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The map label for this gene is btuB [H]

Identifier: 103486493

GI number: 103486493

Start: 1026974

End: 1028830

Strand: Reverse

Name: btuB [H]

Synonym: Sala_1004

Alternate gene names: 103486493

Gene position: 1028830-1026974 (Counterclockwise)

Preceding gene: 103486494

Following gene: 103486492

Centisome position: 30.76

GC content: 64.84

Gene sequence:

>1857_bases
ATGTTCAGATATTCGACCATTTCCATGATCGCCCTTGCCGTTGCGACCCCTGTCGTTGCGCAGGAAACCAACCCCGATGC
CGACGACATCGTCGTTACCGCATCGGGCATCGAACAGCCCGGCGACGAGGTCGGTCAGGCGATCACGGTGATCGACGCCG
ACACGATCGAAACGCGGCAAACGATCGACGTCGTCGACCTGCTCGCAACGACGCCCGGCATCCGATTCAATCGCACCGGC
ACCACCGGATCGGTCACCGGCGTGTCGATCCGCGGCGCCGAGACGACGCAGACCCTGGTGCTGATCGACGGGGTCAAGGT
CAACGACCCCAGCGGCATCGGCGACGGTTATGATTTCGGCCATCTGCTCACCGGCAATATCCGCCGCATCGAGGTGCTGC
GCGGATCAAACTCGGTCGTCCATGGCAGCCAGGCGATCGGCGGCGTGGTCAATGTGATGACGGCGACGCCCGGCTCGGGT
TTCGCAGGCGGCGCATCGGCCGAATATGGCTATAGCGACACCGTCAACGCCAGGGCCGACCTGTCGGGCACCACCGGGCC
GGTGTCGGGCAGCATCGGTGGCGCCTATTTCCGCACCGACGGCATTTCGTCGGCGGCGGTCGGCACCGAGCGCGACGGAT
ATGAAAATATCGCCGCCAATGCGCGGCTGAAGGTCGCGTTCAGCGACGCGCTGAGCCTTGATCTGCGCGGCTATTACATC
AACGCCGACCTCGATTTCGACAGCTTCTTCGGCGCTCCGGCCGACAGCGCCGACGTCGCCAGGCTCGACCAATATGTCGG
CTATGCCGGGCTGAACCTCGGCCTCTTCGACGGTCGCTTCACCAGTCGCGCCGCGGTGACATGGATGCGCAACGAGCGCG
ACTATTTCTTCGTGCGCGGCACCGCCCCCGACTTTGGCTATAAGGGCACGAACCTGCGCTTCGAATATCAGGGCGTCGTG
GCCCCCGCCGACACCGCCAGGCTGGTGTTCGGTTACGAGCATGAGCGCCCCGAATATGACTTCTTCGGCTTTGGTTCGAC
CGACAGCCAGAAGGCCAATATCGACAGCGTCTATGCACTCGCCATCGTCCAGCCGCTCGCCGGCCTGTCGCTGACCGGCG
GCGTTCGCCACGACGACCATAACCAGTTCGGCGGCGCCACGACCTTTGGCGCCAACGCCAATTATTCGCCCAACGGCGGC
GCCACCAATGTCCGCTTGAGCTATGGCGAGGGGTTCAAGGCGCCGTCGCTCTTTCAGCTCTACGACAGCTTCAGCGGCAA
TGCGGCGCTGCGTCCCGAACGCTCGAAAAGCTATGACGTCGGCATCGACCAGAGCCTCGCCGACGGGCGCGCGCTGGTGT
CGCTCACCGCCTTCCTGCGCAACACGACCGACCAGATCAATTTCGACAATGCGACCTTCACCTATGGCAATATCGACCGC
ACGCGCGCCAAGGGGATCGAGGCGACGCTGGCGCTGAAACCCGTCGACGCACTGAACGTCACGGCGTCGTACAGCTATAT
CGACGCGCGCGACCGGTCGGGGCGGCCCGCGTTCGACGGCAAGCGCCTGCCGCGCCGCGCCGCGCATGCGGTCAGCCTGT
CGGCGGACCATGACTGGTCGTTCGGGCTGTCGGCCGGCGCGACGGTCACGATGGTCGGCGACAGTTTCGACAATGCGACG
AATATGGTGCGGCTCGACGGCTATGCGCTCGCCGGGGTGCGCGCGTCGTTCGCGGTTACCGAGCGGATCGAAGTGTACGG
CCGCGTCGATAATCTGTTCGACGCCGATTATGCGACGGCGTTCAACTTTGGCACCTATGGCCGCGCCGCCCATGGCGGGG
TGCGGGCGCGATTCTGA

Upstream 100 bases:

>100_bases
GGCGTCGCGGGTCTTTACGCCGCTTGCGACAAAGGCATCGGTCGACCGCTCACGCGGTTCGGCAGGCTCCGCCTTCGTTG
CCGGCGTGCGGGAGTCTCCC

Downstream 100 bases:

>100_bases
TGGCGCGGCGGGGGAGATGGGTATGAGCGGGATAGCGAGGTGATGGCGATCAGGCCCGCGCTGATCGGCGCGGCGCTGAT
CGGCGCCGCGGGGGTGCTGG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MFRYSTISMIALAVATPVVAQETNPDADDIVVTASGIEQPGDEVGQAITVIDADTIETRQTIDVVDLLATTPGIRFNRTG
TTGSVTGVSIRGAETTQTLVLIDGVKVNDPSGIGDGYDFGHLLTGNIRRIEVLRGSNSVVHGSQAIGGVVNVMTATPGSG
FAGGASAEYGYSDTVNARADLSGTTGPVSGSIGGAYFRTDGISSAAVGTERDGYENIAANARLKVAFSDALSLDLRGYYI
NADLDFDSFFGAPADSADVARLDQYVGYAGLNLGLFDGRFTSRAAVTWMRNERDYFFVRGTAPDFGYKGTNLRFEYQGVV
APADTARLVFGYEHERPEYDFFGFGSTDSQKANIDSVYALAIVQPLAGLSLTGGVRHDDHNQFGGATTFGANANYSPNGG
ATNVRLSYGEGFKAPSLFQLYDSFSGNAALRPERSKSYDVGIDQSLADGRALVSLTAFLRNTTDQINFDNATFTYGNIDR
TRAKGIEATLALKPVDALNVTASYSYIDARDRSGRPAFDGKRLPRRAAHAVSLSADHDWSFGLSAGATVTMVGDSFDNAT
NMVRLDGYALAGVRASFAVTERIEVYGRVDNLFDADYATAFNFGTYGRAAHGGVRARF

Sequences:

>Translated_618_residues
MFRYSTISMIALAVATPVVAQETNPDADDIVVTASGIEQPGDEVGQAITVIDADTIETRQTIDVVDLLATTPGIRFNRTG
TTGSVTGVSIRGAETTQTLVLIDGVKVNDPSGIGDGYDFGHLLTGNIRRIEVLRGSNSVVHGSQAIGGVVNVMTATPGSG
FAGGASAEYGYSDTVNARADLSGTTGPVSGSIGGAYFRTDGISSAAVGTERDGYENIAANARLKVAFSDALSLDLRGYYI
NADLDFDSFFGAPADSADVARLDQYVGYAGLNLGLFDGRFTSRAAVTWMRNERDYFFVRGTAPDFGYKGTNLRFEYQGVV
APADTARLVFGYEHERPEYDFFGFGSTDSQKANIDSVYALAIVQPLAGLSLTGGVRHDDHNQFGGATTFGANANYSPNGG
ATNVRLSYGEGFKAPSLFQLYDSFSGNAALRPERSKSYDVGIDQSLADGRALVSLTAFLRNTTDQINFDNATFTYGNIDR
TRAKGIEATLALKPVDALNVTASYSYIDARDRSGRPAFDGKRLPRRAAHAVSLSADHDWSFGLSAGATVTMVGDSFDNAT
NMVRLDGYALAGVRASFAVTERIEVYGRVDNLFDADYATAFNFGTYGRAAHGGVRARF
>Mature_618_residues
MFRYSTISMIALAVATPVVAQETNPDADDIVVTASGIEQPGDEVGQAITVIDADTIETRQTIDVVDLLATTPGIRFNRTG
TTGSVTGVSIRGAETTQTLVLIDGVKVNDPSGIGDGYDFGHLLTGNIRRIEVLRGSNSVVHGSQAIGGVVNVMTATPGSG
FAGGASAEYGYSDTVNARADLSGTTGPVSGSIGGAYFRTDGISSAAVGTERDGYENIAANARLKVAFSDALSLDLRGYYI
NADLDFDSFFGAPADSADVARLDQYVGYAGLNLGLFDGRFTSRAAVTWMRNERDYFFVRGTAPDFGYKGTNLRFEYQGVV
APADTARLVFGYEHERPEYDFFGFGSTDSQKANIDSVYALAIVQPLAGLSLTGGVRHDDHNQFGGATTFGANANYSPNGG
ATNVRLSYGEGFKAPSLFQLYDSFSGNAALRPERSKSYDVGIDQSLADGRALVSLTAFLRNTTDQINFDNATFTYGNIDR
TRAKGIEATLALKPVDALNVTASYSYIDARDRSGRPAFDGKRLPRRAAHAVSLSADHDWSFGLSAGATVTMVGDSFDNAT
NMVRLDGYALAGVRASFAVTERIEVYGRVDNLFDADYATAFNFGTYGRAAHGGVRARF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=629, Percent_Identity=27.9809220985692, Blast_Score=199, Evalue=6e-52,
Organism=Escherichia coli, GI1787723, Length=683, Percent_Identity=25.0366032210835, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788478, Length=175, Percent_Identity=32.5714285714286, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1787024, Length=297, Percent_Identity=23.9057239057239, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 65682; Mature: 65682

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRYSTISMIALAVATPVVAQETNPDADDIVVTASGIEQPGDEVGQAITVIDADTIETRQ
CCEEHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCHHHCCEEEEEECCCCCCCH
TIDVVDLLATTPGIRFNRTGTTGSVTGVSIRGAETTQTLVLIDGVKVNDPSGIGDGYDFG
HHHHHHHHHCCCCCEEECCCCCCCEEEEEEECCCCCEEEEEEECEEECCCCCCCCCCCCC
HLLTGNIRRIEVLRGSNSVVHGSQAIGGVVNVMTATPGSGFAGGASAEYGYSDTVNARAD
EEEECCCEEEEEEECCCCEEECCHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEC
LSGTTGPVSGSIGGAYFRTDGISSAAVGTERDGYENIAANARLKVAFSDALSLDLRGYYI
CCCCCCCCCCCCCCEEEEECCCCHHCCCCCCCCCCHHCCCCEEEEEEECCEEEEEEEEEE
NADLDFDSFFGAPADSADVARLDQYVGYAGLNLGLFDGRFTSRAAVTWMRNERDYFFVRG
EEECCHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEE
TAPDFGYKGTNLRFEYQGVVAPADTARLVFGYEHERPEYDFFGFGSTDSQKANIDSVYAL
CCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCHHHHHH
AIVQPLAGLSLTGGVRHDDHNQFGGATTFGANANYSPNGGATNVRLSYGEGFKAPSLFQL
HHHHHHCCCEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
YDSFSGNAALRPERSKSYDVGIDQSLADGRALVSLTAFLRNTTDQINFDNATFTYGNIDR
HHCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCEEEECCCCH
TRAKGIEATLALKPVDALNVTASYSYIDARDRSGRPAFDGKRLPRRAAHAVSLSADHDWS
HHHCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHEEEECCCCCCC
FGLSAGATVTMVGDSFDNATNMVRLDGYALAGVRASFAVTERIEVYGRVDNLFDADYATA
CCCCCCCEEEEECCCCCCCCCEEEECCEEEECCEEEEEEEEEEEEEECCCCCCCCCHHEE
FNFGTYGRAAHGGVRARF
ECCCCCCCCCCCCEEECC
>Mature Secondary Structure
MFRYSTISMIALAVATPVVAQETNPDADDIVVTASGIEQPGDEVGQAITVIDADTIETRQ
CCEEHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCHHHCCEEEEEECCCCCCCH
TIDVVDLLATTPGIRFNRTGTTGSVTGVSIRGAETTQTLVLIDGVKVNDPSGIGDGYDFG
HHHHHHHHHCCCCCEEECCCCCCCEEEEEEECCCCCEEEEEEECEEECCCCCCCCCCCCC
HLLTGNIRRIEVLRGSNSVVHGSQAIGGVVNVMTATPGSGFAGGASAEYGYSDTVNARAD
EEEECCCEEEEEEECCCCEEECCHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEC
LSGTTGPVSGSIGGAYFRTDGISSAAVGTERDGYENIAANARLKVAFSDALSLDLRGYYI
CCCCCCCCCCCCCCEEEEECCCCHHCCCCCCCCCCHHCCCCEEEEEEECCEEEEEEEEEE
NADLDFDSFFGAPADSADVARLDQYVGYAGLNLGLFDGRFTSRAAVTWMRNERDYFFVRG
EEECCHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEE
TAPDFGYKGTNLRFEYQGVVAPADTARLVFGYEHERPEYDFFGFGSTDSQKANIDSVYAL
CCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCHHHHHH
AIVQPLAGLSLTGGVRHDDHNQFGGATTFGANANYSPNGGATNVRLSYGEGFKAPSLFQL
HHHHHHCCCEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
YDSFSGNAALRPERSKSYDVGIDQSLADGRALVSLTAFLRNTTDQINFDNATFTYGNIDR
HHCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCEEEECCCCH
TRAKGIEATLALKPVDALNVTASYSYIDARDRSGRPAFDGKRLPRRAAHAVSLSADHDWS
HHHCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHEEEECCCCCCC
FGLSAGATVTMVGDSFDNATNMVRLDGYALAGVRASFAVTERIEVYGRVDNLFDADYATA
CCCCCCCEEEEECCCCCCCCCEEEECCEEEECCEEEEEEEEEEEEEECCCCCCCCCHHEE
FNFGTYGRAAHGGVRARF
ECCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA