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The map label for this gene is smeIM [H]

Identifier: 103486455

GI number: 103486455

Start: 991065

End: 992204

Strand: Direct

Name: smeIM [H]

Synonym: Sala_0965

Alternate gene names: 103486455

Gene position: 991065-992204 (Clockwise)

Preceding gene: 103486454

Following gene: 103486456

Centisome position: 29.63

GC content: 65.09

Gene sequence:

>1140_bases
ATGGGTGTGATGGAACGGGTGAAGGCGCCGGCGAGGAAGCTGCGGACGCCGGCGGTTGCGCCCGCGGACCTGCCGCTCGA
CAGCATCCTGCAGGGCGATTGCGTCGCGATGATGCGCGGCCTGCCCGCGGCGTCGGTCGACATGATCTTTGCCGATCCGC
CGTACAACCTTCAGCTCGGTGGCGATTTGCTGCGCCCCGACGGCAGTCAGGTCGATGCGGTCGACGACGACTGGGACAAG
TTCGACAGTCTGGCGACCTATGACCGCTTCACCCATGCCTGGCTGAAGGAAGCCAGGCGTATCCTGAAACCCGGCGGCAG
CATCTGGGTCATCGGCAGCTATCACAATATCTTCCGCGTCGGCGCCGCGCTCCAGGACCAGGGATACTGGATCCTCAACG
ACATCGTGTGGCGCAAGGCGAATCCGATGCCCAATTTCAAGGGCACGCGCTTTACCAACGCGCATGAGACGCTGATCTGG
GCGTCGATGGGCGAGAAGGCGCGCTATACCTTCAACTATCGCGCGATGAAGACGCTGAACGACGAGCTTCAGATGCGCAG
CGACTGGCTGATCCCGATCTGCGGCGGGCCGGAACGGCTGAAGAAGGGCGGGCACAAGGTCCACCCGACGCAAAAGCCCG
AGGCCTTGCTCTATCGCATCCTGCTCGCCTGCTCGAACCCCGGCGACGTGATCCTCGATCCCTTTTTCGGAACCGGCACG
ACGGGCGCGGTCGCGAAGCGGCTGGGGCGCCACTATATCGGCATCGAGCGCGAGGATGACTATATCGCGGCGGCGAAGGA
GCGTATCGAACTGGCGCTGCCGCTCGACGAAAGCGCGGTGAAGACGATGATGGCGCCCAAGGCCGCCACGCGCGTCGCGT
TTGGCACGCTCGTCGAATGCGGGTTGATCGCGCCGGGGACCGTGCTGACCGATGCCAAGCGCCGCTGGAAAGCGAAAGTG
CGCGTCGATGGCAGCCTCGATTGCGAGGGGCAGGCGCCGGGGTCGATCCACAAGGTCGGCGCGGGTGTGCAGGGTGCGCC
GAGCTGCAACGGCTGGACCTTCTGGCATGTCGATACGGGTCAGGAGTTGCGCGTGATCGACGCGGTGCGGCAGGACTGGT
TGCTGGCGAACGAGGCCTGA

Upstream 100 bases:

>100_bases
TTAACCATTGTGACACCGGGAATCCCTAGAATCCGTGGCTTGACGGCGGACTCTGCCGGACTCATCAGGGACATCTTTCG
ATCGAGCAACGGGGTGACGC

Downstream 100 bases:

>100_bases
CATCATACGTCATTGCGAGGAGCGGAGCGACGAAGCAATCTCCACCTATCGGCGCGCGTCCCCGGTGGCTGGAGATTGCT
TCGCTTCGCTCGCAATGACG

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MGVMERVKAPARKLRTPAVAPADLPLDSILQGDCVAMMRGLPAASVDMIFADPPYNLQLGGDLLRPDGSQVDAVDDDWDK
FDSLATYDRFTHAWLKEARRILKPGGSIWVIGSYHNIFRVGAALQDQGYWILNDIVWRKANPMPNFKGTRFTNAHETLIW
ASMGEKARYTFNYRAMKTLNDELQMRSDWLIPICGGPERLKKGGHKVHPTQKPEALLYRILLACSNPGDVILDPFFGTGT
TGAVAKRLGRHYIGIEREDDYIAAAKERIELALPLDESAVKTMMAPKAATRVAFGTLVECGLIAPGTVLTDAKRRWKAKV
RVDGSLDCEGQAPGSIHKVGAGVQGAPSCNGWTFWHVDTGQELRVIDAVRQDWLLANEA

Sequences:

>Translated_379_residues
MGVMERVKAPARKLRTPAVAPADLPLDSILQGDCVAMMRGLPAASVDMIFADPPYNLQLGGDLLRPDGSQVDAVDDDWDK
FDSLATYDRFTHAWLKEARRILKPGGSIWVIGSYHNIFRVGAALQDQGYWILNDIVWRKANPMPNFKGTRFTNAHETLIW
ASMGEKARYTFNYRAMKTLNDELQMRSDWLIPICGGPERLKKGGHKVHPTQKPEALLYRILLACSNPGDVILDPFFGTGT
TGAVAKRLGRHYIGIEREDDYIAAAKERIELALPLDESAVKTMMAPKAATRVAFGTLVECGLIAPGTVLTDAKRRWKAKV
RVDGSLDCEGQAPGSIHKVGAGVQGAPSCNGWTFWHVDTGQELRVIDAVRQDWLLANEA
>Mature_378_residues
GVMERVKAPARKLRTPAVAPADLPLDSILQGDCVAMMRGLPAASVDMIFADPPYNLQLGGDLLRPDGSQVDAVDDDWDKF
DSLATYDRFTHAWLKEARRILKPGGSIWVIGSYHNIFRVGAALQDQGYWILNDIVWRKANPMPNFKGTRFTNAHETLIWA
SMGEKARYTFNYRAMKTLNDELQMRSDWLIPICGGPERLKKGGHKVHPTQKPEALLYRILLACSNPGDVILDPFFGTGTT
GAVAKRLGRHYIGIEREDDYIAAAKERIELALPLDESAVKTMMAPKAATRVAFGTLVECGLIAPGTVLTDAKRRWKAKVR
VDGSLDCEGQAPGSIHKVGAGVQGAPSCNGWTFWHVDTGQELRVIDAVRQDWLLANEA

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=267, Percent_Identity=32.9588014981273, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 41852; Mature: 41720

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVMERVKAPARKLRTPAVAPADLPLDSILQGDCVAMMRGLPAASVDMIFADPPYNLQLG
CCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCEEEEECCCCCCEEEC
GDLLRPDGSQVDAVDDDWDKFDSLATYDRFTHAWLKEARRILKPGGSIWVIGSYHNIFRV
CCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
GAALQDQGYWILNDIVWRKANPMPNFKGTRFTNAHETLIWASMGEKARYTFNYRAMKTLN
CCHHCCCCCEEEEHHHHHCCCCCCCCCCCEECCCCCEEEEECCCCCEEEEEEEHHHHHHH
DELQMRSDWLIPICGGPERLKKGGHKVHPTQKPEALLYRILLACSNPGDVILDPFFGTGT
HHHHHCCCCEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCC
TGAVAKRLGRHYIGIEREDDYIAAAKERIELALPLDESAVKTMMAPKAATRVAFGTLVEC
HHHHHHHHCHHHCCCCCCCCHHHHHHHCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHH
GLIAPGTVLTDAKRRWKAKVRVDGSLDCEGQAPGSIHKVGAGVQGAPSCNGWTFWHVDTG
CCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCC
QELRVIDAVRQDWLLANEA
CCCHHHHHHHHHCEECCCC
>Mature Secondary Structure 
GVMERVKAPARKLRTPAVAPADLPLDSILQGDCVAMMRGLPAASVDMIFADPPYNLQLG
CHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCEEEEECCCCCCEEEC
GDLLRPDGSQVDAVDDDWDKFDSLATYDRFTHAWLKEARRILKPGGSIWVIGSYHNIFRV
CCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
GAALQDQGYWILNDIVWRKANPMPNFKGTRFTNAHETLIWASMGEKARYTFNYRAMKTLN
CCHHCCCCCEEEEHHHHHCCCCCCCCCCCEECCCCCEEEEECCCCCEEEEEEEHHHHHHH
DELQMRSDWLIPICGGPERLKKGGHKVHPTQKPEALLYRILLACSNPGDVILDPFFGTGT
HHHHHCCCCEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCC
TGAVAKRLGRHYIGIEREDDYIAAAKERIELALPLDESAVKTMMAPKAATRVAFGTLVEC
HHHHHHHHCHHHCCCCCCCCHHHHHHHCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHH
GLIAPGTVLTDAKRRWKAKVRVDGSLDCEGQAPGSIHKVGAGVQGAPSCNGWTFWHVDTG
CCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCC
QELRVIDAVRQDWLLANEA
CCCHHHHHHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]