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The map label for this gene is theD [H]

Identifier: 103486452

GI number: 103486452

Start: 988058

End: 989206

Strand: Direct

Name: theD [H]

Synonym: Sala_0962

Alternate gene names: 103486452

Gene position: 988058-989206 (Clockwise)

Preceding gene: 103486451

Following gene: 103486453

Centisome position: 29.54

GC content: 69.8

Gene sequence:

>1149_bases
GTGCCGACGACCCCCCGCGTGCTGATCATCGCCGGATCGGACAGCGGCGGCGGAGCGGGAATCCAGGCCGATATCAAGGC
GGTGACGATGCTGGGCGGTCATGCGATGACCGCGATCACCGCGATCACCGCGCAGAACACGCTGGGCGTGCAGGCTGTGC
ATCGGGTGCCCACCGAGATGGTGCTGGCGCAGATCGACAGCGTTGCGGCCGACATCGGTGTTGACGCTGTCAAGATCGGC
ATGATCGGCAGCGCCGAAACCGCGGCGGCGGTGGCCGAGCGGCTCGCGCGCCCCGACCTCGCCGAAGCGGCGCTGGTGTT
CGATCCGGTAATGGTCGCGACGAGCGGGTCGGTGCTCGCCGACGCGGCGACGATCGACGCGTTCCGTGCGCTGCTGCGGC
GCGCGATGGTCGCGACGCCGAACCTGCCCGAACTCGAAGCGCTGGGCGGCGAGGAGGCGGTGCTTGCGCACGGCTGTTCG
CTGCTCGTCAAGGGCGGGCACGGCGCGGGCGCGACGGTGACCGATCGCCTGCTCGAACCGGGCGAGGGCGAGGTTGCGCG
TTGGGAAGCGCCGCGGATCGACACGCCGCACAGCCACGGCACCGGCTGCACGCTGGCGAGCGCGATCGCGTGCGGGCTGG
CGGAGGGAATGCCGCTGGAACCCGCTGTCGCGCGTGCGCGCGATTTCGTGCGGCTGGCGTTGCTCGATGCGCCGGGGCTG
GGGCGAGGGCATGGCCCGATGGGGCAGCAGATGGTGCGCAACGACGGGCTGTTCACGGGGCCGGCGCTCAACCAGATGAC
GTTGCCCGCCGATGATTATGCGGCGTCGGTCGCCTTTTACAAGCAGTTGGGCCTGACGCAGATCGTCGATAGCCCTGACA
ATGGATATGCGCGCTTTGAAGCGGCAAATGGCGTCACGCTGTCGATCCATGTCGGCGATGGCGCGGCGGACGGGGCGACG
ACCTATCTGGAGAGCGGCGCGCTCGATGCGTGGGTCGCCTATCTGGCGCGGCGCGGCGTGCGGTTCGACCAGATGCCGAA
GGACGAGGATTGGGGGTGGCGCGAGGCGCGGCTGACCGATCCGGCGGGGAACCGGCTGTGCCTGTATCAGGCCGGCGAGT
ATCGAAGGTATCCGCCGTGGCGGGTGTAA

Upstream 100 bases:

>100_bases
TTGCGGCGGCGACCTGCTCGACCGGCCGCTGCGCGAGGGCGCGGCGCTGGCGAAGTTTCCGGCCTCGACGGAGCGGCGGC
ATCCGGGGGCCAGAGGTTAG

Downstream 100 bases:

>100_bases
ACTTCGAAACGATATCATCGGTCCGTTCGTGTCGAGCGAAGTCGAGACACCCATCGGCTTGGTGCAACGCTCATGGGCAT
CGCGACTTCGCTCGATGCGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MPTTPRVLIIAGSDSGGGAGIQADIKAVTMLGGHAMTAITAITAQNTLGVQAVHRVPTEMVLAQIDSVAADIGVDAVKIG
MIGSAETAAAVAERLARPDLAEAALVFDPVMVATSGSVLADAATIDAFRALLRRAMVATPNLPELEALGGEEAVLAHGCS
LLVKGGHGAGATVTDRLLEPGEGEVARWEAPRIDTPHSHGTGCTLASAIACGLAEGMPLEPAVARARDFVRLALLDAPGL
GRGHGPMGQQMVRNDGLFTGPALNQMTLPADDYAASVAFYKQLGLTQIVDSPDNGYARFEAANGVTLSIHVGDGAADGAT
TYLESGALDAWVAYLARRGVRFDQMPKDEDWGWREARLTDPAGNRLCLYQAGEYRRYPPWRV

Sequences:

>Translated_382_residues
MPTTPRVLIIAGSDSGGGAGIQADIKAVTMLGGHAMTAITAITAQNTLGVQAVHRVPTEMVLAQIDSVAADIGVDAVKIG
MIGSAETAAAVAERLARPDLAEAALVFDPVMVATSGSVLADAATIDAFRALLRRAMVATPNLPELEALGGEEAVLAHGCS
LLVKGGHGAGATVTDRLLEPGEGEVARWEAPRIDTPHSHGTGCTLASAIACGLAEGMPLEPAVARARDFVRLALLDAPGL
GRGHGPMGQQMVRNDGLFTGPALNQMTLPADDYAASVAFYKQLGLTQIVDSPDNGYARFEAANGVTLSIHVGDGAADGAT
TYLESGALDAWVAYLARRGVRFDQMPKDEDWGWREARLTDPAGNRLCLYQAGEYRRYPPWRV
>Mature_381_residues
PTTPRVLIIAGSDSGGGAGIQADIKAVTMLGGHAMTAITAITAQNTLGVQAVHRVPTEMVLAQIDSVAADIGVDAVKIGM
IGSAETAAAVAERLARPDLAEAALVFDPVMVATSGSVLADAATIDAFRALLRRAMVATPNLPELEALGGEEAVLAHGCSL
LVKGGHGAGATVTDRLLEPGEGEVARWEAPRIDTPHSHGTGCTLASAIACGLAEGMPLEPAVARARDFVRLALLDAPGLG
RGHGPMGQQMVRNDGLFTGPALNQMTLPADDYAASVAFYKQLGLTQIVDSPDNGYARFEAANGVTLSIHVGDGAADGATT
YLESGALDAWVAYLARRGVRFDQMPKDEDWGWREARLTDPAGNRLCLYQAGEYRRYPPWRV

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiaminase-2 family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=253, Percent_Identity=41.1067193675889, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325378, Length=255, Percent_Identity=32.9411764705882, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324517, Length=255, Percent_Identity=36.8627450980392, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324997, Length=253, Percent_Identity=36.7588932806324, Blast_Score=113, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016084
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR004305
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF03070 TENA_THI-4; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 39813; Mature: 39681

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTPRVLIIAGSDSGGGAGIQADIKAVTMLGGHAMTAITAITAQNTLGVQAVHRVPTEM
CCCCCEEEEEECCCCCCCCCEECCEEEEEEECCHHHHHHHHHHCCCCCCHHHHHHCCHHH
VLAQIDSVAADIGVDAVKIGMIGSAETAAAVAERLARPDLAEAALVFDPVMVATSGSVLA
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCEECCCCHHH
DAATIDAFRALLRRAMVATPNLPELEALGGEEAVLAHGCSLLVKGGHGAGATVTDRLLEP
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHCCCEEEEECCCCCCCHHHHHHCCC
GEGEVARWEAPRIDTPHSHGTGCTLASAIACGLAEGMPLEPAVARARDFVRLALLDAPGL
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEECCCCC
GRGHGPMGQQMVRNDGLFTGPALNQMTLPADDYAASVAFYKQLGLTQIVDSPDNGYARFE
CCCCCCCHHHHHHCCCCEECCCCCCEECCCHHHHHHHHHHHHCCHHHHHCCCCCCEEEEE
AANGVTLSIHVGDGAADGATTYLESGALDAWVAYLARRGVRFDQMPKDEDWGWREARLTD
ECCCEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECC
PAGNRLCLYQAGEYRRYPPWRV
CCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PTTPRVLIIAGSDSGGGAGIQADIKAVTMLGGHAMTAITAITAQNTLGVQAVHRVPTEM
CCCCEEEEEECCCCCCCCCEECCEEEEEEECCHHHHHHHHHHCCCCCCHHHHHHCCHHH
VLAQIDSVAADIGVDAVKIGMIGSAETAAAVAERLARPDLAEAALVFDPVMVATSGSVLA
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCEECCCCHHH
DAATIDAFRALLRRAMVATPNLPELEALGGEEAVLAHGCSLLVKGGHGAGATVTDRLLEP
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHCCCEEEEECCCCCCCHHHHHHCCC
GEGEVARWEAPRIDTPHSHGTGCTLASAIACGLAEGMPLEPAVARARDFVRLALLDAPGL
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEECCCCC
GRGHGPMGQQMVRNDGLFTGPALNQMTLPADDYAASVAFYKQLGLTQIVDSPDNGYARFE
CCCCCCCHHHHHHCCCCEECCCCCCEECCCHHHHHHHHHHHHCCHHHHHCCCCCCEEEEE
AANGVTLSIHVGDGAADGATTYLESGALDAWVAYLARRGVRFDQMPKDEDWGWREARLTD
ECCCEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECC
PAGNRLCLYQAGEYRRYPPWRV
CCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]