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The map label for this gene is dusB [H]

Identifier: 107021769

GI number: 107021769

Start: 222294

End: 223361

Strand: Reverse

Name: dusB [H]

Synonym: Bcen_0209

Alternate gene names: 107021769

Gene position: 223361-222294 (Counterclockwise)

Preceding gene: 107021770

Following gene: 107021768

Centisome position: 6.78

GC content: 66.39

Gene sequence:

>1068_bases
ATGCCCGTTATCGGCCCTCACGTATTGCGTAACAACCTGTTCGTCGCCCCGATGGCCGGGGTGACGGATCGTCCGTTCCG
CCAGCTGTGCAAGCGGCTGGGGGCCGGTTACGCCGTGTCCGAGATGGTCGCGTCCAACGCGCAGCTGTGGAAGAGCGCGA
AGACGATGCGGCGCGCGAACCACGAAGGCGAAGTCGAGCCGATCGCGGTGCAGATCGCCGGCGCCGATCCGGCGATGATG
GCCGAAGCGGCCCGCTACAACGTCGACAACGGCGCGCAGATCATCGACATCAACATGGGCTGCCCGGCGAAGAAGGTCTG
CAACGTCGCGGCCGGCTCCGCGCTGCTGCAGAACGAGCCGCTCGTGCAGCGGATCGTCGAGGCGGTCGTCGCGGCGGTCG
GCACGGGGCCCGATGCGGTGCCCGTCACGCTGAAGATCCGCACCGGCTGGGACCGCGAGCACAAGAACGCGATCACGGTC
GCGCGCCTGGCCGAAGCGGCCGGCATCTCGATGCTCACCGTGCATGGCCGCACACGCGCCGACCTGTACCGCGGCGACGC
CGAGTACGACACCATCGCGGCCGTGAAGGCGGCCGTGCGGATTCCGGTGGTCGCGAACGGCGACATCACGTCGCCCGCCA
AGGCGAAGGCCGTGCTCGATGCAACCGGCGCCGACGCGCTGATGATCGGTCGTGCCGCGCAAGGCCGGCCGTGGTTGTTC
CGCGAAATCGATCATTTCCTGCAAACCGGCGAGCTGCTGCCCCCGCCGCGGATCGACGAGATCCAGCACGTGATGAACGA
ACACCTGGAAGACCACTACGCTTTCTATGGCGAATTCACCGGTGTGCGTACTGCGCGCAAGCACATCGGCTGGTACACTC
GCGGCCTTTCCGGTGCCAACGGGTTCCGGCACCGAATGAACACGCTCGATTCCACCCGCGAGCAGCTCGCCGCCGTCAAT
GCTTTCTTCGAGGCGCAAAAGGCGCTCTCGGACCACCTCGTCTACGTCGATGACGAAGCGGAGAACAGCCAGGGCGAGTC
GGACGACCATAACCAGTTAGCAGCATGA

Upstream 100 bases:

>100_bases
GCAAACCGGGTCAAATCGGCGGGGCGTGGGAATACGGTAAAATGCCCGTTTTCCGTCTTCCCTTCCCCTGCCCGCGCTTG
TCGTGGGCGGCGCCATTGCG

Downstream 100 bases:

>100_bases
GCAAGCACAACATCGAACAATGTGTCCGCGAGAGCCTGGACGTGTATTTCCGGGATCTAGACGGCTCCAATCCGCACGAC
GTCTATGAAATGGTGATGTC

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MPVIGPHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSAKTMRRANHEGEVEPIAVQIAGADPAMM
AEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITV
ARLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPAKAKAVLDATGADALMIGRAAQGRPWLF
REIDHFLQTGELLPPPRIDEIQHVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLDSTREQLAAVN
AFFEAQKALSDHLVYVDDEAENSQGESDDHNQLAA

Sequences:

>Translated_355_residues
MPVIGPHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSAKTMRRANHEGEVEPIAVQIAGADPAMM
AEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITV
ARLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPAKAKAVLDATGADALMIGRAAQGRPWLF
REIDHFLQTGELLPPPRIDEIQHVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLDSTREQLAAVN
AFFEAQKALSDHLVYVDDEAENSQGESDDHNQLAA
>Mature_354_residues
PVIGPHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSAKTMRRANHEGEVEPIAVQIAGADPAMMA
EAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITVA
RLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPAKAKAVLDATGADALMIGRAAQGRPWLFR
EIDHFLQTGELLPPPRIDEIQHVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLDSTREQLAAVNA
FFEAQKALSDHLVYVDDEAENSQGESDDHNQLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=284, Percent_Identity=30.6338028169014, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI239788462, Length=288, Percent_Identity=30.9027777777778, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=33.7719298245614, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=33.0472103004292, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8923374, Length=253, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=57.9439252336449, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=31.5352697095436, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693211, Length=243, Percent_Identity=27.5720164609054, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=286, Percent_Identity=27.972027972028, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=33.7899543378995, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=234, Percent_Identity=31.6239316239316, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17510279, Length=254, Percent_Identity=29.1338582677165, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323433, Length=270, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=261, Percent_Identity=28.735632183908, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=231, Percent_Identity=27.7056277056277, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=292, Percent_Identity=30.8219178082192, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24580595, Length=271, Percent_Identity=30.9963099630996, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19920448, Length=271, Percent_Identity=30.9963099630996, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=29.5652173913043, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45549423, Length=255, Percent_Identity=28.6274509803922, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38488; Mature: 38357

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVIGPHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSAKTMRRAN
CCCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
HEGEVEPIAVQIAGADPAMMAEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCH
LVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITVARLAEAAGISMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHCCHHHHHHHHHHCCCEEEEECCCCHH
DLYRGDAEYDTIAAVKAAVRIPVVANGDITSPAKAKAVLDATGADALMIGRAAQGRPWLF
HHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHEEECCCCCEEEECCCCCCCCHHH
REIDHFLQTGELLPPPRIDEIQHVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFRHRMNTLDSTREQLAAVNAFFEAQKALSDHLVYVDDEAENSQGESDDHNQLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVIGPHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSAKTMRRAN
CCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
HEGEVEPIAVQIAGADPAMMAEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCH
LVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITVARLAEAAGISMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHCCHHHHHHHHHHCCCEEEEECCCCHH
DLYRGDAEYDTIAAVKAAVRIPVVANGDITSPAKAKAVLDATGADALMIGRAAQGRPWLF
HHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHEEECCCCCEEEECCCCCCCCHHH
REIDHFLQTGELLPPPRIDEIQHVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFRHRMNTLDSTREQLAAVNAFFEAQKALSDHLVYVDDEAENSQGESDDHNQLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]