Click here to switch to the map view.

The map label for this gene is proP [H]

Identifier: 103486713

GI number: 103486713

Start: 1270779

End: 1272422

Strand: Direct

Name: proP [H]

Synonym: Sala_1226

Alternate gene names: 103486713

Gene position: 1270779-1272422 (Clockwise)

Preceding gene: 103486711

Following gene: 103486714

Centisome position: 37.99

GC content: 64.23

Gene sequence:

>1644_bases
ATGACCGATGCAGCCGCCGTCGCCGACCATGGCGCGGTCTATCAGCCGACGCAGAAAGATATCCGCATGGTCATCGCCGC
ATCGTCGGCGGGGACGGTGTTCGAATGGTATGATTTCTTCATCTACGGCACGCTCGCGGCGATCATCGGCAAGGCCTTTT
TCCCAAGCGACAATGCGACCCTCGAAACCCTGCTCGTCTGGGCCGGCTTTGCGGTCGGTTTTGGATTCCGCCCGCTCGGT
GCCGTCCTCTTCGGCTTCCTCGGCGACCGGCTGGGCCGCAAATATACCTTCCTCGTAACCGTTACCCTGATGGGCATCGC
GACTGCGGGGGTCGGGATGATCCCGTCGGCGGCGACGATCGGCGTCGCCGCGCCAATCATCGTCATCCTGCTGCGGATAT
TGCAGGGGCTCGCGCTTGGTGGCGAATATGGCGGCGCGGCCATCTATGTCGCCGAACATTCGCCGCCCGGAAAGCGGGGT
TTCTATACCAGCTTCATCCAGGCGAGCGTCGTCGGCGGCTTCGTGCTCAGCCTGATCGTTGTGCTCGGGTGCAAGGCGCT
GATGCCCGATGCCGTCTGGGAAAGCTGGGGGTGGCGCGTGCCCTTCCTCCTGTCGCTCATCCTGCTCGGTATTTCGCTGT
GGATGCGCCTCAAACTTTCCGAAAGCCCGGTCTTCCAGGCGATGAAGCAGGAGGGCGAACTGGCGCGTAATCCGCTGAAG
GAAAGCTTTACCTATCCGGGCAATCCGCGGCGCATCTTCATTGCGCTGTTCGGCATCGCTGCCGGGCTCACGGTGATCTG
GTACACGGCGATGTTCTCCGGCCTCTCCTTCCTCAAAGGCCCGATGAAGGTCGAGGAGACCGCCGCCGAAATCATCGTCG
GCTGCGCTGCCGCAGTCGGCATGGGCTTTTTCATCTGGGCAGGCCGCCTGTCGGACCGCATCGGGCGGAAAAAGCCGATC
GTGTGGGGCTATGGCGCCACGCTGCTGCTGCTGTTCCCGCTTTTCTGGTGGATGGGAAGCGTCGGCAATCCCGCGCTGGC
CGCTGCGGCCGAAAGGGCGCCGGTGGTCGTCTCCGGCTCGCAATGCAGCTTCGATCCCTTTGCGCAGAGCCAGGCGACCG
CGTGCGGGCGAACGCTGGGTGAACTGACGAAGCTTGGCGTGCCCTATACGGTGGTCGAAACGCCGGGCGGCTTCGACAGC
GTCACCGTCCGGATCGGCGGCCGCGAGGTTGCGGGGGAGGATCCCGCGCTGCTCCGGCCCGCGCTCGAGGCGATGGGTTA
TGACTTTGCCAAACAGGTTCCCGGCGCGGGGGGCATCGCGATCATCTTCCTCGCGCTGCTGGGGCTCAGCGCGCTGTCGG
GTTTCACCTATGGGCCGGTCGCGGCCTTGCTGTCGGAAATGTTCCCGCCACATGTGCGTTATTCGTCGCTGTCGATTCCC
TATCATATCGGCACCGGCTATTTCGGGGGTTTCCTGCCGTTGATCGCCAGCTTCATCATCGCCAAGACGGGCAATGCCTA
TGCCGGGCTCTGGTATACATGGGGCGTCGTGCTCGCGGCGTTTCTGATCACCGCCTTCATGCTGAGGGATCCGGTCGAGG
GGAAGTGGGATCGCGCCGGCGGCGCGGCGGCGCCCACGTCCTGA

Upstream 100 bases:

>100_bases
AAGGGCATCTCGACTTTGGTTTATCCGGAGCGCCTGCAAGGCAGCCGAAGGGCTCGACGCGAACGGGAGGGGACAGGCCG
GGGAGGCCAAGGGAGAGCAT

Downstream 100 bases:

>100_bases
CGCGCCCCATTGGCGCGGCGCGCGAAGCCGTATAGGGGCGGGGGGATGATTGCCATCGCTCCGCCGCTCCGCCTCCGTCT
CGATTCCGACGCGCTTATCG

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTDAAAVADHGAVYQPTQKDIRMVIAASSAGTVFEWYDFFIYGTLAAIIGKAFFPSDNATLETLLVWAGFAVGFGFRPLG
AVLFGFLGDRLGRKYTFLVTVTLMGIATAGVGMIPSAATIGVAAPIIVILLRILQGLALGGEYGGAAIYVAEHSPPGKRG
FYTSFIQASVVGGFVLSLIVVLGCKALMPDAVWESWGWRVPFLLSLILLGISLWMRLKLSESPVFQAMKQEGELARNPLK
ESFTYPGNPRRIFIALFGIAAGLTVIWYTAMFSGLSFLKGPMKVEETAAEIIVGCAAAVGMGFFIWAGRLSDRIGRKKPI
VWGYGATLLLLFPLFWWMGSVGNPALAAAAERAPVVVSGSQCSFDPFAQSQATACGRTLGELTKLGVPYTVVETPGGFDS
VTVRIGGREVAGEDPALLRPALEAMGYDFAKQVPGAGGIAIIFLALLGLSALSGFTYGPVAALLSEMFPPHVRYSSLSIP
YHIGTGYFGGFLPLIASFIIAKTGNAYAGLWYTWGVVLAAFLITAFMLRDPVEGKWDRAGGAAAPTS

Sequences:

>Translated_547_residues
MTDAAAVADHGAVYQPTQKDIRMVIAASSAGTVFEWYDFFIYGTLAAIIGKAFFPSDNATLETLLVWAGFAVGFGFRPLG
AVLFGFLGDRLGRKYTFLVTVTLMGIATAGVGMIPSAATIGVAAPIIVILLRILQGLALGGEYGGAAIYVAEHSPPGKRG
FYTSFIQASVVGGFVLSLIVVLGCKALMPDAVWESWGWRVPFLLSLILLGISLWMRLKLSESPVFQAMKQEGELARNPLK
ESFTYPGNPRRIFIALFGIAAGLTVIWYTAMFSGLSFLKGPMKVEETAAEIIVGCAAAVGMGFFIWAGRLSDRIGRKKPI
VWGYGATLLLLFPLFWWMGSVGNPALAAAAERAPVVVSGSQCSFDPFAQSQATACGRTLGELTKLGVPYTVVETPGGFDS
VTVRIGGREVAGEDPALLRPALEAMGYDFAKQVPGAGGIAIIFLALLGLSALSGFTYGPVAALLSEMFPPHVRYSSLSIP
YHIGTGYFGGFLPLIASFIIAKTGNAYAGLWYTWGVVLAAFLITAFMLRDPVEGKWDRAGGAAAPTS
>Mature_546_residues
TDAAAVADHGAVYQPTQKDIRMVIAASSAGTVFEWYDFFIYGTLAAIIGKAFFPSDNATLETLLVWAGFAVGFGFRPLGA
VLFGFLGDRLGRKYTFLVTVTLMGIATAGVGMIPSAATIGVAAPIIVILLRILQGLALGGEYGGAAIYVAEHSPPGKRGF
YTSFIQASVVGGFVLSLIVVLGCKALMPDAVWESWGWRVPFLLSLILLGISLWMRLKLSESPVFQAMKQEGELARNPLKE
SFTYPGNPRRIFIALFGIAAGLTVIWYTAMFSGLSFLKGPMKVEETAAEIIVGCAAAVGMGFFIWAGRLSDRIGRKKPIV
WGYGATLLLLFPLFWWMGSVGNPALAAAAERAPVVVSGSQCSFDPFAQSQATACGRTLGELTKLGVPYTVVETPGGFDSV
TVRIGGREVAGEDPALLRPALEAMGYDFAKQVPGAGGIAIIFLALLGLSALSGFTYGPVAALLSEMFPPHVRYSSLSIPY
HIGTGYFGGFLPLIASFIIAKTGNAYAGLWYTWGVVLAAFLITAFMLRDPVEGKWDRAGGAAAPTS

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=328, Percent_Identity=36.280487804878, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1788292, Length=315, Percent_Identity=40, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789941, Length=356, Percent_Identity=37.6404494382022, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788942, Length=346, Percent_Identity=33.2369942196532, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI87082231, Length=195, Percent_Identity=26.1538461538462, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082404, Length=340, Percent_Identity=25.5882352941176, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 58005; Mature: 57873

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAAAVADHGAVYQPTQKDIRMVIAASSAGTVFEWYDFFIYGTLAAIIGKAFFPSDNAT
CCCCHHHCCCCCCCCCCHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LETLLVWAGFAVGFGFRPLGAVLFGFLGDRLGRKYTFLVTVTLMGIATAGVGMIPSAATI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCHHHH
GVAAPIIVILLRILQGLALGGEYGGAAIYVAEHSPPGKRGFYTSFIQASVVGGFVLSLIV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
VLGCKALMPDAVWESWGWRVPFLLSLILLGISLWMRLKLSESPVFQAMKQEGELARNPLK
HHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHCCHH
ESFTYPGNPRRIFIALFGIAAGLTVIWYTAMFSGLSFLKGPMKVEETAAEIIVGCAAAVG
HCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MGFFIWAGRLSDRIGRKKPIVWGYGATLLLLFPLFWWMGSVGNPALAAAAERAPVVVSGS
HHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEECC
QCSFDPFAQSQATACGRTLGELTKLGVPYTVVETPGGFDSVTVRIGGREVAGEDPALLRP
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCEECCCCCHHHHHH
ALEAMGYDFAKQVPGAGGIAIIFLALLGLSALSGFTYGPVAALLSEMFPPHVRYSSLSIP
HHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCC
YHIGTGYFGGFLPLIASFIIAKTGNAYAGLWYTWGVVLAAFLITAFMLRDPVEGKWDRAG
EEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GAAAPTS
CCCCCCC
>Mature Secondary Structure 
TDAAAVADHGAVYQPTQKDIRMVIAASSAGTVFEWYDFFIYGTLAAIIGKAFFPSDNAT
CCCHHHCCCCCCCCCCHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCH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CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]