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The map label for this gene is purF [H]

Identifier: 103487026

GI number: 103487026

Start: 1607527

End: 1609035

Strand: Direct

Name: purF [H]

Synonym: Sala_1541

Alternate gene names: 103487026

Gene position: 1607527-1609035 (Clockwise)

Preceding gene: 103487020

Following gene: 103487027

Centisome position: 48.06

GC content: 64.81

Gene sequence:

>1509_bases
TTGCCCTCCCTATATGCGCGGCAGTCACTGCCCCCCTGCCGCTGGAACGCCATGCTGACGACCCATCCTTTCGACGACGA
CAAGCTCCGCGAGGAGTGCGGCGTCTTTGGCATTCACGGCGCCGACAGCGCTTCGGCGGTCGTTGCGCTGGGTCTCCACG
CGCTCCAGCACCGCGGACAGGAAGCCGCGGGCATCACCGCCTTCGATGGCAAGGAGTTTCACACCCACCGCGCGATGGGG
CATGTCGCCGGCAATTTCGATCGCGACGAGATCATCCGTCAATTGGACGGCGCTTCGGCGGTCGGTCACGTCCGCTATTC
GACCACCGGCGAAACGGCCCTGCGCAACGTCCAGCCGCTGTTCGCCGACCTGTCGACGGGCGGTTTTGCGATCGCGCACA
ACGGCAATATCTCGAACGCCGCCGCGCTCCGCAAGCTGCTCGTCCGCCGCGGTTCGATCTTTCAGTCGACCAGCGATACC
GAGGTGATCATCCACCTCGTCGCGACCTCGAGTTACCGCTCGCTGCTCGACCGTTTCATCGACGCGCTGAAACAGGTCGA
GGGCGCCTATTCGCTCATCTGCCTGACCGCCGAGGGCATGATCGGCTGCCGCGACCCGCTCGGCATCCGCCCGCTCGTGA
TCGGCAAGCTCGGCGACGCGCACATCCTCGCGTCCGAAACGGTGGCGCTCGACGTCGTCGGTGCGGAGTTCCTGCGTTCG
GTCGAACCGGGCGAGCTCATCATCATCCGCGACGGCCAGCTCACCTCGCACCGTCCCTTTGCCGAACAATCGGCGCGGCC
GTGCATTTTCGAATATGTCTATTTCTCGCGCCCCGATTCGATCGTCGACGGCACCAGCGTCTATTCGGTGCGCAAGGCGA
TCGGCGCCGAACTCGCGCGCGAAAATCCGGTCGATGCCGACCTGGTCATCCCGGTCCCCGATTCGGGCACGCCCGCCGCG
ATCGGCTATGCGCAGGAATCGGGCATCCCGTTCGAGCTTGGCATCATCCGTTCGCACTATGTCGGACGCACCTTCATCCA
GCCGGGCGACAAGGTCCGCCACCTTGGCGTCAAATTGAAGCACAACGCCAACCGCGCGCTGATCGCGGGCCAGCGCATCG
TGCTGATCGACGATTCGATCGTGCGCGGCACGACGAGCCTCAAGATCGTGCAGATGATGCGCGATGCCGGGGCGGCGGAA
GTGCATATGCGCATCGCCAGCCCGCCGACCTCGCACAGCTGCTTCTATGGCGTCGACACGCCCGAGCGCGCCAAGCTGCT
CGCGGCGCAGATGACGATCGGCCAGATGGCGAACTTCATCAATGCCGACAGCCTCGCCTTCCTGACCATCGACGGCCTCT
ATCGCGCGCTGGGCGAGGCGGATCGCAAACAGGACGCGCCGCAATATTGCGACGCCTGCTTCACCGGCGACTATCCGACC
ACGCTGACCGACTTCGACGAGCATGGGCTGGAGGATCAATTTTCGCTGCTTGCCGAACGGGTTGTCTGA

Upstream 100 bases:

>100_bases
TCATCCATGCCCGCCGCTTTAGAGCAGGATCGAAGCCGCTGTCACCCCGCGCGTGACCGCCGATGTGACAATATCGTGAT
TAGGGGGGCGACAATCGCCT

Downstream 100 bases:

>100_bases
CATAATGGTTACAAGGTCTGAAGAACTGGCGGGGCAGATCGCGCTCGTCACCGGGGCAAGCCGTGGTATCGGCGCCGCAA
TCGCCGAATCGCTGGCCGCA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MPSLYARQSLPPCRWNAMLTTHPFDDDKLREECGVFGIHGADSASAVVALGLHALQHRGQEAAGITAFDGKEFHTHRAMG
HVAGNFDRDEIIRQLDGASAVGHVRYSTTGETALRNVQPLFADLSTGGFAIAHNGNISNAAALRKLLVRRGSIFQSTSDT
EVIIHLVATSSYRSLLDRFIDALKQVEGAYSLICLTAEGMIGCRDPLGIRPLVIGKLGDAHILASETVALDVVGAEFLRS
VEPGELIIIRDGQLTSHRPFAEQSARPCIFEYVYFSRPDSIVDGTSVYSVRKAIGAELARENPVDADLVIPVPDSGTPAA
IGYAQESGIPFELGIIRSHYVGRTFIQPGDKVRHLGVKLKHNANRALIAGQRIVLIDDSIVRGTTSLKIVQMMRDAGAAE
VHMRIASPPTSHSCFYGVDTPERAKLLAAQMTIGQMANFINADSLAFLTIDGLYRALGEADRKQDAPQYCDACFTGDYPT
TLTDFDEHGLEDQFSLLAERVV

Sequences:

>Translated_502_residues
MPSLYARQSLPPCRWNAMLTTHPFDDDKLREECGVFGIHGADSASAVVALGLHALQHRGQEAAGITAFDGKEFHTHRAMG
HVAGNFDRDEIIRQLDGASAVGHVRYSTTGETALRNVQPLFADLSTGGFAIAHNGNISNAAALRKLLVRRGSIFQSTSDT
EVIIHLVATSSYRSLLDRFIDALKQVEGAYSLICLTAEGMIGCRDPLGIRPLVIGKLGDAHILASETVALDVVGAEFLRS
VEPGELIIIRDGQLTSHRPFAEQSARPCIFEYVYFSRPDSIVDGTSVYSVRKAIGAELARENPVDADLVIPVPDSGTPAA
IGYAQESGIPFELGIIRSHYVGRTFIQPGDKVRHLGVKLKHNANRALIAGQRIVLIDDSIVRGTTSLKIVQMMRDAGAAE
VHMRIASPPTSHSCFYGVDTPERAKLLAAQMTIGQMANFINADSLAFLTIDGLYRALGEADRKQDAPQYCDACFTGDYPT
TLTDFDEHGLEDQFSLLAERVV
>Mature_501_residues
PSLYARQSLPPCRWNAMLTTHPFDDDKLREECGVFGIHGADSASAVVALGLHALQHRGQEAAGITAFDGKEFHTHRAMGH
VAGNFDRDEIIRQLDGASAVGHVRYSTTGETALRNVQPLFADLSTGGFAIAHNGNISNAAALRKLLVRRGSIFQSTSDTE
VIIHLVATSSYRSLLDRFIDALKQVEGAYSLICLTAEGMIGCRDPLGIRPLVIGKLGDAHILASETVALDVVGAEFLRSV
EPGELIIIRDGQLTSHRPFAEQSARPCIFEYVYFSRPDSIVDGTSVYSVRKAIGAELARENPVDADLVIPVPDSGTPAAI
GYAQESGIPFELGIIRSHYVGRTFIQPGDKVRHLGVKLKHNANRALIAGQRIVLIDDSIVRGTTSLKIVQMMRDAGAAEV
HMRIASPPTSHSCFYGVDTPERAKLLAAQMTIGQMANFINADSLAFLTIDGLYRALGEADRKQDAPQYCDACFTGDYPTT
LTDFDEHGLEDQFSLLAERVV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=508, Percent_Identity=37.007874015748, Blast_Score=339, Evalue=4e-93,
Organism=Escherichia coli, GI1788651, Length=475, Percent_Identity=38.3157894736842, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1790167, Length=212, Percent_Identity=29.7169811320755, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=37.4741200828157, Blast_Score=305, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6323958, Length=497, Percent_Identity=33.1991951710262, Blast_Score=246, Evalue=5e-66,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=41.8181818181818, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24659598, Length=492, Percent_Identity=40.8536585365854, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24659604, Length=471, Percent_Identity=41.1889596602972, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54437; Mature: 54306

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLYARQSLPPCRWNAMLTTHPFDDDKLREECGVFGIHGADSASAVVALGLHALQHRGQ
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCC
EAAGITAFDGKEFHTHRAMGHVAGNFDRDEIIRQLDGASAVGHVRYSTTGETALRNVQPL
CCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
FADLSTGGFAIAHNGNISNAAALRKLLVRRGSIFQSTSDTEVIIHLVATSSYRSLLDRFI
HHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECHHHHHHHHHHH
DALKQVEGAYSLICLTAEGMIGCRDPLGIRPLVIGKLGDAHILASETVALDVVGAEFLRS
HHHHHHCCCEEEEEEECCCCEECCCCCCCCCEEEECCCCCEEEECCCEEEEEHHHHHHHC
VEPGELIIIRDGQLTSHRPFAEQSARPCIFEYVYFSRPDSIVDGTSVYSVRKAIGAELAR
CCCCCEEEEECCCCCCCCCCHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
ENPVDADLVIPVPDSGTPAAIGYAQESGIPFELGIIRSHYVGRTFIQPGDKVRHLGVKLK
CCCCCCEEEEECCCCCCCCEEEEHHCCCCCEEEEEHHHHHCCCCCCCCCCCEECCCEEEE
HNANRALIAGQRIVLIDDSIVRGTTSLKIVQMMRDAGAAEVHMRIASPPTSHSCFYGVDT
ECCCEEEEECCEEEEECCCHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCC
PERAKLLAAQMTIGQMANFINADSLAFLTIDGLYRALGEADRKQDAPQYCDACFTGDYPT
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
TLTDFDEHGLEDQFSLLAERVV
CCCCCHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PSLYARQSLPPCRWNAMLTTHPFDDDKLREECGVFGIHGADSASAVVALGLHALQHRGQ
CCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCC
EAAGITAFDGKEFHTHRAMGHVAGNFDRDEIIRQLDGASAVGHVRYSTTGETALRNVQPL
CCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
FADLSTGGFAIAHNGNISNAAALRKLLVRRGSIFQSTSDTEVIIHLVATSSYRSLLDRFI
HHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECHHHHHHHHHHH
DALKQVEGAYSLICLTAEGMIGCRDPLGIRPLVIGKLGDAHILASETVALDVVGAEFLRS
HHHHHHCCCEEEEEEECCCCEECCCCCCCCCEEEECCCCCEEEECCCEEEEEHHHHHHHC
VEPGELIIIRDGQLTSHRPFAEQSARPCIFEYVYFSRPDSIVDGTSVYSVRKAIGAELAR
CCCCCEEEEECCCCCCCCCCHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
ENPVDADLVIPVPDSGTPAAIGYAQESGIPFELGIIRSHYVGRTFIQPGDKVRHLGVKLK
CCCCCCEEEEECCCCCCCCEEEEHHCCCCCEEEEEHHHHHCCCCCCCCCCCEECCCEEEE
HNANRALIAGQRIVLIDDSIVRGTTSLKIVQMMRDAGAAEVHMRIASPPTSHSCFYGVDT
ECCCEEEEECCEEEEECCCHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCC
PERAKLLAAQMTIGQMANFINADSLAFLTIDGLYRALGEADRKQDAPQYCDACFTGDYPT
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
TLTDFDEHGLEDQFSLLAERVV
CCCCCHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA