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The map label for this gene is menE [H]

Identifier: 103486371

GI number: 103486371

Start: 886665

End: 888164

Strand: Direct

Name: menE [H]

Synonym: Sala_0881

Alternate gene names: 103486371

Gene position: 886665-888164 (Clockwise)

Preceding gene: 103486370

Following gene: 103486372

Centisome position: 26.51

GC content: 64.27

Gene sequence:

>1500_bases
ATGGCGCTGATGACCGAAAAGCTTCGTGCCATCATGGCGCTCGACCCCGACCGGAGCGAGATTGATTTCGAGGGAGTCGA
TTATAGCTGGCGGCAGATCGCCGACGCGGTGCGCGCCATTGAAACGGCGCTCGCCGATATGGGCCTGCCCGCCGATGCAC
GCGTCGGCGTCCTGCTTCGCAACCGGCCGGGCCATGTCGCGGCGATCGTGGCAGTGCTTTCGACCGACCGTTGCCTGGTA
ACATTGAACCCCATTCTACCCGACGCGCGCCTGTTCGCCGATGTCGAGGGGCTGGCGTTGCCGGCCGTCATCGCCGATGC
GAGCGATCTGGCGCGACCCGGCCTTGCCGGGGCGCTAACGAACGCGGGGTCGGCGGTGATCGAGATCGGACCGCGGCTGG
AGGGCGTCCGTGTGGTGCAGCAAAAGCTGCGGCACGCCGACCAAGTCTCGCCCGGCGTCGCCATCGAAATGCTGACCAGC
GGCACTACCGGCCCGCCCAAGCGCGTGCCGCTCAGCCGCGATGCCTTCGACGCCAGCTTTCGTGGATTCATGCGCTATGA
ACGGGGGCGCAGCTTCGACGATCCACCGCGGCTGAGCTCGGGCTGCACCATGATCGTCAATCCGCTGACCCACATCGGGG
GTATTTACGGCTGCATCGGCGCCCTTGCGGCCGGGCGAAAAATTGCGCTGCTCGAAAAGTTCAGCGTCGAGGCGTGGGTT
GGCGCGGTGAAGCGCAACCGCCCGGCGGTCGCGCCCGCGGTTCCATCGGCGGTGCGGATGCTGCTCGACGCCGATCTCGA
CCCCGACGATCTGTCGAGCCTCAAGTCACTGATTTCCGGTACTGCGCCGCTGTCACCCGATCTGGTCGACGCCTTCCTTG
CCAAATATGGCATCCCCATCTGCGCCAACTATGGTGCGACCGAGTTCGCTGGCGCCGTGGCCGGGTGGAATATCGACGAT
TTCCGGAAGTTCTGGACTGAAAAGCGTGGTTCCGTGGGGCGCATCCACGCCGATATTGAAGCGCGCGTCGTGGATGCCGA
CAGCGGTGCGGAACTGCCCGCCGGTCGGGAAGGGCTGCTCGAACTCAAGGGGCAGCAGCTCAACAATAACATGCAATGGC
TCCGCACGACCGATCGTGCCGTGCTGGACGCTGATGGCTTTCTTTTCATCAAGGGGCGGGCCGACAATGCGATCATTCGC
GGCGGGTTCAAAATCCACCCGGATGATGTTGTTCAGGTGCTCAACATGCATCCTTCTGTTCGCGAATCCGCCGTGGTCGG
AATCGACGACGATCGGTTGGGCGCCGTACCCGCCGCAGCGATCATCCTGAAGGATGGAACCGATGTGCCGACCACCGCCG
ACCTGAAGGCGTGGCTGAAGGAACGCCTGATTGCCTATCAGGTGCCGACCCGCATCCAGATCGTCACCGACTTTCCGCGC
ACGCCGTCCATGAAGCCCTCGGCACCGGGCCTGCGCGCCCTTTTTGCGGAGGATGAATGA

Upstream 100 bases:

>100_bases
AACCCGACGATATCGCGGGCGCGGTGATGATGTTGCTGGGCGACGATGCGCGCTGGGTGACCGGACAGGTGATCGAAGCC
AGCGGGGGCCTCGCCCTCTG

Downstream 100 bases:

>100_bases
TGGAAGATTTTTCGGGCAAGGTGGTAATCGTCACCGGCGGCGGACGCGGCGTAGGGCGCGGTGTCGCAAAGGCTTTCGCG
GCGGCGGGCGCCAAGGTCAT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MALMTEKLRAIMALDPDRSEIDFEGVDYSWRQIADAVRAIETALADMGLPADARVGVLLRNRPGHVAAIVAVLSTDRCLV
TLNPILPDARLFADVEGLALPAVIADASDLARPGLAGALTNAGSAVIEIGPRLEGVRVVQQKLRHADQVSPGVAIEMLTS
GTTGPPKRVPLSRDAFDASFRGFMRYERGRSFDDPPRLSSGCTMIVNPLTHIGGIYGCIGALAAGRKIALLEKFSVEAWV
GAVKRNRPAVAPAVPSAVRMLLDADLDPDDLSSLKSLISGTAPLSPDLVDAFLAKYGIPICANYGATEFAGAVAGWNIDD
FRKFWTEKRGSVGRIHADIEARVVDADSGAELPAGREGLLELKGQQLNNNMQWLRTTDRAVLDADGFLFIKGRADNAIIR
GGFKIHPDDVVQVLNMHPSVRESAVVGIDDDRLGAVPAAAIILKDGTDVPTTADLKAWLKERLIAYQVPTRIQIVTDFPR
TPSMKPSAPGLRALFAEDE

Sequences:

>Translated_499_residues
MALMTEKLRAIMALDPDRSEIDFEGVDYSWRQIADAVRAIETALADMGLPADARVGVLLRNRPGHVAAIVAVLSTDRCLV
TLNPILPDARLFADVEGLALPAVIADASDLARPGLAGALTNAGSAVIEIGPRLEGVRVVQQKLRHADQVSPGVAIEMLTS
GTTGPPKRVPLSRDAFDASFRGFMRYERGRSFDDPPRLSSGCTMIVNPLTHIGGIYGCIGALAAGRKIALLEKFSVEAWV
GAVKRNRPAVAPAVPSAVRMLLDADLDPDDLSSLKSLISGTAPLSPDLVDAFLAKYGIPICANYGATEFAGAVAGWNIDD
FRKFWTEKRGSVGRIHADIEARVVDADSGAELPAGREGLLELKGQQLNNNMQWLRTTDRAVLDADGFLFIKGRADNAIIR
GGFKIHPDDVVQVLNMHPSVRESAVVGIDDDRLGAVPAAAIILKDGTDVPTTADLKAWLKERLIAYQVPTRIQIVTDFPR
TPSMKPSAPGLRALFAEDE
>Mature_498_residues
ALMTEKLRAIMALDPDRSEIDFEGVDYSWRQIADAVRAIETALADMGLPADARVGVLLRNRPGHVAAIVAVLSTDRCLVT
LNPILPDARLFADVEGLALPAVIADASDLARPGLAGALTNAGSAVIEIGPRLEGVRVVQQKLRHADQVSPGVAIEMLTSG
TTGPPKRVPLSRDAFDASFRGFMRYERGRSFDDPPRLSSGCTMIVNPLTHIGGIYGCIGALAAGRKIALLEKFSVEAWVG
AVKRNRPAVAPAVPSAVRMLLDADLDPDDLSSLKSLISGTAPLSPDLVDAFLAKYGIPICANYGATEFAGAVAGWNIDDF
RKFWTEKRGSVGRIHADIEARVVDADSGAELPAGREGLLELKGQQLNNNMQWLRTTDRAVLDADGFLFIKGRADNAIIRG
GFKIHPDDVVQVLNMHPSVRESAVVGIDDDRLGAVPAAAIILKDGTDVPTTADLKAWLKERLIAYQVPTRIQIVTDFPRT
PSMKPSAPGLRALFAEDE

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=353, Percent_Identity=28.8951841359773, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI42544132, Length=514, Percent_Identity=23.5408560311284, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI122937307, Length=249, Percent_Identity=28.5140562248996, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI38505220, Length=364, Percent_Identity=26.6483516483516, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI157311624, Length=370, Percent_Identity=25.6756756756757, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI157311622, Length=370, Percent_Identity=25.6756756756757, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI58082049, Length=364, Percent_Identity=25, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI115511026, Length=363, Percent_Identity=24.2424242424242, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI187761345, Length=377, Percent_Identity=24.4031830238727, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI187761343, Length=377, Percent_Identity=24.4031830238727, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI145693145, Length=359, Percent_Identity=27.5766016713092, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI221142682, Length=349, Percent_Identity=26.647564469914, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786810, Length=365, Percent_Identity=26.5753424657534, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788107, Length=351, Percent_Identity=25.3561253561254, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788595, Length=331, Percent_Identity=28.0966767371601, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790505, Length=375, Percent_Identity=25.3333333333333, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559526, Length=347, Percent_Identity=25.9365994236311, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=557, Percent_Identity=24.0574506283662, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71994690, Length=557, Percent_Identity=24.0574506283662, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=371, Percent_Identity=25.8760107816712, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71994694, Length=557, Percent_Identity=24.0574506283662, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=362, Percent_Identity=25.1381215469613, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=313, Percent_Identity=25.8785942492013, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=335, Percent_Identity=25.0746268656716, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=344, Percent_Identity=26.7441860465116, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=496, Percent_Identity=22.9838709677419, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17560308, Length=356, Percent_Identity=26.9662921348315, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=499, Percent_Identity=20.8416833667335, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=378, Percent_Identity=25.9259259259259, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319264, Length=384, Percent_Identity=25.78125, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323182, Length=380, Percent_Identity=26.8421052631579, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24581924, Length=366, Percent_Identity=28.9617486338798, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21355181, Length=452, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859661, Length=502, Percent_Identity=25.6972111553785, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648257, Length=449, Percent_Identity=24.9443207126949, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19922652, Length=517, Percent_Identity=22.2437137330754, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356441, Length=361, Percent_Identity=24.3767313019391, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356947, Length=506, Percent_Identity=22.7272727272727, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 53483; Mature: 53352

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALMTEKLRAIMALDPDRSEIDFEGVDYSWRQIADAVRAIETALADMGLPADARVGVLLR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
NRPGHVAAIVAVLSTDRCLVTLNPILPDARLFADVEGLALPAVIADASDLARPGLAGALT
CCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCCHHHHHHCHHHHCCCCCHHHHH
NAGSAVIEIGPRLEGVRVVQQKLRHADQVSPGVAIEMLTSGTTGPPKRVPLSRDAFDASF
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
RGFMRYERGRSFDDPPRLSSGCTMIVNPLTHIGGIYGCIGALAAGRKIALLEKFSVEAWV
HHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHH
GAVKRNRPAVAPAVPSAVRMLLDADLDPDDLSSLKSLISGTAPLSPDLVDAFLAKYGIPI
HHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCE
CANYGATEFAGAVAGWNIDDFRKFWTEKRGSVGRIHADIEARVVDADSGAELPAGREGLL
EECCCCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECEEEEEEECCCCCCCCCCCCCHH
ELKGQQLNNNMQWLRTTDRAVLDADGFLFIKGRADNAIIRGGFKIHPDDVVQVLNMHPSV
HHCCHHHCCCCCEEEECCCEEEECCCEEEEEECCCCEEEECCCEECHHHHHHHHHCCCCC
RESAVVGIDDDRLGAVPAAAIILKDGTDVPTTADLKAWLKERLIAYQVPTRIQIVTDFPR
CCCEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHEEECCCEEEEEECCCC
TPSMKPSAPGLRALFAEDE
CCCCCCCCCCCEEEECCCC
>Mature Secondary Structure 
ALMTEKLRAIMALDPDRSEIDFEGVDYSWRQIADAVRAIETALADMGLPADARVGVLLR
CCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
NRPGHVAAIVAVLSTDRCLVTLNPILPDARLFADVEGLALPAVIADASDLARPGLAGALT
CCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCCHHHHHHCHHHHCCCCCHHHHH
NAGSAVIEIGPRLEGVRVVQQKLRHADQVSPGVAIEMLTSGTTGPPKRVPLSRDAFDASF
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
RGFMRYERGRSFDDPPRLSSGCTMIVNPLTHIGGIYGCIGALAAGRKIALLEKFSVEAWV
HHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHH
GAVKRNRPAVAPAVPSAVRMLLDADLDPDDLSSLKSLISGTAPLSPDLVDAFLAKYGIPI
HHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCE
CANYGATEFAGAVAGWNIDDFRKFWTEKRGSVGRIHADIEARVVDADSGAELPAGREGLL
EECCCCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECEEEEEEECCCCCCCCCCCCCHH
ELKGQQLNNNMQWLRTTDRAVLDADGFLFIKGRADNAIIRGGFKIHPDDVVQVLNMHPSV
HHCCHHHCCCCCEEEECCCEEEECCCEEEEEECCCCEEEECCCEECHHHHHHHHHCCCCC
RESAVVGIDDDRLGAVPAAAIILKDGTDVPTTADLKAWLKERLIAYQVPTRIQIVTDFPR
CCCEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHEEECCCEEEEEECCCC
TPSMKPSAPGLRALFAEDE
CCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA