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The map label for this gene is ilvI [H]

Identifier: 103486955

GI number: 103486955

Start: 1528868

End: 1530613

Strand: Direct

Name: ilvI [H]

Synonym: Sala_1470

Alternate gene names: 103486955

Gene position: 1528868-1530613 (Clockwise)

Preceding gene: 103486954

Following gene: 103486956

Centisome position: 45.7

GC content: 64.95

Gene sequence:

>1746_bases
ATGAGCGGTGCCGACATGGTGGTTCAGGCGCTGGTCGACCTTGGCGTCGATACAGTGTTCGGCTATCCGGGCGGCGCGGT
CCTTCCCATCTATGACGCGCTCTACAAGCACCCGACGATCAAGCATATTCTCGTCCGTCATGAACAGGCGGCGACGCATG
CGGCGGAGGGTTATGCGCGCTCGACCGGCAAGCCGGGCGTCGTACTGGTCACCTCGGGCCCCGGCGCGACGAACGCCGTC
ACTGGCATCACCGACGCCTTGCTCGATTCGATTCCGATGGTCGTGCTCACCGGCCAGGTTCCGACGACGCTGATCGGCAC
CGACGCCTTTCAGGAATGCGACACGGTCGGCATCACGCGCCATTGCACCAAGCATAATTATCTGGTGATGGATCCCGACC
GCCTCGGCCCCGTCATGCACGAGGCGTTCCATATCGCGACGCACGGCCGCCCCGGCCCGGTGGTGATCGACATTCCGAAA
AATGTTCAGGTCGCGACCGGCACCTATTCGGTGCCCGAGTTCATCCAGCACAACAGCTATCGCCCGCAAGTCGAACCCGA
CGCCGACGCGATCGGTCAGGCGATCGACATGATCGCTGCGGCCGAACGTCCGGTCTTCTACACCGGCGGCGGCGTGATCA
ACGCCGGCCCCGATGCGAGCGCTGCCTTGCGCCAGCTCGTCGGCCTGACCGGCGCGCCGATCACCTCGACCTTGATGGGG
CTCGGCGCCTTCCCCGCTGACGATCCCAAATGGCTCGGCATGCTCGGTATGCACGGCACCTATGAAGCCAATATGGCGAT
GAACCGCGCCGACCTGATCGTCGCGGTCGGCGCGCGCTTCGACGACCGCGTGACCGGCCGCCTCGACGCCTTTTCGCCCG
ATTCCAAAAAGATCCATATCGACATTGATCGCAGCTCGATCAACAAGATCGTCCCCGTCGACCTCGCGATCGTCGGCGAT
GCCGGGCGCGCGCTCGCCGCGCTGATCGCCGCGTGGCAGGACAGGGGCCACAAGGCGCGCGACCTCGGCGAATGGTGGCG
CCGCGTCGACGGCTGGCGCGCCACGCGCTGCCTCGACTTCCCCGAAAAAAAGGAGCCCGACGCCGACATCATGCCGCAGC
GCGCGGTGAAGGCGCTGTTCGACGCGACGCGCGGGCGCGATCCGATCATCACCACCGAAGTCGGGCAGCACCAGATGTGG
GCGGCGCAGCATTTCGGCTTCTCCGCCCCGAACCGCTGGCTGACCAGCGGCGGGCTCGGCACGATGGGTTATGGCTTCCC
CGCCGCGGTCGGCGCGCAGATCGCGCACCCGAACCGCCTCGTCATCTGCATCGCGGGCGAAGCCAGCCTTCAGATGAACA
TTCAGGAAATGGGCACGGTCAGCCAGTACCGCCTGCCGGTGAAGATATTCATCCTCAACAATGAATGGATGGGCATGGTC
CGCCAGTGGCAGGAACTGACCTATGAAAGCCGCTATTCGAACAGCTATTCGGACAGCCTGCCCGATTTTGTGAAGCTCGC
CGAAGCCTATGGCTGGACCGGATTACGGATCGACACGCTCGGCGAACTCGAAGGCGGCATCGCCCGGATGATCGAGACTC
CGGGCCCGGTGATCGTCGACTGCCGCGTCGCCCAGCTCGCCAACTGCTTCCCGATGATCCCGAGCGGAGCGGCGCACACC
GACATGATCCTCCAGCCGAACGACATCACCGGCACGATGGACGACGAAGCGAAAGCGCTGGTGTGA

Upstream 100 bases:

>100_bases
CAGTGTTGACAAGATAGAATCATGCAACTATTGCCCGCAACCCTGTTGCAGCGCACAAGCGCTGCGTTTTTGTGTGCAAA
GGAGTTTCGTCGTGACGGAA

Downstream 100 bases:

>100_bases
TGGCGGCGAGCAACGCGATCCTCCCCCATCGGGGGAGGGGGATCATGCGAAGCATGGTGGAGGGGCACGCGGTGTCAAGC
GCGGCGGATGCGGGCCCGTA

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MSGADMVVQALVDLGVDTVFGYPGGAVLPIYDALYKHPTIKHILVRHEQAATHAAEGYARSTGKPGVVLVTSGPGATNAV
TGITDALLDSIPMVVLTGQVPTTLIGTDAFQECDTVGITRHCTKHNYLVMDPDRLGPVMHEAFHIATHGRPGPVVIDIPK
NVQVATGTYSVPEFIQHNSYRPQVEPDADAIGQAIDMIAAAERPVFYTGGGVINAGPDASAALRQLVGLTGAPITSTLMG
LGAFPADDPKWLGMLGMHGTYEANMAMNRADLIVAVGARFDDRVTGRLDAFSPDSKKIHIDIDRSSINKIVPVDLAIVGD
AGRALAALIAAWQDRGHKARDLGEWWRRVDGWRATRCLDFPEKKEPDADIMPQRAVKALFDATRGRDPIITTEVGQHQMW
AAQHFGFSAPNRWLTSGGLGTMGYGFPAAVGAQIAHPNRLVICIAGEASLQMNIQEMGTVSQYRLPVKIFILNNEWMGMV
RQWQELTYESRYSNSYSDSLPDFVKLAEAYGWTGLRIDTLGELEGGIARMIETPGPVIVDCRVAQLANCFPMIPSGAAHT
DMILQPNDITGTMDDEAKALV

Sequences:

>Translated_581_residues
MSGADMVVQALVDLGVDTVFGYPGGAVLPIYDALYKHPTIKHILVRHEQAATHAAEGYARSTGKPGVVLVTSGPGATNAV
TGITDALLDSIPMVVLTGQVPTTLIGTDAFQECDTVGITRHCTKHNYLVMDPDRLGPVMHEAFHIATHGRPGPVVIDIPK
NVQVATGTYSVPEFIQHNSYRPQVEPDADAIGQAIDMIAAAERPVFYTGGGVINAGPDASAALRQLVGLTGAPITSTLMG
LGAFPADDPKWLGMLGMHGTYEANMAMNRADLIVAVGARFDDRVTGRLDAFSPDSKKIHIDIDRSSINKIVPVDLAIVGD
AGRALAALIAAWQDRGHKARDLGEWWRRVDGWRATRCLDFPEKKEPDADIMPQRAVKALFDATRGRDPIITTEVGQHQMW
AAQHFGFSAPNRWLTSGGLGTMGYGFPAAVGAQIAHPNRLVICIAGEASLQMNIQEMGTVSQYRLPVKIFILNNEWMGMV
RQWQELTYESRYSNSYSDSLPDFVKLAEAYGWTGLRIDTLGELEGGIARMIETPGPVIVDCRVAQLANCFPMIPSGAAHT
DMILQPNDITGTMDDEAKALV
>Mature_580_residues
SGADMVVQALVDLGVDTVFGYPGGAVLPIYDALYKHPTIKHILVRHEQAATHAAEGYARSTGKPGVVLVTSGPGATNAVT
GITDALLDSIPMVVLTGQVPTTLIGTDAFQECDTVGITRHCTKHNYLVMDPDRLGPVMHEAFHIATHGRPGPVVIDIPKN
VQVATGTYSVPEFIQHNSYRPQVEPDADAIGQAIDMIAAAERPVFYTGGGVINAGPDASAALRQLVGLTGAPITSTLMGL
GAFPADDPKWLGMLGMHGTYEANMAMNRADLIVAVGARFDDRVTGRLDAFSPDSKKIHIDIDRSSINKIVPVDLAIVGDA
GRALAALIAAWQDRGHKARDLGEWWRRVDGWRATRCLDFPEKKEPDADIMPQRAVKALFDATRGRDPIITTEVGQHQMWA
AQHFGFSAPNRWLTSGGLGTMGYGFPAAVGAQIAHPNRLVICIAGEASLQMNIQEMGTVSQYRLPVKIFILNNEWMGMVR
QWQELTYESRYSNSYSDSLPDFVKLAEAYGWTGLRIDTLGELEGGIARMIETPGPVIVDCRVAQLANCFPMIPSGAAHTD
MILQPNDITGTMDDEAKALV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=24.9557522123894, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI21361361, Length=560, Percent_Identity=25.3571428571429, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI87081685, Length=577, Percent_Identity=49.0467937608319, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=44.3262411347518, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1786717, Length=597, Percent_Identity=33.1658291457286, Blast_Score=305, Evalue=7e-84,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=28.2331511839709, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI1788716, Length=552, Percent_Identity=25, Blast_Score=154, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=584, Percent_Identity=26.541095890411, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=584, Percent_Identity=26.541095890411, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=523, Percent_Identity=26.9598470363289, Blast_Score=129, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=581, Percent_Identity=45.6110154905336, Blast_Score=501, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=26.0869565217391, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320123, Length=588, Percent_Identity=21.4285714285714, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=494, Percent_Identity=22.6720647773279, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19922626, Length=506, Percent_Identity=26.6798418972332, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62668; Mature: 62537

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGADMVVQALVDLGVDTVFGYPGGAVLPIYDALYKHPTIKHILVRHEQAATHAAEGYAR
CCCHHHHHHHHHHCCCHHEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHH
STGKPGVVLVTSGPGATNAVTGITDALLDSIPMVVLTGQVPTTLIGTDAFQECDTVGITR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEECCHHHHHHCCCCCCH
HCTKHNYLVMDPDRLGPVMHEAFHIATHGRPGPVVIDIPKNVQVATGTYSVPEFIQHNSY
HHCCCCCEEECHHHHCHHHHHHEEEEECCCCCCEEEECCCCCEEEECCCCCHHHHHCCCC
RPQVEPDADAIGQAIDMIAAAERPVFYTGGGVINAGPDASAALRQLVGLTGAPITSTLMG
CCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHCCCCCCHHHHHHH
LGAFPADDPKWLGMLGMHGTYEANMAMNRADLIVAVGARFDDRVTGRLDAFSPDSKKIHI
CCCCCCCCCCHHEEECCCCCEECCEECCCCCEEEEECCCCCCCCCCCEECCCCCCCEEEE
DIDRSSINKIVPVDLAIVGDAGRALAALIAAWQDRGHKARDLGEWWRRVDGWRATRCLDF
EECHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCC
PEKKEPDADIMPQRAVKALFDATRGRDPIITTEVGQHQMWAAQHFGFSAPNRWLTSGGLG
CCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEECCCCC
TMGYGFPAAVGAQIAHPNRLVICIAGEASLQMNIQEMGTVSQYRLPVKIFILNNEWMGMV
CCCCCCHHHHCCEECCCCEEEEEEECCCCEEEEHHHHCCHHHEECCEEEEEECCHHHHHH
RQWQELTYESRYSNSYSDSLPDFVKLAEAYGWTGLRIDTLGELEGGIARMIETPGPVIVD
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHCCHHHHHHCCCCCEEEE
CRVAQLANCFPMIPSGAAHTDMILQPNDITGTMDDEAKALV
EEHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
SGADMVVQALVDLGVDTVFGYPGGAVLPIYDALYKHPTIKHILVRHEQAATHAAEGYAR
CCHHHHHHHHHHCCCHHEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHH
STGKPGVVLVTSGPGATNAVTGITDALLDSIPMVVLTGQVPTTLIGTDAFQECDTVGITR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEECCHHHHHHCCCCCCH
HCTKHNYLVMDPDRLGPVMHEAFHIATHGRPGPVVIDIPKNVQVATGTYSVPEFIQHNSY
HHCCCCCEEECHHHHCHHHHHHEEEEECCCCCCEEEECCCCCEEEECCCCCHHHHHCCCC
RPQVEPDADAIGQAIDMIAAAERPVFYTGGGVINAGPDASAALRQLVGLTGAPITSTLMG
CCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHCCCCCCHHHHHHH
LGAFPADDPKWLGMLGMHGTYEANMAMNRADLIVAVGARFDDRVTGRLDAFSPDSKKIHI
CCCCCCCCCCHHEEECCCCCEECCEECCCCCEEEEECCCCCCCCCCCEECCCCCCCEEEE
DIDRSSINKIVPVDLAIVGDAGRALAALIAAWQDRGHKARDLGEWWRRVDGWRATRCLDF
EECHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCC
PEKKEPDADIMPQRAVKALFDATRGRDPIITTEVGQHQMWAAQHFGFSAPNRWLTSGGLG
CCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEECCCCC
TMGYGFPAAVGAQIAHPNRLVICIAGEASLQMNIQEMGTVSQYRLPVKIFILNNEWMGMV
CCCCCCHHHHCCEECCCCEEEEEEECCCCEEEEHHHHCCHHHEECCEEEEEECCHHHHHH
RQWQELTYESRYSNSYSDSLPDFVKLAEAYGWTGLRIDTLGELEGGIARMIETPGPVIVD
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHCCHHHHHHCCCCCEEEE
CRVAQLANCFPMIPSGAAHTDMILQPNDITGTMDDEAKALV
EEHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]