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The map label for this gene is holB [H]

Identifier: 103486744

GI number: 103486744

Start: 1310442

End: 1311422

Strand: Direct

Name: holB [H]

Synonym: Sala_1258

Alternate gene names: 103486744

Gene position: 1310442-1311422 (Clockwise)

Preceding gene: 103486743

Following gene: 103486745

Centisome position: 39.17

GC content: 66.87

Gene sequence:

>981_bases
ATGATCGGCCATCAGGAGGCCGAAAAGGCGTTTCTGGACGGCTGGCAGGGCGGCCGCCTGCACCATGCCTGGCTGCTCGC
CGGGCCACAGGGGATGGGGAAGGGGGACTTTGCCGAGCGCGCGGCGCGCTTTCTCGTGACCCATGGCGCAGGTGGCGGGC
GCGCGGCGGTAGCACTTGACGATCGAGGCGACGATGCCGCCGCACGGCTCGTCGATGCGGGCAATCACCCGGAGATCATC
CGGCTGACGCGCCAGCCCAAGGACAAGACCAAGGAGCTTGCGCGTAACATCACCATCGACCAGGTGCGCCAGGTGATCCG
CCGTCTGCATCTGTCGCTGTCGCTCGGCGACTGGCGCGTCATCATCGTCGATGCCGTCGACGACCTGGAAAGCGATGGTG
CCAATGCACTGCTCAAGACGCTCGAGGAACCGCCCGCCAGGACGCTGTTCCTGCTGGTCAGCCATTCGCCGGGGCGGCTG
CTCCCGACGATTCGTTCACGCTGTCGAATCCTTCGTTTTCAACCGATTGACCGTGACGTCATGGTGTCATGGCTCCACGA
TCTGCGACCAATGCTCGACATGCCCGAGGTGCGCGCGATCGTCGCCGCGTCGGGCGGGGTGCCCGGCAAGGCGCTGGCGT
TGATCGACAGTGATGTCGCGTCGATGCAAAAGAAGCTGCTGGCCATCGCCGCCTCGGGCGACCCCGAGAACCGGCTGCGC
GAGGCGCTCGCGCGCGAGGTCGGCGGCGCGAGCAATCGCGCGCGGCTTGAACTGGTGATCGACATCGTTCCCGGATTGCT
GGCAGCAATGGCGCGCGAGCGTCCGATCACCGAGATCGCGCCTGTGCTGGCCCAGTGGGACCGTGTGCAGCGCACGGTGC
GCGATGCTGTCCGGGGCTCCTACGATGGCACGATGGTCGGTTTCGAGATCGGGAACTGCCTCGCGGAGCTTGCGCTGCGA
TCGGCACAGGCGGCACGCTGA

Upstream 100 bases:

>100_bases
TGGCCGCGACCGAGCCGCAGCGCTGGCGGTTGATCGACGCCGACCTGCCCGCCGCCGACGTCACCGCGGCCATGCTTGCG
GAGCTGATCGAATGGCTGCC

Downstream 100 bases:

>100_bases
CGCTTTCCCTCGCGCGCGGCAGCGGCTAAGGCGCGCGCATGTCCCAACAGAGCGCTCCCTTCTATATCACCACCGCGATC
AGCTATCCCAACGGCCGCCC

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MIGHQEAEKAFLDGWQGGRLHHAWLLAGPQGMGKGDFAERAARFLVTHGAGGGRAAVALDDRGDDAAARLVDAGNHPEII
RLTRQPKDKTKELARNITIDQVRQVIRRLHLSLSLGDWRVIIVDAVDDLESDGANALLKTLEEPPARTLFLLVSHSPGRL
LPTIRSRCRILRFQPIDRDVMVSWLHDLRPMLDMPEVRAIVAASGGVPGKALALIDSDVASMQKKLLAIAASGDPENRLR
EALAREVGGASNRARLELVIDIVPGLLAAMARERPITEIAPVLAQWDRVQRTVRDAVRGSYDGTMVGFEIGNCLAELALR
SAQAAR

Sequences:

>Translated_326_residues
MIGHQEAEKAFLDGWQGGRLHHAWLLAGPQGMGKGDFAERAARFLVTHGAGGGRAAVALDDRGDDAAARLVDAGNHPEII
RLTRQPKDKTKELARNITIDQVRQVIRRLHLSLSLGDWRVIIVDAVDDLESDGANALLKTLEEPPARTLFLLVSHSPGRL
LPTIRSRCRILRFQPIDRDVMVSWLHDLRPMLDMPEVRAIVAASGGVPGKALALIDSDVASMQKKLLAIAASGDPENRLR
EALAREVGGASNRARLELVIDIVPGLLAAMARERPITEIAPVLAQWDRVQRTVRDAVRGSYDGTMVGFEIGNCLAELALR
SAQAAR
>Mature_326_residues
MIGHQEAEKAFLDGWQGGRLHHAWLLAGPQGMGKGDFAERAARFLVTHGAGGGRAAVALDDRGDDAAARLVDAGNHPEII
RLTRQPKDKTKELARNITIDQVRQVIRRLHLSLSLGDWRVIIVDAVDDLESDGANALLKTLEEPPARTLFLLVSHSPGRL
LPTIRSRCRILRFQPIDRDVMVSWLHDLRPMLDMPEVRAIVAASGGVPGKALALIDSDVASMQKKLLAIAASGDPENRLR
EALAREVGGASNRARLELVIDIVPGLLAAMARERPITEIAPVLAQWDRVQRTVRDAVRGSYDGTMVGFEIGNCLAELALR
SAQAAR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=231, Percent_Identity=32.9004329004329, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786676, Length=228, Percent_Identity=28.5087719298246, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35503; Mature: 35503

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGHQEAEKAFLDGWQGGRLHHAWLLAGPQGMGKGDFAERAARFLVTHGAGGGRAAVALD
CCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHEEECCCCCEEEEEEC
DRGDDAAARLVDAGNHPEIIRLTRQPKDKTKELARNITIDQVRQVIRRLHLSLSLGDWRV
CCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHEEECCEEE
IIVDAVDDLESDGANALLKTLEEPPARTLFLLVSHSPGRLLPTIRSRCRILRFQPIDRDV
EEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHEEECCCCCHHH
MVSWLHDLRPMLDMPEVRAIVAASGGVPGKALALIDSDVASMQKKLLAIAASGDPENRLR
HHHHHHHHHHHHCCCHHHEEEEECCCCCCCEEEEHHHHHHHHHHHHEEEECCCCHHHHHH
EALAREVGGASNRARLELVIDIVPGLLAAMARERPITEIAPVLAQWDRVQRTVRDAVRGS
HHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
YDGTMVGFEIGNCLAELALRSAQAAR
CCCEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIGHQEAEKAFLDGWQGGRLHHAWLLAGPQGMGKGDFAERAARFLVTHGAGGGRAAVALD
CCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHEEECCCCCEEEEEEC
DRGDDAAARLVDAGNHPEIIRLTRQPKDKTKELARNITIDQVRQVIRRLHLSLSLGDWRV
CCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHEEECCEEE
IIVDAVDDLESDGANALLKTLEEPPARTLFLLVSHSPGRLLPTIRSRCRILRFQPIDRDV
EEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHEEECCCCCHHH
MVSWLHDLRPMLDMPEVRAIVAASGGVPGKALALIDSDVASMQKKLLAIAASGDPENRLR
HHHHHHHHHHHHCCCHHHEEEEECCCCCCCEEEEHHHHHHHHHHHHEEEECCCCHHHHHH
EALAREVGGASNRARLELVIDIVPGLLAAMARERPITEIAPVLAQWDRVQRTVRDAVRGS
HHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
YDGTMVGFEIGNCLAELALRSAQAAR
CCCEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]