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The map label for this gene is gph [H]

Identifier: 103486857

GI number: 103486857

Start: 1426862

End: 1427530

Strand: Direct

Name: gph [H]

Synonym: Sala_1371

Alternate gene names: 103486857

Gene position: 1426862-1427530 (Clockwise)

Preceding gene: 103486850

Following gene: 103486865

Centisome position: 42.65

GC content: 67.71

Gene sequence:

>669_bases
ATGAACGGATTTCCTTTTGCGATCGTCGGCTTCGACCTCGACGGCACGCTCGTCGACACGCTCGGCGACCTTGCCGCGGC
GGTCAATCATGCGCTGGCGCAGATGGACCGCGCACCCCTGAGCGAAGCGGCGGTGCGGCCGATGATCGGGCGCGGGGCCA
GACATATGCTCGAACAGGGACTCATCGCGACCGGCGGCGCACCCGACGGTGCGGTCGAGCGGCTCTATCCCGAACTCCTC
CGCTTTTATGAAGCGCATATCGCGACGCAAAGCCGTCCCTTTGCGGGCGCCGTAACCGCGCTCGACCGGTTGGATGCGCT
CGGTGTCCGCGCTGCAGTCGTGACGAACAAGGTCGAGCGGCTGGCGCGCCAGCTGCTCGGCGAACTGAGGCTGGCCGACC
GGATGACAGCGATCATCGGCGGCGACACGCTGGGACCGGGCGGCGCCAAGCCCTCCCCCGCCCCGATCCGCGCGATGATC
GAGCGATGCGGCGGCGGCCGCGCGGCCTTCGTCGGCGACAGTATTTTCGACGTTCAGGCGGCGCGCGGCGCTGACGTCCT
CAGCGTCGCAGTCGGCTTCGGCTTTCTCGATGGCCCGGTCGAAACGCTCGGCGCGGATCATGTCATCGGCCATTTCGACG
AACTGGTGCCACTGCTCGGTGAGCTATAG

Upstream 100 bases:

>100_bases
TTGCCCGCGGCAAGGACGATGGCGGCGATCGGGTTGTTCATGCGCGCTGCCATGCCAGCAAATCATTGCGGTTTCCAGCA
CATCGCGCCATGCGCGCGGG

Downstream 100 bases:

>100_bases
CCCTCCCCTGAAGGGGCGGGCTCTAGGAGGGCTTCCGCCACTTCGTCCAAAGATGCGTCGCCAGCATCAGGGGCGGCATC
CGCAGCCAGTGCGATCGGAT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNGFPFAIVGFDLDGTLVDTLGDLAAAVNHALAQMDRAPLSEAAVRPMIGRGARHMLEQGLIATGGAPDGAVERLYPELL
RFYEAHIATQSRPFAGAVTALDRLDALGVRAAVVTNKVERLARQLLGELRLADRMTAIIGGDTLGPGGAKPSPAPIRAMI
ERCGGGRAAFVGDSIFDVQAARGADVLSVAVGFGFLDGPVETLGADHVIGHFDELVPLLGEL

Sequences:

>Translated_222_residues
MNGFPFAIVGFDLDGTLVDTLGDLAAAVNHALAQMDRAPLSEAAVRPMIGRGARHMLEQGLIATGGAPDGAVERLYPELL
RFYEAHIATQSRPFAGAVTALDRLDALGVRAAVVTNKVERLARQLLGELRLADRMTAIIGGDTLGPGGAKPSPAPIRAMI
ERCGGGRAAFVGDSIFDVQAARGADVLSVAVGFGFLDGPVETLGADHVIGHFDELVPLLGEL
>Mature_222_residues
MNGFPFAIVGFDLDGTLVDTLGDLAAAVNHALAQMDRAPLSEAAVRPMIGRGARHMLEQGLIATGGAPDGAVERLYPELL
RFYEAHIATQSRPFAGAVTALDRLDALGVRAAVVTNKVERLARQLLGELRLADRMTAIIGGDTLGPGGAKPSPAPIRAMI
ERCGGGRAAFVGDSIFDVQAARGADVLSVAVGFGFLDGPVETLGADHVIGHFDELVPLLGEL

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=233, Percent_Identity=33.9055793991416, Blast_Score=81, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23102; Mature: 23102

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGFPFAIVGFDLDGTLVDTLGDLAAAVNHALAQMDRAPLSEAAVRPMIGRGARHMLEQG
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LIATGGAPDGAVERLYPELLRFYEAHIATQSRPFAGAVTALDRLDALGVRAAVVTNKVER
CEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARQLLGELRLADRMTAIIGGDTLGPGGAKPSPAPIRAMIERCGGGRAAFVGDSIFDVQA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHHH
ARGADVLSVAVGFGFLDGPVETLGADHVIGHFDELVPLLGEL
HCCCHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGFPFAIVGFDLDGTLVDTLGDLAAAVNHALAQMDRAPLSEAAVRPMIGRGARHMLEQG
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LIATGGAPDGAVERLYPELLRFYEAHIATQSRPFAGAVTALDRLDALGVRAAVVTNKVER
CEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARQLLGELRLADRMTAIIGGDTLGPGGAKPSPAPIRAMIERCGGGRAAFVGDSIFDVQA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHHH
ARGADVLSVAVGFGFLDGPVETLGADHVIGHFDELVPLLGEL
HCCCHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]