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The map label for this gene is prpB [C]

Identifier: 103487762

GI number: 103487762

Start: 2404553

End: 2405314

Strand: Reverse

Name: prpB [C]

Synonym: Sala_2281

Alternate gene names: 103487762

Gene position: 2405314-2404553 (Counterclockwise)

Preceding gene: 103487763

Following gene: 103487755

Centisome position: 71.9

GC content: 70.73

Gene sequence:

>762_bases
ATGACCGACGACAAAATTGCCCGTTTCCGTGCGCTGCACGTCCCCGGCGACCCGCTGATTCTCGTCAACATCTGGGATGC
GGGGAGCGCGAAGGCGGTTGCCGCGGCGGGTGCGAAGGCGATCGCGACGGGCAGCTTCGGCGTCGCGGGCGCGCAGGGAC
GCGCCGACGGCGAGGATTTCCCGCTCGAAGACGTGTTCGAAAATCTGGATCGCATCCTGTCGGTCACCGACCTGCCGGTC
ACGATCGACATGGAATCGGGCTATGGCGCCGATCCGGCGGCGGTTGGCGCCTCGGTCGGCCGCGCCAGGCACGCGGGCGC
GGCGGGGATCAACATGGAGGACCGGCTGCCCGGCCGGTCCGACCTCCTGCCCGTCGCCGAAGCGGTCGCGCGCTACCGCG
CCGCCGCCGACACCGGCATTTTCGTCAACGCACGCTGCGACACGTTTCGCGGGCAGGACGTCGCGAAGGACGGCGATGCG
CTCGTCGCCGCGACGCTCGAACGCGCGCGCGCCTATGCTGATGCGGGCGCGGGGTCGCTGTTCGTGCCCTTCCTGCTCGA
CCCCAAATGCATCGGCGCGATCTGCGACGCCTCGCCGCTGCCGGTGAACATATTGCGCGGCAAGGGCGGCCCGACGCACA
GGGAACTCGCCGACCTCGGCGTCGCGCGGATCAGCCACGGACACCAGCCCTGGGCCGCCGCGATGGCGTGGCTGGGCGCG
CAGGCGGCACAGGTTCTGGGCGGCGGCGAACCCGATTATTGA

Upstream 100 bases:

>100_bases
TGCTCATCCCGTGCCACCGCGTGGTGCGCAGCGACGGCTCGCTCGGCGGCTATGCCTATGGCCTGCCGATCAAGGAAGAA
TTGCTCAAAAGGGAAGTGAA

Downstream 100 bases:

>100_bases
TCGGCGTCGGCGGGTGCGACCGGGGCGGCGGCGCCTTTTCCGGTGCCGCCACTCCTTTCGCGCATTGATTCGATCTGTGC
CAGAATCCTCTCGCCCTGTC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTDDKIARFRALHVPGDPLILVNIWDAGSAKAVAAAGAKAIATGSFGVAGAQGRADGEDFPLEDVFENLDRILSVTDLPV
TIDMESGYGADPAAVGASVGRARHAGAAGINMEDRLPGRSDLLPVAEAVARYRAAADTGIFVNARCDTFRGQDVAKDGDA
LVAATLERARAYADAGAGSLFVPFLLDPKCIGAICDASPLPVNILRGKGGPTHRELADLGVARISHGHQPWAAAMAWLGA
QAAQVLGGGEPDY

Sequences:

>Translated_253_residues
MTDDKIARFRALHVPGDPLILVNIWDAGSAKAVAAAGAKAIATGSFGVAGAQGRADGEDFPLEDVFENLDRILSVTDLPV
TIDMESGYGADPAAVGASVGRARHAGAAGINMEDRLPGRSDLLPVAEAVARYRAAADTGIFVNARCDTFRGQDVAKDGDA
LVAATLERARAYADAGAGSLFVPFLLDPKCIGAICDASPLPVNILRGKGGPTHRELADLGVARISHGHQPWAAAMAWLGA
QAAQVLGGGEPDY
>Mature_252_residues
TDDKIARFRALHVPGDPLILVNIWDAGSAKAVAAAGAKAIATGSFGVAGAQGRADGEDFPLEDVFENLDRILSVTDLPVT
IDMESGYGADPAAVGASVGRARHAGAAGINMEDRLPGRSDLLPVAEAVARYRAAADTGIFVNARCDTFRGQDVAKDGDAL
VAATLERARAYADAGAGSLFVPFLLDPKCIGAICDASPLPVNILRGKGGPTHRELADLGVARISHGHQPWAAAMAWLGAQ
AAQVLGGGEPDY

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=207, Percent_Identity=31.4009661835749, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 26037; Mature: 25905

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDKIARFRALHVPGDPLILVNIWDAGSAKAVAAAGAKAIATGSFGVAGAQGRADGEDF
CCCCCCCCEEEEECCCCCEEEEEEECCCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCC
PLEDVFENLDRILSVTDLPVTIDMESGYGADPAAVGASVGRARHAGAAGINMEDRLPGRS
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCC
DLLPVAEAVARYRAAADTGIFVNARCDTFRGQDVAKDGDALVAATLERARAYADAGAGSL
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCE
FVPFLLDPKCIGAICDASPLPVNILRGKGGPTHRELADLGVARISHGHQPWAAAMAWLGA
EEEEEECHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QAAQVLGGGEPDY
HHHHHHCCCCCCC
>Mature Secondary Structure 
TDDKIARFRALHVPGDPLILVNIWDAGSAKAVAAAGAKAIATGSFGVAGAQGRADGEDF
CCCCCCCEEEEECCCCCEEEEEEECCCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCC
PLEDVFENLDRILSVTDLPVTIDMESGYGADPAAVGASVGRARHAGAAGINMEDRLPGRS
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCC
DLLPVAEAVARYRAAADTGIFVNARCDTFRGQDVAKDGDALVAATLERARAYADAGAGSL
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCE
FVPFLLDPKCIGAICDASPLPVNILRGKGGPTHRELADLGVARISHGHQPWAAAMAWLGA
EEEEEECHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QAAQVLGGGEPDY
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]