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The map label for this gene is dadB [H]

Identifier: 103486714

GI number: 103486714

Start: 1272468

End: 1273514

Strand: Direct

Name: dadB [H]

Synonym: Sala_1227

Alternate gene names: 103486714

Gene position: 1272468-1273514 (Clockwise)

Preceding gene: 103486713

Following gene: 103486716

Centisome position: 38.04

GC content: 68.58

Gene sequence:

>1047_bases
ATGATTGCCATCGCTCCGCCGCTCCGCCTCCGTCTCGATTCCGACGCGCTTATCGCCAACTGGCGCTGGCTCGCGGGGCA
GGGTGGTGCGGCGGCGTGCGGCGCGGCGATCAAGGCCGACGGATATGGGCTGGGCGCGCGCTGGGTGATGGAGCGGCTCG
CTGCCGCGGGTTGCCGCGACTTCTTCGTTGCAACCTGGGCCGAGGCTGCGGCGCTGATGCCGCTGCCGCAGGGCATTTCG
GTGTCGGTGCTTCATGGAGTCGGCGCCGCCGACATGGTTGCCGCGCGCCTGCTCCCCGCGCGCCCGGTGCTGAACAGCGT
CGAACAGGTGCAGCGCTGGCGCGATAGCGGCGAGGGGCGGCCGTGCGACGTGATGGTCGATACGGGAATGAACCGCCTCG
GCCTGCGGGTCGAAGAGGTGATGGGCGGTGCGCTCGACGGGCTGGCGATCGACATATTGCTCAGCCACCTTGCCTCGGCC
GACGAGGATTGCGGCCAGAATGCGGCGCAGCTGGCGAGTTTTCGATCTTTGCGCGAGACGATACGGGCACGGCGATACAG
CCTCGCCAACAGCGCGGGCATCTGTCTTGGCGCCGACTATGCATTCGACCTGACGCGCCCCGGCATCGCGCTCTACGGCG
GCACGCCGCGCCGCGAAGCGCAGGGACATATACGGCAGGTCGTCTATCCCGAAACGCGCGTGCTACAGTTGCGCGAGGTC
CGGGCGGGCGAAAGCGTCGGTTATGGCGCAACATGGACGGCATCCACGGACACCCGGGTGGCGGTGACCAACATGGGCTA
TGCCGATGGCTATCTGCGCTGTCATGCCGGGTCGGGTGGCGCGACCTGGCGGGGCCAGTCGCTGCCGCTGATCGGGCGCG
TGTCGATGGATTTGACGGCGTTCGACGCGAGCAGCACAGAGGGGCTGCGCGAGGACGACTGGCTGGCGATCGAATATGAC
CTGCCGACGCGCGCGGCGGCCAGCGGCCTGTCGCAATATGAGCTGCTGACGGGATTGGGGAGCCGGTTCGGGCGCGGGTG
GACGTAA

Upstream 100 bases:

>100_bases
TCCGGTCGAGGGGAAGTGGGATCGCGCCGGCGGCGCGGCGGCGCCCACGTCCTGACGCGCCCCATTGGCGCGGCGCGCGA
AGCCGTATAGGGGCGGGGGG

Downstream 100 bases:

>100_bases
TAGTAGAGGCGCGTTCACGAAAAAAAGGGGCCGTCTTGCGACGGCCCCCAATTGGTCGGTTAGCCCAGAGTTAGAGCGAA
GCCTGGATTCCCGCGTAGAA

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MIAIAPPLRLRLDSDALIANWRWLAGQGGAAACGAAIKADGYGLGARWVMERLAAAGCRDFFVATWAEAAALMPLPQGIS
VSVLHGVGAADMVAARLLPARPVLNSVEQVQRWRDSGEGRPCDVMVDTGMNRLGLRVEEVMGGALDGLAIDILLSHLASA
DEDCGQNAAQLASFRSLRETIRARRYSLANSAGICLGADYAFDLTRPGIALYGGTPRREAQGHIRQVVYPETRVLQLREV
RAGESVGYGATWTASTDTRVAVTNMGYADGYLRCHAGSGGATWRGQSLPLIGRVSMDLTAFDASSTEGLREDDWLAIEYD
LPTRAAASGLSQYELLTGLGSRFGRGWT

Sequences:

>Translated_348_residues
MIAIAPPLRLRLDSDALIANWRWLAGQGGAAACGAAIKADGYGLGARWVMERLAAAGCRDFFVATWAEAAALMPLPQGIS
VSVLHGVGAADMVAARLLPARPVLNSVEQVQRWRDSGEGRPCDVMVDTGMNRLGLRVEEVMGGALDGLAIDILLSHLASA
DEDCGQNAAQLASFRSLRETIRARRYSLANSAGICLGADYAFDLTRPGIALYGGTPRREAQGHIRQVVYPETRVLQLREV
RAGESVGYGATWTASTDTRVAVTNMGYADGYLRCHAGSGGATWRGQSLPLIGRVSMDLTAFDASSTEGLREDDWLAIEYD
LPTRAAASGLSQYELLTGLGSRFGRGWT
>Mature_348_residues
MIAIAPPLRLRLDSDALIANWRWLAGQGGAAACGAAIKADGYGLGARWVMERLAAAGCRDFFVATWAEAAALMPLPQGIS
VSVLHGVGAADMVAARLLPARPVLNSVEQVQRWRDSGEGRPCDVMVDTGMNRLGLRVEEVMGGALDGLAIDILLSHLASA
DEDCGQNAAQLASFRSLRETIRARRYSLANSAGICLGADYAFDLTRPGIALYGGTPRREAQGHIRQVVYPETRVLQLREV
RAGESVGYGATWTASTDTRVAVTNMGYADGYLRCHAGSGGATWRGQSLPLIGRVSMDLTAFDASSTEGLREDDWLAIEYD
LPTRAAASGLSQYELLTGLGSRFGRGWT

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1790487, Length=359, Percent_Identity=32.3119777158774, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787439, Length=363, Percent_Identity=30.0275482093664, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 37131; Mature: 37131

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIAPPLRLRLDSDALIANWRWLAGQGGAAACGAAIKADGYGLGARWVMERLAAAGCRD
CEEECCCCEEEECCCEEEEEEEEECCCCCCHHHCCEEECCCCCCCHHHHHHHHHHCCHHH
FFVATWAEAAALMPLPQGISVSVLHGVGAADMVAARLLPARPVLNSVEQVQRWRDSGEGR
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
PCDVMVDTGMNRLGLRVEEVMGGALDGLAIDILLSHLASADEDCGQNAAQLASFRSLRET
CEEEEECCCCHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHH
IRARRYSLANSAGICLGADYAFDLTRPGIALYGGTPRREAQGHIRQVVYPETRVLQLREV
HHHHHHHHCCCCCEEEECCCEEECCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHH
RAGESVGYGATWTASTDTRVAVTNMGYADGYLRCHAGSGGATWRGQSLPLIGRVSMDLTA
CCCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEE
FDASSTEGLREDDWLAIEYDLPTRAAASGLSQYELLTGLGSRFGRGWT
ECCCCCCCCCCCCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIAIAPPLRLRLDSDALIANWRWLAGQGGAAACGAAIKADGYGLGARWVMERLAAAGCRD
CEEECCCCEEEECCCEEEEEEEEECCCCCCHHHCCEEECCCCCCCHHHHHHHHHHCCHHH
FFVATWAEAAALMPLPQGISVSVLHGVGAADMVAARLLPARPVLNSVEQVQRWRDSGEGR
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
PCDVMVDTGMNRLGLRVEEVMGGALDGLAIDILLSHLASADEDCGQNAAQLASFRSLRET
CEEEEECCCCHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHH
IRARRYSLANSAGICLGADYAFDLTRPGIALYGGTPRREAQGHIRQVVYPETRVLQLREV
HHHHHHHHCCCCCEEEECCCEEECCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHH
RAGESVGYGATWTASTDTRVAVTNMGYADGYLRCHAGSGGATWRGQSLPLIGRVSMDLTA
CCCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEE
FDASSTEGLREDDWLAIEYDLPTRAAASGLSQYELLTGLGSRFGRGWT
ECCCCCCCCCCCCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]