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The map label for this gene is fadA [H]

Identifier: 103486494

GI number: 103486494

Start: 1029325

End: 1030485

Strand: Reverse

Name: fadA [H]

Synonym: Sala_1005

Alternate gene names: 103486494

Gene position: 1030485-1029325 (Counterclockwise)

Preceding gene: 103486495

Following gene: 103486493

Centisome position: 30.81

GC content: 71.58

Gene sequence:

>1161_bases
TTGGCGGACGTCTTTCTCTATGACGCCGTCCGCACGCCACGCGGCAAGGCACGGCCCGACGGCGGGCTCGCGAACCTCAG
CCCGCAGGAACTGGTGCGCCAGCAGGCCGCGGCACTGGCGGCGCGCTGCGGCGATGTGGCCGCGCACCCCGACGCGCTGA
TCCTCGGCTGCGTGACGCAGACCGGCGCGCAGGGTGGGCATATCGCGCTCGTCGCCAAGCTCCACGCCGACCTGCCCGAC
ACCATGGCGGCGCACAGCCTCAACAATTATTGCGCCTCCGGACTCACGGCGATCGGACTGGCGGTCGCAAAGGTCGCGAG
CGGCGAGATCGACGTGGCGCTCGCCGGCGGCGTCGAATCGATGAGCGCGGCGCCCTTTCTGAGCGATCATGCGGGCTTCT
ACGCCAATGACGAGCTGCCGCCGCGCGCGCGCTTCGTGCCGCCGGTGCTCGGCGCCGACCGACTCGCGCACGCCGAAGGC
ATCACGCGCGCCGAACTCGACGCGGTTGCCCTCGCCTCGCAGCGCAAGGCGGCAATCGCCGAAGGCGATGCCGCGCTCCA
GAAATCGCGGATAGCGACGGGCGCGCTCGCGGGCGAGGAATGTATCCGGCCGCAGACGAGCGCCGAATCGCTCGCGGCGA
TGCCCGCTGCCTTCGGCGCATTGCAGGCGGAATATCCAGACGCACTCGAAGGCACGCGCTTCGCGCCGCTGCACAGCGTC
GCGCACGCCCCGCCCATCTGCGACGGCGCGGGGCTGGCGCTCGTCGCGCGTGCGGCGCCTGGCCCCCCTGCCCGCGCGCG
CGTCGTCGCCTTTGCCGAAAGCGGCGGCGATCCGGTCGCGTCGCTGACCGCGGGATTTGCGGCGATGGACAAGGTGCTCG
TGCGCGCCGGACTGTCGCTCGCCGACATCGACCGGATCGAGTTCATGGAGGCGTTCGCGGTCACCATCGCGAAGTTCCTG
CGCGACCGCGACGTCGACCCGGAGCGGGTCAATGTCAGCGGTGGGCATCTGGCCAAGGGTCATCCGATGGGCGCGAGCGG
CGCGATCCTGACCTCGACCTTGCTCGACGCGCTCGATGCGTGTCGGGGCCGATACGGGCTGGTCGTGCTGACCGGCGCGA
TGGGGGTCGGCGCGGCGATGGTGGTCGAACGGGCGGCGTAA

Upstream 100 bases:

>100_bases
AACGCTGGCGATTATCAGGTGGAAGGGGCGCGCAATTTCGCCACCTTCAACGTCGGCGGCAGCGCGACAACCGCGGTCAG
CTTCGTCGTCGGCACCTGAT

Downstream 100 bases:

>100_bases
ACATTCGCCGCGTCGACGTGCGATGGGGAGGATCAATGTGTCGAAAACCCCATTGCACCCCCGCCGCATTCCGCTATGTG
CGGCGCGTGCGGGGCTTGCC

Product: Acetyl-CoA C-acyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MADVFLYDAVRTPRGKARPDGGLANLSPQELVRQQAAALAARCGDVAAHPDALILGCVTQTGAQGGHIALVAKLHADLPD
TMAAHSLNNYCASGLTAIGLAVAKVASGEIDVALAGGVESMSAAPFLSDHAGFYANDELPPRARFVPPVLGADRLAHAEG
ITRAELDAVALASQRKAAIAEGDAALQKSRIATGALAGEECIRPQTSAESLAAMPAAFGALQAEYPDALEGTRFAPLHSV
AHAPPICDGAGLALVARAAPGPPARARVVAFAESGGDPVASLTAGFAAMDKVLVRAGLSLADIDRIEFMEAFAVTIAKFL
RDRDVDPERVNVSGGHLAKGHPMGASGAILTSTLLDALDACRGRYGLVVLTGAMGVGAAMVVERAA

Sequences:

>Translated_386_residues
MADVFLYDAVRTPRGKARPDGGLANLSPQELVRQQAAALAARCGDVAAHPDALILGCVTQTGAQGGHIALVAKLHADLPD
TMAAHSLNNYCASGLTAIGLAVAKVASGEIDVALAGGVESMSAAPFLSDHAGFYANDELPPRARFVPPVLGADRLAHAEG
ITRAELDAVALASQRKAAIAEGDAALQKSRIATGALAGEECIRPQTSAESLAAMPAAFGALQAEYPDALEGTRFAPLHSV
AHAPPICDGAGLALVARAAPGPPARARVVAFAESGGDPVASLTAGFAAMDKVLVRAGLSLADIDRIEFMEAFAVTIAKFL
RDRDVDPERVNVSGGHLAKGHPMGASGAILTSTLLDALDACRGRYGLVVLTGAMGVGAAMVVERAA
>Mature_385_residues
ADVFLYDAVRTPRGKARPDGGLANLSPQELVRQQAAALAARCGDVAAHPDALILGCVTQTGAQGGHIALVAKLHADLPDT
MAAHSLNNYCASGLTAIGLAVAKVASGEIDVALAGGVESMSAAPFLSDHAGFYANDELPPRARFVPPVLGADRLAHAEGI
TRAELDAVALASQRKAAIAEGDAALQKSRIATGALAGEECIRPQTSAESLAAMPAAFGALQAEYPDALEGTRFAPLHSVA
HAPPICDGAGLALVARAAPGPPARARVVAFAESGGDPVASLTAGFAAMDKVLVRAGLSLADIDRIEFMEAFAVTIAKFLR
DRDVDPERVNVSGGHLAKGHPMGASGAILTSTLLDALDACRGRYGLVVLTGAMGVGAAMVVERAA

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=410, Percent_Identity=31.4634146341463, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI167614485, Length=408, Percent_Identity=29.1666666666667, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI4504327, Length=442, Percent_Identity=30.5429864253394, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI148539872, Length=337, Percent_Identity=28.1899109792285, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=27.7915632754342, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1787663, Length=416, Percent_Identity=32.4519230769231, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI48994986, Length=407, Percent_Identity=33.4152334152334, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87082165, Length=411, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788554, Length=410, Percent_Identity=34.1463414634146, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788683, Length=366, Percent_Identity=27.5956284153005, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535921, Length=399, Percent_Identity=29.3233082706767, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI133906874, Length=411, Percent_Identity=30.4136253041363, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI25147385, Length=394, Percent_Identity=27.9187817258883, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17551802, Length=439, Percent_Identity=26.1958997722096, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322031, Length=321, Percent_Identity=29.2834890965732, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325229, Length=422, Percent_Identity=27.7251184834123, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24655093, Length=409, Percent_Identity=30.0733496332518, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI17648125, Length=405, Percent_Identity=26.9135802469136, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640423, Length=403, Percent_Identity=29.0322580645161, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 39209; Mature: 39078

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVFLYDAVRTPRGKARPDGGLANLSPQELVRQQAAALAARCGDVAAHPDALILGCVTQ
CCCEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHH
TGAQGGHIALVAKLHADLPDTMAAHSLNNYCASGLTAIGLAVAKVASGEIDVALAGGVES
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
MSAAPFLSDHAGFYANDELPPRARFVPPVLGADRLAHAEGITRAELDAVALASQRKAAIA
HCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EGDAALQKSRIATGALAGEECIRPQTSAESLAAMPAAFGALQAEYPDALEGTRFAPLHSV
CCHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHH
AHAPPICDGAGLALVARAAPGPPARARVVAFAESGGDPVASLTAGFAAMDKVLVRAGLSL
HCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCH
ADIDRIEFMEAFAVTIAKFLRDRDVDPERVNVSGGHLAKGHPMGASGAILTSTLLDALDA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
CRGRYGLVVLTGAMGVGAAMVVERAA
HCCCCCEEEEECCHHCCHHHHHCCCC
>Mature Secondary Structure 
ADVFLYDAVRTPRGKARPDGGLANLSPQELVRQQAAALAARCGDVAAHPDALILGCVTQ
CCEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHH
TGAQGGHIALVAKLHADLPDTMAAHSLNNYCASGLTAIGLAVAKVASGEIDVALAGGVES
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
MSAAPFLSDHAGFYANDELPPRARFVPPVLGADRLAHAEGITRAELDAVALASQRKAAIA
HCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EGDAALQKSRIATGALAGEECIRPQTSAESLAAMPAAFGALQAEYPDALEGTRFAPLHSV
CCHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHH
AHAPPICDGAGLALVARAAPGPPARARVVAFAESGGDPVASLTAGFAAMDKVLVRAGLSL
HCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCH
ADIDRIEFMEAFAVTIAKFLRDRDVDPERVNVSGGHLAKGHPMGASGAILTSTLLDALDA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
CRGRYGLVVLTGAMGVGAAMVVERAA
HCCCCCEEEEECCHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]