395

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPARDEPSSPPSPPMALKERAARLFREHLSAVRVRTDRLFAGLMLGQWAVGIVIALFGP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RGSGAQLQAAVLVGTLLSAFPMVMARWRPGSMPTRHVVAVCQALWSSLLIYLTDGRVETH
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCEEHH
FHIFGSLAFLALYRDPWVLLSASITTVVDHVMRGTLWSHSLYGGLPPELWRFVEHAFWVA
HHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
FIDVVLVYSCRGMLRELREVAVRRAELELAREREHAKARELDRALRELSGFQEHLIRVEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAVGQLAASVGHELRNPLAAVRNSHAYLSRKLTRDPAGAADDPRVPQFLGLMERELNAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
AKIISDLLDFARERPPALQPCPLRPLVDEAIGVVPQREGVRIVNDVPESLPVPHLDKEQF
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHCCHHHCCCCCCCHHHH
RQVLVNLVQNAVEAMPVGRNGVVSVVAEGGDASPWRIRVMDDGAGIPPEVLPKIFEPLFT
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
TKTRGTGLGLAIVANMVQRHGGTISVRSEAERGSEFLIELPAAAAAQAA
CCCCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCHHEEECCCHHHCCCC
>Mature Secondary Structure 
SPARDEPSSPPSPPMALKERAARLFREHLSAVRVRTDRLFAGLMLGQWAVGIVIALFGP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RGSGAQLQAAVLVGTLLSAFPMVMARWRPGSMPTRHVVAVCQALWSSLLIYLTDGRVETH
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCEEHH
FHIFGSLAFLALYRDPWVLLSASITTVVDHVMRGTLWSHSLYGGLPPELWRFVEHAFWVA
HHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
FIDVVLVYSCRGMLRELREVAVRRAELELAREREHAKARELDRALRELSGFQEHLIRVEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAVGQLAASVGHELRNPLAAVRNSHAYLSRKLTRDPAGAADDPRVPQFLGLMERELNAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
AKIISDLLDFARERPPALQPCPLRPLVDEAIGVVPQREGVRIVNDVPESLPVPHLDKEQF
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHCCHHHCCCCCCCHHHH
RQVLVNLVQNAVEAMPVGRNGVVSVVAEGGDASPWRIRVMDDGAGIPPEVLPKIFEPLFT
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
TKTRGTGLGLAIVANMVQRHGGTISVRSEAERGSEFLIELPAAAAAQAA
CCCCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCHHEEECCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]