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The map label for this gene is proB

Identifier: 108758489

GI number: 108758489

Start: 1592712

End: 1593845

Strand: Direct

Name: proB

Synonym: MXAN_1357

Alternate gene names: 108758489

Gene position: 1592712-1593845 (Clockwise)

Preceding gene: 108758245

Following gene: 108763289

Centisome position: 17.43

GC content: 71.96

Gene sequence:

>1134_bases
GTGAGTTCGATTTCCACGGGACGCACCGCCCTACGCTCCGCCCGGCGGGTGGTGGTGAAAATCGGCACCAACGCGCTGAC
GAACGCCACCGGACGTTTCAACCGTCAGCACTTCGATGCGCTGGGCCAGGATTTGTTGTGGGCCGCGCAGGGCCGCGAGC
TGGTGGTGGTGTCCAGCGGGGCCATTGCCCTGGGGGTCGAGCGGCTGGGCCTGCCCTCGCGCCCTCGCGACATCCCGGGC
AAGCAGGCGTGCGCGGCGGTGGGGCAGAGCCGGCTGGTGCAGGCCTACGAAGAGGCCTTCGCCGCGCATGGGAAGGCGGT
GGCCCAGGTGCTGCTCACCCACGAGGACGTGCAGGAGCGCCGCCGCTACCTCAACGTGAAGCACACCCTGGAGCGGTTGC
TGACGGCGGGCGTCGTCCCCGTCATCAACGAGAACGACACCGTCTCCGTGGACGAGCTGAAGTTCGGTGACAACGACACG
CTGGCGGGGCTGGTGGCCGGCGTGGTGGATGCCGACGCGCTCGTCCTGCTGTCGGATGTGGAGGGCCTCTACACCGGAGA
CCCCCGGCGCGACGCAGGCGCGGAGCTGCTGGCCACGGTGATGCAGGTGACGCCCGAGGTCCTGGCGCTCGCCACCGGTA
CGAGCAGCGGGGTGGGGACGGGCGGGATGAGCACCAAGGTGCGGGCGGCCGCGCGCGCGTCCGATTCCGGCATTCACTGC
GTGATTACGTCCGGCGCCGTGCCCGGCCGCCTGCGCGCCGTCCTGGAAGGCGCGGACGTGGGGACCCACTTCGAACCCAC
CGGCAGCCGCCGCAGTGCCCGCGCCGCGTGGATTGCCCATGCCCTGCGCGCACGCGGGACGCTCACGGTGGATGCCGGCG
CGCGCGAGGCCATTGTCACCGGCAAGCGCAGCCTGCTGCCCAGCGGCGTTCGCGGCGTGGAGGGGGACTTCGGGCGCGGA
GACCCGGTGGACCTGGTGGACGCGGAGGGGGCCGTGTTCGCGCGGGGGCTGGCGGCCTACGACGCCAACGAGCTGCGACG
CATCGCCGGACATCGCACGGCGGACATCGAAGCGGTGCTGGGCTACCGCTACCTCGACGAGGCCGTGCACCGCGACGACC
TGGCCGTGCTGTAG

Upstream 100 bases:

>100_bases
ATTGGGGCCCCCACCTTTTTCGTACGACTTTATGACTGCCGCTGTGGGGAAAACACGCCGGACGCGCGCGTCTTCCATTT
CTGACCTACGCTGACGCCCT

Downstream 100 bases:

>100_bases
CCGGGCAGGGCGTTGCTCAGATGGACGAGGTGGACAGCGACTTGCCGCCGCTTTCGCCCAACTGCATGAGCGCGCGGAAC
TCCGGCGAGTCCACCTTCAT

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSSISTGRTALRSARRVVVKIGTNALTNATGRFNRQHFDALGQDLLWAAQGRELVVVSSGAIALGVERLGLPSRPRDIPG
KQACAAVGQSRLVQAYEEAFAAHGKAVAQVLLTHEDVQERRRYLNVKHTLERLLTAGVVPVINENDTVSVDELKFGDNDT
LAGLVAGVVDADALVLLSDVEGLYTGDPRRDAGAELLATVMQVTPEVLALATGTSSGVGTGGMSTKVRAAARASDSGIHC
VITSGAVPGRLRAVLEGADVGTHFEPTGSRRSARAAWIAHALRARGTLTVDAGAREAIVTGKRSLLPSGVRGVEGDFGRG
DPVDLVDAEGAVFARGLAAYDANELRRIAGHRTADIEAVLGYRYLDEAVHRDDLAVL

Sequences:

>Translated_377_residues
MSSISTGRTALRSARRVVVKIGTNALTNATGRFNRQHFDALGQDLLWAAQGRELVVVSSGAIALGVERLGLPSRPRDIPG
KQACAAVGQSRLVQAYEEAFAAHGKAVAQVLLTHEDVQERRRYLNVKHTLERLLTAGVVPVINENDTVSVDELKFGDNDT
LAGLVAGVVDADALVLLSDVEGLYTGDPRRDAGAELLATVMQVTPEVLALATGTSSGVGTGGMSTKVRAAARASDSGIHC
VITSGAVPGRLRAVLEGADVGTHFEPTGSRRSARAAWIAHALRARGTLTVDAGAREAIVTGKRSLLPSGVRGVEGDFGRG
DPVDLVDAEGAVFARGLAAYDANELRRIAGHRTADIEAVLGYRYLDEAVHRDDLAVL
>Mature_376_residues
SSISTGRTALRSARRVVVKIGTNALTNATGRFNRQHFDALGQDLLWAAQGRELVVVSSGAIALGVERLGLPSRPRDIPGK
QACAAVGQSRLVQAYEEAFAAHGKAVAQVLLTHEDVQERRRYLNVKHTLERLLTAGVVPVINENDTVSVDELKFGDNDTL
AGLVAGVVDADALVLLSDVEGLYTGDPRRDAGAELLATVMQVTPEVLALATGTSSGVGTGGMSTKVRAAARASDSGIHCV
ITSGAVPGRLRAVLEGADVGTHFEPTGSRRSARAAWIAHALRARGTLTVDAGAREAIVTGKRSLLPSGVRGVEGDFGRGD
PVDLVDAEGAVFARGLAAYDANELRRIAGHRTADIEAVLGYRYLDEAVHRDDLAVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=304, Percent_Identity=33.5526315789474, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI62912457, Length=302, Percent_Identity=33.4437086092715, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=45.4794520547945, Blast_Score=299, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17569969, Length=318, Percent_Identity=33.6477987421384, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17569967, Length=318, Percent_Identity=33.6477987421384, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=30.7506053268765, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321822, Length=420, Percent_Identity=29.5238095238095, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI21357643, Length=297, Percent_Identity=35.016835016835, Blast_Score=144, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_MYXXD (Q1DCK9)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_629614.1
- ProteinModelPortal:   Q1DCK9
- STRING:   Q1DCK9
- GeneID:   4103854
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_1357
- TIGR:   MXAN_1357
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q1DCK9
- ProtClustDB:   CLSK944899
- BioCyc:   MXAN246197:MXAN_1357-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39602; Mature: 39471

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSISTGRTALRSARRVVVKIGTNALTNATGRFNRQHFDALGQDLLWAAQGRELVVVSSG
CCCCCHHHHHHHHHHEEEEEECCCHHHCCCCCCCHHHHHHHHHHHEECCCCCEEEEEECC
AIALGVERLGLPSRPRDIPGKQACAAVGQSRLVQAYEEAFAAHGKAVAQVLLTHEDVQER
CCEECHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRYLNVKHTLERLLTAGVVPVINENDTVSVDELKFGDNDTLAGLVAGVVDADALVLLSDV
HHHCCHHHHHHHHHHCCCCEEECCCCCEEHHEEECCCCCHHHHHHHHHHCCCCEEEEECC
EGLYTGDPRRDAGAELLATVMQVTPEVLALATGTSSGVGTGGMSTKVRAAARASDSGIHC
CCCCCCCCCCCHHHHHHHHHHHHCHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCEEE
VITSGAVPGRLRAVLEGADVGTHFEPTGSRRSARAAWIAHALRARGTLTVDAGAREAIVT
EEECCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHHC
GKRSLLPSGVRGVEGDFGRGDPVDLVDAEGAVFARGLAAYDANELRRIAGHRTADIEAVL
CCHHHCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHH
GYRYLDEAVHRDDLAVL
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSISTGRTALRSARRVVVKIGTNALTNATGRFNRQHFDALGQDLLWAAQGRELVVVSSG
CCCCHHHHHHHHHHEEEEEECCCHHHCCCCCCCHHHHHHHHHHHEECCCCCEEEEEECC
AIALGVERLGLPSRPRDIPGKQACAAVGQSRLVQAYEEAFAAHGKAVAQVLLTHEDVQER
CCEECHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRYLNVKHTLERLLTAGVVPVINENDTVSVDELKFGDNDTLAGLVAGVVDADALVLLSDV
HHHCCHHHHHHHHHHCCCCEEECCCCCEEHHEEECCCCCHHHHHHHHHHCCCCEEEEECC
EGLYTGDPRRDAGAELLATVMQVTPEVLALATGTSSGVGTGGMSTKVRAAARASDSGIHC
CCCCCCCCCCCHHHHHHHHHHHHCHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCEEE
VITSGAVPGRLRAVLEGADVGTHFEPTGSRRSARAAWIAHALRARGTLTVDAGAREAIVT
EEECCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHHC
GKRSLLPSGVRGVEGDFGRGDPVDLVDAEGAVFARGLAAYDANELRRIAGHRTADIEAVL
CCHHHCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHH
GYRYLDEAVHRDDLAVL
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA