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The map label for this gene is bioB

Identifier: 103486709

GI number: 103486709

Start: 1265868

End: 1266857

Strand: Direct

Name: bioB

Synonym: Sala_1222

Alternate gene names: 103486709

Gene position: 1265868-1266857 (Clockwise)

Preceding gene: 103486708

Following gene: 103486710

Centisome position: 37.84

GC content: 66.36

Gene sequence:

>990_bases
ATGCGCACCGACTGGACCCGCGAGGAAATCGCCGCCCTGTTCGACCTGCCGTTCGACGAACTGATGTGGGAGGCGCAAGG
CGTCCACCGCCGCCATCACGCGCGCGGCGAAATCCAGCTCTGCACCCTGCTCAGCATCAAGACCGGCGGCTGCGTCGAGG
ATTGCGGCTATTGCTCGCAGTCGGCGCACGCCGACACCGGCCTGAAAGCGACCAAGCTGATGGACGTGCGCGCGGTGCTG
CAAGCCGCGGCGCAGGCGAAGGATGCGGGGTCGAAGCGCTTCTGCATGGGCGCCGCGTGGCGCAACCCCAAGGACCGCGA
CATGCCCGCGATCGTCGAGATGATCGAAGGCGTGCGCGCGATGGGCATGGAAACCTGCATGACGCTGGGGATGCTGAGCA
AGGAACAGGCGCAGGTGCTGGGCATGGCGGGGCTCGATTATTACAACCACAATATCGACACCTCGCCGGAACATTACGGC
AATGTCATCACGACGCGGAGCTTTCAGGACCGTCTCGATACGCTGGAGGAAGTGCGCGCGGCGGGGATCAACGTGTGCAG
CGGCGGCATCGTCGGCATGGGCGAGACGCGCGCCGACCGGGTGGGTTTCATCCACACGCTTGCGACCCTGCCCGAACATC
CGGGCAGCGTACCGATCAACGCACTGGTGCCGGTGAAGGGGACCGTGCTCGGCGACATGCTGGCCGACACCCCGCTCGCC
AAGATCGACGATATCGAGTTCGTCCGCACCGTCGCGGTGGCGCGCATCACCATGCCGAAGTCGATGGTCCGCCTGTCGGC
GGGCCGTGAAAGCATGTCGGAGGCGACGCAGGCGCTCTGCTTCATGGCGGGCGCGAACAGCATCTTCACCGGCGACAAGC
TGCTGACGACCGCGAACGCGGGTGACAATGCCGACGCGGCGCTGTTCGCGAAGCTGGGGCTGCGGCCGATGGAGAGCGAA
GAGCCGATGCGGATGGAGGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
CTCTACCCCGCTCGTGTCGAGCGAAGTCGAGACACCCATCAACCGTAAGCCCAGCCCGAGGGGCATCTCGACTTCGCTCG
ATGCGAACGGGGAAGGGGAG

Downstream 100 bases:

>100_bases
AATATTGGCGGCCAGCCATCGTGACGTTGCTGATCGTCGCGCTTCTTTACTGGCTGATGTTCGCGTCGGGCATGTTCATG
CACGGCGATCCGATTGAATG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MRTDWTREEIAALFDLPFDELMWEAQGVHRRHHARGEIQLCTLLSIKTGGCVEDCGYCSQSAHADTGLKATKLMDVRAVL
QAAAQAKDAGSKRFCMGAAWRNPKDRDMPAIVEMIEGVRAMGMETCMTLGMLSKEQAQVLGMAGLDYYNHNIDTSPEHYG
NVITTRSFQDRLDTLEEVRAAGINVCSGGIVGMGETRADRVGFIHTLATLPEHPGSVPINALVPVKGTVLGDMLADTPLA
KIDDIEFVRTVAVARITMPKSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTTANAGDNADAALFAKLGLRPMESE
EPMRMEAAE

Sequences:

>Translated_329_residues
MRTDWTREEIAALFDLPFDELMWEAQGVHRRHHARGEIQLCTLLSIKTGGCVEDCGYCSQSAHADTGLKATKLMDVRAVL
QAAAQAKDAGSKRFCMGAAWRNPKDRDMPAIVEMIEGVRAMGMETCMTLGMLSKEQAQVLGMAGLDYYNHNIDTSPEHYG
NVITTRSFQDRLDTLEEVRAAGINVCSGGIVGMGETRADRVGFIHTLATLPEHPGSVPINALVPVKGTVLGDMLADTPLA
KIDDIEFVRTVAVARITMPKSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTTANAGDNADAALFAKLGLRPMESE
EPMRMEAAE
>Mature_329_residues
MRTDWTREEIAALFDLPFDELMWEAQGVHRRHHARGEIQLCTLLSIKTGGCVEDCGYCSQSAHADTGLKATKLMDVRAVL
QAAAQAKDAGSKRFCMGAAWRNPKDRDMPAIVEMIEGVRAMGMETCMTLGMLSKEQAQVLGMAGLDYYNHNIDTSPEHYG
NVITTRSFQDRLDTLEEVRAAGINVCSGGIVGMGETRADRVGFIHTLATLPEHPGSVPINALVPVKGTVLGDMLADTPLA
KIDDIEFVRTVAVARITMPKSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTTANAGDNADAALFAKLGLRPMESE
EPMRMEAAE

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=334, Percent_Identity=58.9820359281437, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321725, Length=317, Percent_Identity=52.0504731861199, Blast_Score=350, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_SPHAL (Q1GTT5)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616270.1
- ProteinModelPortal:   Q1GTT5
- SMR:   Q1GTT5
- GeneID:   4080446
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1222
- NMPDR:   fig|317655.9.peg.1156
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2391555
- BioCyc:   SALA317655:SALA_1222-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35642; Mature: 35642

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTDWTREEIAALFDLPFDELMWEAQGVHRRHHARGEIQLCTLLSIKTGGCVEDCGYCSQ
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHCCCCC
SAHADTGLKATKLMDVRAVLQAAAQAKDAGSKRFCMGAAWRNPKDRDMPAIVEMIEGVRA
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHH
MGMETCMTLGMLSKEQAQVLGMAGLDYYNHNIDTSPEHYGNVITTRSFQDRLDTLEEVRA
HHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHH
AGINVCSGGIVGMGETRADRVGFIHTLATLPEHPGSVPINALVPVKGTVLGDMLADTPLA
CCCEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCCCCC
KIDDIEFVRTVAVARITMPKSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTTANA
HHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECCCEEEEECCC
GDNADAALFAKLGLRPMESEEPMRMEAAE
CCCCCHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTDWTREEIAALFDLPFDELMWEAQGVHRRHHARGEIQLCTLLSIKTGGCVEDCGYCSQ
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHCCCCC
SAHADTGLKATKLMDVRAVLQAAAQAKDAGSKRFCMGAAWRNPKDRDMPAIVEMIEGVRA
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHH
MGMETCMTLGMLSKEQAQVLGMAGLDYYNHNIDTSPEHYGNVITTRSFQDRLDTLEEVRA
HHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHH
AGINVCSGGIVGMGETRADRVGFIHTLATLPEHPGSVPINALVPVKGTVLGDMLADTPLA
CCCEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCCCCC
KIDDIEFVRTVAVARITMPKSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTTANA
HHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECCCEEEEECCC
GDNADAALFAKLGLRPMESEEPMRMEAAE
CCCCCHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA