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The map label for this gene is proS

Identifier: 103486717

GI number: 103486717

Start: 1277797

End: 1279380

Strand: Direct

Name: proS

Synonym: Sala_1230

Alternate gene names: 103486717

Gene position: 1277797-1279380 (Clockwise)

Preceding gene: 103486716

Following gene: 103486718

Centisome position: 38.2

GC content: 64.65

Gene sequence:

>1584_bases
TTGCAAAAAAGAAATAGGCGGGACGTCGTTCCGCACCCGAGCATAGAAACAGGACCGATGATCAAACATGCGCTCAGCGT
AACCCGTCAGGCCGACTTCGCGGCCTGGTATCAGGACGTGATTGCCGAGGCCGATCTGGCCGAGGAGTCGGGGGTGCGCG
GCTGCATGGTCATCAAGCCGTGGGGCTATGGCATCTGGGAACGCATCCAGACGGTGATGGACGCGGCGATCAAGGATGCC
GGGGTCCAGAACGCCTATTTCCCGCTGTTCATTCCCTTGAGCTTCTTTGAGAAAGAAGCCGACCATGTCGATGGTTTTGC
AAAGGAAATGGCGGTCGTCACGCACCACCGGCTGATCGCGGACAGCAAGGGGAAACTCGTTCCCGATCCCGAAGCGAAGC
TCGAAGAACCGCTGATCGTCCGCCCGACGTCGGAAACGGTGATCGGCGCCGCGATGAGCCGCTGGGTGCAAAGCTGGCGC
GACCTGCCGCTCAAGGTCAACCAGTGGGCGAATGTCGTGCGCTGGGAAATGCGCACGCGCATGTTCCTGCGCACGAGCGA
GTTTCTGTGGCAGGAGGGCCATACCGCGCACGCCGACAGGGACGATGCGATGGCCGAGACGCTGCGTGCGCTCGAAATGT
ACCGCAGCTTTGCCGAGGATGTGCTGGCCATGCCGGTGATCGCGGGTGAAAAGCCCGAAAATGAGCGGTTTCCGGGCGCG
GTCGCGACCTATTCGATCGAGGCGATGATGCAGGACGGCAAGGCGCTTCAGGCCGGCACGTCGCATTATCTGGGTACCGG
TTTCGCCGAAGCCGCGGGCATTCGCTATCAGGACAGGGACGGCGGGCACAGCCTGTGCCATACGACGAGCTGGGGCACAT
CGACGCGCATGATCGGCGGCGTGATCATGACGCACGGCGACGACGACGGGCTGCGCTGCCCCCCACGCATCGCGCCGCAC
CAGATCGTCATCGTCCCGATGCTGCGCGACAATGGCGAAGACGCCGCGATCCTCGATTATTGCCGCGACCTCGAAAGTCG
GCTCAAGGCGCTCGACGCCTTCGGCGAGCCCGTGCGCGTGCTGCTCGACACCGGCGCGAACAAGGCGCAGACGAAACGCT
GGGGCTGGGTCAAGAAGGGCGCGCCGATCATCGTCGAGGTCGGTCCGCGCGACGTCGCGGGCGGCAATGTCGCGGTGATC
CGGCGCGACCGGCTTTATCAGGAAAGCGGCAAGCTCAATTCGGCATTCGTCGCGAAAGGCGATTTCATCGCCGACGCGGC
GGCCACGCTGGAGGACATCCAGGCGAGCCTCTATGCCGAAGCGCGCGAGCGGCTCGACGCCAATATCCGCCGCGACGTCA
CCGACCTCGCCGCGCATTTCTCGGGTGAGGACAGGTTCGTCGGCTGGGCCGAGGTGCAATGGGCACGCCCGACCGGCGGC
GCGCTCGACCGGATTGTCGAGCAGCTGAAGGCGCTCAAGCTGACGATGCGCAACACCCCGCTCGACGCCGCGCCCGCGGA
CAGCGCGTGCATCTTCACCGGCGAACCCGCGGTCGAGCGCGTGTTGATCGGGCGGACCTATTAA

Upstream 100 bases:

>100_bases
TTCCAGCAGGCCGCGGGCGCTTTCATTCCGGGGGCGGGCAAGGCAAAGGATGCCGAAATCGCGGCGCTGAAGGCCGAAGT
GGCCGAACTCAGGGCCGAGC

Downstream 100 bases:

>100_bases
TTGGCGAAACGGCAACAGCCTGCTGGTCTTCCCAGCCCGGAACAGATCCTGCGTTTCATCGAAGAAAGCAGGGACGCGGT
CGGCAAGCGCGAGATCGCGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MQKRNRRDVVPHPSIETGPMIKHALSVTRQADFAAWYQDVIAEADLAEESGVRGCMVIKPWGYGIWERIQTVMDAAIKDA
GVQNAYFPLFIPLSFFEKEADHVDGFAKEMAVVTHHRLIADSKGKLVPDPEAKLEEPLIVRPTSETVIGAAMSRWVQSWR
DLPLKVNQWANVVRWEMRTRMFLRTSEFLWQEGHTAHADRDDAMAETLRALEMYRSFAEDVLAMPVIAGEKPENERFPGA
VATYSIEAMMQDGKALQAGTSHYLGTGFAEAAGIRYQDRDGGHSLCHTTSWGTSTRMIGGVIMTHGDDDGLRCPPRIAPH
QIVIVPMLRDNGEDAAILDYCRDLESRLKALDAFGEPVRVLLDTGANKAQTKRWGWVKKGAPIIVEVGPRDVAGGNVAVI
RRDRLYQESGKLNSAFVAKGDFIADAAATLEDIQASLYAEARERLDANIRRDVTDLAAHFSGEDRFVGWAEVQWARPTGG
ALDRIVEQLKALKLTMRNTPLDAAPADSACIFTGEPAVERVLIGRTY

Sequences:

>Translated_527_residues
MQKRNRRDVVPHPSIETGPMIKHALSVTRQADFAAWYQDVIAEADLAEESGVRGCMVIKPWGYGIWERIQTVMDAAIKDA
GVQNAYFPLFIPLSFFEKEADHVDGFAKEMAVVTHHRLIADSKGKLVPDPEAKLEEPLIVRPTSETVIGAAMSRWVQSWR
DLPLKVNQWANVVRWEMRTRMFLRTSEFLWQEGHTAHADRDDAMAETLRALEMYRSFAEDVLAMPVIAGEKPENERFPGA
VATYSIEAMMQDGKALQAGTSHYLGTGFAEAAGIRYQDRDGGHSLCHTTSWGTSTRMIGGVIMTHGDDDGLRCPPRIAPH
QIVIVPMLRDNGEDAAILDYCRDLESRLKALDAFGEPVRVLLDTGANKAQTKRWGWVKKGAPIIVEVGPRDVAGGNVAVI
RRDRLYQESGKLNSAFVAKGDFIADAAATLEDIQASLYAEARERLDANIRRDVTDLAAHFSGEDRFVGWAEVQWARPTGG
ALDRIVEQLKALKLTMRNTPLDAAPADSACIFTGEPAVERVLIGRTY
>Mature_527_residues
MQKRNRRDVVPHPSIETGPMIKHALSVTRQADFAAWYQDVIAEADLAEESGVRGCMVIKPWGYGIWERIQTVMDAAIKDA
GVQNAYFPLFIPLSFFEKEADHVDGFAKEMAVVTHHRLIADSKGKLVPDPEAKLEEPLIVRPTSETVIGAAMSRWVQSWR
DLPLKVNQWANVVRWEMRTRMFLRTSEFLWQEGHTAHADRDDAMAETLRALEMYRSFAEDVLAMPVIAGEKPENERFPGA
VATYSIEAMMQDGKALQAGTSHYLGTGFAEAAGIRYQDRDGGHSLCHTTSWGTSTRMIGGVIMTHGDDDGLRCPPRIAPH
QIVIVPMLRDNGEDAAILDYCRDLESRLKALDAFGEPVRVLLDTGANKAQTKRWGWVKKGAPIIVEVGPRDVAGGNVAVI
RRDRLYQESGKLNSAFVAKGDFIADAAATLEDIQASLYAEARERLDANIRRDVTDLAAHFSGEDRFVGWAEVQWARPTGG
ALDRIVEQLKALKLTMRNTPLDAAPADSACIFTGEPAVERVLIGRTY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=524, Percent_Identity=37.5954198473282, Blast_Score=322, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI71991342, Length=425, Percent_Identity=37.6470588235294, Blast_Score=289, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71991346, Length=367, Percent_Identity=40.5994550408719, Blast_Score=283, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6321808, Length=438, Percent_Identity=39.2694063926941, Blast_Score=305, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24649468, Length=435, Percent_Identity=37.4712643678161, Blast_Score=293, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24649466, Length=435, Percent_Identity=37.4712643678161, Blast_Score=292, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYP_SPHAL (Q1GTS7)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616278.1
- ProteinModelPortal:   Q1GTS7
- SMR:   Q1GTS7
- GeneID:   4080454
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1230
- NMPDR:   fig|317655.9.peg.1165
- HOGENOM:   HBG334108
- OMA:   HEAYEDL
- ProtClustDB:   PRK08661
- BioCyc:   SALA317655:SALA_1230-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 58494; Mature: 58494

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKRNRRDVVPHPSIETGPMIKHALSVTRQADFAAWYQDVIAEADLAEESGVRGCMVIKP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
WGYGIWERIQTVMDAAIKDAGVQNAYFPLFIPLSFFEKEADHVDGFAKEMAVVTHHRLIA
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DSKGKLVPDPEAKLEEPLIVRPTSETVIGAAMSRWVQSWRDLPLKVNQWANVVRWEMRTR
CCCCCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHH
MFLRTSEFLWQEGHTAHADRDDAMAETLRALEMYRSFAEDVLAMPVIAGEKPENERFPGA
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
VATYSIEAMMQDGKALQAGTSHYLGTGFAEAAGIRYQDRDGGHSLCHTTSWGTSTRMIGG
HHHHHHHHHHHCCCHHHCCCCHHCCCCHHHHCCCEEECCCCCCCEEEECCCCCCHHHHCE
VIMTHGDDDGLRCPPRIAPHQIVIVPMLRDNGEDAAILDYCRDLESRLKALDAFGEPVRV
EEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
LLDTGANKAQTKRWGWVKKGAPIIVEVGPRDVAGGNVAVIRRDRLYQESGKLNSAFVAKG
HEECCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEHHHHHHHCCCCCCEEEECC
DFIADAAATLEDIQASLYAEARERLDANIRRDVTDLAAHFSGEDRFVGWAEVQWARPTGG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCH
ALDRIVEQLKALKLTMRNTPLDAAPADSACIFTGEPAVERVLIGRTY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHCCCCCC
>Mature Secondary Structure
MQKRNRRDVVPHPSIETGPMIKHALSVTRQADFAAWYQDVIAEADLAEESGVRGCMVIKP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
WGYGIWERIQTVMDAAIKDAGVQNAYFPLFIPLSFFEKEADHVDGFAKEMAVVTHHRLIA
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DSKGKLVPDPEAKLEEPLIVRPTSETVIGAAMSRWVQSWRDLPLKVNQWANVVRWEMRTR
CCCCCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHH
MFLRTSEFLWQEGHTAHADRDDAMAETLRALEMYRSFAEDVLAMPVIAGEKPENERFPGA
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
VATYSIEAMMQDGKALQAGTSHYLGTGFAEAAGIRYQDRDGGHSLCHTTSWGTSTRMIGG
HHHHHHHHHHHCCCHHHCCCCHHCCCCHHHHCCCEEECCCCCCCEEEECCCCCCHHHHCE
VIMTHGDDDGLRCPPRIAPHQIVIVPMLRDNGEDAAILDYCRDLESRLKALDAFGEPVRV
EEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
LLDTGANKAQTKRWGWVKKGAPIIVEVGPRDVAGGNVAVIRRDRLYQESGKLNSAFVAKG
HEECCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEHHHHHHHCCCCCCEEEECC
DFIADAAATLEDIQASLYAEARERLDANIRRDVTDLAAHFSGEDRFVGWAEVQWARPTGG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCH
ALDRIVEQLKALKLTMRNTPLDAAPADSACIFTGEPAVERVLIGRTY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA