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The map label for this gene is glyS [H]

Identifier: 108563378

GI number: 108563378

Start: 998370

End: 1000475

Strand: Reverse

Name: glyS [H]

Synonym: HPAG1_0953

Alternate gene names: 108563378

Gene position: 1000475-998370 (Counterclockwise)

Preceding gene: 108563382

Following gene: 108563377

Centisome position: 62.67

GC content: 38.03

Gene sequence:

>2106_bases
TTGCATTCAGATGAATTGTTAGTAGAGATTTTGGTTGAAGAATTGCCCGCGCAAGCGTTATTGAATGAATATAAAGAAAT
GCCTAAAAAACTCCATGCTCTTCTTAATAAGCGTGCTTTAGAAGCGGGAAATATAGAGATTTTTTACACCCCTAGGCGTT
TGTGTTTGCTCATTAAAGATTTTCCTCTTTTAACCCAAGAAACTAAAGAGGAATTTTTTGGGCCTCCCATTAAAATCGCA
TTCAACAATGAAGATAAAACGCAAGGGTTGAACGCTCTAGGTTTAGGGTTTTATCAAAAATTAGGACTAAAGGATCACCA
GCATTTCCAAACAGCGTTCAAAAACAATAAAGAAGTGCTTTATCACGCTAAAATCCACGAAAAAGAGCCTACAAAAGACT
TAATCATGCCCATTGTGTTAGAGTTTTTAGAGGGTTTGAATTTCGGGAAGTCTATGCGTTGGGGCAATGTGGAAAAAAGC
TTTATCAGACCCATTCATAATATTTGCGTGTTGTTTAATGGGGAAAATTTTAACGGTATTGAAGTCAAAGAGTATGGCTT
TAAAACCAAGCAAGCCACCAAAGTGCACCGACAAGAGGGTTTTGATTTTATTGAAGTGGATAGCCCTAAAGCCTATTTTG
AAGTTTTAGAAAAAAACCATGTCATTTTAGACCCTAAAAAGCGCGAAGCTAAAATCTTACAAGAAATTAAAGAGCTAGAA
ACAAAGCATGACATTATCGTAGAAATAGATAGGGATCTATTAGATGAGGTTGTAGCGATCACGGAATACCCTAGCGCGCT
TTTAGGGGAGTTTGACAAGGCGTTTTTAAAATTACCTAATGAAATCATCACCACTTCCATGAAAGAAAACCAACGCTATT
TTGCGGTTTTTGATCAAAAAAGCCAAGAAGAGAGCCCAACATTACACAACGGCTTTATTGTGGTGAGCAACGCTATCAAT
AAAGACAAGCAAAAAATCATTGCAGGCAACCAAAAGGTTTTAAAAGCCCGTTTGAGCGATGCGGTTTTCTTTTATGAAAA
CGATCTCAAAAAGCCTTTAGATAACACCCCTTTAGAGAGCGTGGTTTTTGTGCAAGGTTTAGGGACTTTAAAGGATAAAA
TGGAGCGCGAAGCGATCATCGCCCAATACTTGACGCAAAAATACGCTCCATCTTTAAACATGCCTTTAGAAAAAGCCCTT
GAATTGATTGGCCGAGCTGTTAAAATCGCTAAGGCGGATTTACTCAGTGAAGTGGTGTATGAATTTAGCGAGCTTCAAGG
GATCATGGGCTATTACTACGCTTTAAAACAAAATGAAAACGAGTTGGTCGCCTTGAGTGTGAAAGAGCAGTATTTGCCCG
CAAGCGAAAACGCTCCCTTGCCCTCTAGCGTTTTTAGCGCGATCGTGGCTTTGAGCTTGAAATTAGACAGCCTGTTTTCT
CTTTTTAGCGCGGGTAAAATCCCTAGCGGATCTAAAGATCCTTTTGCTTTAAGGCGCTTGAGTTTTGGGCTATTGAAAAT
CGTCGCGCATTACGGGTTAGAATTTGATTTGAAAGCGGATTTAAAAAACCTCTTTGAAAAAGTGGGCGTTTATCAAAGCT
TTGATCTAGAAATTTTAGAAAAGTTTTTACTGGAGCGCTTTCATAATTTAATAGATTGTAACCCCTCTATTATAAGGAGC
GTGTTAAACACCAACGAGCGAGATATTGTTAAAATCATCCAGAAAGTCAAAGCCTTAAAACGCTTTTTAGACGATCCTAA
GAACGCTCAAAAAAAAGAGTTGCTTTTTAGCGCTTTCAAACGCCTAGCCAATATCAATAAAGACAGAAACCCTAACGAGT
CAAGCGAGTTTTCTATAAGTCTTTTCAAAGAATTACAAGAGCATGCCCTTTTTGAAGCGTTCAATGCGATCCAAACAAGC
GCTTTTGAGGGTTTGGATAGCAAAATAGAGGCTTATTTTGGTTTGCATGCGCCTTTAGAAGAATATTTTAAAAGCGTGCT
AGTCATGGATAAAGATATAGAAATCCAAAAAAATCGTAAAAATTTCTTGTGGGGCGTGTATCAAAGTTTCTTAGAGATTG
GGGATATTAAAGAAATTGCGATTTAA

Upstream 100 bases:

>100_bases
TTTGTATTAAGAAATAAACTAAACTGAGTGTAAAATCAAGTATAATTCTAACATGATTTAGTCTTAAAAGTGATTTTGTT
TTTTAGGGAGAATTGAGATT

Downstream 100 bases:

>100_bases
TTCTTTCAAACGCAAAGTCCGGCGTTTTTGTTTGGGGGTAGCTTTTTTAATAAGCGGTGCGGGCACGCTTGATGCGAAAA
TTTTTACCCTACAAGAGTTT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALLNKRALEAGNIEIFYTPRRLCLLIKDFPLLTQETKEEFFGPPIKIA
FNNEDKTQGLNALGLGFYQKLGLKDHQHFQTAFKNNKEVLYHAKIHEKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKS
FIRPIHNICVLFNGENFNGIEVKEYGFKTKQATKVHRQEGFDFIEVDSPKAYFEVLEKNHVILDPKKREAKILQEIKELE
TKHDIIVEIDRDLLDEVVAITEYPSALLGEFDKAFLKLPNEIITTSMKENQRYFAVFDQKSQEESPTLHNGFIVVSNAIN
KDKQKIIAGNQKVLKARLSDAVFFYENDLKKPLDNTPLESVVFVQGLGTLKDKMEREAIIAQYLTQKYAPSLNMPLEKAL
ELIGRAVKIAKADLLSEVVYEFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSAIVALSLKLDSLFS
LFSAGKIPSGSKDPFALRRLSFGLLKIVAHYGLEFDLKADLKNLFEKVGVYQSFDLEILEKFLLERFHNLIDCNPSIIRS
VLNTNERDIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKRLANINKDRNPNESSEFSISLFKELQEHALFEAFNAIQTS
AFEGLDSKIEAYFGLHAPLEEYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI

Sequences:

>Translated_701_residues
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALLNKRALEAGNIEIFYTPRRLCLLIKDFPLLTQETKEEFFGPPIKIA
FNNEDKTQGLNALGLGFYQKLGLKDHQHFQTAFKNNKEVLYHAKIHEKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKS
FIRPIHNICVLFNGENFNGIEVKEYGFKTKQATKVHRQEGFDFIEVDSPKAYFEVLEKNHVILDPKKREAKILQEIKELE
TKHDIIVEIDRDLLDEVVAITEYPSALLGEFDKAFLKLPNEIITTSMKENQRYFAVFDQKSQEESPTLHNGFIVVSNAIN
KDKQKIIAGNQKVLKARLSDAVFFYENDLKKPLDNTPLESVVFVQGLGTLKDKMEREAIIAQYLTQKYAPSLNMPLEKAL
ELIGRAVKIAKADLLSEVVYEFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSAIVALSLKLDSLFS
LFSAGKIPSGSKDPFALRRLSFGLLKIVAHYGLEFDLKADLKNLFEKVGVYQSFDLEILEKFLLERFHNLIDCNPSIIRS
VLNTNERDIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKRLANINKDRNPNESSEFSISLFKELQEHALFEAFNAIQTS
AFEGLDSKIEAYFGLHAPLEEYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI
>Mature_701_residues
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALLNKRALEAGNIEIFYTPRRLCLLIKDFPLLTQETKEEFFGPPIKIA
FNNEDKTQGLNALGLGFYQKLGLKDHQHFQTAFKNNKEVLYHAKIHEKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKS
FIRPIHNICVLFNGENFNGIEVKEYGFKTKQATKVHRQEGFDFIEVDSPKAYFEVLEKNHVILDPKKREAKILQEIKELE
TKHDIIVEIDRDLLDEVVAITEYPSALLGEFDKAFLKLPNEIITTSMKENQRYFAVFDQKSQEESPTLHNGFIVVSNAIN
KDKQKIIAGNQKVLKARLSDAVFFYENDLKKPLDNTPLESVVFVQGLGTLKDKMEREAIIAQYLTQKYAPSLNMPLEKAL
ELIGRAVKIAKADLLSEVVYEFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSAIVALSLKLDSLFS
LFSAGKIPSGSKDPFALRRLSFGLLKIVAHYGLEFDLKADLKNLFEKVGVYQSFDLEILEKFLLERFHNLIDCNPSIIRS
VLNTNERDIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKRLANINKDRNPNESSEFSISLFKELQEHALFEAFNAIQTS
AFEGLDSKIEAYFGLHAPLEEYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=717, Percent_Identity=30.8228730822873, Blast_Score=292, Evalue=4e-80,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80366; Mature: 80366

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALLNKRALEAGNIEIFYTPRRLCLLIKD
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHC
FPLLTQETKEEFFGPPIKIAFNNEDKTQGLNALGLGFYQKLGLKDHQHFQTAFKNNKEVL
CCHHHHHHHHHHCCCCEEEEECCCCHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
YHAKIHEKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKSFIRPIHNICVLFNGENFNGI
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCC
EVKEYGFKTKQATKVHRQEGFDFIEVDSPKAYFEVLEKNHVILDPKKREAKILQEIKELE
CHHHCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHH
TKHDIIVEIDRDLLDEVVAITEYPSALLGEFDKAFLKLPNEIITTSMKENQRYFAVFDQK
CCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCC
SQEESPTLHNGFIVVSNAINKDKQKIIAGNQKVLKARLSDAVFFYENDLKKPLDNTPLES
CCCCCCCCCCCEEEEECCCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCCCCCCHHH
VVFVQGLGTLKDKMEREAIIAQYLTQKYAPSLNMPLEKALELIGRAVKIAKADLLSEVVY
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSAIVALSLKLDSLFS
HHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LFSAGKIPSGSKDPFALRRLSFGLLKIVAHYGLEFDLKADLKNLFEKVGVYQSFDLEILE
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCHHHHH
KFLLERFHNLIDCNPSIIRSVLNTNERDIVKIIQKVKALKRFLDDPKNAQKKELLFSAFK
HHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RLANINKDRNPNESSEFSISLFKELQEHALFEAFNAIQTSAFEGLDSKIEAYFGLHAPLE
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHH
EYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALLNKRALEAGNIEIFYTPRRLCLLIKD
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHC
FPLLTQETKEEFFGPPIKIAFNNEDKTQGLNALGLGFYQKLGLKDHQHFQTAFKNNKEVL
CCHHHHHHHHHHCCCCEEEEECCCCHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
YHAKIHEKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKSFIRPIHNICVLFNGENFNGI
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCC
EVKEYGFKTKQATKVHRQEGFDFIEVDSPKAYFEVLEKNHVILDPKKREAKILQEIKELE
CHHHCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHH
TKHDIIVEIDRDLLDEVVAITEYPSALLGEFDKAFLKLPNEIITTSMKENQRYFAVFDQK
CCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCC
SQEESPTLHNGFIVVSNAINKDKQKIIAGNQKVLKARLSDAVFFYENDLKKPLDNTPLES
CCCCCCCCCCCEEEEECCCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCCCCCCHHH
VVFVQGLGTLKDKMEREAIIAQYLTQKYAPSLNMPLEKALELIGRAVKIAKADLLSEVVY
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSAIVALSLKLDSLFS
HHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LFSAGKIPSGSKDPFALRRLSFGLLKIVAHYGLEFDLKADLKNLFEKVGVYQSFDLEILE
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCHHHHH
KFLLERFHNLIDCNPSIIRSVLNTNERDIVKIIQKVKALKRFLDDPKNAQKKELLFSAFK
HHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RLANINKDRNPNESSEFSISLFKELQEHALFEAFNAIQTSAFEGLDSKIEAYFGLHAPLE
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHH
EYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA