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The map label for this gene is trmL [H]

Identifier: 103486832

GI number: 103486832

Start: 1406180

End: 1406638

Strand: Direct

Name: trmL [H]

Synonym: Sala_1346

Alternate gene names: 103486832

Gene position: 1406180-1406638 (Clockwise)

Preceding gene: 103486826

Following gene: 103486833

Centisome position: 42.04

GC content: 67.76

Gene sequence:

>459_bases
ATGGAAATCGCATTGTTCCAACCCGACATCGCCGGCAATGTCGGCACGATTCTGCGCACCGCCTCCTGCTTCGGCGTGCC
CGCGCATATCATCGAGCCGTGCGGCTTTCCGTTCGGCGACGCGGCGCTCCGGCGCGCCGGCATGGACTATGCCGCGCGCG
CCGACGTGCGGCGCCACGCCGATTGGGACGGCTTTTCCGGCTGGTCGCACAGCACGGCGGGTCGGCTGGTTCTCCTGACC
ACCGCAGGCGCCACACGGCTGCCTGACTTCGCGTTCGCGCACGGCGACATATTGCTGTTCGGATCGGAAAGTGCAGGCGT
TCCGCCGCACGTTCACGCCGCCGCCGCCGAACGGGTCGCGATTCCGATGCAGCCCGGCTTTCGATGCTTGAATGTCGCGG
TCGCGGCTGGCATCGCGCTCGCCGAGGCGCTCAGGCAGACGAAAGGCTTTCCGGCATGA

Upstream 100 bases:

>100_bases
GTCCGGGCTTGCCAACAGGCAATTTCCGCTTCCGCCACCCGGCCGCCCCGCCGCAGCCAGACCTCTCGATTCGTGGCGCC
TTCGCCTTTATAGCGGCGAC

Downstream 100 bases:

>100_bases
TCCCGCTCGATCCGCAGCAACAGAAGGCGCGCAACTGGTTCGAATCGCTTCGCGACCGCATCTGCGCCGAGTTCGAGGCG
ATCGAGGCCGAAGCGGGCAG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MEIALFQPDIAGNVGTILRTASCFGVPAHIIEPCGFPFGDAALRRAGMDYAARADVRRHADWDGFSGWSHSTAGRLVLLT
TAGATRLPDFAFAHGDILLFGSESAGVPPHVHAAAAERVAIPMQPGFRCLNVAVAAGIALAEALRQTKGFPA

Sequences:

>Translated_152_residues
MEIALFQPDIAGNVGTILRTASCFGVPAHIIEPCGFPFGDAALRRAGMDYAARADVRRHADWDGFSGWSHSTAGRLVLLT
TAGATRLPDFAFAHGDILLFGSESAGVPPHVHAAAAERVAIPMQPGFRCLNVAVAAGIALAEALRQTKGFPA
>Mature_152_residues
MEIALFQPDIAGNVGTILRTASCFGVPAHIIEPCGFPFGDAALRRAGMDYAARADVRRHADWDGFSGWSHSTAGRLVLLT
TAGATRLPDFAFAHGDILLFGSESAGVPPHVHAAAAERVAIPMQPGFRCLNVAVAAGIALAEALRQTKGFPA

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=37.9084967320261, Blast_Score=102, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 15907; Mature: 15907

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIALFQPDIAGNVGTILRTASCFGVPAHIIEPCGFPFGDAALRRAGMDYAARADVRRHA
CEEEEECCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHCC
DWDGFSGWSHSTAGRLVLLTTAGATRLPDFAFAHGDILLFGSESAGVPPHVHAAAAERVA
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCEEEECCCCCCCCCCCHHHHHCEEE
IPMQPGFRCLNVAVAAGIALAEALRQTKGFPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEIALFQPDIAGNVGTILRTASCFGVPAHIIEPCGFPFGDAALRRAGMDYAARADVRRHA
CEEEEECCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHCC
DWDGFSGWSHSTAGRLVLLTTAGATRLPDFAFAHGDILLFGSESAGVPPHVHAAAAERVA
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCEEEECCCCCCCCCCCHHHHHCEEE
IPMQPGFRCLNVAVAAGIALAEALRQTKGFPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA