BacMap

Definition	Sphingopyxis alaskensis RB2256, complete genome.
Accession	NC_008048
Length	3,345,170

Click here to switch to the map view.

The map label for this gene is paaG [C]

Identifier: 103486343

GI number: 103486343

Start: 860647

End: 861444

Strand: Direct

Name: paaG [C]

Synonym: Sala_0853

Alternate gene names: 103486343

Gene position: 860647-861444 (Clockwise)

Preceding gene: 103486342

Following gene: 103486344

Centisome position: 25.73

GC content: 65.04

Gene sequence:

>798_bases
GTGGCTGAACCCGACCATATTCGCATCGAACGCCGCGAGCATGGAATCGCAGCCGTTGTGGTGGATCGCGCCGACATCGG
CAATGCCTCCTCGCCCGAAATGCTGGGCGAAATTCGCGACGCTTTTACGGCGCTTTCGGCCGACCGGGAGGTTCGCGCGA
TCATCCTCGCGGCCGAGGGAAAGCATTTTTCGGTCGGGGCCGATTTTGCCTTTCTGGGGCGCCTGACCAGCATGGCAGCG
ACCGAGATCAAGGACACGGTTTACGCCAATTTCCAGGGCGCCGCGCGCGCCATCTATCGTTCGCCCAAACCGACGCTCGC
CGTGGTTCAGGGCGCCGCCGTCACTGTGGGGTGCGAGCTTGCGCTCGCCTGCGATTTTCGGATCGCCGCCGACAATGCGA
TGTTCCAGGAAAGCTGGATCAAGCTGGGCATCATGCCCCCGCTTGGCGGAACTTTCCTGCTGCCCCGCATCGTCGGGCTG
GGCCGCGCGATGGATATGTGCCTGCGCGGTCGGCAGGTGCGCGCCGAAGAGGCCTTGGCCATCGGACTGGTCGCCGAGGT
GGTGGCAAGGGACGATCTGGGGGAACGCGGCATGGCGTTGGCGCGCGAACTGGCTGCCGCCGCTCCGCTGGGCTATGCGA
CGGTGAAGCAGTCGCTTCAACGCGCGCTCGAAAGCAGCATGGACGCGGAATGGCAGGCGAATCTGTCCAACCAGGCGTTG
CTGCTTGGCAGCGAAGATCATCGCGAGGGGCTGGCGGCTGTAACCGAAAAGCGCGCGCCGCGTTTTCGCGGCGCATAG

Upstream 100 bases:

>100_bases
TTCGAAGCCGCGCTTGATGCCGAAGCATTGAACATGGGGCTTTCGACCGGCGCAACCGCGTTGATCTGGCGCGAAAAGAA
AAAGCGGGAAGAACAGGGCG

Downstream 100 bases:

>100_bases
CGCCCGCGCTAGAGCGGCGTGCAACAGCATCGGCAAACCAGGGTGCGGGCAAGCCCGCCCGGTTTGACCCCCCCCTTCGG
CCTCGGTGAAATAATGCGGT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MAEPDHIRIERREHGIAAVVVDRADIGNASSPEMLGEIRDAFTALSADREVRAIILAAEGKHFSVGADFAFLGRLTSMAA
TEIKDTVYANFQGAARAIYRSPKPTLAVVQGAAVTVGCELALACDFRIAADNAMFQESWIKLGIMPPLGGTFLLPRIVGL
GRAMDMCLRGRQVRAEEALAIGLVAEVVARDDLGERGMALARELAAAAPLGYATVKQSLQRALESSMDAEWQANLSNQAL
LLGSEDHREGLAAVTEKRAPRFRGA

Sequences:

>Translated_265_residues
MAEPDHIRIERREHGIAAVVVDRADIGNASSPEMLGEIRDAFTALSADREVRAIILAAEGKHFSVGADFAFLGRLTSMAA
TEIKDTVYANFQGAARAIYRSPKPTLAVVQGAAVTVGCELALACDFRIAADNAMFQESWIKLGIMPPLGGTFLLPRIVGL
GRAMDMCLRGRQVRAEEALAIGLVAEVVARDDLGERGMALARELAAAAPLGYATVKQSLQRALESSMDAEWQANLSNQAL
LLGSEDHREGLAAVTEKRAPRFRGA
>Mature_264_residues
AEPDHIRIERREHGIAAVVVDRADIGNASSPEMLGEIRDAFTALSADREVRAIILAAEGKHFSVGADFAFLGRLTSMAAT
EIKDTVYANFQGAARAIYRSPKPTLAVVQGAAVTVGCELALACDFRIAADNAMFQESWIKLGIMPPLGGTFLLPRIVGLG
RAMDMCLRGRQVRAEEALAIGLVAEVVARDDLGERGMALARELAAAAPLGYATVKQSLQRALESSMDAEWQANLSNQALL
LGSEDHREGLAAVTEKRAPRFRGA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=28.3524904214559, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI4502327, Length=272, Percent_Identity=31.25, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=29.9610894941634, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI70995211, Length=269, Percent_Identity=25.2788104089219, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI68989263, Length=185, Percent_Identity=34.0540540540541, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI221307494, Length=228, Percent_Identity=28.0701754385965, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI221136756, Length=230, Percent_Identity=27.8260869565217, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI221136753, Length=230, Percent_Identity=27.8260869565217, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI116235487, Length=203, Percent_Identity=26.6009852216749, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4757966, Length=205, Percent_Identity=28.2926829268293, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI51538581, Length=205, Percent_Identity=28.2926829268293, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI25453481, Length=205, Percent_Identity=28.2926829268293, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI51538573, Length=205, Percent_Identity=28.2926829268293, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI4757968, Length=201, Percent_Identity=27.8606965174129, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI49355787, Length=201, Percent_Identity=27.8606965174129, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI20127408, Length=207, Percent_Identity=30.9178743961353, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=181, Percent_Identity=30.939226519337, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI260275230, Length=181, Percent_Identity=30.939226519337, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI260274832, Length=181, Percent_Identity=30.939226519337, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI157694516, Length=238, Percent_Identity=26.0504201680672, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI213417737, Length=238, Percent_Identity=26.0504201680672, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI157694520, Length=211, Percent_Identity=26.5402843601896, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=31.7460317460317, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=32.4324324324324, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI221142681, Length=271, Percent_Identity=34.3173431734317, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=30.9160305343511, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788682, Length=239, Percent_Identity=28.8702928870293, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790281, Length=203, Percent_Identity=30.5418719211823, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082183, Length=242, Percent_Identity=28.5123966942149, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=31.7460317460317, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17554946, Length=261, Percent_Identity=28.735632183908, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=231, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=273, Percent_Identity=25.6410256410256, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=245, Percent_Identity=26.9387755102041, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=237, Percent_Identity=25.7383966244726, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=188, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=250, Percent_Identity=24.4, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=243, Percent_Identity=27.5720164609054, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=243, Percent_Identity=27.5720164609054, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=243, Percent_Identity=27.5720164609054, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=180, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=246, Percent_Identity=28.4552845528455, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=247, Percent_Identity=27.1255060728745, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=30, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=30, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=30.2325581395349, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=28.6259541984733, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=22.6923076923077, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357171, Length=188, Percent_Identity=29.2553191489362, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921018, Length=211, Percent_Identity=26.0663507109005, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19921000, Length=191, Percent_Identity=30.8900523560209, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583077, Length=191, Percent_Identity=30.8900523560209, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583079, Length=191, Percent_Identity=30.8900523560209, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28371; Mature: 28240

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPDHIRIERREHGIAAVVVDRADIGNASSPEMLGEIRDAFTALSADREVRAIILAAEG
CCCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECC
KHFSVGADFAFLGRLTSMAATEIKDTVYANFQGAARAIYRSPKPTLAVVQGAAVTVGCEL
CEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCEEEEEEEE
ALACDFRIAADNAMFQESWIKLGIMPPLGGTFLLPRIVGLGRAMDMCLRGRQVRAEEALA
EEEEEEEEECCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IGLVAEVVARDDLGERGMALARELAAAAPLGYATVKQSLQRALESSMDAEWQANLSNQAL
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCEE
LLGSEDHREGLAAVTEKRAPRFRGA
EECCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AEPDHIRIERREHGIAAVVVDRADIGNASSPEMLGEIRDAFTALSADREVRAIILAAEG
CCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECC
KHFSVGADFAFLGRLTSMAATEIKDTVYANFQGAARAIYRSPKPTLAVVQGAAVTVGCEL
CEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCEEEEEEEE
ALACDFRIAADNAMFQESWIKLGIMPPLGGTFLLPRIVGLGRAMDMCLRGRQVRAEEALA
EEEEEEEEECCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IGLVAEVVARDDLGERGMALARELAAAAPLGYATVKQSLQRALESSMDAEWQANLSNQAL
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCEE
LLGSEDHREGLAAVTEKRAPRFRGA
EECCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA