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The map label for this gene is lmrB [H]

Identifier: 103486388

GI number: 103486388

Start: 906523

End: 907917

Strand: Direct

Name: lmrB [H]

Synonym: Sala_0898

Alternate gene names: 103486388

Gene position: 906523-907917 (Clockwise)

Preceding gene: 103486387

Following gene: 103486389

Centisome position: 27.1

GC content: 65.52

Gene sequence:

>1395_bases
GTGGGGGACCGGCGCAACCTGCCGTTGTTGATCGGCGCCCTCTGGGTCGCGGAAGTCACCGGATCGTTCGAAACGGCGAT
GATCCTTGCCGCGCTCAAGCGGCTGATCGAGGATTTCGGCAATCCGGCGATGGTGGGCTGGCTGATCACCGGCTATCTGA
TCGTCGGCGCGGCGATCGCCGCGGTGGTCGGCCGCCTCGGCGACCTGTTCGGGCGACGCCAGACGCTGATCATTGTATTG
ACGATCGGCGCGATCGGTTCGTTGATCAGCGCCCTGTCAACCAGCTTTCCCGTCCTCTTGGCGGGCCGCCTGATGCAGGG
CGTTACCGGCGCGATCCTGCCGCTCTGTATCGGGCTGGTGCATGAAAACATGTCGAGGGACCGGGCGCCGATGGGTATCG
GACTGATGATTTCCGGTGCATCGATCGGCACGGCGGCGGGGCTTGTCGTCGGCGGAATGATCGTCGACAGCTTTAACTGG
CATGGGATATTCTTTGCCAGCGCGGGCCTGTGTGTGACCGCCGCGCTCGCCACCCGCGCTTTCGTCCCGGCCTCGCCGCG
TCAATATGGCGGCGCCCCGGTGGACTGGATTTCGGGCTTGGCCTTTGCTCCCGGAGTGGCGCTCGTCCTCGTTTACTTCA
GCATGGGCAAGACGTGGGGCTGGTCCAGTGCGTGGACCCTCGGGCCGCTGTTGACGGGCGCCTTGCTGCTGTTCTGGTGG
ATGCGGCACAGCCTTGCCAGCCCCAATCCTCTCATCGCGGTCCGCAGCTTTTCCAATCGTACGATCGCGATCGGCGGGGC
GGTGACGGCGCTGGTGTCGATGGGGACGCTTCAGATCACCGTCTTTTTCTCCCTGCTGATGCAGGCGCCGACCTGGACCG
TCGCCGGGCTGGGATTTTCGGCGACGCTCGCGGGCCTTGCGAAGCTGCCCTCGAACCTCAGCTCGACCTTCGCGGGGCCG
CTCGGCGGCTGGCTCGCGGGGCGCGGCGGCGGCCGTTTCGCGCTGGTTGTCGGCGGCGCGCTCACCACGGCGGGCTGGCT
CGTCTGGTTCCTGCTCGATGTGAACAGCTTTCCGATGGTCGTCGCACAGCTCATCCTGATTTCATTCGGCGCGACGATGC
TTTTTTCGGTCGCGCCGACGATCATCGCACAGGCGTCGCCGCCCGAGCGGATCAGCGAGATTTCGGGAATGCTGACCGTC
ATTCGCCAGCTTTTCCTGGGCATCGGTGCCCAGATGGTCACGACCCTGCTCGCGGTGGATGTCGTGCGCCGCGGGGACGA
GGTCTATCCCTCGCCCTTTTCCTACGATCTCGCCGTCACGGCGATCGTCGCACTGTGCGTCGGCGCGATCCTGATCGCCT
TCCTCCTTCCCCGCTCCATCAGGGAAACGCCATGA

Upstream 100 bases:

>100_bases
GCCGTCATCGGTCTGCCAGCCGACATCGGCGTCGATCTCATCGAGGTGCGCGCCGCACAGGAAGGGGCGCCCGAGGGAGC
AAAGCAGGTAGCCGAATAGG

Downstream 100 bases:

>100_bases
GACCTCTTCCCGAACTCACACCCGAAAACACCGCCTTCTGGACCGGCGGCGCGCAGGGTGAATTGTTGATCGCGCGCTGC
GACGACTGCGATCATGCCAT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MGDRRNLPLLIGALWVAEVTGSFETAMILAALKRLIEDFGNPAMVGWLITGYLIVGAAIAAVVGRLGDLFGRRQTLIIVL
TIGAIGSLISALSTSFPVLLAGRLMQGVTGAILPLCIGLVHENMSRDRAPMGIGLMISGASIGTAAGLVVGGMIVDSFNW
HGIFFASAGLCVTAALATRAFVPASPRQYGGAPVDWISGLAFAPGVALVLVYFSMGKTWGWSSAWTLGPLLTGALLLFWW
MRHSLASPNPLIAVRSFSNRTIAIGGAVTALVSMGTLQITVFFSLLMQAPTWTVAGLGFSATLAGLAKLPSNLSSTFAGP
LGGWLAGRGGGRFALVVGGALTTAGWLVWFLLDVNSFPMVVAQLILISFGATMLFSVAPTIIAQASPPERISEISGMLTV
IRQLFLGIGAQMVTTLLAVDVVRRGDEVYPSPFSYDLAVTAIVALCVGAILIAFLLPRSIRETP

Sequences:

>Translated_464_residues
MGDRRNLPLLIGALWVAEVTGSFETAMILAALKRLIEDFGNPAMVGWLITGYLIVGAAIAAVVGRLGDLFGRRQTLIIVL
TIGAIGSLISALSTSFPVLLAGRLMQGVTGAILPLCIGLVHENMSRDRAPMGIGLMISGASIGTAAGLVVGGMIVDSFNW
HGIFFASAGLCVTAALATRAFVPASPRQYGGAPVDWISGLAFAPGVALVLVYFSMGKTWGWSSAWTLGPLLTGALLLFWW
MRHSLASPNPLIAVRSFSNRTIAIGGAVTALVSMGTLQITVFFSLLMQAPTWTVAGLGFSATLAGLAKLPSNLSSTFAGP
LGGWLAGRGGGRFALVVGGALTTAGWLVWFLLDVNSFPMVVAQLILISFGATMLFSVAPTIIAQASPPERISEISGMLTV
IRQLFLGIGAQMVTTLLAVDVVRRGDEVYPSPFSYDLAVTAIVALCVGAILIAFLLPRSIRETP
>Mature_463_residues
GDRRNLPLLIGALWVAEVTGSFETAMILAALKRLIEDFGNPAMVGWLITGYLIVGAAIAAVVGRLGDLFGRRQTLIIVLT
IGAIGSLISALSTSFPVLLAGRLMQGVTGAILPLCIGLVHENMSRDRAPMGIGLMISGASIGTAAGLVVGGMIVDSFNWH
GIFFASAGLCVTAALATRAFVPASPRQYGGAPVDWISGLAFAPGVALVLVYFSMGKTWGWSSAWTLGPLLTGALLLFWWM
RHSLASPNPLIAVRSFSNRTIAIGGAVTALVSMGTLQITVFFSLLMQAPTWTVAGLGFSATLAGLAKLPSNLSSTFAGPL
GGWLAGRGGGRFALVVGGALTTAGWLVWFLLDVNSFPMVVAQLILISFGATMLFSVAPTIIAQASPPERISEISGMLTVI
RQLFLGIGAQMVTTLLAVDVVRRGDEVYPSPFSYDLAVTAIVALCVGAILIAFLLPRSIRETP

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=414, Percent_Identity=25.3623188405797, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789042, Length=379, Percent_Identity=22.9551451187335, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=310, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=125, Percent_Identity=35.2, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=141, Percent_Identity=31.2056737588652, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48447; Mature: 48316

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDRRNLPLLIGALWVAEVTGSFETAMILAALKRLIEDFGNPAMVGWLITGYLIVGAAIA
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AVVGRLGDLFGRRQTLIIVLTIGAIGSLISALSTSFPVLLAGRLMQGVTGAILPLCIGLV
HHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
HENMSRDRAPMGIGLMISGASIGTAAGLVVGGMIVDSFNWHGIFFASAGLCVTAALATRA
HHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHH
FVPASPRQYGGAPVDWISGLAFAPGVALVLVYFSMGKTWGWSSAWTLGPLLTGALLLFWW
CCCCCCHHCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
MRHSLASPNPLIAVRSFSNRTIAIGGAVTALVSMGTLQITVFFSLLMQAPTWTVAGLGFS
HHHHCCCCCCEEEEEECCCCEEEECHHHHHHHHHCHHHHHHHHHHHHHCCCCEEECCCHH
ATLAGLAKLPSNLSSTFAGPLGGWLAGRGGGRFALVVGGALTTAGWLVWFLLDVNSFPMV
HHHHHHHHCCCHHHHHHCCCCCCHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHH
VAQLILISFGATMLFSVAPTIIAQASPPERISEISGMLTVIRQLFLGIGAQMVTTLLAVD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VVRRGDEVYPSPFSYDLAVTAIVALCVGAILIAFLLPRSIRETP
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure 
GDRRNLPLLIGALWVAEVTGSFETAMILAALKRLIEDFGNPAMVGWLITGYLIVGAAIA
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AVVGRLGDLFGRRQTLIIVLTIGAIGSLISALSTSFPVLLAGRLMQGVTGAILPLCIGLV
HHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
HENMSRDRAPMGIGLMISGASIGTAAGLVVGGMIVDSFNWHGIFFASAGLCVTAALATRA
HHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHH
FVPASPRQYGGAPVDWISGLAFAPGVALVLVYFSMGKTWGWSSAWTLGPLLTGALLLFWW
CCCCCCHHCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
MRHSLASPNPLIAVRSFSNRTIAIGGAVTALVSMGTLQITVFFSLLMQAPTWTVAGLGFS
HHHHCCCCCCEEEEEECCCCEEEECHHHHHHHHHCHHHHHHHHHHHHHCCCCEEECCCHH
ATLAGLAKLPSNLSSTFAGPLGGWLAGRGGGRFALVVGGALTTAGWLVWFLLDVNSFPMV
HHHHHHHHCCCHHHHHHCCCCCCHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHHH
VAQLILISFGATMLFSVAPTIIAQASPPERISEISGMLTVIRQLFLGIGAQMVTTLLAVD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VVRRGDEVYPSPFSYDLAVTAIVALCVGAILIAFLLPRSIRETP
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]