BacMap

Definition	Burkholderia cenocepacia AU 1054 chromosome 1, complete sequence.
Accession	NC_008060
Length	3,294,563

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The map label for this gene is benD [H]

Identifier: 107022855

GI number: 107022855

Start: 1433479

End: 1434255

Strand: Reverse

Name: benD [H]

Synonym: Bcen_1303

Alternate gene names: 107022855

Gene position: 1434255-1433479 (Counterclockwise)

Preceding gene: 107022856

Following gene: 107022854

Centisome position: 43.53

GC content: 69.5

Gene sequence:

>777_bases
ATGACCATGCAACGATTCTCCGGCAAGGTCGTCGTCGTCACTGGCGCGGCGCAGGGCATCGGCCGCGGTGTCGCGCTGCG
CGCGGCGGCCGAGGGCGGCAAGGTGCTGTTCGTCGATCGCGCGGATTTCGTCGCCGACGTGGCGGCCGAGGCGACCGGTG
GCGAGACGGCAGGCTTCGTCGCCGATCTCGAAACCTATGAGGGCGCGCACGCGTCGATCGCGTATGCAGTGCAACGGTTC
GGCGGCATCGACATCCTGATCAACGGCGTCGGCGGCGCGATTCGCATGCGGCCGTTCGCCGAGTTCGAACCGGCGCAGAT
CGACGCGGAAATCCGCCGCTCGCTGATGCCGACGCTCTATGCCTGTCACGCGACGCTGCCGCACCTGATCGCGCGCGGCG
GCGGCACGATCGTCAACATCTCGTCGAACGCGACGCGCGGCATTCGCCGCGTGCCGTATTCGGCGGCGAAGGGCGGCGTG
AACGCGCTGACGTCGGCGCTCGCGATGGAATATGCGGAACACAACATCCGCGTCGTCGCCACCGCGCCGGGCGGCACCAG
CGCGCCGCCGCGCCGCGTGCCGCGCAATGCGGCCGGCGACACCGAGCAGGAACAGGCGTGGATGGGCGAGGCCGTGCGGC
AGGTGACGGAATCGACGTACTTCAAGCGCTACGGCAGCCTCGACGAGCAGATCGCGCCGATCCTGTTTCTCGCGTCGGAC
GAGGCGAGCTACATCACCGGTACGGTGCTGCCGGTCGCGGGCGGCGATACGGGTTGA

Upstream 100 bases:

>100_bases
CGATGGTCGATGCGGTGCGCCAGTATTTCGACGACGCAGGCGTGAAGCCTAACAGCTTCCACTACGAGAAGTTCACTCCC
AACGTCACGGCAAAGGCGGC

Downstream 100 bases:

>100_bases
AGGGCGTTGACGGTGCACGCGCCGCAGCCGCGTGCACGACGACCCTCACATAACCGGCCGGCGCGGCAAGCGCGGCCACG
GAGACATTCATGAGCAAACG

Product: 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase

Products: NA

Alternate protein names: 2-hydro-1,2-dihydroxybenzoate dehydrogenase; DHB dehydrogenase; Cis-1,2-dihydroxy-3,4-cyclohexadiene-1-carboxylate dehydrogenase; Cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTMQRFSGKVVVVTGAAQGIGRGVALRAAAEGGKVLFVDRADFVADVAAEATGGETAGFVADLETYEGAHASIAYAVQRF
GGIDILINGVGGAIRMRPFAEFEPAQIDAEIRRSLMPTLYACHATLPHLIARGGGTIVNISSNATRGIRRVPYSAAKGGV
NALTSALAMEYAEHNIRVVATAPGGTSAPPRRVPRNAAGDTEQEQAWMGEAVRQVTESTYFKRYGSLDEQIAPILFLASD
EASYITGTVLPVAGGDTG

Sequences:

>Translated_258_residues
MTMQRFSGKVVVVTGAAQGIGRGVALRAAAEGGKVLFVDRADFVADVAAEATGGETAGFVADLETYEGAHASIAYAVQRF
GGIDILINGVGGAIRMRPFAEFEPAQIDAEIRRSLMPTLYACHATLPHLIARGGGTIVNISSNATRGIRRVPYSAAKGGV
NALTSALAMEYAEHNIRVVATAPGGTSAPPRRVPRNAAGDTEQEQAWMGEAVRQVTESTYFKRYGSLDEQIAPILFLASD
EASYITGTVLPVAGGDTG
>Mature_257_residues
TMQRFSGKVVVVTGAAQGIGRGVALRAAAEGGKVLFVDRADFVADVAAEATGGETAGFVADLETYEGAHASIAYAVQRFG
GIDILINGVGGAIRMRPFAEFEPAQIDAEIRRSLMPTLYACHATLPHLIARGGGTIVNISSNATRGIRRVPYSAAKGGVN
ALTSALAMEYAEHNIRVVATAPGGTSAPPRRVPRNAAGDTEQEQAWMGEAVRQVTESTYFKRYGSLDEQIAPILFLASDE
ASYITGTVLPVAGGDTG

Specific function: Degradation of 2-hydro-1,2-dihydroxy benzoate (DHB) to catechol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=262, Percent_Identity=32.0610687022901, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI7705925, Length=260, Percent_Identity=29.6153846153846, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI40254992, Length=253, Percent_Identity=28.0632411067194, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI66933014, Length=256, Percent_Identity=29.6875, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI126723750, Length=260, Percent_Identity=28.4615384615385, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=26.6409266409266, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI15277342, Length=266, Percent_Identity=27.0676691729323, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI40254986, Length=181, Percent_Identity=27.0718232044199, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4504505, Length=196, Percent_Identity=31.1224489795918, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI10190704, Length=267, Percent_Identity=31.0861423220974, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI5031737, Length=260, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1789208, Length=261, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=31.0344827586207, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790717, Length=255, Percent_Identity=29.0196078431373, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787905, Length=256, Percent_Identity=26.953125, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082160, Length=264, Percent_Identity=28.4090909090909, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787335, Length=252, Percent_Identity=28.1746031746032, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI2367175, Length=265, Percent_Identity=27.1698113207547, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788459, Length=256, Percent_Identity=28.125, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789057, Length=263, Percent_Identity=28.8973384030418, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=27.6923076923077, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=263, Percent_Identity=29.277566539924, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=276, Percent_Identity=31.5217391304348, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71994600, Length=255, Percent_Identity=31.3725490196078, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=255, Percent_Identity=27.0588235294118, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17544670, Length=266, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=257, Percent_Identity=29.9610894941634, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17559104, Length=195, Percent_Identity=32.3076923076923, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=254, Percent_Identity=25.5905511811024, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17538480, Length=192, Percent_Identity=31.25, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=197, Percent_Identity=30.4568527918782, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17562904, Length=196, Percent_Identity=31.1224489795918, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=202, Percent_Identity=31.6831683168317, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=275, Percent_Identity=27.6363636363636, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=200, Percent_Identity=31.5, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=195, Percent_Identity=28.7179487179487, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=271, Percent_Identity=27.6752767527675, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=270, Percent_Identity=26.2962962962963, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=193, Percent_Identity=30.0518134715026, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=279, Percent_Identity=29.0322580645161, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=258, Percent_Identity=27.1317829457364, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=192, Percent_Identity=29.6875, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=206, Percent_Identity=29.126213592233, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=274, Percent_Identity=29.5620437956204, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562990, Length=270, Percent_Identity=27.037037037037, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI72000259, Length=264, Percent_Identity=26.8939393939394, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=265, Percent_Identity=27.1698113207547, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324126, Length=262, Percent_Identity=28.6259541984733, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357041, Length=266, Percent_Identity=31.9548872180451, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI23397609, Length=262, Percent_Identity=31.6793893129771, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24644339, Length=264, Percent_Identity=30.6818181818182, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28571526, Length=265, Percent_Identity=30.188679245283, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24639444, Length=261, Percent_Identity=27.9693486590038, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24644337, Length=262, Percent_Identity=30.9160305343511, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24643142, Length=252, Percent_Identity=25.7936507936508, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI18859837, Length=200, Percent_Identity=31.5, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21357673, Length=193, Percent_Identity=25.9067357512953, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.25 [H]

Molecular weight: Translated: 26943; Mature: 26812

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMQRFSGKVVVVTGAAQGIGRGVALRAAAEGGKVLFVDRADFVADVAAEATGGETAGFV
CCCCHHCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEECHHHHHHHHHHCCCCCCCCCE
ADLETYEGAHASIAYAVQRFGGIDILINGVGGAIRMRPFAEFEPAQIDAEIRRSLMPTLY
EEHHHHCCCHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCEECHHHHHHHHHHHH
ACHATLPHLIARGGGTIVNISSNATRGIRRVPYSAAKGGVNALTSALAMEYAEHNIRVVA
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCHHHCCCHHHHHHHHHHHHHHCCEEEEE
TAPGGTSAPPRRVPRNAAGDTEQEQAWMGEAVRQVTESTYFKRYGSLDEQIAPILFLASD
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
EASYITGTVLPVAGGDTG
CCCEEEEEEEEECCCCCC
>Mature Secondary Structure 
TMQRFSGKVVVVTGAAQGIGRGVALRAAAEGGKVLFVDRADFVADVAAEATGGETAGFV
CCCHHCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEECHHHHHHHHHHCCCCCCCCCE
ADLETYEGAHASIAYAVQRFGGIDILINGVGGAIRMRPFAEFEPAQIDAEIRRSLMPTLY
EEHHHHCCCHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCEECHHHHHHHHHHHH
ACHATLPHLIARGGGTIVNISSNATRGIRRVPYSAAKGGVNALTSALAMEYAEHNIRVVA
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCHHHCCCHHHHHHHHHHHHHHCCEEEEE
TAPGGTSAPPRRVPRNAAGDTEQEQAWMGEAVRQVTESTYFKRYGSLDEQIAPILFLASD
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
EASYITGTVLPVAGGDTG
CCCEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1740120 [H]