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The map label for this gene is gatB [H]

Identifier: 103486332

GI number: 103486332

Start: 848780

End: 850255

Strand: Direct

Name: gatB [H]

Synonym: Sala_0842

Alternate gene names: 103486332

Gene position: 848780-850255 (Clockwise)

Preceding gene: 103486331

Following gene: 103486333

Centisome position: 25.37

GC content: 64.23

Gene sequence:

>1476_bases
ATGCGGATGATGAGTGATTATCGAGTGCAGGGCGAAACCGGCGAGTGGGAGGTCGTGATTGGCCTCGAGGTCCACGCCCA
GATCACGACCAACGCCAAGCTGTTTTCGGGCGCCGCGACGGCGTTTGGCGCCGAGCCGAACACGCAGGTGTCGCTGGTCG
ACGCTGCGATGCCGGGGATGCTGCCGGTGCCGAACCGCGAGTGCATCCGCCAGGCGGTGCGCACGGGGATGGCGATCGAG
GCGCAGATCAATCGCTGGTCGCGGTTCGACCGCAAGAATTATTTCTATGCCGACCTGCCGCAGGGCTATCAGATTTCGCA
GCTTTATCATCCGCTGGTCGGCGAAGGTTCGCTGACGATCGCCGCCGATGAAAAGGCCGGGATTCCCGCCGACAAGGTGA
TCGGGATCGAGCGTATCCATGTCGAACAGGATGCGGGGAAGCTGATGCACGACCAGCATCCGACGATGTCCTATGTCGAT
TTGAACCGCAGCGGGGTCGCGTTGATGGAGATCGTGTCGAAACCCGACATGCGCTCGCCCGCCGAAGCCGGCGCCTATGT
GCGCAAGCTGCGCTCGATCCTGCGCTATGTCGGCTCGTGCGACGGCAATATGGAAGAAGGCTCGATGCGCGCCGACGTCA
ACGTCAGCGTGCGGCGGCCCGGCGAGGCGTTCGGCACGCGCACCGAGACGAAGAACGTCAATTCGGTGCGCTTCGTGATG
GCGGTGATCGAGGGCGAGGCGAAGCGGCAGGTCGCGCTGATCGAGAGCGGCGGCGAGGTGGTGCAGGAAACGCGCCTCTA
CGATCCCGACCGGGGCGAGACGCGGTCGATGCGGTCGAAGGAAGACGCGCATGACTATCGCTACTTCCCCGATCCCGACC
TGTTGCCGCTCGAACTCGACGACGCCTTTCTCGAAGAATGCCGCGCGAGCCTGCCTGAATTGCCCGACGCCAAGCGCGCG
CGTTATCTGGCGGCGGGGATCAGCGCCTATAACGCCGATGTGCTGACCGCCGAGGTCGAGGCGGCGCGCTGGTTCGACGC
GCTGCTCGAAGCCGGCGCAAAGCCCGTCGCGGCGGCGAACTGGGTGACGAGCGAGCTGTTCGGCGCCTTGAACCGGCTCG
GTCGCGACATTTCGGATTCGCCAGTCAGTCCGGCGCAGGCCGCCGAATTGCTCGGCCTCGTCGCCGATGGCACGATTTCG
GGGACGATTGCGAAACAGGTATTCGAAAAGATGCTCGAAAGCGGCGACGGCGCCGCGGCGATCGTCGATCGTGAGGGCCT
CAAGCAGACGAGCGACACGGGCGCAATCGAAGCCGAGATCGCCAAGGTTCTCGCCGCCAATGCCGACAAGGTCGAGCAGT
ATAAGGGCGGCAAGGAAGCCCTGTTCGGCTTCTTCGTCGGGCAGACGATGAAGGCGATGCAGGGCAAGGCAAACCCGCAG
GTCGTCAACGAGCTGCTGAAAAAGGCACTGGGCTGA

Upstream 100 bases:

>100_bases
GTGGTGGTGATTTGGTGGTGTGCTCCTGCGAACGCAGGAGCCCAGGGCGGCCTAGCGCTGCCCCACTCTGGGTTCCCGCT
TTCGCGGGAACGCACAAAGG

Downstream 100 bases:

>100_bases
GCCTAAATCTGCTGCGGCGGCACTTCGTCGGGCCCGCGGCCCGGCTCGTCGATGTCACCGCCGCCGGGGCGACCGGGGAT
TTCGGCGGGCTGGCCCGCGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MRMMSDYRVQGETGEWEVVIGLEVHAQITTNAKLFSGAATAFGAEPNTQVSLVDAAMPGMLPVPNRECIRQAVRTGMAIE
AQINRWSRFDRKNYFYADLPQGYQISQLYHPLVGEGSLTIAADEKAGIPADKVIGIERIHVEQDAGKLMHDQHPTMSYVD
LNRSGVALMEIVSKPDMRSPAEAGAYVRKLRSILRYVGSCDGNMEEGSMRADVNVSVRRPGEAFGTRTETKNVNSVRFVM
AVIEGEAKRQVALIESGGEVVQETRLYDPDRGETRSMRSKEDAHDYRYFPDPDLLPLELDDAFLEECRASLPELPDAKRA
RYLAAGISAYNADVLTAEVEAARWFDALLEAGAKPVAAANWVTSELFGALNRLGRDISDSPVSPAQAAELLGLVADGTIS
GTIAKQVFEKMLESGDGAAAIVDREGLKQTSDTGAIEAEIAKVLAANADKVEQYKGGKEALFGFFVGQTMKAMQGKANPQ
VVNELLKKALG

Sequences:

>Translated_491_residues
MRMMSDYRVQGETGEWEVVIGLEVHAQITTNAKLFSGAATAFGAEPNTQVSLVDAAMPGMLPVPNRECIRQAVRTGMAIE
AQINRWSRFDRKNYFYADLPQGYQISQLYHPLVGEGSLTIAADEKAGIPADKVIGIERIHVEQDAGKLMHDQHPTMSYVD
LNRSGVALMEIVSKPDMRSPAEAGAYVRKLRSILRYVGSCDGNMEEGSMRADVNVSVRRPGEAFGTRTETKNVNSVRFVM
AVIEGEAKRQVALIESGGEVVQETRLYDPDRGETRSMRSKEDAHDYRYFPDPDLLPLELDDAFLEECRASLPELPDAKRA
RYLAAGISAYNADVLTAEVEAARWFDALLEAGAKPVAAANWVTSELFGALNRLGRDISDSPVSPAQAAELLGLVADGTIS
GTIAKQVFEKMLESGDGAAAIVDREGLKQTSDTGAIEAEIAKVLAANADKVEQYKGGKEALFGFFVGQTMKAMQGKANPQ
VVNELLKKALG
>Mature_491_residues
MRMMSDYRVQGETGEWEVVIGLEVHAQITTNAKLFSGAATAFGAEPNTQVSLVDAAMPGMLPVPNRECIRQAVRTGMAIE
AQINRWSRFDRKNYFYADLPQGYQISQLYHPLVGEGSLTIAADEKAGIPADKVIGIERIHVEQDAGKLMHDQHPTMSYVD
LNRSGVALMEIVSKPDMRSPAEAGAYVRKLRSILRYVGSCDGNMEEGSMRADVNVSVRRPGEAFGTRTETKNVNSVRFVM
AVIEGEAKRQVALIESGGEVVQETRLYDPDRGETRSMRSKEDAHDYRYFPDPDLLPLELDDAFLEECRASLPELPDAKRA
RYLAAGISAYNADVLTAEVEAARWFDALLEAGAKPVAAANWVTSELFGALNRLGRDISDSPVSPAQAAELLGLVADGTIS
GTIAKQVFEKMLESGDGAAAIVDREGLKQTSDTGAIEAEIAKVLAANADKVEQYKGGKEALFGFFVGQTMKAMQGKANPQ
VVNELLKKALG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=494, Percent_Identity=40.8906882591093, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI115534094, Length=298, Percent_Identity=40.6040268456376, Blast_Score=222, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=519, Percent_Identity=31.9845857418112, Blast_Score=236, Evalue=6e-63,
Organism=Drosophila melanogaster, GI45550808, Length=495, Percent_Identity=35.3535353535354, Blast_Score=296, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53422; Mature: 53422

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMMSDYRVQGETGEWEVVIGLEVHAQITTNAKLFSGAATAFGAEPNTQVSLVDAAMPGM
CCCCCCCEEECCCCCEEEEEEEEEEEEEECCCHHHCCCHHHCCCCCCCEEEEEECCCCCC
LPVPNRECIRQAVRTGMAIEAQINRWSRFDRKNYFYADLPQGYQISQLYHPLVGEGSLTI
CCCCCHHHHHHHHHCCCEEEEHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEE
AADEKAGIPADKVIGIERIHVEQDAGKLMHDQHPTMSYVDLNRSGVALMEIVSKPDMRSP
EECCCCCCCHHHEECEEEEEEECHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCCCC
AEAGAYVRKLRSILRYVGSCDGNMEEGSMRADVNVSVRRPGEAFGTRTETKNVNSVRFVM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECEEEEEECCCHHHCCCCCCCCCCHHHHHH
AVIEGEAKRQVALIESGGEVVQETRLYDPDRGETRSMRSKEDAHDYRYFPDPDLLPLELD
HHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHCCCCHHHCCCCCCCCCCEEEECC
DAFLEECRASLPELPDAKRARYLAAGISAYNADVLTAEVEAARWFDALLEAGAKPVAAAN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCHHHH
WVTSELFGALNRLGRDISDSPVSPAQAAELLGLVADGTISGTIAKQVFEKMLESGDGAAA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEE
IVDREGLKQTSDTGAIEAEIAKVLAANADKVEQYKGGKEALFGFFVGQTMKAMQGKANPQ
EEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
VVNELLKKALG
HHHHHHHHHCC
>Mature Secondary Structure
MRMMSDYRVQGETGEWEVVIGLEVHAQITTNAKLFSGAATAFGAEPNTQVSLVDAAMPGM
CCCCCCCEEECCCCCEEEEEEEEEEEEEECCCHHHCCCHHHCCCCCCCEEEEEECCCCCC
LPVPNRECIRQAVRTGMAIEAQINRWSRFDRKNYFYADLPQGYQISQLYHPLVGEGSLTI
CCCCCHHHHHHHHHCCCEEEEHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEE
AADEKAGIPADKVIGIERIHVEQDAGKLMHDQHPTMSYVDLNRSGVALMEIVSKPDMRSP
EECCCCCCCHHHEECEEEEEEECHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCCCC
AEAGAYVRKLRSILRYVGSCDGNMEEGSMRADVNVSVRRPGEAFGTRTETKNVNSVRFVM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECEEEEEECCCHHHCCCCCCCCCCHHHHHH
AVIEGEAKRQVALIESGGEVVQETRLYDPDRGETRSMRSKEDAHDYRYFPDPDLLPLELD
HHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHCCCCHHHCCCCCCCCCCEEEECC
DAFLEECRASLPELPDAKRARYLAAGISAYNADVLTAEVEAARWFDALLEAGAKPVAAAN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCHHHH
WVTSELFGALNRLGRDISDSPVSPAQAAELLGLVADGTISGTIAKQVFEKMLESGDGAAA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEE
IVDREGLKQTSDTGAIEAEIAKVLAANADKVEQYKGGKEALFGFFVGQTMKAMQGKANPQ
EEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
VVNELLKKALG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA