Click here to switch to the map view.

The map label for this gene is pbpE [H]

Identifier: 103488473

GI number: 103488473

Start: 3138741

End: 3140180

Strand: Direct

Name: pbpE [H]

Synonym: Sala_2996

Alternate gene names: 103488473

Gene position: 3138741-3140180 (Clockwise)

Preceding gene: 103488472

Following gene: 103488480

Centisome position: 93.83

GC content: 68.82

Gene sequence:

>1440_bases
ATGTCCAAACGATCGACCACGACAGGGCTGGCTATCCTCGCGCTATGCGCCGCGGCGCCGCCCGCAAGCGCCGAAACCGG
CGCGGAGGCCGCGGCGATCGCGCAGCGACTTCTCGACACGCTGCGCGAAACCAATGGCGTGCCCGGCATGGCTGCCGCGA
TATGGCAGGACGGCCGCATCGTCTGGGATGGTGCCAGCGGACAGCGCGATCTTGCCAGCGGCGCCCCGGTCACGCGCGAC
ACCCTGTTCCGGCTGGCGAGCGTCTCGAAGGTCTTCACCGTCGCTGCGGTCGCGCAGCTCGCCGAACAAGGCCGGATCGA
CCTCGACGCGCCGGTGCAGCGTCAGTTGCCGTGGCTCGGTGCGAACTGGCCCGCGATCACCCCGCGCCAGCTCGCCGCGC
ATGTCTCCGGCCTGCCGCATTACCAGCCGGTCGATGCAAACCGTGGATCGCACCACTATGCCACCGGGCGCGATGCCGTT
GCCATCTTCGCCCGGCGCGACCTGCTCAGCCATCCGGGAACCGCCTACAGCTATTCCTCGTGGGGCTACACGCTGCTCGG
CGCGATGGTCGAAGAAGCGGCGGGGCGCTCCTTCCCCGACTATGTGGCGGCCGAAGTCGCGCCGGGGCTGGCAATCCGTG
CCGACGCGACCAACAGCGACGATCCCCATGCCTCGCGCGCCTATGCCTTCGATCGGGGCCGCGTCGTCGAAGCGCCGCGT
CACGATTATAGCTACACATGGGGCGGCGGCGGGCTCGGCGGCACGGCGAGCGCGGTCGCCGCTTTCGGCGGCCGAATGAT
CGAGGGCCGCGTCGTGCGCCCCGAAAGTTTCGACGCGATGCTGGTGCCGGTGCGGCTCGCCAACGGCAATGCTGCCGGAG
AGGATGGCTACGCCGTCGGTTTCGGATGGCGCATCGGTGTCGATTCGGACGATGCCCCTTTTGTCCATCATAATGGCGTC
ACGCTCGGTGCGCGCAGCACGATCGGGCTGTGGCGCGGCGAACGCACCGCGGTCAGCCTGCTTTCCAACGCCGCGTGGAT
ATCGCGCATGGAACCCACCGCGCAGATGATCGCCGCCCCCTTCCGCCCGGTGCCAACCGGGCTTGTGGATGCCGGCTGCC
CGCTTGAAGCGCGGCGCTACAGCGGCACGTTGGGAGACACGGTGGTCGAAGGCGTCGTTCGCTTCAGGGTCGAACAGGGG
CTGTGCATCGGGACCGTCGATCAGCATCCGGCCATGCGCGCCTATTTCGGTTCCGCGATGCAGCGCACCGACGCAGCGCT
GACGATCATCGGGTTCGATCCAAAGGGGCACCTCGCGCGCGGAGGACTCGTCCACCCCTTCGGCATCGCCGATCTCCGCG
CCCGAACCGACGGCAGTTTCGTCGCCGATGTCACTGCGGGGCGGCGGCTGGTGCTGCGCTTCGATCAGGCGGGTAGATAG

Upstream 100 bases:

>100_bases
ATAGGACCGCGCACCATCACTTCGCGACAGGATTGCGACATATCGGCAAATCGCTGCGACATCGCCGACCGATATCTCCC
CGCGACAAGGGAGGGACCGG

Downstream 100 bases:

>100_bases
CGGCGTGTGACCAACTCCCGCTCGAGCGCCGCCGCATCAACGAAACGGTGGCCCGCGATCCCCGCCGTTTCGGCGCCTGC
GACATTTGCCGCGACATCGT

Product: beta-lactamase

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSKRSTTTGLAILALCAAAPPASAETGAEAAAIAQRLLDTLRETNGVPGMAAAIWQDGRIVWDGASGQRDLASGAPVTRD
TLFRLASVSKVFTVAAVAQLAEQGRIDLDAPVQRQLPWLGANWPAITPRQLAAHVSGLPHYQPVDANRGSHHYATGRDAV
AIFARRDLLSHPGTAYSYSSWGYTLLGAMVEEAAGRSFPDYVAAEVAPGLAIRADATNSDDPHASRAYAFDRGRVVEAPR
HDYSYTWGGGGLGGTASAVAAFGGRMIEGRVVRPESFDAMLVPVRLANGNAAGEDGYAVGFGWRIGVDSDDAPFVHHNGV
TLGARSTIGLWRGERTAVSLLSNAAWISRMEPTAQMIAAPFRPVPTGLVDAGCPLEARRYSGTLGDTVVEGVVRFRVEQG
LCIGTVDQHPAMRAYFGSAMQRTDAALTIIGFDPKGHLARGGLVHPFGIADLRARTDGSFVADVTAGRRLVLRFDQAGR

Sequences:

>Translated_479_residues
MSKRSTTTGLAILALCAAAPPASAETGAEAAAIAQRLLDTLRETNGVPGMAAAIWQDGRIVWDGASGQRDLASGAPVTRD
TLFRLASVSKVFTVAAVAQLAEQGRIDLDAPVQRQLPWLGANWPAITPRQLAAHVSGLPHYQPVDANRGSHHYATGRDAV
AIFARRDLLSHPGTAYSYSSWGYTLLGAMVEEAAGRSFPDYVAAEVAPGLAIRADATNSDDPHASRAYAFDRGRVVEAPR
HDYSYTWGGGGLGGTASAVAAFGGRMIEGRVVRPESFDAMLVPVRLANGNAAGEDGYAVGFGWRIGVDSDDAPFVHHNGV
TLGARSTIGLWRGERTAVSLLSNAAWISRMEPTAQMIAAPFRPVPTGLVDAGCPLEARRYSGTLGDTVVEGVVRFRVEQG
LCIGTVDQHPAMRAYFGSAMQRTDAALTIIGFDPKGHLARGGLVHPFGIADLRARTDGSFVADVTAGRRLVLRFDQAGR
>Mature_478_residues
SKRSTTTGLAILALCAAAPPASAETGAEAAAIAQRLLDTLRETNGVPGMAAAIWQDGRIVWDGASGQRDLASGAPVTRDT
LFRLASVSKVFTVAAVAQLAEQGRIDLDAPVQRQLPWLGANWPAITPRQLAAHVSGLPHYQPVDANRGSHHYATGRDAVA
IFARRDLLSHPGTAYSYSSWGYTLLGAMVEEAAGRSFPDYVAAEVAPGLAIRADATNSDDPHASRAYAFDRGRVVEAPRH
DYSYTWGGGGLGGTASAVAAFGGRMIEGRVVRPESFDAMLVPVRLANGNAAGEDGYAVGFGWRIGVDSDDAPFVHHNGVT
LGARSTIGLWRGERTAVSLLSNAAWISRMEPTAQMIAAPFRPVPTGLVDAGCPLEARRYSGTLGDTVVEGVVRFRVEQGL
CIGTVDQHPAMRAYFGSAMQRTDAALTIIGFDPKGHLARGGLVHPFGIADLRARTDGSFVADVTAGRRLVLRFDQAGR

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI26051231, Length=129, Percent_Identity=34.8837209302326, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI26051233, Length=129, Percent_Identity=34.8837209302326, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1790593, Length=224, Percent_Identity=30.3571428571429, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115533026, Length=349, Percent_Identity=26.0744985673352, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 50608; Mature: 50476

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRSTTTGLAILALCAAAPPASAETGAEAAAIAQRLLDTLRETNGVPGMAAAIWQDGRI
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCEE
VWDGASGQRDLASGAPVTRDTLFRLASVSKVFTVAAVAQLAEQGRIDLDAPVQRQLPWLG
EEECCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
ANWPAITPRQLAAHVSGLPHYQPVDANRGSHHYATGRDAVAIFARRDLLSHPGTAYSYSS
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCEEECC
WGYTLLGAMVEEAAGRSFPDYVAAEVAPGLAIRADATNSDDPHASRAYAFDRGRVVEAPR
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCEEECCC
HDYSYTWGGGGLGGTASAVAAFGGRMIEGRVVRPESFDAMLVPVRLANGNAAGEDGYAVG
CCCCEECCCCCCCHHHHHHHHHCCEEEECEEECCCCCCEEEEEEEEECCCCCCCCCEEEE
FGWRIGVDSDDAPFVHHNGVTLGARSTIGLWRGERTAVSLLSNAAWISRMEPTAQMIAAP
EEEEECCCCCCCCEEEECCEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHCC
FRPVPTGLVDAGCPLEARRYSGTLGDTVVEGVVRFRVEQGLCIGTVDQHPAMRAYFGSAM
CCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
QRTDAALTIIGFDPKGHLARGGLVHPFGIADLRARTDGSFVADVTAGRRLVLRFDQAGR
HHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHEECCCCCEEEEECCCCEEEEEECCCCC
>Mature Secondary Structure 
SKRSTTTGLAILALCAAAPPASAETGAEAAAIAQRLLDTLRETNGVPGMAAAIWQDGRI
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCEE
VWDGASGQRDLASGAPVTRDTLFRLASVSKVFTVAAVAQLAEQGRIDLDAPVQRQLPWLG
EEECCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
ANWPAITPRQLAAHVSGLPHYQPVDANRGSHHYATGRDAVAIFARRDLLSHPGTAYSYSS
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCEEECC
WGYTLLGAMVEEAAGRSFPDYVAAEVAPGLAIRADATNSDDPHASRAYAFDRGRVVEAPR
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCEEECCC
HDYSYTWGGGGLGGTASAVAAFGGRMIEGRVVRPESFDAMLVPVRLANGNAAGEDGYAVG
CCCCEECCCCCCCHHHHHHHHHCCEEEECEEECCCCCCEEEEEEEEECCCCCCCCCEEEE
FGWRIGVDSDDAPFVHHNGVTLGARSTIGLWRGERTAVSLLSNAAWISRMEPTAQMIAAP
EEEEECCCCCCCCEEEECCEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHCC
FRPVPTGLVDAGCPLEARRYSGTLGDTVVEGVVRFRVEQGLCIGTVDQHPAMRAYFGSAM
CCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
QRTDAALTIIGFDPKGHLARGGLVHPFGIADLRARTDGSFVADVTAGRRLVLRFDQAGR
HHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHEECCCCCEEEEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]