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The map label for this gene is thcD [H]

Identifier: 103487455

GI number: 103487455

Start: 2079098

End: 2080321

Strand: Reverse

Name: thcD [H]

Synonym: Sala_1971

Alternate gene names: 103487455

Gene position: 2080321-2079098 (Counterclockwise)

Preceding gene: 103487456

Following gene: 103487453

Centisome position: 62.19

GC content: 67.97

Gene sequence:

>1224_bases
ATGCAATTCGACGTGGTGATCGTGGGCGCCGGACACGGCGGCGCGCAGGTGGCGGTGGCGCTGCGTACGCAGAAGTTCGC
CGGATCGATCGCGATCATCGGCGACGAACCCGATCTGCCATACGAACGCCCGCCCTTGTCGAAGGAATATTTTGCGGGCG
ATAAGGACTTCGAGCGCATCCTGCTGCGCCCCGCCAGATATTGGGACGAGCGCGCGGTGACGATGCTGCTCGGTCAGCGC
GTCATGGCGGTCGATCCCGCCGCGCACAGCGTCACCACCGACGACGGACAGACGGTCGGCTATGGCAAGCTCGTCTGGGC
CACCGGCGGGAGCCCGCGGATGCTGCCCATCCCCGGCGGCGACCTGCCCGGCGTGCAGGGCGTGCGCACGCGCGCTGACG
CCGATGCGATGAAGGCGGCGTCCGAAACCGCTGGCCAGATTGTCGTCATCGGCGGCGGCTATATCGGGCTGGAGGCGGCG
GCGGTGCTCCGCAAGGCGGGCAAGAAGGTCGTGCTGCTGGAGGCGCTCGACCGTGTTCTTGCGCGCGTCGCGGGCGAGGA
GCTGTCGCGCTTTTTCGAAAAGGAGCATCGCGACCATGGCGTCGACCTGCGGCTCGGCGTGTGCGTCGAGGCGATCGAGG
GCGACACGCACGCCACCGGGGTGCGGCTGGCGGTCGGTGCGGTGATCCCCGCCGACCTCGTCATCGTCGGCATCGGCATC
GTCCCCGCGGTCGAACCGCTGATCGCGGCGGGCGCCGAGGGCGGCAACGGCGTGCTCGTCGACCGTTTGTGCAGGACGAG
CCTGCCCGACATCTATGCGATCGGCGACTGCGCCGCGCACGCCAATGATTTTGCCGGAGGCGCGGTGATCCGCCTCGAAT
CGGTCCAGAATGCCAACGACCAGGCGAATGTCGTCGCCAGGGGCATCGTCGGCGACGAGGCGCCCTATCATGCGATCCCC
TGGTTCTGGTCGAACCAATATGATCTGAAGCTTCAGACCGCGGGGCTTTCGACCGGCCACGATCAGGCGGTGCTGCGCGG
CGATCCGGCGACGCGCAGCTTCTCGGTCGTCTACCTCAAGGCGGGCAGGGTGATCGCCATCGACTGCGTCAATGCGACGA
AGGATTATGTCCAGGGGCGGATGATCGTCACCGCCGGGCTTCGCGCCACGGCGGAGCAGCTTGCCGATACCGGAACCCCG
CTGAAGGCGCTGTTGCCGGCCTGA

Upstream 100 bases:

>100_bases
GCGTTCAACTTTTTCTTTCGCCGGAAGGAGGCGAGGGCGGCTTGCCCCGCCCGGCCGCGTGTGTAACGGCCGCGCTGACG
AATGGCGGGAGAGACAGGCG

Downstream 100 bases:

>100_bases
TCCTCAGGTCGGCGGCAGTGGGTCTGTGCGGGGCGGTTCGTCGCCCATCGCCTTCCTGAGCGTCGCCTTGACGTTGCGGA
GCAGGCGGCGCAGCGCGACG

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MQFDVVIVGAGHGGAQVAVALRTQKFAGSIAIIGDEPDLPYERPPLSKEYFAGDKDFERILLRPARYWDERAVTMLLGQR
VMAVDPAAHSVTTDDGQTVGYGKLVWATGGSPRMLPIPGGDLPGVQGVRTRADADAMKAASETAGQIVVIGGGYIGLEAA
AVLRKAGKKVVLLEALDRVLARVAGEELSRFFEKEHRDHGVDLRLGVCVEAIEGDTHATGVRLAVGAVIPADLVIVGIGI
VPAVEPLIAAGAEGGNGVLVDRLCRTSLPDIYAIGDCAAHANDFAGGAVIRLESVQNANDQANVVARGIVGDEAPYHAIP
WFWSNQYDLKLQTAGLSTGHDQAVLRGDPATRSFSVVYLKAGRVIAIDCVNATKDYVQGRMIVTAGLRATAEQLADTGTP
LKALLPA

Sequences:

>Translated_407_residues
MQFDVVIVGAGHGGAQVAVALRTQKFAGSIAIIGDEPDLPYERPPLSKEYFAGDKDFERILLRPARYWDERAVTMLLGQR
VMAVDPAAHSVTTDDGQTVGYGKLVWATGGSPRMLPIPGGDLPGVQGVRTRADADAMKAASETAGQIVVIGGGYIGLEAA
AVLRKAGKKVVLLEALDRVLARVAGEELSRFFEKEHRDHGVDLRLGVCVEAIEGDTHATGVRLAVGAVIPADLVIVGIGI
VPAVEPLIAAGAEGGNGVLVDRLCRTSLPDIYAIGDCAAHANDFAGGAVIRLESVQNANDQANVVARGIVGDEAPYHAIP
WFWSNQYDLKLQTAGLSTGHDQAVLRGDPATRSFSVVYLKAGRVIAIDCVNATKDYVQGRMIVTAGLRATAEQLADTGTP
LKALLPA
>Mature_407_residues
MQFDVVIVGAGHGGAQVAVALRTQKFAGSIAIIGDEPDLPYERPPLSKEYFAGDKDFERILLRPARYWDERAVTMLLGQR
VMAVDPAAHSVTTDDGQTVGYGKLVWATGGSPRMLPIPGGDLPGVQGVRTRADADAMKAASETAGQIVVIGGGYIGLEAA
AVLRKAGKKVVLLEALDRVLARVAGEELSRFFEKEHRDHGVDLRLGVCVEAIEGDTHATGVRLAVGAVIPADLVIVGIGI
VPAVEPLIAAGAEGGNGVLVDRLCRTSLPDIYAIGDCAAHANDFAGGAVIRLESVQNANDQANVVARGIVGDEAPYHAIP
WFWSNQYDLKLQTAGLSTGHDQAVLRGDPATRSFSVVYLKAGRVIAIDCVNATKDYVQGRMIVTAGLRATAEQLADTGTP
LKALLPA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=371, Percent_Identity=29.1105121293801, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI226437568, Length=371, Percent_Identity=29.1105121293801, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI21389617, Length=371, Percent_Identity=29.1105121293801, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI22202629, Length=345, Percent_Identity=25.7971014492754, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4757732, Length=345, Percent_Identity=25.7971014492754, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI195927006, Length=174, Percent_Identity=28.1609195402299, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788892, Length=401, Percent_Identity=32.4189526184539, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789065, Length=315, Percent_Identity=26.984126984127, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789765, Length=291, Percent_Identity=29.2096219931272, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=398, Percent_Identity=27.8894472361809, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24639257, Length=341, Percent_Identity=28.1524926686217, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281359715, Length=341, Percent_Identity=28.1524926686217, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281359713, Length=341, Percent_Identity=28.1524926686217, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24639250, Length=341, Percent_Identity=28.1524926686217, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI18543267, Length=341, Percent_Identity=28.1524926686217, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24639252, Length=341, Percent_Identity=28.1524926686217, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585130, Length=341, Percent_Identity=25.8064516129032, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28573993, Length=359, Percent_Identity=24.2339832869081, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24581020, Length=359, Percent_Identity=24.2339832869081, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 42780; Mature: 42780

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDVVIVGAGHGGAQVAVALRTQKFAGSIAIIGDEPDLPYERPPLSKEYFAGDKDFERI
CCEEEEEEECCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHCCCCHHHHHH
LLRPARYWDERAVTMLLGQRVMAVDPAAHSVTTDDGQTVGYGKLVWATGGSPRMLPIPGG
HHCHHHHHHHHHHHHHHCCEEEEECCCCCCEECCCCCEECCEEEEEEECCCCEEEECCCC
DLPGVQGVRTRADADAMKAASETAGQIVVIGGGYIGLEAAAVLRKAGKKVVLLEALDRVL
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHHH
ARVAGEELSRFFEKEHRDHGVDLRLGVCVEAIEGDTHATGVRLAVGAVIPADLVIVGIGI
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCEEEEECHHCCCCEEEEECCH
VPAVEPLIAAGAEGGNGVLVDRLCRTSLPDIYAIGDCAAHANDFAGGAVIRLESVQNAND
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEECCHHHCCCCCCCCEEEEEECCCCCCC
QANVVARGIVGDEAPYHAIPWFWSNQYDLKLQTAGLSTGHDQAVLRGDPATRSFSVVYLK
CHHEEEECCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEECCCCCCEEEEEEEE
AGRVIAIDCVNATKDYVQGRMIVTAGLRATAEQLADTGTPLKALLPA
CCCEEEEEECCCCHHHHCCEEEEEECCHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure
MQFDVVIVGAGHGGAQVAVALRTQKFAGSIAIIGDEPDLPYERPPLSKEYFAGDKDFERI
CCEEEEEEECCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHCCCCHHHHHH
LLRPARYWDERAVTMLLGQRVMAVDPAAHSVTTDDGQTVGYGKLVWATGGSPRMLPIPGG
HHCHHHHHHHHHHHHHHCCEEEEECCCCCCEECCCCCEECCEEEEEEECCCCEEEECCCC
DLPGVQGVRTRADADAMKAASETAGQIVVIGGGYIGLEAAAVLRKAGKKVVLLEALDRVL
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHHH
ARVAGEELSRFFEKEHRDHGVDLRLGVCVEAIEGDTHATGVRLAVGAVIPADLVIVGIGI
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCEEEEECHHCCCCEEEEECCH
VPAVEPLIAAGAEGGNGVLVDRLCRTSLPDIYAIGDCAAHANDFAGGAVIRLESVQNAND
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEECCHHHCCCCCCCCEEEEEECCCCCCC
QANVVARGIVGDEAPYHAIPWFWSNQYDLKLQTAGLSTGHDQAVLRGDPATRSFSVVYLK
CHHEEEECCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEECCCCCCEEEEEEEE
AGRVIAIDCVNATKDYVQGRMIVTAGLRATAEQLADTGTPLKALLPA
CCCEEEEEECCCCHHHHCCEEEEEECCHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]