Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 103486557

GI number: 103486557

Start: 1100283

End: 1101599

Strand: Reverse

Name: purB [H]

Synonym: Sala_1068

Alternate gene names: 103486557

Gene position: 1101599-1100283 (Counterclockwise)

Preceding gene: 103486559

Following gene: 103486556

Centisome position: 32.93

GC content: 65.3

Gene sequence:

>1317_bases
ATGGTTCCGCGTTATGCACGGCCGGAAATGACGGCGATCTGGTCGGCGGAGAATCGCTTCTCCATCTGGTTCGAGATCGA
AGCCCACGCGACCGACGCGCTCGCCGAACTCGGCACCGTGCCCCCATCGGCGGCAAAGGCGCTGTGGGACTGGTGGGCGA
CCAGGCCTGCGATCGACGTCGCTGCAATCGACGCGATCGAGGCCGTGACCAAGCATGACGTCATCGCCTTCCTTACCTGG
GTTGCCGAAAATGTCGGCGAGGAAGCGCGCTTCATGCACCAGGGCATGACCAGCTCCGACGTGCTCGACACCTGCCTCGC
GGTCCAGCTGGCGCAGGCCGCCGACATATTGCTCGCGGACCTCGAGGCGCTGCTCGCCGCGATCAAGCGCCGCGCGTTCG
AGCACAAGCTCACCCCGACGATCGGGCGCAGCCACGGCATCCACGCCGAGCCCGTCACCTTCGGGCTCAAGATGGCCGAA
GCCTATGCCGAGTTTTCGCGTTGCAAGGCGCGGCTCCAGACGGCGCGCGCCGAAATCGCGACCTGTGCCATTTCCGGCGC
GGTCGGCACCTTCGCCAATATCGATCCGCGCGTCGAAGCGCATGTCGCCGCCAAGCTGGGCCTGTCGATCGAGCCCGTCT
CGACGCAGGTGATCCCGCGCGACCGTCACGCGATGTTCTTCGCGGTGCTCGGCGTGATCGCCTCGTCGATCGAGCGGCTT
GCGGTCGAGGTGCGCCACCTTCAGCGCACCGAAGTGCTGGAGGCAGAAGAGTATTTTTCGCCGGGCCAGAAAGGTTCGTC
GGCGATGCCGCACAAGCGCAATCCCGTGCTCACCGAGAACCTCACCGGCCTTGCCCGCATGGTGCGCAGCGCGGTCACGC
CCGCGATGGAGAATGTCGCGCTGTGGCACGAACGCGACATCAGCCATTCGTCGGTCGAACGCTTCATCGGCCCCGACGCG
ACGATCACGCTCGACTTCGCGCTCGCGCGCCTCACCGGGGTCATCGACAAGCTGCTCGTCTATCCCGACCGGATGCAGAA
AAATCTCGATCGCATGGGCGGGCTCGTTCATTCGCAGCGGGTGCTGCTGGCGCTGACGCAGGCGGGCGCCAGCCGCGAAG
ACAGCTATGCGCTCGTCCAGCGCAACGCGATGAAAGTATGGGAAAGCGACGGCCAGCTCTCGCTGCTCGAACTGCTCAAG
TCCGACGCCGACGTCACCGCGCGCCTGTCGGCCGATCAACTCACGGAACTTTTCGACCTCGACTATCATATGAAGCATGT
GGACACGATCTTCGACCGGGTGTTCGGCGCCGCCTAG

Upstream 100 bases:

>100_bases
GCCGCGGCATTTGGCGAAACGCCCCGTCCCTGCTAGGGGGCGCCCCGTCGCGGTTCCGCCGGGAATCCGCGACAAGGCGC
CAGAAAAGGAAGGAAATTCA

Downstream 100 bases:

>100_bases
CGACAGGGTGGAATCGCCGCGCAATCTGTCATACTGTCCGCGCCGGACCGACGAGGGAGAAGACGACGTGGGCATCCTGC
GAACGATCATCTGGGTGGTG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVPRYARPEMTAIWSAENRFSIWFEIEAHATDALAELGTVPPSAAKALWDWWATRPAIDVAAIDAIEAVTKHDVIAFLTW
VAENVGEEARFMHQGMTSSDVLDTCLAVQLAQAADILLADLEALLAAIKRRAFEHKLTPTIGRSHGIHAEPVTFGLKMAE
AYAEFSRCKARLQTARAEIATCAISGAVGTFANIDPRVEAHVAAKLGLSIEPVSTQVIPRDRHAMFFAVLGVIASSIERL
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSAVTPAMENVALWHERDISHSSVERFIGPDA
TITLDFALARLTGVIDKLLVYPDRMQKNLDRMGGLVHSQRVLLALTQAGASREDSYALVQRNAMKVWESDGQLSLLELLK
SDADVTARLSADQLTELFDLDYHMKHVDTIFDRVFGAA

Sequences:

>Translated_438_residues
MVPRYARPEMTAIWSAENRFSIWFEIEAHATDALAELGTVPPSAAKALWDWWATRPAIDVAAIDAIEAVTKHDVIAFLTW
VAENVGEEARFMHQGMTSSDVLDTCLAVQLAQAADILLADLEALLAAIKRRAFEHKLTPTIGRSHGIHAEPVTFGLKMAE
AYAEFSRCKARLQTARAEIATCAISGAVGTFANIDPRVEAHVAAKLGLSIEPVSTQVIPRDRHAMFFAVLGVIASSIERL
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSAVTPAMENVALWHERDISHSSVERFIGPDA
TITLDFALARLTGVIDKLLVYPDRMQKNLDRMGGLVHSQRVLLALTQAGASREDSYALVQRNAMKVWESDGQLSLLELLK
SDADVTARLSADQLTELFDLDYHMKHVDTIFDRVFGAA
>Mature_438_residues
MVPRYARPEMTAIWSAENRFSIWFEIEAHATDALAELGTVPPSAAKALWDWWATRPAIDVAAIDAIEAVTKHDVIAFLTW
VAENVGEEARFMHQGMTSSDVLDTCLAVQLAQAADILLADLEALLAAIKRRAFEHKLTPTIGRSHGIHAEPVTFGLKMAE
AYAEFSRCKARLQTARAEIATCAISGAVGTFANIDPRVEAHVAAKLGLSIEPVSTQVIPRDRHAMFFAVLGVIASSIERL
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSAVTPAMENVALWHERDISHSSVERFIGPDA
TITLDFALARLTGVIDKLLVYPDRMQKNLDRMGGLVHSQRVLLALTQAGASREDSYALVQRNAMKVWESDGQLSLLELLK
SDADVTARLSADQLTELFDLDYHMKHVDTIFDRVFGAA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=419, Percent_Identity=26.491646778043, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=26.2337662337662, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1787376, Length=350, Percent_Identity=29.7142857142857, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=25, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32564234, Length=361, Percent_Identity=26.0387811634349, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323391, Length=416, Percent_Identity=28.8461538461538, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24647570, Length=387, Percent_Identity=27.6485788113695, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48241; Mature: 48241

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPRYARPEMTAIWSAENRFSIWFEIEAHATDALAELGTVPPSAAKALWDWWATRPAIDV
CCCCCCCCCCEEEECCCCCEEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHH
AAIDAIEAVTKHDVIAFLTWVAENVGEEARFMHQGMTSSDVLDTCLAVQLAQAADILLAD
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LEALLAAIKRRAFEHKLTPTIGRSHGIHAEPVTFGLKMAEAYAEFSRCKARLQTARAEIA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCAISGAVGTFANIDPRVEAHVAAKLGLSIEPVSTQVIPRDRHAMFFAVLGVIASSIERL
HHHHHCCHHHCCCCCCCHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSAVTPAMENVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LWHERDISHSSVERFIGPDATITLDFALARLTGVIDKLLVYPDRMQKNLDRMGGLVHSQR
HHHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
VLLALTQAGASREDSYALVQRNAMKVWESDGQLSLLELLKSDADVTARLSADQLTELFDL
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHC
DYHMKHVDTIFDRVFGAA
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVPRYARPEMTAIWSAENRFSIWFEIEAHATDALAELGTVPPSAAKALWDWWATRPAIDV
CCCCCCCCCCEEEECCCCCEEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHH
AAIDAIEAVTKHDVIAFLTWVAENVGEEARFMHQGMTSSDVLDTCLAVQLAQAADILLAD
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LEALLAAIKRRAFEHKLTPTIGRSHGIHAEPVTFGLKMAEAYAEFSRCKARLQTARAEIA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCAISGAVGTFANIDPRVEAHVAAKLGLSIEPVSTQVIPRDRHAMFFAVLGVIASSIERL
HHHHHCCHHHCCCCCCCHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSAVTPAMENVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LWHERDISHSSVERFIGPDATITLDFALARLTGVIDKLLVYPDRMQKNLDRMGGLVHSQR
HHHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
VLLALTQAGASREDSYALVQRNAMKVWESDGQLSLLELLKSDADVTARLSADQLTELFDL
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHC
DYHMKHVDTIFDRVFGAA
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]