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The map label for this gene is gatA

Identifier: 103486331

GI number: 103486331

Start: 847208

End: 848689

Strand: Direct

Name: gatA

Synonym: Sala_0841

Alternate gene names: 103486331

Gene position: 847208-848689 (Clockwise)

Preceding gene: 103486330

Following gene: 103486332

Centisome position: 25.33

GC content: 67.48

Gene sequence:

>1482_bases
ATGACCGAGCTGACCAATTTGACCGTCGCGCAGATCCGCGACGGGCACCGCGCGGGCGATTTTTCCGCCGTTGAAGTGGC
CGAGGCGTTCAACGCCAATGTCGCAGCGGCGAAGGCGCTCAATGCGTTTATCGTCGAGACGCCCGATCTTGCGATCGAAG
CCGCGAAGGCGGCCGATGCCGACCGCGCGGCGGGGACGCTGAAACCGCTGTCGGGCGTGCCGATCGGCATGAAGGACCTG
TTCTGCACCAACGGCGTGCAGACGACGGCCGCGAGCCACATGCTCGAAGGCTTCGTGCCGCGCTATGAATCGACCGTCAG
CCAGAAATTGTGGGACGCGGGCGCGGGGATGCTGGGCAAGCTCAACCTCGACCAGTTCGCGATGGGGTCGTCGAACGAGA
CGAGCTATTTCGGCAACGTCATCAGCCCGTGGCGGCGCAACGACGGCGGCAATGCCGCGCTCGCGCCGGGCGGCTCGTCG
GGCGGCTCGTCGGCGGCGATCGCGGCGCGGCTGTGCCCCGCGGCGACGGGGACCGACACCGGCGGGTCGATCCGCCAGCC
CGCGGCCTTCACCGGCATTTCAGGGATCAAGCCGACCTATGGCCGCTGTTCGCGCTGGGGCATCGTGGCGTTCGCCAGCT
CGCTCGACCAGGCGGGGCCGATGGCGCGGACGGTGCGCGACTGCGCGATCATGCTCGAAAATATGGCGGGCTTCGATCCG
AAGGACGCGACCAGCCTCGATCTGCCGGTGCCGAATTGGGAAGCGGCCTTGTCGAGCGATTTGAGGGGCAAGACGGTTGG
TATTCCCAGGGAATATCGGCTGGAGGGTATCGACCCCGACATCGACGCGATGTGGGACGCCGGGATCGCGATGCTGAAGG
ATGCGGAGGCCGCGGTCGTCGAGATCAGCCTGCCGCATACCAAATATGCGCTGCCCGCCTATTATATCATCGCGCCCGCC
GAGGCGTCGTCGAACCTCGCGCGCTATGACGGCGTGCGCTATGGCCTCCGCGATCTGCCGCAGGGCGCGGGGTTGCAGGA
CATGTACGCCGCGACGCGCGCCGAGGGCTTCGGGGCGGAGGTCAAGCGCCGCATCATGATCGGCACCTATGTTCTGTCGG
CGGGCTTCTACGACGCCTATTATACGCAGGCGCAGAAGGTGCGGACGCTGATCGCGCGCGATTTCGAGGCGGCTTTTGGC
TCGTGCGACGTGATCCTCGCGCCGACCGCGCCGTCGGCGGCGTTCGGGCTGGGCGAGAAGATGGCCGATCCGCTGGCGAT
GTATTTGAACGACGTGTTCGCGGTGCCCGCGAGCCTCGCGGGGCTGCCCGCGATGTCGGTCCCCGCGGCGCTGAACCGCG
AAGGGCTGCCGCTGGGTTTGCAGATCATCGGCAAGCCGTTCGACGAGCAGGGGGTGCTGAACGCCGGGCTGGCGATTGAA
GAGCGCGCCGGGTTCACGGCGCGGGCGGAGAAGTGGTGGTGA

Upstream 100 bases:

>100_bases
ACGCCGACCCGCTGACCGGCGGCAACCGCCGCGACGATATTCTCGCCAATGCGCCGGCGGCGATGCACGGCTTTTTCGGC
GTGCCGAAAGTGATCGAATA

Downstream 100 bases:

>100_bases
TTTGGTGGTGTGCTCCTGCGAACGCAGGAGCCCAGGGCGGCCTAGCGCTGCCCCACTCTGGGTTCCCGCTTTCGCGGGAA
CGCACAAAGGATGCGGATGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTELTNLTVAQIRDGHRAGDFSAVEVAEAFNANVAAAKALNAFIVETPDLAIEAAKAADADRAAGTLKPLSGVPIGMKDL
FCTNGVQTTAASHMLEGFVPRYESTVSQKLWDAGAGMLGKLNLDQFAMGSSNETSYFGNVISPWRRNDGGNAALAPGGSS
GGSSAAIAARLCPAATGTDTGGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQAGPMARTVRDCAIMLENMAGFDP
KDATSLDLPVPNWEAALSSDLRGKTVGIPREYRLEGIDPDIDAMWDAGIAMLKDAEAAVVEISLPHTKYALPAYYIIAPA
EASSNLARYDGVRYGLRDLPQGAGLQDMYAATRAEGFGAEVKRRIMIGTYVLSAGFYDAYYTQAQKVRTLIARDFEAAFG
SCDVILAPTAPSAAFGLGEKMADPLAMYLNDVFAVPASLAGLPAMSVPAALNREGLPLGLQIIGKPFDEQGVLNAGLAIE
ERAGFTARAEKWW

Sequences:

>Translated_493_residues
MTELTNLTVAQIRDGHRAGDFSAVEVAEAFNANVAAAKALNAFIVETPDLAIEAAKAADADRAAGTLKPLSGVPIGMKDL
FCTNGVQTTAASHMLEGFVPRYESTVSQKLWDAGAGMLGKLNLDQFAMGSSNETSYFGNVISPWRRNDGGNAALAPGGSS
GGSSAAIAARLCPAATGTDTGGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQAGPMARTVRDCAIMLENMAGFDP
KDATSLDLPVPNWEAALSSDLRGKTVGIPREYRLEGIDPDIDAMWDAGIAMLKDAEAAVVEISLPHTKYALPAYYIIAPA
EASSNLARYDGVRYGLRDLPQGAGLQDMYAATRAEGFGAEVKRRIMIGTYVLSAGFYDAYYTQAQKVRTLIARDFEAAFG
SCDVILAPTAPSAAFGLGEKMADPLAMYLNDVFAVPASLAGLPAMSVPAALNREGLPLGLQIIGKPFDEQGVLNAGLAIE
ERAGFTARAEKWW
>Mature_492_residues
TELTNLTVAQIRDGHRAGDFSAVEVAEAFNANVAAAKALNAFIVETPDLAIEAAKAADADRAAGTLKPLSGVPIGMKDLF
CTNGVQTTAASHMLEGFVPRYESTVSQKLWDAGAGMLGKLNLDQFAMGSSNETSYFGNVISPWRRNDGGNAALAPGGSSG
GSSAAIAARLCPAATGTDTGGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQAGPMARTVRDCAIMLENMAGFDPK
DATSLDLPVPNWEAALSSDLRGKTVGIPREYRLEGIDPDIDAMWDAGIAMLKDAEAAVVEISLPHTKYALPAYYIIAPAE
ASSNLARYDGVRYGLRDLPQGAGLQDMYAATRAEGFGAEVKRRIMIGTYVLSAGFYDAYYTQAQKVRTLIARDFEAAFGS
CDVILAPTAPSAAFGLGEKMADPLAMYLNDVFAVPASLAGLPAMSVPAALNREGLPLGLQIIGKPFDEQGVLNAGLAIEE
RAGFTARAEKWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=490, Percent_Identity=39.7959183673469, Blast_Score=328, Evalue=9e-90,
Organism=Homo sapiens, GI195972892, Length=503, Percent_Identity=25.0497017892644, Blast_Score=101, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=434, Percent_Identity=38.9400921658986, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=25.6, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=270, Percent_Identity=27.4074074074074, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=427, Percent_Identity=35.3629976580796, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=417, Percent_Identity=29.2565947242206, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320448, Length=204, Percent_Identity=30.8823529411765, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648113, Length=491, Percent_Identity=38.0855397148676, Blast_Score=302, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24652985, Length=470, Percent_Identity=25.9574468085106, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922090, Length=470, Percent_Identity=25.9574468085106, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652981, Length=470, Percent_Identity=25.9574468085106, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652983, Length=470, Percent_Identity=25.9574468085106, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=23.8834951456311, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24644968, Length=513, Percent_Identity=23.0019493177388, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_SPHAL (Q1GUW3)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615892.1
- ProteinModelPortal:   Q1GUW3
- SMR:   Q1GUW3
- GeneID:   4080049
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0841
- NMPDR:   fig|317655.9.peg.787
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   SALA317655:SALA_0841-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51841; Mature: 51710

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 159-159 ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELTNLTVAQIRDGHRAGDFSAVEVAEAFNANVAAAKALNAFIVETPDLAIEAAKAADA
CCCCCCEEHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHCEEEEECCCHHHHHHHHCCC
DRAAGTLKPLSGVPIGMKDLFCTNGVQTTAASHMLEGFVPRYESTVSQKLWDAGAGMLGK
HHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LNLDQFAMGSSNETSYFGNVISPWRRNDGGNAALAPGGSSGGSSAAIAARLCPAATGTDT
CCHHHHCCCCCCCCCCCCCCCCCHHCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCC
GGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQAGPMARTVRDCAIMLENMAGFDP
CCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
KDATSLDLPVPNWEAALSSDLRGKTVGIPREYRLEGIDPDIDAMWDAGIAMLKDAEAAVV
CCCCEECCCCCCHHHHHHCCCCCCEECCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEE
EISLPHTKYALPAYYIIAPAEASSNLARYDGVRYGLRDLPQGAGLQDMYAATRAEGFGAE
EEECCCCCCCCCEEEEEEECCCCCCHHHHCCHHHHHHHCCCCCCHHHHHHHHHCCCCCHH
VKRRIMIGTYVLSAGFYDAYYTQAQKVRTLIARDFEAAFGSCDVILAPTAPSAAFGLGEK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCHH
MADPLAMYLNDVFAVPASLAGLPAMSVPAALNREGLPLGLQIIGKPFDEQGVLNAGLAIE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEHHEECCCCCCCCCCCCCCEEE
ERAGFTARAEKWW
CCCCCCCCCCCCC
>Mature Secondary Structure 
TELTNLTVAQIRDGHRAGDFSAVEVAEAFNANVAAAKALNAFIVETPDLAIEAAKAADA
CCCCCEEHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHCEEEEECCCHHHHHHHHCCC
DRAAGTLKPLSGVPIGMKDLFCTNGVQTTAASHMLEGFVPRYESTVSQKLWDAGAGMLGK
HHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LNLDQFAMGSSNETSYFGNVISPWRRNDGGNAALAPGGSSGGSSAAIAARLCPAATGTDT
CCHHHHCCCCCCCCCCCCCCCCCHHCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCC
GGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQAGPMARTVRDCAIMLENMAGFDP
CCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
KDATSLDLPVPNWEAALSSDLRGKTVGIPREYRLEGIDPDIDAMWDAGIAMLKDAEAAVV
CCCCEECCCCCCHHHHHHCCCCCCEECCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEE
EISLPHTKYALPAYYIIAPAEASSNLARYDGVRYGLRDLPQGAGLQDMYAATRAEGFGAE
EEECCCCCCCCCEEEEEEECCCCCCHHHHCCHHHHHHHCCCCCCHHHHHHHHHCCCCCHH
VKRRIMIGTYVLSAGFYDAYYTQAQKVRTLIARDFEAAFGSCDVILAPTAPSAAFGLGEK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCHH
MADPLAMYLNDVFAVPASLAGLPAMSVPAALNREGLPLGLQIIGKPFDEQGVLNAGLAIE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEHHEECCCCCCCCCCCCCCEEE
ERAGFTARAEKWW
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA