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The map label for this gene is pyrC

Identifier: 103486913

GI number: 103486913

Start: 1477649

End: 1478671

Strand: Direct

Name: pyrC

Synonym: Sala_1427

Alternate gene names: 103486913

Gene position: 1477649-1478671 (Clockwise)

Preceding gene: 103486909

Following gene: 103486916

Centisome position: 44.17

GC content: 67.64

Gene sequence:

>1023_bases
ATGACCGACCGCCTGACGATCCGCCGCCCCGACGACTGGCACGTCCATCTGCGCGACGGCGCGATGCTTGCCCATGTCGC
GCCCTATACCGCGCGCCAGTTTGCGCGCGCGATCGTCATGCCCAATCTGTCGCCGCCGGTGACGACCGCCGAAGAAGGCC
GCGCCTATCGCGACCGCATCACCGCCGCGGTGCCCGCGGACCTCGACTTCACCCCGCTGATCGTCGCCTATCTGACCGAC
GCCACCGACGCGGACGAGATCGCGCGCGGCCATGCCGAGGGCGTGTTCACCGCCGCCAAGCTCTATCCCGCGCACGCCAC
CACCGGCAGCGCGCACGGCGTCACCGACGTCGCGAACATCATGGGTGTGCTCGAGCGGATGCAGGACATCGGCATGCCGC
TGCTCATCCACGGCGAAGTCACCGACCATGACGTCGACATTTTCGACCGCGAGGCGGTGTTCATCGAGCGCACGCTTGAA
CCGCTGGTGCGCGCGCTGCCCGGCCTCAAGATCGTTTTCGAGCATATCACGACGGCGGAGGCCGCGCAGTTTGTCGCCGA
CGCGCCCGCCAATGTCGCCGCGACGATCACGCCGCAGCATCTCCACATCAACCGCAACGCGATGCTTGTCGGCGGCATCC
GCCCGCACGCCTATTGCCTGCCCGTCGCGAAGCGCGAGCATCACCGGCTCGCGGTGCGCGCCGCCGCGACATCGGGCTCG
CCCAAATTCTTCCTCGGCACCGACAGCGCGCCGCACGCGGTGCATATGAAGGAAGCGTCGTGCGGCTGCGCAGGCATTTT
CAACGCGCCCTTTGCGCTCGAAAGCTATGCGATGGCGTTCGATCAGGATGGCGCGCTGGCCAATTTCGAGGGTTTCGCCA
GCGAGCATGGTCCGCGCTTCTATGGCCTGCCGCTCAACGAGGGCACGGTGACGTTGGAACGCGCCGAAATCACCGTCCCC
GACCGGATCGGCGACGTCGTGCCCTTCCACGCGGGCGAAACCATCGGCTGGCGGCTGGTCTGA

Upstream 100 bases:

>100_bases
CAGACGGATGAGCGCGCGGTCGCGAAGGGTGGTAATGGCCATGCCGTTAATCTAGGGACGCCGGAGCCAAAGCCAAGCCC
ACTTACGACGAGTCCTTTCG

Downstream 100 bases:

>100_bases
TCATCCCCCCCGCGATGACGGACGACCGTGTCATACGGCGACAATCCGCTCGGCGCGCGTCTTGCGCCACATGTCGAAAC
TGAACACCGCAATGCTGATC

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTDRLTIRRPDDWHVHLRDGAMLAHVAPYTARQFARAIVMPNLSPPVTTAEEGRAYRDRITAAVPADLDFTPLIVAYLTD
ATDADEIARGHAEGVFTAAKLYPAHATTGSAHGVTDVANIMGVLERMQDIGMPLLIHGEVTDHDVDIFDREAVFIERTLE
PLVRALPGLKIVFEHITTAEAAQFVADAPANVAATITPQHLHINRNAMLVGGIRPHAYCLPVAKREHHRLAVRAAATSGS
PKFFLGTDSAPHAVHMKEASCGCAGIFNAPFALESYAMAFDQDGALANFEGFASEHGPRFYGLPLNEGTVTLERAEITVP
DRIGDVVPFHAGETIGWRLV

Sequences:

>Translated_340_residues
MTDRLTIRRPDDWHVHLRDGAMLAHVAPYTARQFARAIVMPNLSPPVTTAEEGRAYRDRITAAVPADLDFTPLIVAYLTD
ATDADEIARGHAEGVFTAAKLYPAHATTGSAHGVTDVANIMGVLERMQDIGMPLLIHGEVTDHDVDIFDREAVFIERTLE
PLVRALPGLKIVFEHITTAEAAQFVADAPANVAATITPQHLHINRNAMLVGGIRPHAYCLPVAKREHHRLAVRAAATSGS
PKFFLGTDSAPHAVHMKEASCGCAGIFNAPFALESYAMAFDQDGALANFEGFASEHGPRFYGLPLNEGTVTLERAEITVP
DRIGDVVPFHAGETIGWRLV
>Mature_339_residues
TDRLTIRRPDDWHVHLRDGAMLAHVAPYTARQFARAIVMPNLSPPVTTAEEGRAYRDRITAAVPADLDFTPLIVAYLTDA
TDADEIARGHAEGVFTAAKLYPAHATTGSAHGVTDVANIMGVLERMQDIGMPLLIHGEVTDHDVDIFDREAVFIERTLEP
LVRALPGLKIVFEHITTAEAAQFVADAPANVAATITPQHLHINRNAMLVGGIRPHAYCLPVAKREHHRLAVRAAATSGSP
KFFLGTDSAPHAVHMKEASCGCAGIFNAPFALESYAMAFDQDGALANFEGFASEHGPRFYGLPLNEGTVTLERAEITVPD
RIGDVVPFHAGETIGWRLV

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=337, Percent_Identity=62.0178041543027, Blast_Score=424, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6323452, Length=355, Percent_Identity=33.2394366197183, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_SPHAL (Q1GT81)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616474.1
- ProteinModelPortal:   Q1GT81
- SMR:   Q1GT81
- GeneID:   4081744
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1427
- NMPDR:   fig|317655.9.peg.1359
- HOGENOM:   HBG628648
- OMA:   KESACGC
- ProtClustDB:   PRK05451
- BioCyc:   SALA317655:SALA_1427-MONOMER
- BRENDA:   3.5.2.3
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 36843; Mature: 36712

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRLTIRRPDDWHVHLRDGAMLAHVAPYTARQFARAIVMPNLSPPVTTAEEGRAYRDRI
CCCCEEEECCCCCEEEEECCEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHH
TAAVPADLDFTPLIVAYLTDATDADEIARGHAEGVFTAAKLYPAHATTGSAHGVTDVANI
CEECCCCCCCHHEEEEEECCCCCHHHHHCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
MGVLERMQDIGMPLLIHGEVTDHDVDIFDREAVFIERTLEPLVRALPGLKIVFEHITTAE
HHHHHHHHHCCCCEEEECCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AAQFVADAPANVAATITPQHLHINRNAMLVGGIRPHAYCLPVAKREHHRLAVRAAATSGS
HHHHHHCCCCCEEEEECCCEEEECCCEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCC
PKFFLGTDSAPHAVHMKEASCGCAGIFNAPFALESYAMAFDQDGALANFEGFASEHGPRF
CEEEEECCCCCCEEEEECCCCCEEEECCCCHHHHHHHHEECCCCCEECCCCHHHCCCCEE
YGLPLNEGTVTLERAEITVPDRIGDVVPFHAGETIGWRLV
EEEECCCCEEEEEEEEEECCHHHCCCCCCCCCCCCEEEEC
>Mature Secondary Structure 
TDRLTIRRPDDWHVHLRDGAMLAHVAPYTARQFARAIVMPNLSPPVTTAEEGRAYRDRI
CCCEEEECCCCCEEEEECCEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHH
TAAVPADLDFTPLIVAYLTDATDADEIARGHAEGVFTAAKLYPAHATTGSAHGVTDVANI
CEECCCCCCCHHEEEEEECCCCCHHHHHCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
MGVLERMQDIGMPLLIHGEVTDHDVDIFDREAVFIERTLEPLVRALPGLKIVFEHITTAE
HHHHHHHHHCCCCEEEECCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AAQFVADAPANVAATITPQHLHINRNAMLVGGIRPHAYCLPVAKREHHRLAVRAAATSGS
HHHHHHCCCCCEEEEECCCEEEECCCEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCC
PKFFLGTDSAPHAVHMKEASCGCAGIFNAPFALESYAMAFDQDGALANFEGFASEHGPRF
CEEEEECCCCCCEEEEECCCCCEEEECCCCHHHHHHHHEECCCCCEECCCCHHHCCCCEE
YGLPLNEGTVTLERAEITVPDRIGDVVPFHAGETIGWRLV
EEEECCCCEEEEEEEEEECCHHHCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA