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The map label for this gene is tig [H]

Identifier: 103486333

GI number: 103486333

Start: 850774

End: 852408

Strand: Direct

Name: tig [H]

Synonym: Sala_0843

Alternate gene names: 103486333

Gene position: 850774-852408 (Clockwise)

Preceding gene: 103486332

Following gene: 103486335

Centisome position: 25.43

GC content: 64.53

Gene sequence:

>1635_bases
TTGAGCAAGGATAAGACCAAGGCAATGAAGACCGTCGAAACGCTGAACGAAGGCCTGAAGCGCGAATATCGCGTCACCAT
CACCGCCAAGGATATCGACGCGCGCGTCGACGAAGAGGTGAAGAGCATCGCCCCGACCGTCCGCATGCCCGGTTTCCGCC
CCGGCAAGGTGCCGCCCAACCTCATTCGCAAGATGCACGGCGCCGCGCTGTCGGCCGACGCGCTCAACAAGGCGATCGAC
GCCGGCGTGCGCGACCTGATGGCGAAGGAAAAGCTGCGCCCGGCGCTCCAGCCCGCGGTGACGCTGGCCGACGGTTATGA
AGCCGGCAAGGATGCCGAGTTGACCGTCGCGCTGGAAGTGCTGCCGGAAATCGAAACGCCGTCGATCGACGGGCTGAAGC
TCGAGCGGCTGACCGTTCCCGCCGACGACAAGGCGGTGATGGCGAAGATCGAGGAATTCGCCGCGCAGATGAAGCGCTTC
GAAGAAGCGCCGAAGACCAGGAAGGCCGCGACCGGCGATCAGGTCATCATCGACTTTGCCGGCAGCGTCGACGGCACCCC
CTTCGACGGCGGCACGGGCGAGGATATGGCGGTCGAAATCGGTTCGGGCCAGCTGATCCCCGGTTTCGAGGACCAGCTCG
TCGGCGTCAAGGCGGGCGACGAAAAAACGTTGAAGGTCACGTTCCCGGAAGACTATCCGGTCGAGACCCTGAAGGGCAAG
CTGGCCGAATTCGCCGTGACGGTGAAGGAAGTGAAGGTTCCCGCCGCGACGAAGATCGACGACGAATTTGCCAAGTCGCT
CGGCCTCGAAAGCCTCGACAAGCTCAAGGAGCTGATGAAGGATCAGGTCGAGCAGGAACTCAACGGCCTGACCCGCACCC
ATATGAAGCGCAAGCTGCTCGACCAGCTCGCCGCCGCCCATGACTTCGAGGTGCCGCCGACGATGGTCGAGGCCGAGTTC
AACCAGATCTGGCAGCAGCTCGAGCATGAAGCGAGCCACGAGGAAGATCCCGAAGCGGCGAAGGCCGAGCTGGAGAACGA
CCGCGAGGAATATCGTGCCATCGCGGTGCGCCGCGTCCGCCTGGGCCTGCTCCTCTCCGAAATCGGCCAGGCGCATGGCG
TGCAGGTCTCTCAGCAGGAGATGCAGCGCCTGATCATGCAGGCGGCGCAGCAGTATCGGCCCGAGGATCGCCAGCGGTTC
GTCGAATATGTCCAGCAGGACGCGCTCGCCGCCGCGCAGCTCCGCGCCCCGCTCTATGAAGACAAGGTCGTCGACTTCCT
GTTTGAAAAGGCCGAAATCACCGACCGCGAAACGACGCGCGAGGAACTGGAGGCGGCGATCGAGGCCGACGACGACAGCG
GTCATGTCCACGGACCGGGCTGCGGCCACGACCATGACGAAAAACCGGCCAAGAAGGCGGCGGCGAAGAAGCCCGCCACC
AAGAAGGAAGCGGTGAAGGACGAGGCCAAGGCCGAGGAAGCCCCCGCCAAGAAGGCGCCGGCGAAAAAGGCCGAGCCCAA
GGCCGAAGCCAAACCCGCCGCGGCAAAGAAGGCCGCGCCGGCCAAGGCTGCGGCCGAGGAAAAGGCCGAGCCAGCGAAGA
AGGCGCCCGCCAAAAAGGCTGCGGCGAAGAAATAG

Upstream 100 bases:

>100_bases
AGATGTGGGGGTTCGAGTCCCTTCGCCCGCACCACCTTCGCGAGCAGCAAGCCGGGACAAGGGGAACGCCGCCATTTGGC
CGGGGTTCGGACAAGAGATT

Downstream 100 bases:

>100_bases
GTTTCGGCTGTCGATCGACAAAAGGCCGGGTGGAGGATGCTCCGCCCGGCCTTTTTGTTTCAGAACGGCGCCTCCGCGCA
GGCACGGGCCGCTATCGGTA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSKDKTKAMKTVETLNEGLKREYRVTITAKDIDARVDEEVKSIAPTVRMPGFRPGKVPPNLIRKMHGAALSADALNKAID
AGVRDLMAKEKLRPALQPAVTLADGYEAGKDAELTVALEVLPEIETPSIDGLKLERLTVPADDKAVMAKIEEFAAQMKRF
EEAPKTRKAATGDQVIIDFAGSVDGTPFDGGTGEDMAVEIGSGQLIPGFEDQLVGVKAGDEKTLKVTFPEDYPVETLKGK
LAEFAVTVKEVKVPAATKIDDEFAKSLGLESLDKLKELMKDQVEQELNGLTRTHMKRKLLDQLAAAHDFEVPPTMVEAEF
NQIWQQLEHEASHEEDPEAAKAELENDREEYRAIAVRRVRLGLLLSEIGQAHGVQVSQQEMQRLIMQAAQQYRPEDRQRF
VEYVQQDALAAAQLRAPLYEDKVVDFLFEKAEITDRETTREELEAAIEADDDSGHVHGPGCGHDHDEKPAKKAAAKKPAT
KKEAVKDEAKAEEAPAKKAPAKKAEPKAEAKPAAAKKAAPAKAAAEEKAEPAKKAPAKKAAAKK

Sequences:

>Translated_544_residues
MSKDKTKAMKTVETLNEGLKREYRVTITAKDIDARVDEEVKSIAPTVRMPGFRPGKVPPNLIRKMHGAALSADALNKAID
AGVRDLMAKEKLRPALQPAVTLADGYEAGKDAELTVALEVLPEIETPSIDGLKLERLTVPADDKAVMAKIEEFAAQMKRF
EEAPKTRKAATGDQVIIDFAGSVDGTPFDGGTGEDMAVEIGSGQLIPGFEDQLVGVKAGDEKTLKVTFPEDYPVETLKGK
LAEFAVTVKEVKVPAATKIDDEFAKSLGLESLDKLKELMKDQVEQELNGLTRTHMKRKLLDQLAAAHDFEVPPTMVEAEF
NQIWQQLEHEASHEEDPEAAKAELENDREEYRAIAVRRVRLGLLLSEIGQAHGVQVSQQEMQRLIMQAAQQYRPEDRQRF
VEYVQQDALAAAQLRAPLYEDKVVDFLFEKAEITDRETTREELEAAIEADDDSGHVHGPGCGHDHDEKPAKKAAAKKPAT
KKEAVKDEAKAEEAPAKKAPAKKAEPKAEAKPAAAKKAAPAKAAAEEKAEPAKKAPAKKAAAKK
>Mature_543_residues
SKDKTKAMKTVETLNEGLKREYRVTITAKDIDARVDEEVKSIAPTVRMPGFRPGKVPPNLIRKMHGAALSADALNKAIDA
GVRDLMAKEKLRPALQPAVTLADGYEAGKDAELTVALEVLPEIETPSIDGLKLERLTVPADDKAVMAKIEEFAAQMKRFE
EAPKTRKAATGDQVIIDFAGSVDGTPFDGGTGEDMAVEIGSGQLIPGFEDQLVGVKAGDEKTLKVTFPEDYPVETLKGKL
AEFAVTVKEVKVPAATKIDDEFAKSLGLESLDKLKELMKDQVEQELNGLTRTHMKRKLLDQLAAAHDFEVPPTMVEAEFN
QIWQQLEHEASHEEDPEAAKAELENDREEYRAIAVRRVRLGLLLSEIGQAHGVQVSQQEMQRLIMQAAQQYRPEDRQRFV
EYVQQDALAAAQLRAPLYEDKVVDFLFEKAEITDRETTREELEAAIEADDDSGHVHGPGCGHDHDEKPAKKAAAKKPATK
KEAVKDEAKAEEAPAKKAPAKKAEPKAEAKPAAAKKAAPAKAAAEEKAEPAKKAPAKKAAAKK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=435, Percent_Identity=33.5632183908046, Blast_Score=229, Evalue=4e-61,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 59488; Mature: 59357

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDKTKAMKTVETLNEGLKREYRVTITAKDIDARVDEEVKSIAPTVRMPGFRPGKVPPN
CCCHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHCCHHCCCCCCCCCCCHH
LIRKMHGAALSADALNKAIDAGVRDLMAKEKLRPALQPAVTLADGYEAGKDAELTVALEV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
LPEIETPSIDGLKLERLTVPADDKAVMAKIEEFAAQMKRFEEAPKTRKAATGDQVIIDFA
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEEC
GSVDGTPFDGGTGEDMAVEIGSGQLIPGFEDQLVGVKAGDEKTLKVTFPEDYPVETLKGK
CCCCCCCCCCCCCCCEEEEECCCCCCCCCHHCEEEEECCCCCEEEEECCCCCCHHHHHHH
LAEFAVTVKEVKVPAATKIDDEFAKSLGLESLDKLKELMKDQVEQELNGLTRTHMKRKLL
HHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQLAAAHDFEVPPTMVEAEFNQIWQQLEHEASHEEDPEAAKAELENDREEYRAIAVRRVR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGLLLSEIGQAHGVQVSQQEMQRLIMQAAQQYRPEDRQRFVEYVQQDALAAAQLRAPLYE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
DKVVDFLFEKAEITDRETTREELEAAIEADDDSGHVHGPGCGHDHDEKPAKKAAAKKPAT
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
KKEAVKDEAKAEEAPAKKAPAKKAEPKAEAKPAAAKKAAPAKAAAEEKAEPAKKAPAKKA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCHHHHCCHHHH
AAKK
CCCC
>Mature Secondary Structure 
SKDKTKAMKTVETLNEGLKREYRVTITAKDIDARVDEEVKSIAPTVRMPGFRPGKVPPN
CCHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHCCHHCCCCCCCCCCCHH
LIRKMHGAALSADALNKAIDAGVRDLMAKEKLRPALQPAVTLADGYEAGKDAELTVALEV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
LPEIETPSIDGLKLERLTVPADDKAVMAKIEEFAAQMKRFEEAPKTRKAATGDQVIIDFA
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEEC
GSVDGTPFDGGTGEDMAVEIGSGQLIPGFEDQLVGVKAGDEKTLKVTFPEDYPVETLKGK
CCCCCCCCCCCCCCCEEEEECCCCCCCCCHHCEEEEECCCCCEEEEECCCCCCHHHHHHH
LAEFAVTVKEVKVPAATKIDDEFAKSLGLESLDKLKELMKDQVEQELNGLTRTHMKRKLL
HHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQLAAAHDFEVPPTMVEAEFNQIWQQLEHEASHEEDPEAAKAELENDREEYRAIAVRRVR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGLLLSEIGQAHGVQVSQQEMQRLIMQAAQQYRPEDRQRFVEYVQQDALAAAQLRAPLYE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
DKVVDFLFEKAEITDRETTREELEAAIEADDDSGHVHGPGCGHDHDEKPAKKAAAKKPAT
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
KKEAVKDEAKAEEAPAKKAPAKKAEPKAEAKPAAAKKAAPAKAAAEEKAEPAKKAPAKKA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCHHHHCCHHHH
AAKK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA