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The map label for this gene is pit [H]

Identifier: 103486210

GI number: 103486210

Start: 723152

End: 724165

Strand: Direct

Name: pit [H]

Synonym: Sala_0717

Alternate gene names: 103486210

Gene position: 723152-724165 (Clockwise)

Preceding gene: 103486209

Following gene: 103486212

Centisome position: 21.62

GC content: 64.69

Gene sequence:

>1014_bases
ATGCACGAACTCGCTTTCCCGCTCCTCGTCGGCCTCGTCATCCTTGCGCTGGCGTTCGATTTCCTGAACGGGCTGCACGA
CGCGGCGAACAGCATCGCGACGGTTGTCGCGACGCGATTGCTGCGCCCGGTTCAGGCGGTGGTGTTCGCGGCTTTCTTCA
ACTTCGCCGCCTATTTCCTGAGCCTCGCCTTTCCGGCGCTGCACAAGGTTGCCGAGACGATCGGGGCGGGGTTGATCGAC
AAGGATCTGGTCACTCCCGCGGTCGTGTTCGGCGCGCTGGTCGGCGCGATGGTCTGGAACGTCGTGACCTGGCTGAAGGG
CATTCCATCCTCGTCGAGCCATGCGCTGGTGGGCGGCATCGTCGGCGCAGGCGTCGCCCATGCGGGGTTCGAGGGTATCC
AGTGGAGCGGGCTCAACAAGACGGTCATTGCGATCTTCCTGTCGCCGATGCTCGGCATGTTCCTCGCGATGCTGGTGATG
CTGGCGAGCAGCTGGGCGCTGCACCGCGCGACCGCGAAATTCGCCGAATCGAGCTTTCGCACGCTCCACCTCTTTTCGTC
CGCCGCCTATTCGCTGAGCCACGGGCTCAACGATGCGCAAAAGACGATGGGGATCATCGCGGTGCTGCTCTATTCGACCG
GTTATCTGTCGGGCGAGTTCCATGTGCCGCACTGGGTCGCGTTCGCCTGCTATATCGCGATCGCGCTCGGCACGCTGTCG
GGCGGGTGGAAGATCATCGAGACGATGGGCGGCCGCATCACCAAATTGTCGCACCATCAGGGCTTCGCCGCCTCGACGGG
CGGGTCGATCATGGTGTTCACCGCCAGCCTGCTCGGCATCCCGGTATCGACGACGCACACGATTACCGGCAGCATCATCG
GCGCCGGCGTCGCGCGGCGGACGAGCGCGGTGCGCTGGGGCGTGGCGGGCAATGTCATCGCGGCCTGGTTTATCACCATT
CCCGCAAGTGCAGCGGTGGCGGCGGTCTTCTATGCGCTGACGCGGCTGTTCTGA

Upstream 100 bases:

>100_bases
GAAGCCGGAAGTCGAGATGCCCATCCGCATGGCGCTGGCCAGAAGGGTGTCTCGACTTCGCTCGACACGAGCGGGTTTCT
GCGGTCGGGAAGATAGGTCC

Downstream 100 bases:

>100_bases
GCGACTGCAGGCCAAACAAAAATGGCTTGATTATCAGAACTTCACTCTGAACTCGCTGTATCGTGGAGCGTGCAGGCGCC
ATTCCACAGGGCTATGCGAC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MHELAFPLLVGLVILALAFDFLNGLHDAANSIATVVATRLLRPVQAVVFAAFFNFAAYFLSLAFPALHKVAETIGAGLID
KDLVTPAVVFGALVGAMVWNVVTWLKGIPSSSSHALVGGIVGAGVAHAGFEGIQWSGLNKTVIAIFLSPMLGMFLAMLVM
LASSWALHRATAKFAESSFRTLHLFSSAAYSLSHGLNDAQKTMGIIAVLLYSTGYLSGEFHVPHWVAFACYIAIALGTLS
GGWKIIETMGGRITKLSHHQGFAASTGGSIMVFTASLLGIPVSTTHTITGSIIGAGVARRTSAVRWGVAGNVIAAWFITI
PASAAVAAVFYALTRLF

Sequences:

>Translated_337_residues
MHELAFPLLVGLVILALAFDFLNGLHDAANSIATVVATRLLRPVQAVVFAAFFNFAAYFLSLAFPALHKVAETIGAGLID
KDLVTPAVVFGALVGAMVWNVVTWLKGIPSSSSHALVGGIVGAGVAHAGFEGIQWSGLNKTVIAIFLSPMLGMFLAMLVM
LASSWALHRATAKFAESSFRTLHLFSSAAYSLSHGLNDAQKTMGIIAVLLYSTGYLSGEFHVPHWVAFACYIAIALGTLS
GGWKIIETMGGRITKLSHHQGFAASTGGSIMVFTASLLGIPVSTTHTITGSIIGAGVARRTSAVRWGVAGNVIAAWFITI
PASAAVAAVFYALTRLF
>Mature_337_residues
MHELAFPLLVGLVILALAFDFLNGLHDAANSIATVVATRLLRPVQAVVFAAFFNFAAYFLSLAFPALHKVAETIGAGLID
KDLVTPAVVFGALVGAMVWNVVTWLKGIPSSSSHALVGGIVGAGVAHAGFEGIQWSGLNKTVIAIFLSPMLGMFLAMLVM
LASSWALHRATAKFAESSFRTLHLFSSAAYSLSHGLNDAQKTMGIIAVLLYSTGYLSGEFHVPHWVAFACYIAIALGTLS
GGWKIIETMGGRITKLSHHQGFAASTGGSIMVFTASLLGIPVSTTHTITGSIIGAGVARRTSAVRWGVAGNVIAAWFITI
PASAAVAAVFYALTRLF

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=159, Percent_Identity=32.0754716981132, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI5803173, Length=157, Percent_Identity=32.484076433121, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789360, Length=225, Percent_Identity=30.6666666666667, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789907, Length=225, Percent_Identity=32.4444444444444, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=28.3950617283951, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=26.5432098765432, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=179, Percent_Identity=27.9329608938547, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17539280, Length=163, Percent_Identity=27.6073619631902, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319773, Length=157, Percent_Identity=26.7515923566879, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356511, Length=177, Percent_Identity=27.1186440677966, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35333; Mature: 35333

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELAFPLLVGLVILALAFDFLNGLHDAANSIATVVATRLLRPVQAVVFAAFFNFAAYFL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAFPALHKVAETIGAGLIDKDLVTPAVVFGALVGAMVWNVVTWLKGIPSSSSHALVGGI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VGAGVAHAGFEGIQWSGLNKTVIAIFLSPMLGMFLAMLVMLASSWALHRATAKFAESSFR
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TLHLFSSAAYSLSHGLNDAQKTMGIIAVLLYSTGYLSGEFHVPHWVAFACYIAIALGTLS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCC
GGWKIIETMGGRITKLSHHQGFAASTGGSIMVFTASLLGIPVSTTHTITGSIIGAGVARR
CCHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHH
TSAVRWGVAGNVIAAWFITIPASAAVAAVFYALTRLF
HHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHELAFPLLVGLVILALAFDFLNGLHDAANSIATVVATRLLRPVQAVVFAAFFNFAAYFL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAFPALHKVAETIGAGLIDKDLVTPAVVFGALVGAMVWNVVTWLKGIPSSSSHALVGGI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VGAGVAHAGFEGIQWSGLNKTVIAIFLSPMLGMFLAMLVMLASSWALHRATAKFAESSFR
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TLHLFSSAAYSLSHGLNDAQKTMGIIAVLLYSTGYLSGEFHVPHWVAFACYIAIALGTLS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCC
GGWKIIETMGGRITKLSHHQGFAASTGGSIMVFTASLLGIPVSTTHTITGSIIGAGVARR
CCHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHH
TSAVRWGVAGNVIAAWFITIPASAAVAAVFYALTRLF
HHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]