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The map label for this gene is fixI [H]

Identifier: 103487155

GI number: 103487155

Start: 1757617

End: 1759563

Strand: Reverse

Name: fixI [H]

Synonym: Sala_1670

Alternate gene names: 103487155

Gene position: 1759563-1757617 (Counterclockwise)

Preceding gene: 103487156

Following gene: 103487154

Centisome position: 52.6

GC content: 70.42

Gene sequence:

>1947_bases
ATGCCCGACCTCATCGGCGCCTTTTCGACCCTGGGGTTCGAGGCGCACCCGCTGGGCAACGGTGCAGATGCAACCGACGT
CGGTGCCGAAACGAGCCGCGAGCTGCTGCGCGCGGTCGCGGTATCGGGCTTTGCGATGATGAACATCATGCTGCTGTCGG
TGTCGATCTGGTCGGGTGCGGCGGGTGCGACGCGCGACCTGTTCCACTGGCTGTCCGCCGCGATCGCGCTGCCGACGATC
ACCTATGCCGGACGCCCGTTTTTCCGCTCGGCATGGCGCGCGCTGCGGCACGGGGGCACCAATATGGACGTTCCGATCAG
CATCGGCGTCGCGCTCGTCTGTGCTATGAGCCTTTACGAGACGGCGTCGAGCGGCCCGCATGCCTATTTCGATGGCGCGG
TGATGCTGCTCTTCTTTCTGCTCACCGGCCGCTGGCTCGACAGCGTGATGCGCGACCGCGCGCGGGGCGGAGTCGCGGCG
CTGCTGCGCAACATGGGGACGGGCGCGATGGTGATCGGGGTCGACGGGGACAGCCGATGGGTCGATGCGACGGCGCTCGA
ACCGGGGATGGAGATGCTGGTGGCGGCGGGCGAACGGCTGGCCGCCGACGGGGTGGTTGTGGCGGGCGAAAGTCGGTTCG
ACCTGTCGCTACTGACCGGAGAGAGCGCGCCGGTTGCGGCGCAAGTCGATGATCGCGTCCATGCCGGCACGCTCAATCTC
GACGCGCCGATCCGCGTGCGCGTCACTGCCGCGGGAGCCGACACCGCGATCGCGGACATCGCGCGGCTTATGGGCGAGGC
GGCGCAGGGCAAGTCGCGCCATGTCCGTATCGCCGACCGTGCCGCGCGCTTCTATGCCCCCGCGGTCCACGCGCTCGCTC
TGCTCGCTTTCGCCGGGTGGATGATCGCGGGGGCGGGCTGGCATCAAAGCCTGCTGGTCGCCGCGGCGGTGCTCATCATC
ACCTGCCCCTGCGCGCTGGGCCTCGCCGTCCCGGCGGCGCAGATCGTCGCGGCGGGCGAATTGATGCGCGCAGGCGTGCT
CGTGAAGGACGGATCGGCACTTGAACGGCTGGCCGAGGTCGACCTCGCGCTCGTCGACAAGACCGGGACGCTGACGCTTG
GGCGTCCCGAAGCGATGGGGCTCGACCGGCTGGACGCGGAGGTGCGCTCGCTGACCCTTGCGCTTGCGCGGGCGAGCCGC
CACCCGCTGAGCGCGGCGTTGACGCGCGAGCTTCAGGCAGGCGGTGTCCGGGCCGCTCGGGTGGAGGCGATCCGCGAAAC
GCCGGGCGTCGGGATCGAGGCGCGCTGGCGCGGCCGGGTCGTGACGCTGGGGCGACCGCAGGGCAGCGATCTTGGCAACG
CGCTTGCCACCGAACTGGCCGTCGACGGTCGCCCGGTCGCGACGATCACCTTTGCCGACCCTTTGCGCCCTGACGCCGCC
GAGGCGGTCGATGCCCTGGAGCGGGACGGCGTCGCAGCGATGATATTGTCGGGCGACCGCGCCGAAGCCGTCGCGCCGGT
TGCCCGCGACCTGCGCCTGACGGCGCAGACGGGGATGAGCCCGCAGGACAAGCTGGCCGCGATCGCGCGGCAGGCGGCAC
GGGGGCGCAAGGTGCTGATGATCGGCGACGGGCTCAACGACGGTCCGGCGCTCGCGGCGGGCCATGCTTCGATGGCGCCG
GGGTCGGCGAGCGATGTCGGCAAGAATGCCGCCGATTGCATCTTCCTCGGCGACAGGATGATGCCCGTGGTGCAGGCGCT
CCGTATGGCGCGGCGGACGCAGGCGGTGGTGCGGCAGAACTTTCTGCTCGCGATCGGCTATAATGTCGTCGCGGTGCCGC
TCGCCTTCATGGGGCTGGTCACGCCGCTCGTCGCGGCGATCGCCATGTCGGGATCGTCGCTGATCGTCGTCGGCAATGCG
CTGCGGCTGAAAAGGGCAATCCGGTGA

Upstream 100 bases:

>100_bases
CAAGCTGGAGCAGGGGCTGGTGCGCGACAGGCGCATCGCGACGGCACGCGTCAATTTCACCGAAAAGCGCGTGCATCTGA
CGCATACGCCCGACGCCCGG

Downstream 100 bases:

>100_bases
ACGGGCTGGCCTTGCTCATCCCGATCGCGCTGGGGCTGGGCCTGCTTGGGCTCGCGGCCTTTTTCTGGTCGCTCGGCAAG
GGACAGTTCGACGATCTTGA

Product: heavy metal translocating P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MPDLIGAFSTLGFEAHPLGNGADATDVGAETSRELLRAVAVSGFAMMNIMLLSVSIWSGAAGATRDLFHWLSAAIALPTI
TYAGRPFFRSAWRALRHGGTNMDVPISIGVALVCAMSLYETASSGPHAYFDGAVMLLFFLLTGRWLDSVMRDRARGGVAA
LLRNMGTGAMVIGVDGDSRWVDATALEPGMEMLVAAGERLAADGVVVAGESRFDLSLLTGESAPVAAQVDDRVHAGTLNL
DAPIRVRVTAAGADTAIADIARLMGEAAQGKSRHVRIADRAARFYAPAVHALALLAFAGWMIAGAGWHQSLLVAAAVLII
TCPCALGLAVPAAQIVAAGELMRAGVLVKDGSALERLAEVDLALVDKTGTLTLGRPEAMGLDRLDAEVRSLTLALARASR
HPLSAALTRELQAGGVRAARVEAIRETPGVGIEARWRGRVVTLGRPQGSDLGNALATELAVDGRPVATITFADPLRPDAA
EAVDALERDGVAAMILSGDRAEAVAPVARDLRLTAQTGMSPQDKLAAIARQAARGRKVLMIGDGLNDGPALAAGHASMAP
GSASDVGKNAADCIFLGDRMMPVVQALRMARRTQAVVRQNFLLAIGYNVVAVPLAFMGLVTPLVAAIAMSGSSLIVVGNA
LRLKRAIR

Sequences:

>Translated_648_residues
MPDLIGAFSTLGFEAHPLGNGADATDVGAETSRELLRAVAVSGFAMMNIMLLSVSIWSGAAGATRDLFHWLSAAIALPTI
TYAGRPFFRSAWRALRHGGTNMDVPISIGVALVCAMSLYETASSGPHAYFDGAVMLLFFLLTGRWLDSVMRDRARGGVAA
LLRNMGTGAMVIGVDGDSRWVDATALEPGMEMLVAAGERLAADGVVVAGESRFDLSLLTGESAPVAAQVDDRVHAGTLNL
DAPIRVRVTAAGADTAIADIARLMGEAAQGKSRHVRIADRAARFYAPAVHALALLAFAGWMIAGAGWHQSLLVAAAVLII
TCPCALGLAVPAAQIVAAGELMRAGVLVKDGSALERLAEVDLALVDKTGTLTLGRPEAMGLDRLDAEVRSLTLALARASR
HPLSAALTRELQAGGVRAARVEAIRETPGVGIEARWRGRVVTLGRPQGSDLGNALATELAVDGRPVATITFADPLRPDAA
EAVDALERDGVAAMILSGDRAEAVAPVARDLRLTAQTGMSPQDKLAAIARQAARGRKVLMIGDGLNDGPALAAGHASMAP
GSASDVGKNAADCIFLGDRMMPVVQALRMARRTQAVVRQNFLLAIGYNVVAVPLAFMGLVTPLVAAIAMSGSSLIVVGNA
LRLKRAIR
>Mature_647_residues
PDLIGAFSTLGFEAHPLGNGADATDVGAETSRELLRAVAVSGFAMMNIMLLSVSIWSGAAGATRDLFHWLSAAIALPTIT
YAGRPFFRSAWRALRHGGTNMDVPISIGVALVCAMSLYETASSGPHAYFDGAVMLLFFLLTGRWLDSVMRDRARGGVAAL
LRNMGTGAMVIGVDGDSRWVDATALEPGMEMLVAAGERLAADGVVVAGESRFDLSLLTGESAPVAAQVDDRVHAGTLNLD
APIRVRVTAAGADTAIADIARLMGEAAQGKSRHVRIADRAARFYAPAVHALALLAFAGWMIAGAGWHQSLLVAAAVLIIT
CPCALGLAVPAAQIVAAGELMRAGVLVKDGSALERLAEVDLALVDKTGTLTLGRPEAMGLDRLDAEVRSLTLALARASRH
PLSAALTRELQAGGVRAARVEAIRETPGVGIEARWRGRVVTLGRPQGSDLGNALATELAVDGRPVATITFADPLRPDAAE
AVDALERDGVAAMILSGDRAEAVAPVARDLRLTAQTGMSPQDKLAAIARQAARGRKVLMIGDGLNDGPALAAGHASMAPG
SASDVGKNAADCIFLGDRMMPVVQALRMARRTQAVVRQNFLLAIGYNVVAVPLAFMGLVTPLVAAIAMSGSSLIVVGNAL
RLKRAIR

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=637, Percent_Identity=27.7864992150706, Blast_Score=226, Evalue=5e-59,
Organism=Homo sapiens, GI115529486, Length=384, Percent_Identity=29.9479166666667, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI55743073, Length=556, Percent_Identity=26.7985611510791, Blast_Score=168, Evalue=2e-41,
Organism=Escherichia coli, GI1786691, Length=608, Percent_Identity=33.2236842105263, Blast_Score=266, Evalue=4e-72,
Organism=Escherichia coli, GI1789879, Length=579, Percent_Identity=32.9879101899827, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI1786914, Length=471, Percent_Identity=26.5392781316348, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556548, Length=659, Percent_Identity=25.7966616084977, Blast_Score=195, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6319772, Length=662, Percent_Identity=26.1329305135952, Blast_Score=200, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6320475, Length=609, Percent_Identity=26.1083743842365, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6325221, Length=517, Percent_Identity=22.8239845261122, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321430, Length=517, Percent_Identity=22.8239845261122, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221329854, Length=428, Percent_Identity=31.5420560747664, Blast_Score=169, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 67340; Mature: 67209

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00154 ATPASE_E1_E2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLIGAFSTLGFEAHPLGNGADATDVGAETSRELLRAVAVSGFAMMNIMLLSVSIWSGA
CCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGATRDLFHWLSAAIALPTITYAGRPFFRSAWRALRHGGTNMDVPISIGVALVCAMSLYE
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TASSGPHAYFDGAVMLLFFLLTGRWLDSVMRDRARGGVAALLRNMGTGAMVIGVDGDSRW
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCE
VDATALEPGMEMLVAAGERLAADGVVVAGESRFDLSLLTGESAPVAAQVDDRVHAGTLNL
EEHHHCCCHHHHHHHCCCHHCCCCEEEECCCCCEEEEEECCCCCEEEECCCCEECCEECC
DAPIRVRVTAAGADTAIADIARLMGEAAQGKSRHVRIADRAARFYAPAVHALALLAFAGW
CCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
MIAGAGWHQSLLVAAAVLIITCPCALGLAVPAAQIVAAGELMRAGVLVKDGSALERLAEV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHC
DLALVDKTGTLTLGRPEAMGLDRLDAEVRSLTLALARASRHPLSAALTRELQAGGVRAAR
CEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHH
VEAIRETPGVGIEARWRGRVVTLGRPQGSDLGNALATELAVDGRPVATITFADPLRPDAA
HHHHHHCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHEECCCCEEEEEECCCCCCCHH
EAVDALERDGVAAMILSGDRAEAVAPVARDLRLTAQTGMSPQDKLAAIARQAARGRKVLM
HHHHHHHHCCEEEEEECCCCHHHHHHHHHHHEEHHHCCCCHHHHHHHHHHHHHCCCEEEE
IGDGLNDGPALAAGHASMAPGSASDVGKNAADCIFLGDRMMPVVQALRMARRTQAVVRQN
EECCCCCCCCEECCCCCCCCCCCHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHC
FLLAIGYNVVAVPLAFMGLVTPLVAAIAMSGSSLIVVGNALRLKRAIR
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCC
>Mature Secondary Structure 
PDLIGAFSTLGFEAHPLGNGADATDVGAETSRELLRAVAVSGFAMMNIMLLSVSIWSGA
CCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGATRDLFHWLSAAIALPTITYAGRPFFRSAWRALRHGGTNMDVPISIGVALVCAMSLYE
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TASSGPHAYFDGAVMLLFFLLTGRWLDSVMRDRARGGVAALLRNMGTGAMVIGVDGDSRW
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCE
VDATALEPGMEMLVAAGERLAADGVVVAGESRFDLSLLTGESAPVAAQVDDRVHAGTLNL
EEHHHCCCHHHHHHHCCCHHCCCCEEEECCCCCEEEEEECCCCCEEEECCCCEECCEECC
DAPIRVRVTAAGADTAIADIARLMGEAAQGKSRHVRIADRAARFYAPAVHALALLAFAGW
CCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
MIAGAGWHQSLLVAAAVLIITCPCALGLAVPAAQIVAAGELMRAGVLVKDGSALERLAEV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHC
DLALVDKTGTLTLGRPEAMGLDRLDAEVRSLTLALARASRHPLSAALTRELQAGGVRAAR
CEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHH
VEAIRETPGVGIEARWRGRVVTLGRPQGSDLGNALATELAVDGRPVATITFADPLRPDAA
HHHHHHCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHEECCCCEEEEEECCCCCCCHH
EAVDALERDGVAAMILSGDRAEAVAPVARDLRLTAQTGMSPQDKLAAIARQAARGRKVLM
HHHHHHHHCCEEEEEECCCCHHHHHHHHHHHEEHHHCCCCHHHHHHHHHHHHHCCCEEEE
IGDGLNDGPALAAGHASMAPGSASDVGKNAADCIFLGDRMMPVVQALRMARRTQAVVRQN
EECCCCCCCCEECCCCCCCCCCCHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHC
FLLAIGYNVVAVPLAFMGLVTPLVAAIAMSGSSLIVVGNALRLKRAIR
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA