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The map label for this gene is oprM [H]

Identifier: 107021582

GI number: 107021582

Start: 20030

End: 21601

Strand: Reverse

Name: oprM [H]

Synonym: Bcen_0020

Alternate gene names: 107021582

Gene position: 21601-20030 (Counterclockwise)

Preceding gene: 107021583

Following gene: 107021573

Centisome position: 0.66

GC content: 72.01

Gene sequence:

>1572_bases
ATGCCGTACGCCTCCCTCCCGCGCGCCGTTCGCCCGCTGAGCGCCGCCGTCGCCATTGCGACGGCCGTGCTGCTCGCCGG
CTGCGCCGTCGGGCCCGACTATCACCGCCCCGACACGTCGATCCCCGCCGCGTTCAAGGAAGCGCCGGCCGGCTGGAAAG
TCGCGCAACCCGCCGATCGCACCGACCGCGGCGCGTGGTGGACGGTCTACAACGATCCGCAGCTCGATGCGCTGATCGGC
AGGCTCAACGCGTCGAACCAGACCATCGCGCAATCGGCGGCCGCCTACCGGCAGGCGCGCGCGCTCGTCACCGAGGCGCG
CGCCGCGTATTTCCCGACCGTCGGGCTGACCGCATCGGGCTCGCGCGCACGGACGCCGCGCACGTCGCTGTCGTCGGGTT
CGTCGTCGAATTTCGGCAGCGGGTCGACCGGATCGATCAACAACAGCTACAGCGTCGGCCTCGACGCCAGTTGGGAACCC
GACCTGTGGGGCAAGGTGAGCCGCACCGTCAGCGCGCAGCGCGCCGGCGAAGCGGCCGCCGCGGCCGACCTCGCGAACGC
GCGGCTGTCGCAGCAGGCGCTGCTCGCGCAGACCTATTTCCAGTTGCGTACGTCCGATGCGCTGCAAAAGCTGCTCGACG
ACACCGTGAAGTCGTATGAACGCTCGCTGCAGCTCACGCAGAACCAGTACGCGCAGGGCGTCGCCGCGCGCGCCGACGTG
ATCCAGGCGCAAACGCAGCTGCAGAGCGCGCAGGCCGCGCAGATCGACAACGGCGTCGCCCGCGCGCAGTACGAGCATGC
GATCGCGACGCTGATCGGCGAGCCCGCGTCGACCTTCTCGCTGCCGCCGAATCCGCTCGCGGCCGAACCGCCGGTCACGC
CGGTCGACGTGCCGTCCGCGATCCTCGAGCGCCGGCCCGACATCGCGGCGGCCGAGCGCCGCGCGGCGGCCGCGAACGAG
CAGATCGGCGTCGCGATCTCGGCGTTTTTCCCGACGCTCACGTTGTCGGCCACCGGCGGCTCCCAGAGCTCGGTGTGGTC
GCAACTGTTCACGCTGCCGGCGCGCTTCTGGACGGTCGGCCCGCAGCTCGCGGCCACGCTGTTCGACGCAGGGCTGCGCG
CCGCGCAGACCGACGCCGCGCGCGCGACCTACGACCAGGACGTCGCCGCCTATCGCCTCGCGGTGCTCACCGCGTTCCAG
GACGTCGAGGACAACCTCGCGTCGCAGCGCATTCTCGCGCAGGAAATCGACGTGCAGCGGCAGGCCGTCGACAGCGCCGA
GCACGCGCTCGCGATCGTCACGAACCAGTACAAGGCCGGCACGGTCGCGTACCTGAACGTGCTGACCGCGCAGACCACCG
CGTTCACCGCGCAGCAAAAGCTCGCGACGATCGCCGGGCAGCGGATGGTGTCGTCGGTCGGGCTCGTGAAGGCGCTCGGC
GGCGGCTGGAACGTATCGGAGATGGCGCGCGAGAACGGCGACATGGCGGCGCCGGCGCCCTTGCCGGCGTCCGGTACGGC
CGCGCCCGCGGCCACCGCACCGTCGGCCGCCGCGCCGCTCGCGCAGAAGTAG

Upstream 100 bases:

>100_bases
CAGCTTTCCCGCCGTCAGTTACCATTTGTCCGCCAGCGGCATGACGACCGCATCGCGCGGCGCCGCTCGCCGGCCGTCAC
GCCCAGACAGGAAAACCACC

Downstream 100 bases:

>100_bases
AAAGGGAAGCGGGCGACGCGTCCAATCAGAATCAGCGCTGCGCGTCGCCGAAGATCAGCGACACCTGGTTGTTGCCGATC
GGGTGGCCGAGCAGGTTCAG

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MPYASLPRAVRPLSAAVAIATAVLLAGCAVGPDYHRPDTSIPAAFKEAPAGWKVAQPADRTDRGAWWTVYNDPQLDALIG
RLNASNQTIAQSAAAYRQARALVTEARAAYFPTVGLTASGSRARTPRTSLSSGSSSNFGSGSTGSINNSYSVGLDASWEP
DLWGKVSRTVSAQRAGEAAAAADLANARLSQQALLAQTYFQLRTSDALQKLLDDTVKSYERSLQLTQNQYAQGVAARADV
IQAQTQLQSAQAAQIDNGVARAQYEHAIATLIGEPASTFSLPPNPLAAEPPVTPVDVPSAILERRPDIAAAERRAAAANE
QIGVAISAFFPTLTLSATGGSQSSVWSQLFTLPARFWTVGPQLAATLFDAGLRAAQTDAARATYDQDVAAYRLAVLTAFQ
DVEDNLASQRILAQEIDVQRQAVDSAEHALAIVTNQYKAGTVAYLNVLTAQTTAFTAQQKLATIAGQRMVSSVGLVKALG
GGWNVSEMARENGDMAAPAPLPASGTAAPAATAPSAAAPLAQK

Sequences:

>Translated_523_residues
MPYASLPRAVRPLSAAVAIATAVLLAGCAVGPDYHRPDTSIPAAFKEAPAGWKVAQPADRTDRGAWWTVYNDPQLDALIG
RLNASNQTIAQSAAAYRQARALVTEARAAYFPTVGLTASGSRARTPRTSLSSGSSSNFGSGSTGSINNSYSVGLDASWEP
DLWGKVSRTVSAQRAGEAAAAADLANARLSQQALLAQTYFQLRTSDALQKLLDDTVKSYERSLQLTQNQYAQGVAARADV
IQAQTQLQSAQAAQIDNGVARAQYEHAIATLIGEPASTFSLPPNPLAAEPPVTPVDVPSAILERRPDIAAAERRAAAANE
QIGVAISAFFPTLTLSATGGSQSSVWSQLFTLPARFWTVGPQLAATLFDAGLRAAQTDAARATYDQDVAAYRLAVLTAFQ
DVEDNLASQRILAQEIDVQRQAVDSAEHALAIVTNQYKAGTVAYLNVLTAQTTAFTAQQKLATIAGQRMVSSVGLVKALG
GGWNVSEMARENGDMAAPAPLPASGTAAPAATAPSAAAPLAQK
>Mature_522_residues
PYASLPRAVRPLSAAVAIATAVLLAGCAVGPDYHRPDTSIPAAFKEAPAGWKVAQPADRTDRGAWWTVYNDPQLDALIGR
LNASNQTIAQSAAAYRQARALVTEARAAYFPTVGLTASGSRARTPRTSLSSGSSSNFGSGSTGSINNSYSVGLDASWEPD
LWGKVSRTVSAQRAGEAAAAADLANARLSQQALLAQTYFQLRTSDALQKLLDDTVKSYERSLQLTQNQYAQGVAARADVI
QAQTQLQSAQAAQIDNGVARAQYEHAIATLIGEPASTFSLPPNPLAAEPPVTPVDVPSAILERRPDIAAAERRAAAANEQ
IGVAISAFFPTLTLSATGGSQSSVWSQLFTLPARFWTVGPQLAATLFDAGLRAAQTDAARATYDQDVAAYRLAVLTAFQD
VEDNLASQRILAQEIDVQRQAVDSAEHALAIVTNQYKAGTVAYLNVLTAQTTAFTAQQKLATIAGQRMVSSVGLVKALGG
GWNVSEMARENGDMAAPAPLPASGTAAPAATAPSAAAPLAQK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=481, Percent_Identity=29.1060291060291, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI2367348, Length=489, Percent_Identity=25.7668711656442, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54631; Mature: 54500

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYASLPRAVRPLSAAVAIATAVLLAGCAVGPDYHRPDTSIPAAFKEAPAGWKVAQPADR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
TDRGAWWTVYNDPQLDALIGRLNASNQTIAQSAAAYRQARALVTEARAAYFPTVGLTASG
CCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
SRARTPRTSLSSGSSSNFGSGSTGSINNSYSVGLDASWEPDLWGKVSRTVSAQRAGEAAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCHHHH
AADLANARLSQQALLAQTYFQLRTSDALQKLLDDTVKSYERSLQLTQNQYAQGVAARADV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQAQTQLQSAQAAQIDNGVARAQYEHAIATLIGEPASTFSLPPNPLAAEPPVTPVDVPSA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ILERRPDIAAAERRAAAANEQIGVAISAFFPTLTLSATGGSQSSVWSQLFTLPARFWTVG
HHHCCCCHHHHHHHHHHCCCCCCEEHEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
PQLAATLFDAGLRAAQTDAARATYDQDVAAYRLAVLTAFQDVEDNLASQRILAQEIDVQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVDSAEHALAIVTNQYKAGTVAYLNVLTAQTTAFTAQQKLATIAGQRMVSSVGLVKALG
HHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGWNVSEMARENGDMAAPAPLPASGTAAPAATAPSAAAPLAQK
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PYASLPRAVRPLSAAVAIATAVLLAGCAVGPDYHRPDTSIPAAFKEAPAGWKVAQPADR
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
TDRGAWWTVYNDPQLDALIGRLNASNQTIAQSAAAYRQARALVTEARAAYFPTVGLTASG
CCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
SRARTPRTSLSSGSSSNFGSGSTGSINNSYSVGLDASWEPDLWGKVSRTVSAQRAGEAAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCHHHH
AADLANARLSQQALLAQTYFQLRTSDALQKLLDDTVKSYERSLQLTQNQYAQGVAARADV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQAQTQLQSAQAAQIDNGVARAQYEHAIATLIGEPASTFSLPPNPLAAEPPVTPVDVPSA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ILERRPDIAAAERRAAAANEQIGVAISAFFPTLTLSATGGSQSSVWSQLFTLPARFWTVG
HHHCCCCHHHHHHHHHHCCCCCCEEHEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
PQLAATLFDAGLRAAQTDAARATYDQDVAAYRLAVLTAFQDVEDNLASQRILAQEIDVQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVDSAEHALAIVTNQYKAGTVAYLNVLTAQTTAFTAQQKLATIAGQRMVSSVGLVKALG
HHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGWNVSEMARENGDMAAPAPLPASGTAAPAATAPSAAAPLAQK
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]