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The map label for this gene is mraW

Identifier: 103487373

GI number: 103487373

Start: 1988771

End: 1989748

Strand: Reverse

Name: mraW

Synonym: Sala_1889

Alternate gene names: 103487373

Gene position: 1989748-1988771 (Counterclockwise)

Preceding gene: 103487374

Following gene: 103487372

Centisome position: 59.48

GC content: 70.25

Gene sequence:

>978_bases
GTGACCGACCTTCCCCGCGATCCCCGTCACGATCCGGTCCTGCTTGATGAGGTCATCGCCGCGCTCGCCATTGCCCCGGG
CGAGCGTCATGTCGATGCGACCTTCGGCGCGGGCGGCTATACGCGCGCGATGCTCGCCGCGGGCGCCGAGGTGATTGCCT
GCGACCGCGACCCCGACGCGATCGCGGGCGGCGAGGCGCTTGTTGCGGAAGCCGACGGCCGCCTGACGCTGATCCACGGC
CGTTTCGGCGAGATCGACCGGCTGCTCGCCGAGCGCGGGATCGATGCGGTCGACGGCATCACCTTCGACATCGGCGTCTC
GTCAATGCAGCTCGACCAGGGCGAGCGCGGCTTTTCGTTCCAGAAGGACGGCCCGCTCGACATGCGTATGGCGCAGGAAG
GCGAAAGCGCCGCCGACTGGCTCAACCGCGCCGACGAATATGAGATTGCCGATGTCCTCTATCATTATGGTGACGAGCGC
CAGTCGCGGCGCGTCGCGCGCGCGATCGTCGCGGCGCGCCCGCTGACGCGTACCAGCGAACTGGCGAGTGTTGTCCGCAA
GGCGCTGCGCCACCCGCCCGGCGCGCCGAAAGACCCGGCGACAAAGAGTTTTCAGGCCATCCGCATCCACATCAACCGCG
AACTCGACGAGCTGGTCGCGGGTTTGGCCGCGGCGGAGCGTGTCTTGCGTCCAGGCGGCCGGCTCGCAGTCGTCAGCTTT
CACAGCACCGAGGACCGGATCGTCAAGCACTTCCTGCGCGAACGCAGCGGCGGCGACGCCGCGGGATCGCGCCACCGGCC
CGCGCCGACCGCCGCCGCGCGCGCCGCAACCTTTGAAACCCCGGCGCGCAAGGTGCGGCCGGGCAAGGCCGAAGAGGCGC
GCAATCCGCGCGCGCGCTCGGCGACGCTGCGCAGCGCCGTGCGCACCGCGGCGCCGGCCTGGCCGGGCAATTCGATGAAG
GAGGCAATGTCATGCTGA

Upstream 100 bases:

>100_bases
AAATCTGGTGGCTGCCGACGCTCGCCGAATGCGCCGAGGCCGACCCGGTGCTCCAGCGGCTTGCCGCCTCATGGGATGCC
GCGAAAGGGAAGGGGCGAAA

Downstream 100 bases:

>100_bases
TGGCGGCCCGCAAGCTGCAGGGGATCGGTCTGGTCCTGCTGGTGCTCCTCTTTGCGATGATGCTTTATCCCGTGTCGCTC
AAGGTTGCGGCGACGCGCAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTDLPRDPRHDPVLLDEVIAALAIAPGERHVDATFGAGGYTRAMLAAGAEVIACDRDPDAIAGGEALVAEADGRLTLIHG
RFGEIDRLLAERGIDAVDGITFDIGVSSMQLDQGERGFSFQKDGPLDMRMAQEGESAADWLNRADEYEIADVLYHYGDER
QSRRVARAIVAARPLTRTSELASVVRKALRHPPGAPKDPATKSFQAIRIHINRELDELVAGLAAAERVLRPGGRLAVVSF
HSTEDRIVKHFLRERSGGDAAGSRHRPAPTAAARAATFETPARKVRPGKAEEARNPRARSATLRSAVRTAAPAWPGNSMK
EAMSC

Sequences:

>Translated_325_residues
MTDLPRDPRHDPVLLDEVIAALAIAPGERHVDATFGAGGYTRAMLAAGAEVIACDRDPDAIAGGEALVAEADGRLTLIHG
RFGEIDRLLAERGIDAVDGITFDIGVSSMQLDQGERGFSFQKDGPLDMRMAQEGESAADWLNRADEYEIADVLYHYGDER
QSRRVARAIVAARPLTRTSELASVVRKALRHPPGAPKDPATKSFQAIRIHINRELDELVAGLAAAERVLRPGGRLAVVSF
HSTEDRIVKHFLRERSGGDAAGSRHRPAPTAAARAATFETPARKVRPGKAEEARNPRARSATLRSAVRTAAPAWPGNSMK
EAMSC
>Mature_324_residues
TDLPRDPRHDPVLLDEVIAALAIAPGERHVDATFGAGGYTRAMLAAGAEVIACDRDPDAIAGGEALVAEADGRLTLIHGR
FGEIDRLLAERGIDAVDGITFDIGVSSMQLDQGERGFSFQKDGPLDMRMAQEGESAADWLNRADEYEIADVLYHYGDERQ
SRRVARAIVAARPLTRTSELASVVRKALRHPPGAPKDPATKSFQAIRIHINRELDELVAGLAAAERVLRPGGRLAVVSFH
STEDRIVKHFLRERSGGDAAGSRHRPAPTAAARAATFETPARKVRPGKAEEARNPRARSATLRSAVRTAAPAWPGNSMKE
AMSC

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=40.9495548961424, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=42.2459893048128, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI1786270, Length=317, Percent_Identity=47.0031545741325, Blast_Score=243, Evalue=1e-65,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=38.5542168674699, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=38.5542168674699, Blast_Score=194, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_SPHAL (Q1GRX1)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616934.1
- ProteinModelPortal:   Q1GRX1
- SMR:   Q1GRX1
- GeneID:   4082634
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1889
- NMPDR:   fig|317655.9.peg.1817
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   SALA317655:SALA_1889-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35045; Mature: 34914

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 82-82 BINDING 103-103 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLPRDPRHDPVLLDEVIAALAIAPGERHVDATFGAGGYTRAMLAAGAEVIACDRDPDA
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHCCCEEEEECCCCCC
IAGGEALVAEADGRLTLIHGRFGEIDRLLAERGIDAVDGITFDIGVSSMQLDQGERGFSF
CCCCCEEEEECCCCEEEEECCHHHHHHHHHHCCCCHHHCEEEECCCCCEECCCCCCCCCC
QKDGPLDMRMAQEGESAADWLNRADEYEIADVLYHYGDERQSRRVARAIVAARPLTRTSE
CCCCCCCHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHH
LASVVRKALRHPPGAPKDPATKSFQAIRIHINRELDELVAGLAAAERVLRPGGRLAVVSF
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEE
HSTEDRIVKHFLRERSGGDAAGSRHRPAPTAAARAATFETPARKVRPGKAEEARNPRARS
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHCCCCCHHHHCCCHHHH
ATLRSAVRTAAPAWPGNSMKEAMSC
HHHHHHHHHHCCCCCCCHHHHHHCC
>Mature Secondary Structure 
TDLPRDPRHDPVLLDEVIAALAIAPGERHVDATFGAGGYTRAMLAAGAEVIACDRDPDA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHCCCEEEEECCCCCC
IAGGEALVAEADGRLTLIHGRFGEIDRLLAERGIDAVDGITFDIGVSSMQLDQGERGFSF
CCCCCEEEEECCCCEEEEECCHHHHHHHHHHCCCCHHHCEEEECCCCCEECCCCCCCCCC
QKDGPLDMRMAQEGESAADWLNRADEYEIADVLYHYGDERQSRRVARAIVAARPLTRTSE
CCCCCCCHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHH
LASVVRKALRHPPGAPKDPATKSFQAIRIHINRELDELVAGLAAAERVLRPGGRLAVVSF
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEE
HSTEDRIVKHFLRERSGGDAAGSRHRPAPTAAARAATFETPARKVRPGKAEEARNPRARS
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHCCCCCHHHHCCCHHHH
ATLRSAVRTAAPAWPGNSMKEAMSC
HHHHHHHHHHCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA