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The map label for this gene is metG [H]

Identifier: 103486745

GI number: 103486745

Start: 1311461

End: 1313044

Strand: Direct

Name: metG [H]

Synonym: Sala_1259

Alternate gene names: 103486745

Gene position: 1311461-1313044 (Clockwise)

Preceding gene: 103486744

Following gene: 103486746

Centisome position: 39.2

GC content: 61.36

Gene sequence:

>1584_bases
ATGTCCCAACAGAGCGCTCCCTTCTATATCACCACCGCGATCAGCTATCCCAACGGCCGCCCGCACATCGGCCACGCCTA
TGAAGCGATCGCCACCGACGTCATGGCGCGGTTCCAGCGCGCGCGCGGCCGCAACGTCCGGCTGGTGACCGGCACCGACG
AACATGGGCTGAAGATGTTTCAGACCGCGCGTGACCAGGGGCGCGCGACGATCGATCTGGCAAATGAAATGTCCGGATAT
TTCCGGGAGATGTATGCCAAGTTGAATATCAGCTATGATATGTTCATGCGCACGTCCGATCCGGCGCACCACGCCGCCTC
GCAGGCGATCTGGAAGGCGATGGAAGCCAATGGCGACCTTTATCTCGACCGCTATGAGGGTTGGTATTCGGTTCGCGACG
AAGCCTTTTACGATGAGAGCGAGCTGACCGACGGGGGGGAGGGCATCCGCCTTTCGCCGCAGGGCACGCCGGTCGAATGG
ACCGTCGAGGAAAGCTGGTTCTTCCGCCTGTCGAACTATCAGGATCGGCTGCTCGCGCTCTATCGCGACAATCCCGACTT
CATCCGCCCCGAAAGCCGCCGCAACGAGGTGCTGCGCTTCGTCGAGGGCGGTTTGAAGGATTTGAGCGTCTCGCGCACCA
GTTTCGACTGGGGGGTTCCGGTGCCGGGATCGCCGGGGCACGTCATGTATGTCTGGGTTGATGCGCTGACGACCTATTTG
TCGGGGCTGGGCTATCCCGACCCGGCGAGCGACTTCGGCACATTCTGGCCCGCGAACATTCATATGATCGGCAAGGATAT
CGTTCGTTTTCATACTGTTTACTGGCCGGCCTTTCTGATGAGTGCCAATCTGCCGCTGCCTCGCCAGGTGTTCGGCCACG
GCTTTCTGCTCAACCGCGGCGAAAAAATGTCGAAGTCGCTCGGCAATGTCGTCGATCCGATGGCGCTCGCCGAGCGGTTC
GGCGTCGATGCGCTGCGCTATTATCTGCTGCGCGAGGTCAGCTTCGGACAGGACGGCAGCTATTCGGCCGAAGCGATCGT
CCGCACCGCCAACGCCGACCTCGCCAACAGCTTTGGCAATCTGGCGCAGCGCAGCTTGTCGATGATTTTCAAAAACTTGG
GCGGTGAACTTTCGGCTGACTATACTCCGGCACAGGATGATATTGATCTGTTCCACTTGGTCAATCTCAGCGCAAAAGAC
CTATTACCTCGGGCGTTCGAACAACTGGCCTTTTCGGTCGGGATCGAGGAGTGGCTGCGCGCTGTCTTCGCCTGCAACCA
ATATGTCGATGCGCAGGCCCCTTGGGCGTTGCGCAAGAGCGATCCCGAACGCATGCGCGTCGTTCTGATGACGCTGTTCC
AGGCCGTGCGCACGCTCGCCATTGCCATTCGGCCCGTCGTGCCGACGGCGGCCGACAATCTGCTCGATCAGATGGGCATT
GCCCCCGACGCGCGTGATTTTGCGGCGCTGGCCGACGCCGACTGGTTCGCCAGACTGGCGGCGAGCGGTTTCACGGTCGG
GCAGCCCGTGCCGATCTTTCCGCGCCTCGACCTGCCCGAAGAGGAGGGGGAAGGGGAGGCGTGA

Upstream 100 bases:

>100_bases
GTTTCGAGATCGGGAACTGCCTCGCGGAGCTTGCGCTGCGATCGGCACAGGCGGCACGCTGACGCTTTCCCTCGCGCGCG
GCAGCGGCTAAGGCGCGCGC

Downstream 100 bases:

>100_bases
TGCTCGTCGATTCGCACTGTCACCTCAATTACAAGGGCCTCGCCGAGCAGCAGGACGAGGTGCTGGCGCGCGCACGAGAG
CGGGGCGTGACCGCGATGCT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MSQQSAPFYITTAISYPNGRPHIGHAYEAIATDVMARFQRARGRNVRLVTGTDEHGLKMFQTARDQGRATIDLANEMSGY
FREMYAKLNISYDMFMRTSDPAHHAASQAIWKAMEANGDLYLDRYEGWYSVRDEAFYDESELTDGGEGIRLSPQGTPVEW
TVEESWFFRLSNYQDRLLALYRDNPDFIRPESRRNEVLRFVEGGLKDLSVSRTSFDWGVPVPGSPGHVMYVWVDALTTYL
SGLGYPDPASDFGTFWPANIHMIGKDIVRFHTVYWPAFLMSANLPLPRQVFGHGFLLNRGEKMSKSLGNVVDPMALAERF
GVDALRYYLLREVSFGQDGSYSAEAIVRTANADLANSFGNLAQRSLSMIFKNLGGELSADYTPAQDDIDLFHLVNLSAKD
LLPRAFEQLAFSVGIEEWLRAVFACNQYVDAQAPWALRKSDPERMRVVLMTLFQAVRTLAIAIRPVVPTAADNLLDQMGI
APDARDFAALADADWFARLAASGFTVGQPVPIFPRLDLPEEEGEGEA

Sequences:

>Translated_527_residues
MSQQSAPFYITTAISYPNGRPHIGHAYEAIATDVMARFQRARGRNVRLVTGTDEHGLKMFQTARDQGRATIDLANEMSGY
FREMYAKLNISYDMFMRTSDPAHHAASQAIWKAMEANGDLYLDRYEGWYSVRDEAFYDESELTDGGEGIRLSPQGTPVEW
TVEESWFFRLSNYQDRLLALYRDNPDFIRPESRRNEVLRFVEGGLKDLSVSRTSFDWGVPVPGSPGHVMYVWVDALTTYL
SGLGYPDPASDFGTFWPANIHMIGKDIVRFHTVYWPAFLMSANLPLPRQVFGHGFLLNRGEKMSKSLGNVVDPMALAERF
GVDALRYYLLREVSFGQDGSYSAEAIVRTANADLANSFGNLAQRSLSMIFKNLGGELSADYTPAQDDIDLFHLVNLSAKD
LLPRAFEQLAFSVGIEEWLRAVFACNQYVDAQAPWALRKSDPERMRVVLMTLFQAVRTLAIAIRPVVPTAADNLLDQMGI
APDARDFAALADADWFARLAASGFTVGQPVPIFPRLDLPEEEGEGEA
>Mature_526_residues
SQQSAPFYITTAISYPNGRPHIGHAYEAIATDVMARFQRARGRNVRLVTGTDEHGLKMFQTARDQGRATIDLANEMSGYF
REMYAKLNISYDMFMRTSDPAHHAASQAIWKAMEANGDLYLDRYEGWYSVRDEAFYDESELTDGGEGIRLSPQGTPVEWT
VEESWFFRLSNYQDRLLALYRDNPDFIRPESRRNEVLRFVEGGLKDLSVSRTSFDWGVPVPGSPGHVMYVWVDALTTYLS
GLGYPDPASDFGTFWPANIHMIGKDIVRFHTVYWPAFLMSANLPLPRQVFGHGFLLNRGEKMSKSLGNVVDPMALAERFG
VDALRYYLLREVSFGQDGSYSAEAIVRTANADLANSFGNLAQRSLSMIFKNLGGELSADYTPAQDDIDLFHLVNLSAKDL
LPRAFEQLAFSVGIEEWLRAVFACNQYVDAQAPWALRKSDPERMRVVLMTLFQAVRTLAIAIRPVVPTAADNLLDQMGIA
PDARDFAALADADWFARLAASGFTVGQPVPIFPRLDLPEEEGEGEA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=552, Percent_Identity=35.8695652173913, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI14043022, Length=560, Percent_Identity=25.7142857142857, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1788432, Length=509, Percent_Identity=28.8801571709234, Blast_Score=195, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI212645096, Length=492, Percent_Identity=33.5365853658537, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI71992781, Length=385, Percent_Identity=32.2077922077922, Blast_Score=224, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17540794, Length=556, Percent_Identity=25.5395683453237, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321610, Length=557, Percent_Identity=32.3159784560144, Blast_Score=283, Evalue=3e-77,
Organism=Drosophila melanogaster, GI21358483, Length=505, Percent_Identity=35.2475247524752, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24655311, Length=550, Percent_Identity=25.0909090909091, Blast_Score=132, Evalue=6e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 59105; Mature: 58974

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQSAPFYITTAISYPNGRPHIGHAYEAIATDVMARFQRARGRNVRLVTGTDEHGLKMF
CCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
QTARDQGRATIDLANEMSGYFREMYAKLNISYDMFMRTSDPAHHAASQAIWKAMEANGDL
HHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCE
YLDRYEGWYSVRDEAFYDESELTDGGEGIRLSPQGTPVEWTVEESWFFRLSNYQDRLLAL
EEECCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCCEEEEECCCEEEEECCCCCCEEEE
YRDNPDFIRPESRRNEVLRFVEGGLKDLSVSRTSFDWGVPVPGSPGHVMYVWVDALTTYL
ECCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
SGLGYPDPASDFGTFWPANIHMIGKDIVRFHTVYWPAFLMSANLPLPRQVFGHGFLLNRG
HHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEECCC
EKMSKSLGNVVDPMALAERFGVDALRYYLLREVSFGQDGSYSAEAIVRTANADLANSFGN
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCHHHHHHHH
LAQRSLSMIFKNLGGELSADYTPAQDDIDLFHLVNLSAKDLLPRAFEQLAFSVGIEEWLR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHH
AVFACNQYVDAQAPWALRKSDPERMRVVLMTLFQAVRTLAIAIRPVVPTAADNLLDQMGI
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
APDARDFAALADADWFARLAASGFTVGQPVPIFPRLDLPEEEGEGEA
CCCCHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQQSAPFYITTAISYPNGRPHIGHAYEAIATDVMARFQRARGRNVRLVTGTDEHGLKMF
CCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
QTARDQGRATIDLANEMSGYFREMYAKLNISYDMFMRTSDPAHHAASQAIWKAMEANGDL
HHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCE
YLDRYEGWYSVRDEAFYDESELTDGGEGIRLSPQGTPVEWTVEESWFFRLSNYQDRLLAL
EEECCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCCEEEEECCCEEEEECCCCCCEEEE
YRDNPDFIRPESRRNEVLRFVEGGLKDLSVSRTSFDWGVPVPGSPGHVMYVWVDALTTYL
ECCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
SGLGYPDPASDFGTFWPANIHMIGKDIVRFHTVYWPAFLMSANLPLPRQVFGHGFLLNRG
HHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEECCC
EKMSKSLGNVVDPMALAERFGVDALRYYLLREVSFGQDGSYSAEAIVRTANADLANSFGN
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCHHHHHHHH
LAQRSLSMIFKNLGGELSADYTPAQDDIDLFHLVNLSAKDLLPRAFEQLAFSVGIEEWLR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHH
AVFACNQYVDAQAPWALRKSDPERMRVVLMTLFQAVRTLAIAIRPVVPTAADNLLDQMGI
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
APDARDFAALADADWFARLAASGFTVGQPVPIFPRLDLPEEEGEGEA
CCCCHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]