| Definition | Burkholderia cenocepacia AU 1054 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008060 |
| Length | 3,294,563 |
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The map label for this gene is purH [H]
Identifier: 107021768
GI number: 107021768
Start: 220454
End: 222019
Strand: Reverse
Name: purH [H]
Synonym: Bcen_0208
Alternate gene names: 107021768
Gene position: 222019-220454 (Counterclockwise)
Preceding gene: 107021769
Following gene: 107021767
Centisome position: 6.74
GC content: 66.73
Gene sequence:
>1566_bases ATGATCAAGCAAGCGCTCATTTCCGTTTCCGACAAGACCGGCATCGTCGACTTCGCGAAGTCGCTGTCCGACCTCGGCGT CAAGCTGCTGTCGACGGGCGGCACCGCGAAACTCCTCGCCGACGCCGGCCTGCCCGTGACCGAAGTGGCCGATTACACGG GCTTCCCGGAAATGCTCGATGGGCGCGTGAAGACGCTCCACCCGAAGGTGCACGGCGGCATCCTCGCCCGCCGCGACCTG CCCGAGCACATGCAGGCGCTCGAACAGCACGGCATCCCGACGATCGACCTGCTCGTCGTGAACCTGTATCCGTTCGTCGC GACGATCGCGAAGGACGACTGCACGCTCGCCGATGCGATCGAGAACATCGACATCGGCGGCCCGACGATGCTGCGCTCGG CCGCGAAGAACCACCGCGACGTGACGGTCGTCGTCGATCCGGCCGACTACGCGGTCGTGCTCGACGAAATGAAGGCGAAC GGCAACACGGTCGGCCACCCGACCAACTTCCGCCTCGCGACGAAGGTGTTCGCGCACACCGCGCAATACGACGGCGCGAT CACGAACTACCTGACGAGCCTGACCGACGAGCTGAAGCACGCATCGCGCAGCACGTACCCGGCCACGCTGAACCTGGCGT TCGACAAGGTGCAGGACCTGCGCTACGGCGAGAACCCGCACCAGAGCGCGGCGTTCTACCGCGACCTCGCGACGCCGGCC GGCGCGCTGGCGAACTACCGCCAGCTGCAGGGCAAGGAACTGTCGTACAACAACATCGCGGATTCGGACGCGGCGTGGGA ATGCGTGAAGACGTTCGACGCGCCGGCCTGCGTGATCATCAAGCATGCGAACCCGTGTGGTGTCGCGGTGGGCAACGACT CGGCCGACGCATACGCGAAGGCGTTCCAGACCGATCCGACCTCGGCATTCGGCGGCATCATCGCGTTCAACCGCGAAGTC GACGAAGCGGCCGCGCAGGCGGTGGCGAAGCAGTTCGTCGAAGTGCTGATCGCCCCGTCGTTCTCCGACGCCGCGAAGCA GGTGTTCGCCGCGAAGCAGAACGTGCGCCTGCTCGAGATTGCACTGGGTGACGGCCACAACGCATTCGACCTGAAGCGCG TCGGCGGCGGCCTGCTCGTGCAATCGCTCGATTCGCGCAACGTGCAGCCGAGCGAACTGCGCGTCGTCACGAAGCGCCAG CCGACCGCGAAGGAAATGGACGACCTGCTGTTCGCGTGGCGCGTCGCGAAATACGTGAAGTCGAACGCGATCGTGTTCTG CGGCAACGGCATGACGCTCGGCGTCGGCGCCGGCCAGATGAGCCGCGTCGATTCCGCGCGCATCGCGAGCATCAAGGCAC AGAACGCGGGCCTGACGCTGGCCGGCTCGGCCGTCGCGTCGGATGCGTTCTTCCCGTTCCGCGACGGCCTCGACGTCGTC GTGGCGGCCGGCGCGACCTGCGTGATCCAGCCGGGCGGCTCGATGCGCGACGACGAAGTGATCGCGGCGGCCGACGAGCA CGGTATCGCGATGGTCCTGACGGGCGTGCGCCACTTCCGTCACTGA
Upstream 100 bases:
>100_bases TCGGCATCAATCGCAATACGCTGCGCAAGAAGCTGCAGCAGCACGGCCTGCTGTAGGCGGCCGTCCCGTCCCCGTTTCCC TGGCTATTGGTGGTTCCATC
Downstream 100 bases:
>100_bases TCGCCGCATGCGGCCCGCGCGGCCGCTGCACCGGAACCCGGCGGCATCATCCCCGCCGGGTTTTTTATTGCGCGGCGCCT TCGCGCGCGAACGGGCGACC
Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
Products: NA
Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]
Number of amino acids: Translated: 521; Mature: 521
Protein sequence:
>521_residues MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL PEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN GNTVGHPTNFRLATKVFAHTAQYDGAITNYLTSLTDELKHASRSTYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGDGHNAFDLKRVGGGLLVQSLDSRNVQPSELRVVTKRQ PTAKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV VAAGATCVIQPGGSMRDDEVIAAADEHGIAMVLTGVRHFRH
Sequences:
>Translated_521_residues MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL PEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN GNTVGHPTNFRLATKVFAHTAQYDGAITNYLTSLTDELKHASRSTYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGDGHNAFDLKRVGGGLLVQSLDSRNVQPSELRVVTKRQ PTAKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV VAAGATCVIQPGGSMRDDEVIAAADEHGIAMVLTGVRHFRH >Mature_521_residues MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL PEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN GNTVGHPTNFRLATKVFAHTAQYDGAITNYLTSLTDELKHASRSTYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGDGHNAFDLKRVGGGLLVQSLDSRNVQPSELRVVTKRQ PTAKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV VAAGATCVIQPGGSMRDDEVIAAADEHGIAMVLTGVRHFRH
Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]
COG id: COG0138
COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the purH family [H]
Homologues:
Organism=Homo sapiens, GI20127454, Length=478, Percent_Identity=37.4476987447699, Blast_Score=270, Evalue=3e-72, Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=62.0952380952381, Blast_Score=656, Evalue=0.0, Organism=Caenorhabditis elegans, GI71985564, Length=612, Percent_Identity=32.843137254902, Blast_Score=288, Evalue=6e-78, Organism=Caenorhabditis elegans, GI71985574, Length=318, Percent_Identity=25.7861635220126, Blast_Score=86, Evalue=5e-17, Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=33.0769230769231, Blast_Score=73, Evalue=3e-13, Organism=Saccharomyces cerevisiae, GI6323056, Length=488, Percent_Identity=37.0901639344262, Blast_Score=269, Evalue=7e-73, Organism=Saccharomyces cerevisiae, GI6323768, Length=480, Percent_Identity=36.6666666666667, Blast_Score=268, Evalue=1e-72, Organism=Drosophila melanogaster, GI24649832, Length=485, Percent_Identity=38.3505154639175, Blast_Score=273, Evalue=2e-73,
Paralogues:
None
Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002695 - InterPro: IPR013982 - InterPro: IPR016193 - InterPro: IPR011607 [H]
Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]
EC number: =2.1.2.3; =3.5.4.10 [H]
Molecular weight: Translated: 55522; Mature: 55522
Theoretical pI: Translated: 6.15; Mature: 6.15
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLD CCCHHHEECCCCCCHHHHHHHHHHCCCEEECCCCCEEEHCCCCCCHHHHHHCCCCHHHHC GRVKTLHPKVHGGILARRDLPEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAI CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHH ENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKANGNTVGHPTNFRLATKVFAHT HHCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHH AQYDGAITNYLTSLTDELKHASRSTYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA HHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCCCH GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAK HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCHHHHHH AFQTDPTSAFGGIIAFNREVDEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEI HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEE ALGDGHNAFDLKRVGGGLLVQSLDSRNVQPSELRVVTKRQPTAKEMDDLLFAWRVAKYVK EECCCCCCEEHHHCCCCCEEEECCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH SNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV CCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCEEE VAAGATCVIQPGGSMRDDEVIAAADEHGIAMVLTGVRHFRH EECCCEEEECCCCCCCCCCEEEECCCCCEEHEEHHHHHHCC >Mature Secondary Structure MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLD CCCHHHEECCCCCCHHHHHHHHHHCCCEEECCCCCEEEHCCCCCCHHHHHHCCCCHHHHC GRVKTLHPKVHGGILARRDLPEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAI CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHH ENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKANGNTVGHPTNFRLATKVFAHT HHCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHH AQYDGAITNYLTSLTDELKHASRSTYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA HHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCCCH GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAK HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCHHHHHH AFQTDPTSAFGGIIAFNREVDEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEI HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEE ALGDGHNAFDLKRVGGGLLVQSLDSRNVQPSELRVVTKRQPTAKEMDDLLFAWRVAKYVK EECCCCCCEEHHHCCCCCEEEECCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH SNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV CCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCEEE VAAGATCVIQPGGSMRDDEVIAAADEHGIAMVLTGVRHFRH EECCCEEEECCCCCCCCCCEEEECCCCCEEHEEHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA