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The map label for this gene is thiC

Identifier: 103487851

GI number: 103487851

Start: 2492752

End: 2494644

Strand: Direct

Name: thiC

Synonym: Sala_2370

Alternate gene names: 103487851

Gene position: 2492752-2494644 (Clockwise)

Preceding gene: 103487847

Following gene: 103487852

Centisome position: 74.52

GC content: 66.09

Gene sequence:

>1893_bases
ATGGCCGACATCGACAGCCGCCTCGACACGACGCAAGCCACCCCCATCGGCGTCACCACCGGGCCGATCCGCGGCAGCCG
CAAGATCCACGTCGCCAGCCCCACCGGCAGCGGCATCCGCGTCGCGATGCGCGAGATCCTGCTCGAACCCTCATCGGGCG
AACCGCCGGTGCGCGTCTACGACACCAGCGGGCCTTATACGGACCCCGATGCGACGATCGACATCGCCCAGGGCCTCCCC
GAACTGCGGGCAAGCTGGATCCGCGCCCGCGGCGACGTCGAAGAGGTGGCCCAGCGCGAGGTTCGGCCGGAGGACAACGG
CCAGCTCGGCCCCGACCGTTCGGGCGGCGTCCCCGCCTTCCCCAATGTCCGCAAGAAAGTGCTGCGCGCCAAGCCCGGCG
CGAACGTCAGCCAGATGCACTATGCCCGCCGCGGCATCATCACGCCCGAGATGGAATATGTCGCGACGCGCGAGAATCTC
GGCCGCGAACGGCTCGCCGAATATGTCCGCGACGGGCAGGACTGGGGCGCCAGCATCCCCGACTATGTCACCCCCGAATT
CGTCCGCGACGAGGTCGCGCGCGGCCGCGCGATCATCCCCAGCAACATCAACCACCCCGAAAGCGAGCCGATGGCGATCG
GCCGCAACTTCCTCGTCAAGATCAACGCCAATATCGGCAACAGCGCGGTCGCCAGCGACGTCGCGGCCGAGGTCGACAAG
ATGGTCTGGTCGATCCGCTGGGGCGCCGACACCGTCATGGACCTGTCGACCGGGCGCAACATCCACGACACGCGCGAATG
GATCCTCCGCAACTCGCCCGTCCCGATCGGCACCGTCCCCATCTATCAGGCGCTCGAAAAGGTCGGCGGCATCGCCGAGG
AACTGACGTGGGAAATCTTCCGCGACACGCTGATCGAACAGGCCGAACAGGGCGTCGACTATTTCACCATCCATGCGGGT
GTCCGTCTGCCTTACGTGCCCCTCGCCGCGAAGCGCGTCACCGGCATCGTCAGCCGCGGCGGCAGCATCATGGCGAAATG
GTGCCTCGCGCATCACAGGGAAAGCTTCCTCTACGACCATTTCGACGAGATTACCGAGATCATGAAGGCCTATGACATCG
CCTACAGCCTCGGCGACGGCCTGCGCCCCGGCAGCATCGCCGACGCGAATGACGAGGCGCAGTTTGCCGAGCTCTACACG
CTGGGCGAGCTTACCAGGCGCGCGTGGGCACAGGATGTGCAGGTGATGATCGAGGGACCGGGGCATGTTCCCATGCACAA
GATCAAGGAGAATATGGACAAGCAGCTCGAGGCCTGCGGCGAGGCGCCCTTCTACACCCTGGGGCCGCTCACCACCGACA
TCGCGCCCGGCTACGACCATATCACCAGCGGCATCGGCGCCGCGATGATCGGCTGGTACGGCACCGCGATGCTTTGCTAC
GTCACGCCCAAGGAGCATCTGGGCCTGCCCGACCGCGACGATGTGAAGGTCGGCGTCGTCACCTACAAGCTCGCCGCCCA
CGCCGCCGACCTCGCCAAGGGCCACCCCGCCGCGCAGGTCCGCGACGATGCGCTGTCAAAGGCGCGCTTCGAGTTCCGCT
GGCGCGACCAGTTCAACCTGTCGCTCGACCCCGACACCGCCGAGCAATATCACGACCAGACCCTCCCCGCCGAGGGCGCC
AAGACCGCGCATTTCTGCAGCATGTGCGGGCCGAAGTTCTGCTCGATGAAGATCAGCCAGGAGGTGCGCGAGTTCGCGAA
GCTGCAAAATCAGGACAGCGCCGGTTTCATCGCGGCAGAAGAGGCCGAAAAGGGCATGGCGGAAATGAGTCAGGTCTATG
AGGACACGGGGCGCGAGCTGTATATGGGCGCTGGTGGGCGCGAGCATGATTGA

Upstream 100 bases:

>100_bases
CTGATCCGGGTAATACCGGCGGAGGGAGGGTTGGCTTTCACCCCAAAGCGAAACAGGGCGTGAAATCCATTCTCGCTCCG
AACCTGTGGAGCGAACAGAC

Downstream 100 bases:

>100_bases
CGCCCATCGGGCGGAGAAAGGATGACAGCCCATGCCAACCCTGCTCCGCATCCTCACCCTCGCCGCATCGCTCGCGTTCG
CCCCCGCCGCCGCCAGCGCC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MADIDSRLDTTQATPIGVTTGPIRGSRKIHVASPTGSGIRVAMREILLEPSSGEPPVRVYDTSGPYTDPDATIDIAQGLP
ELRASWIRARGDVEEVAQREVRPEDNGQLGPDRSGGVPAFPNVRKKVLRAKPGANVSQMHYARRGIITPEMEYVATRENL
GRERLAEYVRDGQDWGASIPDYVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVAAEVDK
MVWSIRWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGIAEELTWEIFRDTLIEQAEQGVDYFTIHAG
VRLPYVPLAAKRVTGIVSRGGSIMAKWCLAHHRESFLYDHFDEITEIMKAYDIAYSLGDGLRPGSIADANDEAQFAELYT
LGELTRRAWAQDVQVMIEGPGHVPMHKIKENMDKQLEACGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGTAMLCY
VTPKEHLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAQVRDDALSKARFEFRWRDQFNLSLDPDTAEQYHDQTLPAEGA
KTAHFCSMCGPKFCSMKISQEVREFAKLQNQDSAGFIAAEEAEKGMAEMSQVYEDTGRELYMGAGGREHD

Sequences:

>Translated_630_residues
MADIDSRLDTTQATPIGVTTGPIRGSRKIHVASPTGSGIRVAMREILLEPSSGEPPVRVYDTSGPYTDPDATIDIAQGLP
ELRASWIRARGDVEEVAQREVRPEDNGQLGPDRSGGVPAFPNVRKKVLRAKPGANVSQMHYARRGIITPEMEYVATRENL
GRERLAEYVRDGQDWGASIPDYVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVAAEVDK
MVWSIRWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGIAEELTWEIFRDTLIEQAEQGVDYFTIHAG
VRLPYVPLAAKRVTGIVSRGGSIMAKWCLAHHRESFLYDHFDEITEIMKAYDIAYSLGDGLRPGSIADANDEAQFAELYT
LGELTRRAWAQDVQVMIEGPGHVPMHKIKENMDKQLEACGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGTAMLCY
VTPKEHLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAQVRDDALSKARFEFRWRDQFNLSLDPDTAEQYHDQTLPAEGA
KTAHFCSMCGPKFCSMKISQEVREFAKLQNQDSAGFIAAEEAEKGMAEMSQVYEDTGRELYMGAGGREHD
>Mature_629_residues
ADIDSRLDTTQATPIGVTTGPIRGSRKIHVASPTGSGIRVAMREILLEPSSGEPPVRVYDTSGPYTDPDATIDIAQGLPE
LRASWIRARGDVEEVAQREVRPEDNGQLGPDRSGGVPAFPNVRKKVLRAKPGANVSQMHYARRGIITPEMEYVATRENLG
RERLAEYVRDGQDWGASIPDYVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVAAEVDKM
VWSIRWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGIAEELTWEIFRDTLIEQAEQGVDYFTIHAGV
RLPYVPLAAKRVTGIVSRGGSIMAKWCLAHHRESFLYDHFDEITEIMKAYDIAYSLGDGLRPGSIADANDEAQFAELYTL
GELTRRAWAQDVQVMIEGPGHVPMHKIKENMDKQLEACGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGTAMLCYV
TPKEHLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAQVRDDALSKARFEFRWRDQFNLSLDPDTAEQYHDQTLPAEGAK
TAHFCSMCGPKFCSMKISQEVREFAKLQNQDSAGFIAAEEAEKGMAEMSQVYEDTGRELYMGAGGREHD

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=617, Percent_Identity=67.5850891410049, Blast_Score=837, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_SPHAL (Q1GQJ3)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617412.1
- GeneID:   4080810
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2370
- NMPDR:   fig|317655.9.peg.2292
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   SALA317655:SALA_2370-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 69437; Mature: 69306

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: BINDING 224-224 BINDING 253-253 BINDING 282-282 BINDING 318-318 BINDING 418-418 BINDING 445-445

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIDSRLDTTQATPIGVTTGPIRGSRKIHVASPTGSGIRVAMREILLEPSSGEPPVRVY
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEE
DTSGPYTDPDATIDIAQGLPELRASWIRARGDVEEVAQREVRPEDNGQLGPDRSGGVPAF
ECCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PNVRKKVLRAKPGANVSQMHYARRGIITPEMEYVATRENLGRERLAEYVRDGQDWGASIP
CHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCHHHCCCCC
DYVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVAAEVDK
CCCCHHHHHHHHHCCCEECCCCCCCCCCCCHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
MVWSIRWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGIAEELTWEIF
HHEEEECCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
RDTLIEQAEQGVDYFTIHAGVRLPYVPLAAKRVTGIVSRGGSIMAKWCLAHHRESFLYDH
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FDEITEIMKAYDIAYSLGDGLRPGSIADANDEAQFAELYTLGELTRRAWAQDVQVMIEGP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECC
GHVPMHKIKENMDKQLEACGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGTAMLCY
CCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
VTPKEHLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAQVRDDALSKARFEFRWRDQFNL
ECCHHHCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCCE
SLDPDTAEQYHDQTLPAEGAKTAHFCSMCGPKFCSMKISQEVREFAKLQNQDSAGFIAAE
EECCCHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHH
EAEKGMAEMSQVYEDTGRELYMGAGGREHD
HHHHHHHHHHHHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure 
ADIDSRLDTTQATPIGVTTGPIRGSRKIHVASPTGSGIRVAMREILLEPSSGEPPVRVY
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEE
DTSGPYTDPDATIDIAQGLPELRASWIRARGDVEEVAQREVRPEDNGQLGPDRSGGVPAF
ECCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PNVRKKVLRAKPGANVSQMHYARRGIITPEMEYVATRENLGRERLAEYVRDGQDWGASIP
CHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCHHHCCCCC
DYVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVAAEVDK
CCCCHHHHHHHHHCCCEECCCCCCCCCCCCHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
MVWSIRWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGIAEELTWEIF
HHEEEECCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
RDTLIEQAEQGVDYFTIHAGVRLPYVPLAAKRVTGIVSRGGSIMAKWCLAHHRESFLYDH
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FDEITEIMKAYDIAYSLGDGLRPGSIADANDEAQFAELYTLGELTRRAWAQDVQVMIEGP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECC
GHVPMHKIKENMDKQLEACGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGTAMLCY
CCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
VTPKEHLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAQVRDDALSKARFEFRWRDQFNL
ECCHHHCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCCE
SLDPDTAEQYHDQTLPAEGAKTAHFCSMCGPKFCSMKISQEVREFAKLQNQDSAGFIAAE
EECCCHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHH
EAEKGMAEMSQVYEDTGRELYMGAGGREHD
HHHHHHHHHHHHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA