Click here to switch to the map view.

The map label for this gene is miaA

Identifier: 103486954

GI number: 103486954

Start: 1527864

End: 1528784

Strand: Direct

Name: miaA

Synonym: Sala_1469

Alternate gene names: 103486954

Gene position: 1527864-1528784 (Clockwise)

Preceding gene: 103486952

Following gene: 103486955

Centisome position: 45.67

GC content: 68.4

Gene sequence:

>921_bases
GTGGCACTTATCGCCGGACCCACCGCCAGCGGCAAGAGCGCTTTGGCGGTTGCGCTCGCAAAAGCGCTGGGGAACGGCAC
GGTCGTCAACGCCGATGCGAGTCAGGTTTACGCCGACCTCGCCATCCTGTCGGCGCGTCCCACGGACGACGAGATGCAGG
GCGTGCCGCACCGGCTCTTCGGTCATGTCGATGGATCGCAAGCGCACAATGCCGCGCGCTGGGCGGACGAAGCTCAAGGC
GTCATCGCACAGGCGCACGCGGCGGGCCGGGTGCCGATCCTCGTCGGCGGCACCGGCCTTTATCTGCGCACCTTGCTCTA
TGGCATCGCCCCAGTCCCCGAAATCGACCCGCACATCCGCGAAGCGGTCCGCGCACTCCCGGTCGCCCAGGCTCATGCCG
CGCTTGCCGAAGCCGACCCCGCCGCCGCCGCGCGCCTTGGCCCCGCCGACACCACGCGCGTCGCGCGCGCGCTTGAGGTT
GTGCGTTCGACCGGCCGCACGCTGGCCAGTTGGCAGCAGGCGCGCGAAGGCGGCATCGCCGACCGTGTCGCGCTCGCCCC
GCTGGTCCTGTTGCCGCCGCGCGACTGGCTGCGGGACCGCTGCGATGTGCGGCTGGTCGAGATGTTCGATGGCGGCGCCA
TCGACGAAGTCGAAGCGCTGCTCGCCCGCGGCCTCGACCCCGACCTTCCCGTCATGCGCGCAATCGGTGTCCCGCAGATC
GCGGCTTTCCTCGATGGCACGGCGAGCCGCGACGAGGCGCTGGCGGCCGCGCAGGCGGCGACGCGCCAATATGCCAAACG
CCAGTTCACCTGGTTCCGCCATCAGCCCCCGGCGAGCTGGCCGCGCCACATTGAGTCATTAAGCATCGATATTATCGACA
ATTTAGCAATAAAGTTACGTGAGACAGTGTTGACAAGATAG

Upstream 100 bases:

>100_bases
CGCTTCGCGAACCACCTCGTCGGTCAGCTTTCCGGCTGCTATCAGCGTCGCGACGAACATGGCATCTCCTTCTTTCTCGA
CCGCCAACGCGCGGCTGCCC

Downstream 100 bases:

>100_bases
AATCATGCAACTATTGCCCGCAACCCTGTTGCAGCGCACAAGCGCTGCGTTTTTGTGTGCAAAGGAGTTTCGTCGTGACG
GAAATGAGCGGTGCCGACAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MALIAGPTASGKSALAVALAKALGNGTVVNADASQVYADLAILSARPTDDEMQGVPHRLFGHVDGSQAHNAARWADEAQG
VIAQAHAAGRVPILVGGTGLYLRTLLYGIAPVPEIDPHIREAVRALPVAQAHAALAEADPAAAARLGPADTTRVARALEV
VRSTGRTLASWQQAREGGIADRVALAPLVLLPPRDWLRDRCDVRLVEMFDGGAIDEVEALLARGLDPDLPVMRAIGVPQI
AAFLDGTASRDEALAAAQAATRQYAKRQFTWFRHQPPASWPRHIESLSIDIIDNLAIKLRETVLTR

Sequences:

>Translated_306_residues
MALIAGPTASGKSALAVALAKALGNGTVVNADASQVYADLAILSARPTDDEMQGVPHRLFGHVDGSQAHNAARWADEAQG
VIAQAHAAGRVPILVGGTGLYLRTLLYGIAPVPEIDPHIREAVRALPVAQAHAALAEADPAAAARLGPADTTRVARALEV
VRSTGRTLASWQQAREGGIADRVALAPLVLLPPRDWLRDRCDVRLVEMFDGGAIDEVEALLARGLDPDLPVMRAIGVPQI
AAFLDGTASRDEALAAAQAATRQYAKRQFTWFRHQPPASWPRHIESLSIDIIDNLAIKLRETVLTR
>Mature_305_residues
ALIAGPTASGKSALAVALAKALGNGTVVNADASQVYADLAILSARPTDDEMQGVPHRLFGHVDGSQAHNAARWADEAQGV
IAQAHAAGRVPILVGGTGLYLRTLLYGIAPVPEIDPHIREAVRALPVAQAHAALAEADPAAAARLGPADTTRVARALEVV
RSTGRTLASWQQAREGGIADRVALAPLVLLPPRDWLRDRCDVRLVEMFDGGAIDEVEALLARGLDPDLPVMRAIGVPQIA
AFLDGTASRDEALAAAQAATRQYAKRQFTWFRHQPPASWPRHIESLSIDIIDNLAIKLRETVLTR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=24.9190938511327, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1790613, Length=286, Percent_Identity=39.8601398601399, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25144712, Length=302, Percent_Identity=24.1721854304636, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324848, Length=287, Percent_Identity=30.3135888501742, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24649830, Length=352, Percent_Identity=26.1363636363636, Blast_Score=104, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SPHAL (Q1GT40)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616515.1
- ProteinModelPortal:   Q1GT40
- SMR:   Q1GT40
- GeneID:   4081280
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1469
- NMPDR:   fig|317655.9.peg.1402
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   SALA317655:SALA_1469-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 32502; Mature: 32371

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIAGPTASGKSALAVALAKALGNGTVVNADASQVYADLAILSARPTDDEMQGVPHRLF
CCEECCCCCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCHHHHCCCHHHH
GHVDGSQAHNAARWADEAQGVIAQAHAAGRVPILVGGTGLYLRTLLYGIAPVPEIDPHIR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHH
EAVRALPVAQAHAALAEADPAAAARLGPADTTRVARALEVVRSTGRTLASWQQAREGGIA
HHHHHCCHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCH
DRVALAPLVLLPPRDWLRDRCDVRLVEMFDGGAIDEVEALLARGLDPDLPVMRAIGVPQI
HHHHHCCEEEECCHHHHHHHCCEEEEEHHCCCCHHHHHHHHHCCCCCCCHHHHHCCCHHH
AAFLDGTASRDEALAAAQAATRQYAKRQFTWFRHQPPASWPRHIESLSIDIIDNLAIKLR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ETVLTR
HHHHCC
>Mature Secondary Structure 
ALIAGPTASGKSALAVALAKALGNGTVVNADASQVYADLAILSARPTDDEMQGVPHRLF
CEECCCCCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCHHHHCCCHHHH
GHVDGSQAHNAARWADEAQGVIAQAHAAGRVPILVGGTGLYLRTLLYGIAPVPEIDPHIR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHH
EAVRALPVAQAHAALAEADPAAAARLGPADTTRVARALEVVRSTGRTLASWQQAREGGIA
HHHHHCCHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCH
DRVALAPLVLLPPRDWLRDRCDVRLVEMFDGGAIDEVEALLARGLDPDLPVMRAIGVPQI
HHHHHCCEEEECCHHHHHHHCCEEEEEHHCCCCHHHHHHHHHCCCCCCCHHHHHCCCHHH
AAFLDGTASRDEALAAAQAATRQYAKRQFTWFRHQPPASWPRHIESLSIDIIDNLAIKLR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ETVLTR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA