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The map label for this gene is rnhB

Identifier: 103486453

GI number: 103486453

Start: 989301

End: 989897

Strand: Direct

Name: rnhB

Synonym: Sala_0963

Alternate gene names: 103486453

Gene position: 989301-989897 (Clockwise)

Preceding gene: 103486452

Following gene: 103486454

Centisome position: 29.57

GC content: 66.5

Gene sequence:

>597_bases
ATGCGAACGGATTCCGGCGTTTGGCTTACTGTGGTGGCGCATATGATCGTCGGCGTCGATGAAGCCGGGCGCGGGCCGCT
TGCGGGGCCGGTCGTCGCGGCGGCGGTGCTGCTGTGCGAGGGGGGCATTGCAGGGCTCGACGATTCGAAGAAGCTCTCCG
CCAGACGTCGCGGCGCGCTGGAAAGCGCGATCAGGGCGCAGTGCCGCTGGGGTATTGGCGAGGCGAGCGTCGAGGAAATC
GACCGGATCAACATATTGCAGGCGACCTTCCTGGCGATGACGCGCGCGGTCGAGGCTTTGGGGTTCGAGCCCGCGGAAGT
GCTGGTCGACGGCAACCGGCTGCCCCGCTGGCGCTACCAGGCGCGCGCAATCGTCGGCGGCGATGCGCTGCACCCCTGTA
TCTCGGCGGCGAGCATCCTTGCCAAACAGCATCGCGACCGCTTGATGATCGCGGCAGCGCAGGATTATCCGGCTTTTGGT
TGGGAGAGCAACATGGGCTATGGTACCGCGCAGCATCTCGCGGCGCTGCGTCGCCATGGCCCGACGCCGCACCATCGGAC
CAGTTTCGCCCCGGTTGCGCAATTGCAACTGGTCTGA

Upstream 100 bases:

>100_bases
GTGTAAACTTCGAAACGATATCATCGGTCCGTTCGTGTCGAGCGAAGTCGAGACACCCATCGGCTTGGTGCAACGCTCAT
GGGCATCGCGACTTCGCTCG

Downstream 100 bases:

>100_bases
CCGCCCACAGCCTGAAAGGGGAAGGCGATGAGCAGGGGATTTTCCGCCGATGACGTAGGGCCGCAAATCGGGCGGCGCTT
TCTGGTCACCGGCGCCAATG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MRTDSGVWLTVVAHMIVGVDEAGRGPLAGPVVAAAVLLCEGGIAGLDDSKKLSARRRGALESAIRAQCRWGIGEASVEEI
DRINILQATFLAMTRAVEALGFEPAEVLVDGNRLPRWRYQARAIVGGDALHPCISAASILAKQHRDRLMIAAAQDYPAFG
WESNMGYGTAQHLAALRRHGPTPHHRTSFAPVAQLQLV

Sequences:

>Translated_198_residues
MRTDSGVWLTVVAHMIVGVDEAGRGPLAGPVVAAAVLLCEGGIAGLDDSKKLSARRRGALESAIRAQCRWGIGEASVEEI
DRINILQATFLAMTRAVEALGFEPAEVLVDGNRLPRWRYQARAIVGGDALHPCISAASILAKQHRDRLMIAAAQDYPAFG
WESNMGYGTAQHLAALRRHGPTPHHRTSFAPVAQLQLV
>Mature_198_residues
MRTDSGVWLTVVAHMIVGVDEAGRGPLAGPVVAAAVLLCEGGIAGLDDSKKLSARRRGALESAIRAQCRWGIGEASVEEI
DRINILQATFLAMTRAVEALGFEPAEVLVDGNRLPRWRYQARAIVGGDALHPCISAASILAKQHRDRLMIAAAQDYPAFG
WESNMGYGTAQHLAALRRHGPTPHHRTSFAPVAQLQLV

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=52.7777777777778, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_SPHAL (Q1GUJ1)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616014.1
- ProteinModelPortal:   Q1GUJ1
- SMR:   Q1GUJ1
- GeneID:   4079963
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0963
- NMPDR:   fig|317655.9.peg.904
- HOGENOM:   HBG584843
- OMA:   IICGVDE
- ProtClustDB:   PRK00015
- BioCyc:   SALA317655:SALA_0963-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21263; Mature: 21263

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTDSGVWLTVVAHMIVGVDEAGRGPLAGPVVAAAVLLCEGGIAGLDDSKKLSARRRGAL
CCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
ESAIRAQCRWGIGEASVEEIDRINILQATFLAMTRAVEALGFEPAEVLVDGNRLPRWRYQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCCHHHH
ARAIVGGDALHPCISAASILAKQHRDRLMIAAAQDYPAFGWESNMGYGTAQHLAALRRHG
HHEEECCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCC
PTPHHRTSFAPVAQLQLV
CCCCCCCCCCCHHHEECC
>Mature Secondary Structure
MRTDSGVWLTVVAHMIVGVDEAGRGPLAGPVVAAAVLLCEGGIAGLDDSKKLSARRRGAL
CCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
ESAIRAQCRWGIGEASVEEIDRINILQATFLAMTRAVEALGFEPAEVLVDGNRLPRWRYQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCCHHHH
ARAIVGGDALHPCISAASILAKQHRDRLMIAAAQDYPAFGWESNMGYGTAQHLAALRRHG
HHEEECCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCC
PTPHHRTSFAPVAQLQLV
CCCCCCCCCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA