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The map label for this gene is dnaB [H]

Identifier: 103486258

GI number: 103486258

Start: 773221

End: 774732

Strand: Direct

Name: dnaB [H]

Synonym: Sala_0766

Alternate gene names: 103486258

Gene position: 773221-774732 (Clockwise)

Preceding gene: 103486251

Following gene: 103486266

Centisome position: 23.11

GC content: 64.42

Gene sequence:

>1512_bases
ATGCCCGAACTCGCCCTTATTCCGACCCCGGCAACCGCCGGGGATGAGCGCCAATTACCACGCAATATCGAGGCCGAGGC
CGCGTTTCTGGGCGCGATCCTGATCGACAATCGAGTCGTCGAGGATCTGCCCGTCCAGCTCACCCCCGACCATTTCTTCG
AACCACTGCACGGCCGCATCTTTCAGCAGACGATGGCGCTGATCGAACGCAACAGCATTGCAACGCCGGTGACGCTCAAG
CCCTATTTCGAGGCCGACGAGGCGATGAAGGCGGTTGGCGGCGTCGGCTATCTGGCGCAGCTGACGGGCAGCGGCGCGGG
GCTGATCGGCGCGCGCGATTTCGCCAAGCAGATTTACGACCTCGCGCTGCTTCGCGAACTCGCGGGGGTCGGGCGGACGC
TCGTCGAGAATGCGCTCGACACCAGCGAGCGTGTCGATCCGCAGGCGCAGATCGAGGAGGCCGAGACGGCGCTTTATCGC
GTCGCGGGCGGCGAGGCCGAAATGGGGTCGGTCAAAAGCTTCAGCCAGGCGAGCCTCACCGCGCTGCAGGCGGCCGAGCG
CGCGCTCAATTCGGGTGGCCATCTGTCGGGCATCACCACCGGCATCGCGAGCATGAACGCCAAGATCGGCGGGATGCACA
ATTCCGACCTGATGATCCTCGCGGGGCGGCCCGGCATGGGCAAGACGTCGCTCGCGACCAACATCGCCTATAATGCCGCC
GAACGCTGGCGCCGCGACGAGGAAGACGGCATTCCGCCCGAAAAGAATATGGGCGCCAAGGTCGCTTTCTTCAGCCTCGA
AATGTCCGCCGACCAGCTGGCAACGCGCGTGCTCGCCGAACAGTCGGGGGTGTCGGGCGAGGCGCTGCGCATGGGCAAGA
TCAGCAAGGAACAGTTCCAGCAGCTGTCGCGCGCCGCACAGACGCTCCAGACGCTGCCGCTGTATATCGACGACACGCCC
GGCCTGACGATCGCGGGGCTGCGCACGCGCGCGCGGCGGCTCCAGCGGCGCCACGGCATCGGCTTCATCATCGTCGATTA
TCTTCAGCTGTTGCAGGGATCGTCGCGCAGCGGCGACAACCGCGTGCAGGAAATATCAGAAATATCCCGCGGTTTGAAGA
CGTTGGCGAAGGAACTCAATGTCCCGGTGATGGCGCTGTCGCAGCTGAGCCGCCAGGTCGAAAGCCGCGAGGACAAGCGC
CCGCAACTCTCCGACCTGCGTGAATCGGGGTCGATCGAGCAGGATGCCGACATGGTGCTCTTTGTGTTCCGCGAGGATTA
TTATGTCGCGGCGCGCGAACCCAAGCGCCCGGTCGAGGGCGACGATGTGAAGATACACGCGGCGCACGAGGAATGGGCGG
CCGAAATGGAGCGCGTCTTCGGCCTCGCCGAAGTCATCGTCGCCAAATCGCGTCACGGTTCGACCGGCAAGATCCGCCTG
CACTTCGAGGCGAAGACGACGAAGTTCAGCGACCTCGCCGACGACAGCATGGCGTATGAGGATTATGAGTGA

Upstream 100 bases:

>100_bases
ATAATTGCAGCGAAATTATCCCCAGAAGCACCCCCGGCCGGGATGGATCGGAATCGCCAATCCGGTTGCGCGCAACGGGT
GATCAGCGCATGATGACGCC

Downstream 100 bases:

>100_bases
GTGACGTCTGCGTTAAATCGTCATCCCAGCGAAAGCTGGGATCGCTCGCGGGATAAAATGAATGATGGCGGGCCCGGCGT
TCGCCGGGGCGACTTCAAAG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MPELALIPTPATAGDERQLPRNIEAEAAFLGAILIDNRVVEDLPVQLTPDHFFEPLHGRIFQQTMALIERNSIATPVTLK
PYFEADEAMKAVGGVGYLAQLTGSGAGLIGARDFAKQIYDLALLRELAGVGRTLVENALDTSERVDPQAQIEEAETALYR
VAGGEAEMGSVKSFSQASLTALQAAERALNSGGHLSGITTGIASMNAKIGGMHNSDLMILAGRPGMGKTSLATNIAYNAA
ERWRRDEEDGIPPEKNMGAKVAFFSLEMSADQLATRVLAEQSGVSGEALRMGKISKEQFQQLSRAAQTLQTLPLYIDDTP
GLTIAGLRTRARRLQRRHGIGFIIVDYLQLLQGSSRSGDNRVQEISEISRGLKTLAKELNVPVMALSQLSRQVESREDKR
PQLSDLRESGSIEQDADMVLFVFREDYYVAAREPKRPVEGDDVKIHAAHEEWAAEMERVFGLAEVIVAKSRHGSTGKIRL
HFEAKTTKFSDLADDSMAYEDYE

Sequences:

>Translated_503_residues
MPELALIPTPATAGDERQLPRNIEAEAAFLGAILIDNRVVEDLPVQLTPDHFFEPLHGRIFQQTMALIERNSIATPVTLK
PYFEADEAMKAVGGVGYLAQLTGSGAGLIGARDFAKQIYDLALLRELAGVGRTLVENALDTSERVDPQAQIEEAETALYR
VAGGEAEMGSVKSFSQASLTALQAAERALNSGGHLSGITTGIASMNAKIGGMHNSDLMILAGRPGMGKTSLATNIAYNAA
ERWRRDEEDGIPPEKNMGAKVAFFSLEMSADQLATRVLAEQSGVSGEALRMGKISKEQFQQLSRAAQTLQTLPLYIDDTP
GLTIAGLRTRARRLQRRHGIGFIIVDYLQLLQGSSRSGDNRVQEISEISRGLKTLAKELNVPVMALSQLSRQVESREDKR
PQLSDLRESGSIEQDADMVLFVFREDYYVAAREPKRPVEGDDVKIHAAHEEWAAEMERVFGLAEVIVAKSRHGSTGKIRL
HFEAKTTKFSDLADDSMAYEDYE
>Mature_502_residues
PELALIPTPATAGDERQLPRNIEAEAAFLGAILIDNRVVEDLPVQLTPDHFFEPLHGRIFQQTMALIERNSIATPVTLKP
YFEADEAMKAVGGVGYLAQLTGSGAGLIGARDFAKQIYDLALLRELAGVGRTLVENALDTSERVDPQAQIEEAETALYRV
AGGEAEMGSVKSFSQASLTALQAAERALNSGGHLSGITTGIASMNAKIGGMHNSDLMILAGRPGMGKTSLATNIAYNAAE
RWRRDEEDGIPPEKNMGAKVAFFSLEMSADQLATRVLAEQSGVSGEALRMGKISKEQFQQLSRAAQTLQTLPLYIDDTPG
LTIAGLRTRARRLQRRHGIGFIIVDYLQLLQGSSRSGDNRVQEISEISRGLKTLAKELNVPVMALSQLSRQVESREDKRP
QLSDLRESGSIEQDADMVLFVFREDYYVAAREPKRPVEGDDVKIHAAHEEWAAEMERVFGLAEVIVAKSRHGSTGKIRLH
FEAKTTKFSDLADDSMAYEDYE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=478, Percent_Identity=36.1924686192469, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55013; Mature: 54882

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELALIPTPATAGDERQLPRNIEAEAAFLGAILIDNRVVEDLPVQLTPDHFFEPLHGRI
CCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCEECCHHHHCHHHHHH
FQQTMALIERNSIATPVTLKPYFEADEAMKAVGGVGYLAQLTGSGAGLIGARDFAKQIYD
HHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHH
LALLRELAGVGRTLVENALDTSERVDPQAQIEEAETALYRVAGGEAEMGSVKSFSQASLT
HHHHHHHHHCCHHHHHHHHCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ALQAAERALNSGGHLSGITTGIASMNAKIGGMHNSDLMILAGRPGMGKTSLATNIAYNAA
HHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
ERWRRDEEDGIPPEKNMGAKVAFFSLEMSADQLATRVLAEQSGVSGEALRMGKISKEQFQ
HHHCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHH
QLSRAAQTLQTLPLYIDDTPGLTIAGLRTRARRLQRRHGIGFIIVDYLQLLQGSSRSGDN
HHHHHHHHHHHCCEEEECCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHH
RVQEISEISRGLKTLAKELNVPVMALSQLSRQVESREDKRPQLSDLRESGSIEQDADMVL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEE
FVFREDYYVAAREPKRPVEGDDVKIHAAHEEWAAEMERVFGLAEVIVAKSRHGSTGKIRL
EEEECCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
HFEAKTTKFSDLADDSMAYEDYE
EEEECCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PELALIPTPATAGDERQLPRNIEAEAAFLGAILIDNRVVEDLPVQLTPDHFFEPLHGRI
CCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCEECCHHHHCHHHHHH
FQQTMALIERNSIATPVTLKPYFEADEAMKAVGGVGYLAQLTGSGAGLIGARDFAKQIYD
HHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHH
LALLRELAGVGRTLVENALDTSERVDPQAQIEEAETALYRVAGGEAEMGSVKSFSQASLT
HHHHHHHHHCCHHHHHHHHCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ALQAAERALNSGGHLSGITTGIASMNAKIGGMHNSDLMILAGRPGMGKTSLATNIAYNAA
HHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
ERWRRDEEDGIPPEKNMGAKVAFFSLEMSADQLATRVLAEQSGVSGEALRMGKISKEQFQ
HHHCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHH
QLSRAAQTLQTLPLYIDDTPGLTIAGLRTRARRLQRRHGIGFIIVDYLQLLQGSSRSGDN
HHHHHHHHHHHCCEEEECCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHH
RVQEISEISRGLKTLAKELNVPVMALSQLSRQVESREDKRPQLSDLRESGSIEQDADMVL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEE
FVFREDYYVAAREPKRPVEGDDVKIHAAHEEWAAEMERVFGLAEVIVAKSRHGSTGKIRL
EEEECCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
HFEAKTTKFSDLADDSMAYEDYE
EEEECCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA